BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0286.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC t... 798 0.0 gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC t... 796 0.0 gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC t... 273 2e-74 gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC t... 126 3e-30 gi|300312238|ref|YP_003776330.1| amino acid ABC transporter perm... 120 2e-28 >gi|300310522|ref|YP_003774614.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 798 bits (2060), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/404 (99%), Positives = 404/404 (100%) Query: 1 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV 60 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV Sbjct: 1 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV 60 Query: 61 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG 120 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG Sbjct: 61 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG 120 Query: 121 IHLSLWLIVPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 IHLSLWLIVPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT Sbjct: 121 IHLSLWLIVPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 Query: 181 NGPQGINLIDPIKVFGVSLAGEPGSGSMVQVFGMSMPSVNAYYFLFLLLCIGVIFFSVRL 240 NGPQGINLIDPIKVFGVSLAGEPGSGSMV+VFGMSMPSVNAYYFLFLLLCIGVIFFSVRL Sbjct: 181 NGPQGINLIDPIKVFGVSLAGEPGSGSMVKVFGMSMPSVNAYYFLFLLLCIGVIFFSVRL 240 Query: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF 300 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF Sbjct: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF 300 Query: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR 360 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR Sbjct: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR 360 Query: 361 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQVGTTGEVKA 404 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQVGTTGEVKA Sbjct: 361 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQVGTTGEVKA 404 >gi|300309520|ref|YP_003773612.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 404 Score = 796 bits (2057), Expect = 0.0, Method: Compositional matrix adjust. Identities = 402/404 (99%), Positives = 403/404 (99%) Query: 1 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV 60 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV Sbjct: 1 MALLTFDMKRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVV 60 Query: 61 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG 120 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG Sbjct: 61 VGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNG 120 Query: 121 IHLSLWLIVPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 IHLSLWLIVPISA LAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT Sbjct: 121 IHLSLWLIVPISAFLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNIT 180 Query: 181 NGPQGINLIDPIKVFGVSLAGEPGSGSMVQVFGMSMPSVNAYYFLFLLLCIGVIFFSVRL 240 NGPQGINLIDPIKVFGVSLAGEPGSGSMV+VFGMSMPSVNAYYFLFLLLCIGVIFFSVRL Sbjct: 181 NGPQGINLIDPIKVFGVSLAGEPGSGSMVKVFGMSMPSVNAYYFLFLLLCIGVIFFSVRL 240 Query: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF 300 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF Sbjct: 241 QDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESF 300 Query: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR 360 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR Sbjct: 301 SLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLR 360 Query: 361 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQVGTTGEVKA 404 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQVGTTGEVKA Sbjct: 361 QLLYGLAMVVIMLTRPAGLWPSPRHEDRPEADHTQVGTTGEVKA 404 >gi|300310993|ref|YP_003775085.1| branched-chain amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 408 Score = 273 bits (697), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 166/389 (42%), Positives = 230/389 (59%), Gaps = 61/389 (15%) Query: 9 KRNPQQARISLLLLLALMIVFPFVAQQFGNSWVRIMDVALLYIMLALGLNVVVGFAGLLD 68 + P+Q R ++++LL +V+PF + +V +M +AL+Y++L LGLN+VVGFAGLLD Sbjct: 74 RMQPRQQRAAVMILLMAALVWPFFGSR---GYVDVMTLALIYVVLGLGLNIVVGFAGLLD 130 Query: 69 LGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWLCGPEIVQNGIHLSLWLI 128 LGY+ FYA+GAY+ L N Y L+ W Sbjct: 131 LGYVGFYAVGAYTYAL-------------CNQY-------------------FGLTFWEC 158 Query: 129 VPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMNNLNAPVNITNGPQGINL 188 VP++A +ALFG LLG P L+LRGDYLAIVTLGFGEIIR+ +NN+ +IT GP G++ Sbjct: 159 VPLAAAASALFGFLLGFPVLRLRGDYLAIVTLGFGEIIRLLLNNMT---SITGGPDGVSG 215 Query: 189 IDPIKVFGVSLAGEP---GSGSMVQVFGMSMPS---VNAYYFLFLLLCIGVIFFSVRLQD 242 I VFG+ +A P G + Q+FG+S V YFL LL+ + F + RL Sbjct: 216 IPKPTVFGLVMARNPVTEGGTTFHQLFGLSYQGGHMVIFLYFLALLMVLFTYFVTSRLLR 275 Query: 243 SRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAFQGFVSPESFSL 302 +GRAW A+REDEIA +++GIN VKL AF +GA+F G+AG+ F A QG V+PESF+ Sbjct: 276 MPMGRAWEALREDEIACRSLGINPTKVKLSAFTLGAAFAGLAGSFFAARQGLVTPESFTF 335 Query: 303 TESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFGKVWIDAEVLRQL 362 ES +LA+VVLGG+G GV+L ++L LPE+ R E R L Sbjct: 336 IESALILAIVVLGGMGSQLGVILAAILLTVLPELARSFAE----------------YRML 379 Query: 363 LYGLAMVVIMLTRPAGLWPSPR-HEDRPE 390 ++GL MV++M+ RP GL P+ R H + P+ Sbjct: 380 IFGLVMVLMMMWRPQGLLPATRPHVELPQ 408 >gi|300311115|ref|YP_003775207.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 655 Score = 126 bits (317), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 86/308 (27%), Positives = 158/308 (51%), Gaps = 54/308 (17%) Query: 40 WVRIMDVALLYIMLALGLNVVVGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVN 99 ++ +++ L+Y +L GL++VVG+ G + LG+ + IG+Y+AG+L Sbjct: 28 YLHLIETILIYAILLFGLDIVVGYTGQVSLGHAGLFGIGSYAAGVL-------------- 73 Query: 100 TYPSVGNFLVWLCGPEIVQNGIHLSLWLIVPISALLAALFGALLGAPTLKLRGDYLAIVT 159 + +G + +++ VP + + A+FGA+L P L++ G YLA+VT Sbjct: 74 -FFKLG-----------------MPIFVTVPAAIAVTAVFGAILALPALRVTGPYLAMVT 115 Query: 160 LGFGEIIRIFMNNLNAPVNITNGPQGINLIDPIKVFGVSLAGEPGSGSMVQVFGMSMPSV 219 L FG II+I +N + +T GP GI L P+ + G M V Sbjct: 116 LAFGTIIQILINEMTF---LTEGPLGIKLQKPM------------------IGGDKMSEV 154 Query: 220 NAYYFLFLLLCIGVIFFSVRLQDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGAS 279 +Y + +L+ + ++ R+ S LGRA+ A+R+ IA+ MG++ K+ AF + A+ Sbjct: 155 QFFYLVAVLMVLSMLVVH-RILKSNLGRAFQALRDSPIASDCMGVSVYRYKVYAFIISAA 213 Query: 280 FGGVAGAMFGAFQGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRH 339 G++GA++ + ++SP +++ +I L V++GG G ++G +I+ LP +L Sbjct: 214 LAGLSGALYAYSEEYISPNTYNFELTIMFLLAVIMGGRKSRIGSLIGALIVVMLPSLLSD 273 Query: 340 VVEPVQMA 347 + Q+A Sbjct: 274 IALFRQIA 281 >gi|300312238|ref|YP_003776330.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 120 bits (302), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 101/299 (33%), Positives = 149/299 (49%), Gaps = 66/299 (22%) Query: 52 MLALGLNVVVGFAGLLDLGYIAFYAIGAYSAGLLASPQFAAVIESFVNTYPSVGNFLVWL 111 MLAL + V + GLL L AF IGAY+A LL F +P Sbjct: 23 MLALSIYVTLS-CGLLSLANAAFMGIGAYTASLLTM--------QFEMPFP--------- 64 Query: 112 CGPEIVQNGIHLSLWLIVPISALLAALFGALLGAPTLKLRGDYLAIVTLGFGEIIRIFMN 171 + + +L AL ++G PTL+L G YLA+ TLGFGE++R+ + Sbjct: 65 ---------------IALAAGGVLPALVALVIGIPTLRLSGVYLAMATLGFGEVVRVIVL 109 Query: 172 NLNAPVNITNGPQGINLIDPIKVFGVSLAGEPGSGSMVQVFGMSMPSVNAYYFLFLLLCI 231 NL+ IT GP G+N +P + ++ + LLL + Sbjct: 110 NLD----ITGGPMGLN---------------------------GVPPITEWWHIVLLLGV 138 Query: 232 GVIFFSVRLQDSRLGRAWVAIREDEIAAKAMGINTRNVKLLAFAMGASFGGVAGAMFGAF 291 I+ RL+ S++GRA+ AI+EDEIAA+ MG+N KLLAF +GA+ GVAG + F Sbjct: 139 -TIYVLARLRRSKIGRAFEAIKEDEIAARLMGVNVAGYKLLAFVIGAAIAGVAGGLNAHF 197 Query: 292 QGFVSPESFSLTESIAVLAMVVLGGIGHIPGVVLGGVILAALPEVLRHVVEPVQMAIFG 350 + P ++ ++ +L M V GG ++ G ++G IL+ LPEVLRH + ++AI G Sbjct: 198 TFTIGPNNYGFENAVDILTMAVFGGTSNLIGPMIGSTILSLLPEVLRH-FKDFRLAING 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.143 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,163,648 Number of extensions: 46078 Number of successful extensions: 308 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 282 Number of HSP's successfully gapped: 30 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)