BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0261.p (422 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 833 0.0 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 401 e-113 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 395 e-111 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 303 2e-83 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 293 2e-80 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 833 bits (2153), Expect = 0.0, Method: Compositional matrix adjust. Identities = 421/422 (99%), Positives = 422/422 (100%) Query: 1 VSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVG 60 VSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVG Sbjct: 12 VSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVG 71 Query: 61 FFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARL 120 FFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARL Sbjct: 72 FFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARL 131 Query: 121 LQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAAL 180 LQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAAL Sbjct: 132 LQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAAL 191 Query: 181 ASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSM 240 ASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSM Sbjct: 192 ASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSM 251 Query: 241 VILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPL 300 VILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPL Sbjct: 252 VILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPL 311 Query: 301 LSGSAALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLS 360 LSGSAALIL+LAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLS Sbjct: 312 LSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLS 371 Query: 361 TGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVREPQYLQ 420 TGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVREPQYLQ Sbjct: 372 TGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVREPQYLQ 431 Query: 421 PH 422 PH Sbjct: 432 PH 433 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 401 bits (1030), Expect = e-113, Method: Compositional matrix adjust. Identities = 203/412 (49%), Positives = 278/412 (67%), Gaps = 3/412 (0%) Query: 7 QRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFMRPV 66 ++R ++A ++GN LEW+DF VY FF+ IIA+LFFP N+ TSLL+A+ATFG+GFFMRPV Sbjct: 6 NKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFFMRPV 65 Query: 67 GGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQGFSA 126 GG+LLG+YADR GRKAA+ V I+LM + +I PTY +IG+ AP++IVVAR+LQGF+ Sbjct: 66 GGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQGFAT 125 Query: 127 GGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASWGWR 186 GGE S+TAFL E AP +R Y SW +AV GA +G VT +L AL SWGWR Sbjct: 126 GGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDSWGWR 185 Query: 187 LPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTD-SPLK--EVIRNFPRETSASFSMVIL 243 LPF+LG++IGPVG +IR H+ ET FLE +K + +P+K +V++ R S VI Sbjct: 186 LPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMGQVIN 245 Query: 244 WTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLLSG 303 TV YV+L MPT++ K L LP F+ M+ + + P G L+DR+GRR +L Sbjct: 246 GTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRTVLLY 305 Query: 304 SAALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLSTGL 363 S+ L++ YP+F ++++APSL LL+ Q++ +I A GP+ A SE FP +V STGL Sbjct: 306 SSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVRSTGL 365 Query: 364 SVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVRE 415 +VAYNLA +FGGFA F +TWL +GS +APA+YV+ AA I +R+ Sbjct: 366 AVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMRD 417 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 395 bits (1015), Expect = e-111, Method: Compositional matrix adjust. Identities = 200/413 (48%), Positives = 276/413 (66%), Gaps = 2/413 (0%) Query: 2 SVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGF 61 +++ Q RRAI+++ IGN LEWFD +Y FAV+IAK FFPTG+D SLLL ATFGV F Sbjct: 11 DITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSF 70 Query: 62 FMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLL 121 FMRP+G ++LG Y+DR GRKAAL ++I+LM VGT MI P+Y SIGL AP I + +++ Sbjct: 71 FMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMI 130 Query: 122 QGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALA 181 QGFSAGGE GS+TAFL E+AP R R ++SSW AS G+++LL A G + L LA Sbjct: 131 QGFSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLA 189 Query: 182 SWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMV 241 SWGWR+PF+ G++I P G YIR HL+E P F E +KT++PL++ + V Sbjct: 190 SWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMRLLIGAGSV 249 Query: 242 ILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLL 301 I+ TV Y+ L Y+PTY+VK L LP + F A V GL + + +P+VG L+D+IGR P + Sbjct: 250 IMATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFM 308 Query: 302 SGSAALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLST 361 S+AL +VL YPMF ++ ++P LL+ Q V G L+ Y + A +++FP T Sbjct: 309 ITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGT 368 Query: 362 GLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVR 414 G+S+AYN+AVTIFGGFA ITWLI TG ++ +++V+ A +S + + R Sbjct: 369 GMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 303 bits (775), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 163/411 (39%), Positives = 241/411 (58%), Gaps = 3/411 (0%) Query: 2 SVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGF 61 S ++Q + ++ GN +E FDF ++ F+A I+K FFP+ N+ SL+L TFG GF Sbjct: 4 STASQSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGF 63 Query: 62 FMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLL 121 MRP+G I+LG Y DRVGR+ L VT+ LMA+GT +I P+Y +IG APL++++ RLL Sbjct: 64 LMRPLGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLL 123 Query: 122 QGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALA 181 QGFSAG E+G + +L E A + +Y SW AS VA+++ AA+G ++ SL A ++ Sbjct: 124 QGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVS 183 Query: 182 SWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDK--TDSPLKEVIRNFPRETSASFS 239 WGWR+PF +G +I PV + IR L+ET F+ K + T + +I N+ + A Sbjct: 184 GWGWRIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENW-KLVIAGMM 242 Query: 240 MVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRP 299 +V + TV Y++ Y PT+ VLKL I + GL + PI+G L+DR+GRRP Sbjct: 243 LVSMTTVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRP 302 Query: 300 LLSGSAALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVL 359 LL L ++ AYP ++ S + +LI +L L A+Y G ++ A +E+ PA V Sbjct: 303 LLVTFTVLTILTAYPAVNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVR 362 Query: 360 STGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT 410 + G S+AY+LA +FGGF T LI T AP ++ AA + T Sbjct: 363 TAGFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIAT 413 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 293 bits (749), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 162/409 (39%), Positives = 234/409 (57%), Gaps = 3/409 (0%) Query: 4 SNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFM 63 + + ++ GN LE FDF +Y F+A I+K FFP+ N+ SL+L TFG GF M Sbjct: 8 AKHSKLSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGTFGAGFLM 67 Query: 64 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 123 RP+G I+LG Y DRVGR+ L VT+ +MA+GT +I P Y +IG+ AP++++ RLLQG Sbjct: 68 RPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVLTGRLLQG 127 Query: 124 FSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASW 183 FSAG E+G + +L E A + +Y SW AS A++ A +G V+ SL ++ W Sbjct: 128 FSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLSPVQISDW 187 Query: 184 GWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTD--SPLKEVIRNFPRETSASFSMV 241 GWR+PF +G +I P + IR L+ET FL K + + +N+ + A MV Sbjct: 188 GWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNW-KIVVAGMMMV 246 Query: 242 ILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLL 301 ++ TV Y++ Y PT+ VLKL + I GL PI+G L+DR+GRRP+L Sbjct: 247 VMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWLPIMGALSDRVGRRPVL 306 Query: 302 SGSAALILVLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLST 361 L L+ AYP+ +++ S S LLI +L L A+Y G + A +E+ PA V + Sbjct: 307 VAFTLLTLLTAYPVMSWLVSDISFKNLLITELWLSFLYASYNGAAVVALTEVMPAHVRTV 366 Query: 362 GLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGT 410 G S+AY+LA IFGGF TWLI +G AP +++ AA + T Sbjct: 367 GFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLLAT 415 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,339,998 Number of extensions: 59630 Number of successful extensions: 460 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 421 Number of HSP's successfully gapped: 22 Length of query: 422 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 334 Effective length of database: 1,203,190 Effective search space: 401865460 Effective search space used: 401865460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)