BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0257.p
         (265 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309579|ref|YP_003773671.1| metal-dependent hydrolase [Herb...   546   e-157
gi|300311792|ref|YP_003775884.1| metal-dependent hydrolase [Herb...    75   6e-15

>gi|300309579|ref|YP_003773671.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 265

 Score =  546 bits (1406), Expect = e-157,   Method: Compositional matrix adjust.
 Identities = 265/265 (100%), Positives = 265/265 (100%)

Query: 1   MKLRVATYNIHKGVTSFAGRPRILALKQALAQLDPDILFLQEVQGRHDLNALRHASNWPQ 60
           MKLRVATYNIHKGVTSFAGRPRILALKQALAQLDPDILFLQEVQGRHDLNALRHASNWPQ
Sbjct: 1   MKLRVATYNIHKGVTSFAGRPRILALKQALAQLDPDILFLQEVQGRHDLNALRHASNWPQ 60

Query: 61  QGQHEYLAGDTHHAAYGMNAVYDHGHHGNALISRFEIASIRNQDVSDHAYESRGILHCVV 120
           QGQHEYLAGDTHHAAYGMNAVYDHGHHGNALISRFEIASIRNQDVSDHAYESRGILHCVV
Sbjct: 61  QGQHEYLAGDTHHAAYGMNAVYDHGHHGNALISRFEIASIRNQDVSDHAYESRGILHCVV 120

Query: 121 PVEGVEVHCYVIHLGLFAGGRRRQIDALIQAVSTSSPPDAPLLIAGDFNDWTNQLSRTLY 180
           PVEGVEVHCYVIHLGLFAGGRRRQIDALIQAVSTSSPPDAPLLIAGDFNDWTNQLSRTLY
Sbjct: 121 PVEGVEVHCYVIHLGLFAGGRRRQIDALIQAVSTSSPPDAPLLIAGDFNDWTNQLSRTLY 180

Query: 181 QNLGVTEVFDQPQPNARGVMQGLRRLGGLSAPIRPARTFPAMMPWLRLDRIYVRGFRVET 240
           QNLGVTEVFDQPQPNARGVMQGLRRLGGLSAPIRPARTFPAMMPWLRLDRIYVRGFRVET
Sbjct: 181 QNLGVTEVFDQPQPNARGVMQGLRRLGGLSAPIRPARTFPAMMPWLRLDRIYVRGFRVET 240

Query: 241 AEVLKDGPWARLSDHAPIVTELHLA 265
           AEVLKDGPWARLSDHAPIVTELHLA
Sbjct: 241 AEVLKDGPWARLSDHAPIVTELHLA 265


>gi|300311792|ref|YP_003775884.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 260

 Score = 75.1 bits (183), Expect = 6e-15,   Method: Compositional matrix adjust.
 Identities = 80/265 (30%), Positives = 112/265 (42%), Gaps = 47/265 (17%)

Query: 3   LRVATYNIHKGVTSFAGRPRILALKQALAQLDPDILFLQEV----QGRHDLNALRHASNW 58
           L VATYNIH G     GR     +   L +L  D++ LQEV      + D+ AL  A   
Sbjct: 36  LTVATYNIH-GAVGTDGRFAPERVAAVLDELQADVVALQEVPLGGADQVDVLALLRAHT- 93

Query: 59  PQQGQHEYLAGDTHHAAYGMNAVYDHGHHGNALISRFEIASIRNQDVSDHAYESRGILHC 118
                          AA G         +GNA++SR+ I +  + D+S  + E RG L  
Sbjct: 94  ------------GMFAAEGPTMESPARRYGNAVLSRYPILATESIDLSFGSREPRGALDA 141

Query: 119 VVPVEGVEVHCYVIHLGLFAGGRRRQIDALIQAVSTSSPPDAPLLIAGDFNDWTNQLSRT 178
            +   G  +     HLGL    RR QI  L+QA  T+   DAP+++ GD N+W     RT
Sbjct: 142 DIDCRGQLLRVVATHLGLKPAERRAQIKRLLQAFDTN---DAPVILLGDVNEWF-MWGRT 197

Query: 179 LYQNLGVTEVFDQPQPNARGVMQGLRRLGGLSAPIRPARTFPAMMPWLRLDRIYVRGFRV 238
           L   +G  E                      +AP  P  TFP+  P   LDRI++   R 
Sbjct: 198 LRWLVGHFE----------------------AAPAPP--TFPSRWPVFALDRIWISPRRR 233

Query: 239 ETAEVLKDGPWARL-SDHAPIVTEL 262
                +     AR+ SDH P++  +
Sbjct: 234 LQHLAVHRSALARVASDHLPLLAHI 258


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.138    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 914,676
Number of extensions: 40010
Number of successful extensions: 122
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 119
Number of HSP's successfully gapped: 2
Length of query: 265
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 181
Effective length of database: 1,222,130
Effective search space: 221205530
Effective search space used: 221205530
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 129 (54.3 bits)