BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0254.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309582|ref|YP_003773674.1| amino acid ABC transporter/sign... 540 e-155 gi|300313886|ref|YP_003777978.1| amino acid ABC transporter peri... 247 1e-66 gi|300313169|ref|YP_003777261.1| amino acid transport periplasmi... 221 7e-59 gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/sign... 185 5e-48 gi|300311590|ref|YP_003775682.1| amino acid ABC transporter/sign... 167 7e-43 >gi|300309582|ref|YP_003773674.1| amino acid ABC transporter/signal transduction system periplasmic binding protein [Herbaspirillum seropedicae SmR1] Length = 269 Score = 540 bits (1392), Expect = e-155, Method: Compositional matrix adjust. Identities = 268/269 (99%), Positives = 268/269 (99%) Query: 46 MHSPSSPARRVQMALLGLLLLGAGAAQADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRL 105 MHSPSSPARRVQMALLGLLLLGAGAAQADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRL Sbjct: 1 MHSPSSPARRVQMALLGLLLLGAGAAQADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRL 60 Query: 106 IGSDVETAQRMAKDFGLELEVVPTTLTNRVPYLQTNKADIVISAFSITPERAKVIDFSVP 165 IGSDVETAQRMAKDFGLELEVVPTTLTNRVPYLQTNKADIVISAFSITPERAKVIDFSVP Sbjct: 61 IGSDVETAQRMAKDFGLELEVVPTTLTNRVPYLQTNKADIVISAFSITPERAKVIDFSVP 120 Query: 166 YAPILVVVGAPRSMKITSPADLAGKKIIATRGTTNDQELTKIAPSSATITRFDDDATSMT 225 YAPILVVVGAPRSMKITSPADLAGKKIIATRGTTNDQELTKIAPSSATITRFDDDATSMT Sbjct: 121 YAPILVVVGAPRSMKITSPADLAGKKIIATRGTTNDQELTKIAPSSATITRFDDDATSMT 180 Query: 226 AMVSGQADIWATSPMLLKVVNQKNPSRDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIR 285 AMVSGQADIWATSPMLLKVVNQKNPSRDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIR Sbjct: 181 AMVSGQADIWATSPMLLKVVNQKNPSRDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIR 240 Query: 286 TNLRNGELNTIYKKYHEAELPPEILHPAS 314 TNL NGELNTIYKKYHEAELPPEILHPAS Sbjct: 241 TNLSNGELNTIYKKYHEAELPPEILHPAS 269 >gi|300313886|ref|YP_003777978.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 247 bits (630), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 124/251 (49%), Positives = 174/251 (69%), Gaps = 1/251 (0%) Query: 63 LLLLGAGAAQADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRLIGSDVETAQRMAKDFGL 122 +L L A AA ADQLD I A K+R+A + P + D+KL+L GSDVE A+ +A+D G+ Sbjct: 18 VLGLTAHAALADQLDDIKKAGKIRIATTSSTPLFGYSDEKLQLAGSDVEAARLLAQDLGV 77 Query: 123 ELEVVPTTLTNRVPYLQTNKADIVISAFSITPERAKVIDFSVPYAPILVVVGAPRSMKIT 182 +L+++P RVP LQ N+ADIV+ + SITPER KVIDFS PYA I +++GAP+SM I Sbjct: 78 QLDLIPVANAARVPTLQANRADIVVGSLSITPERLKVIDFSTPYAVISLIIGAPKSMVIK 137 Query: 183 SPADLAGKKIIATRGTTNDQELTKIAPSSATITRFDDDATSMTAMVSGQADIWATSPMLL 242 ADL GK+I TR T ND LT A I R++DDAT +T+MV+GQ D+++++P L Sbjct: 138 DYADLNGKRIGVTRATPND-TLTTQNAKGAEIMRYEDDATLITSMVTGQVDVFSSTPSNL 196 Query: 243 KVVNQKNPSRDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIRTNLRNGELNTIYKKYHE 302 + + +K P +++E K KA L + + K +PRLKE IDGW++TN++NG+LN IYKKYH Sbjct: 197 QEMQKKAPGKNLEMKFEQKAFDLGIAINKNQPRLKEWIDGWVKTNMKNGKLNAIYKKYHG 256 Query: 303 AELPPEILHPA 313 +LP + +PA Sbjct: 257 RDLPAAVQNPA 267 >gi|300313169|ref|YP_003777261.1| amino acid transport periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 221 bits (562), Expect = 7e-59, Method: Compositional matrix adjust. Identities = 117/253 (46%), Positives = 157/253 (62%), Gaps = 1/253 (0%) Query: 54 RRVQMALLGLLLLGAGAAQADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRLIGSDVETA 113 R+ +LGL A D I A KK+ VAIDL PP+ M + KL GSDVETA Sbjct: 9 RKAAAVVLGLACFAQAAHADALAD-IKARKKVLVAIDLGAPPFGMTNAKLEPQGSDVETA 67 Query: 114 QRMAKDFGLELEVVPTTLTNRVPYLQTNKADIVISAFSITPERAKVIDFSVPYAPILVVV 173 + +AKD G+ELE+V T NR+P+L T KAD+VIS+FSITPER KV+ F+ PY VV Sbjct: 68 RLLAKDLGVELEIVQVTGPNRIPFLMTGKADMVISSFSITPERQKVVAFTQPYGASQFVV 127 Query: 174 GAPRSMKITSPADLAGKKIIATRGTTNDQELTKIAPSSATITRFDDDATSMTAMVSGQAD 233 AP+ I S DL GK++ RG D LT IAP I RFDDDAT A++SGQ D Sbjct: 128 AAPKGENIKSLNDLVGKRVAVVRGNMQDSLLTPIAPKGTNIVRFDDDATVTAAIISGQVD 187 Query: 234 IWATSPMLLKVVNQKNPSRDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIRTNLRNGEL 293 T L + ++NPS+++E+K +K A+GLRKGEP L++ ++ WI NL+NG+L Sbjct: 188 ALCTPNSLASAIAKQNPSKNLETKFAIKDIPYAIGLRKGEPALEKWLNEWISANLKNGKL 247 Query: 294 NTIYKKYHEAELP 306 IY+++ + +P Sbjct: 248 PGIYQQWVGSPMP 260 >gi|300313535|ref|YP_003777627.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 260 Score = 185 bits (469), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 95/247 (38%), Positives = 149/247 (60%) Query: 60 LLGLLLLGAGAAQADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRLIGSDVETAQRMAKD 119 + G ++ A A+AD LD I LRVA+ PP+ D L+ G D++ A +AK Sbjct: 11 MAGAMMFSASVARADALDDIQKHGTLRVAVPADFPPFGSVDADLKPQGLDIDVATLIAKK 70 Query: 120 FGLELEVVPTTLTNRVPYLQTNKADIVISAFSITPERAKVIDFSVPYAPILVVVGAPRSM 179 G+++E++P + NR+PYL T K D+VIS+ ER KVIDFS YAP V P + Sbjct: 71 LGVKVELIPVSSANRIPYLTTKKVDLVISSMGKNAEREKVIDFSAAYAPFFNGVFGPADL 130 Query: 180 KITSPADLAGKKIIATRGTTNDQELTKIAPSSATITRFDDDATSMTAMVSGQADIWATSP 239 + S ADLAGK I TRG+ D ELTKI P+SAT+ R++D+ +++++ +SGQ + AT Sbjct: 131 SVKSAADLAGKTIAVTRGSVEDLELTKIVPASATVKRYEDNNSTISSFLSGQVQLVATGN 190 Query: 240 MLLKVVNQKNPSRDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIRTNLRNGELNTIYKK 299 ++ + +NP + E+K +K + +GL KGE +L++K+D + + ++G L+ I +K Sbjct: 191 VVAAAIIARNPPKKPETKFLIKDSPCFIGLNKGEKKLQDKVDAILAESKKDGSLSAISQK 250 Query: 300 YHEAELP 306 + LP Sbjct: 251 WLGMPLP 257 >gi|300311590|ref|YP_003775682.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 167 bits (424), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 96/259 (37%), Positives = 146/259 (56%) Query: 51 SPARRVQMALLGLLLLGAGAAQADQLDTILAAKKLRVAIDLAVPPYSMKDDKLRLIGSDV 110 SP + + +LGL + G A+AD L I +R+ + P+ + L+L G D+ Sbjct: 4 SPRKTMLAVVLGLAAMQGGLARADALADIQKNGVVRIGVPQDFAPFGSVNTDLQLQGLDI 63 Query: 111 ETAQRMAKDFGLELEVVPTTLTNRVPYLQTNKADIVISAFSITPERAKVIDFSVPYAPIL 170 + A+ +A+ G++L++VP NR+ YLQT+KAD+VIS ER KVIDFS PYAP Sbjct: 64 DVARLIAQKMGVKLQLVPVASANRIAYLQTHKADLVISTLGRNAEREKVIDFSQPYAPYN 123 Query: 171 VVVGAPRSMKITSPADLAGKKIIATRGTTNDQELTKIAPSSATITRFDDDATSMTAMVSG 230 V S+K++ PADLA K + RGT D +LT AP SATI R++D+ ++A VSG Sbjct: 124 NSVFGVASVKVSGPADLADKTVGVARGTFQDLQLTDTAPKSATIKRYEDNNAMISAYVSG 183 Query: 231 QADIWATSPMLLKVVNQKNPSRDIESKLTLKANLLAVGLRKGEPRLKEKIDGWIRTNLRN 290 Q + T + + +K P+ K ++ + VGL KGEP L K++ + ++ Sbjct: 184 QVQLVGTGDFVAYALAEKAPNNKPVLKYIIQESACNVGLNKGEPALLAKVNEALTAAKKS 243 Query: 291 GELNTIYKKYHEAELPPEI 309 GELN I +K+ LP ++ Sbjct: 244 GELNGIVQKWLNVPLPAKM 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 871,550 Number of extensions: 31354 Number of successful extensions: 123 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 113 Number of HSP's successfully gapped: 16 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)