BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0251.p
         (383 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate dehydro...   759   0.0  
gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase [Herbas...   255   4e-69

>gi|300309586|ref|YP_003773678.1| FMN-dependent L-lactate
           dehydrogenase [Herbaspirillum seropedicae SmR1]
          Length = 413

 Score =  759 bits (1961), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 383/383 (100%), Positives = 383/383 (100%)

Query: 1   VQRAQSIEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLPRTLVDVSVRR 60
           VQRAQSIEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLPRTLVDVSVRR
Sbjct: 31  VQRAQSIEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLPRTLVDVSVRR 90

Query: 61  QGRRLFGQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVSTTSLEEVVRR 120
           QGRRLFGQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVSTTSLEEVVRR
Sbjct: 91  QGRRLFGQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVSTTSLEEVVRR 150

Query: 121 SGAQVWQQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDARNFSSPRRLDW 180
           SGAQVWQQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDARNFSSPRRLDW
Sbjct: 151 SGAQVWQQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDARNFSSPRRLDW 210

Query: 181 RNKLDVLRHPRWLIDILYPHGFPRFANLGDLLPPDQTSVRGAAAAILGQSLSAALDWADV 240
           RNKLDVLRHPRWLIDILYPHGFPRFANLGDLLPPDQTSVRGAAAAILGQSLSAALDWADV
Sbjct: 211 RNKLDVLRHPRWLIDILYPHGFPRFANLGDLLPPDQTSVRGAAAAILGQSLSAALDWADV 270

Query: 241 QWLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTMDVLPEVVAAV 300
           QWLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTMDVLPEVVAAV
Sbjct: 271 QWLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTMDVLPEVVAAV 330

Query: 301 KGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRALEILRSEVDR 360
           KGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRALEILRSEVDR
Sbjct: 331 KGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRALEILRSEVDR 390

Query: 361 VLALLACPDIDQLDASYLRRLQA 383
           VLALLACPDIDQLDASYLRRLQA
Sbjct: 391 VLALLACPDIDQLDASYLRRLQA 413


>gi|300311906|ref|YP_003775998.1| L-lactate dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 380

 Score =  255 bits (651), Expect = 4e-69,   Method: Compositional matrix adjust.
 Identities = 145/382 (37%), Positives = 209/382 (54%), Gaps = 18/382 (4%)

Query: 6   SIEELRAMARKRVPNFCFEYVEGGAEEEISLRHNREVFTRIGFLPRTLVDVSVRRQGRRL 65
            +E+ R +A+KRVP   ++Y + G+  E + R N +    +    R  ++V  R     +
Sbjct: 6   CVEDFRLLAKKRVPKAFYDYADSGSYTESTYRANSKDLAALKLRQRVAINVDERSTRTTM 65

Query: 66  FGQDIASPFLIGPTGFSGLLAREGDVAMASAAASAGVPFVLTNVSTTSLEEVVRRSGAQV 125
            G D+  P  I PTG +G+    G++  A AA   G+PF L+ +S  S+E+V   +    
Sbjct: 66  IGHDVTMPVAIAPTGLTGMQWANGEMLGAIAAEKFGIPFTLSTMSICSIEDVASVTTKPF 125

Query: 126 WQQVYLYRDRAFVASVAQRAQAAGIGVLVLTTDSAVYGKREWDARN-FSSPRRLDWRNKL 184
           W Q+Y+ RDR FV S+ +RA+AA    LVLT D  + G+R  D +N  S P +L     L
Sbjct: 126 WFQLYVMRDRGFVKSLIERAKAAKCSALVLTLDLQILGQRHKDLKNGMSVPPKLTLETLL 185

Query: 185 DVLRHPRWLIDILYPHGFPRFANLGDLLPPDQTSVRGAAAAILGQSLSA--------ALD 236
           D+   P W +  L   G   F NL          ++G   A   Q+LS          L+
Sbjct: 186 DLASKPGWALRAL--GGRKTFGNLAG-------HIKGGEGAGGVQTLSKWTASQFDPTLN 236

Query: 237 WADVQWLRGIWPGKLVLKGVMQVEDAQRAVALGVDGIVLSNHGGRQLDGALSTMDVLPEV 296
           W DV W++  W GKL+LKG++ VEDA+ AV  G D IV+SNHGGRQLDGA+S+++ LP +
Sbjct: 237 WDDVAWIKQQWGGKLILKGILDVEDAKLAVQSGADAIVVSNHGGRQLDGAMSSIEALPAI 296

Query: 297 VAAVKGQLTVMLDGGFRRGADIVKAIALGADAVLLGRATTYGLAAGGQAGATRALEILRS 356
             AV  Q+ V  DGG R G D++KA+ALGA   ++GRA  Y L A G  G ++ LEI+R 
Sbjct: 297 AQAVGDQIEVWFDGGIRSGQDVLKAVALGARGTMIGRAFLYSLGAMGGEGVSQMLEIMRK 356

Query: 357 EVDRVLALLACPDIDQLDASYL 378
           E+D  +AL    DI  +    L
Sbjct: 357 ELDVSMALTGTKDIKDVGPQIL 378


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.137    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,237,147
Number of extensions: 54292
Number of successful extensions: 246
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 244
Number of HSP's successfully gapped: 2
Length of query: 383
Length of database: 1,619,870
Length adjustment: 87
Effective length of query: 296
Effective length of database: 1,207,925
Effective search space: 357545800
Effective search space used: 357545800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 131 (55.1 bits)