BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0245.p (596 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 1235 0.0 gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 523 e-149 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 466 e-132 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 134 2e-32 gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbasp... 105 1e-23 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 1235 bits (3196), Expect = 0.0, Method: Compositional matrix adjust. Identities = 593/596 (99%), Positives = 593/596 (99%) Query: 1 MPQYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS 60 MPQYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS Sbjct: 1 MPQYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHS 60 Query: 61 GDREGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLN 120 GDREGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLN Sbjct: 61 GDREGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLN 120 Query: 121 SSNQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR 180 SSNQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR Sbjct: 121 SSNQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLR 180 Query: 181 SKAEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPN 240 SKAEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPN Sbjct: 181 SKAEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPN 240 Query: 241 ADGSLGGRNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG 300 ADGSLG RNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG Sbjct: 241 ADGSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLG 300 Query: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAE 360 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAE Sbjct: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAE 360 Query: 361 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGG 420 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGG Sbjct: 361 GGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGG 420 Query: 421 HGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEH 480 HGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEH Sbjct: 421 HGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEH 480 Query: 481 GTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFAR 540 GTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFAR Sbjct: 481 GTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFAR 540 Query: 541 MAKIALEKQDSGDSFYTAKLATARFYFARLLPETAMLIRQARSGAGNLMALDAELF 596 MAKIALEKQDSGDSFY AKLATARFYFARLLPETAMLIRQARSGA NLMALDAELF Sbjct: 541 MAKIALEKQDSGDSFYAAKLATARFYFARLLPETAMLIRQARSGAANLMALDAELF 596 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 523 bits (1347), Expect = e-149, Method: Compositional matrix adjust. Identities = 272/581 (46%), Positives = 363/581 (62%), Gaps = 27/581 (4%) Query: 3 QYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGD 62 Y PLRD FVL E+L +V + LP HA+ + +L+ QVLEE G+FA+ V+ PLN GD Sbjct: 4 HYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIGD 63 Query: 63 REGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSS 122 REGC + ++ TVTTP GF++AY Q+ GW AL+C PE GGQGLP ++ + YEML+++ Sbjct: 64 REGCRF--KNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAA 121 Query: 123 NQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSK 182 N WTMYPGL HGAY CL AHGS + YL K+ SGEW TMCLTEA G+DLG LR++ Sbjct: 122 NHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTR 181 Query: 183 AEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNAD 242 A PQ DGSY+++G+KIFIS GE+D++ NI+HLVLARLPDAP GSKG+SLFL P+ L + Sbjct: 182 AVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDD-- 239 Query: 243 GSLGGRNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLGVG 302 G RN + C IE KMGIHG+ATC ++ + A WL+G+ +GL AMFV MNAARL VG Sbjct: 240 ---GSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVG 296 Query: 303 MQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAEGG 362 QGLGL E AYQ A YA++R Q R+ D I+ HP V+++L+ + EG Sbjct: 297 AQGLGLAEAAYQRASAYARERRQSRA-----PGSSGNDLIVHHPAVQKLLMEQRCRIEGA 351 Query: 363 RAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGGHG 422 R T + L LD P+ +R + + +TPV+KAFLTD + S LQV+GGHG Sbjct: 352 RLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHG 411 Query: 423 YIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEHGT 482 Y+ E G+EQ++RDARI MIYEGTN IQ++D L RK+L D G +L F ++A + E Sbjct: 412 YVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASG 471 Query: 483 DESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPY------LRVVGHLVFSY 536 + +G+ V KL + S A A A+PY LR+ GH+ + Sbjct: 472 LGEQARL------LGEAVDKLRI---FASRIAAAAAQAPALPYYVADEMLRLCGHVALGW 522 Query: 537 LFARMAKIALEKQDSGDSFYTAKLATARFYFARLLPETAML 577 ++ R + AL +Y K A ++FA + E L Sbjct: 523 MWLRAGRRALSAMAQDPLWYGGKRDAACYHFAFVFAEVQQL 563 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 466 bits (1199), Expect = e-132, Method: Compositional matrix adjust. Identities = 258/600 (43%), Positives = 360/600 (60%), Gaps = 15/600 (2%) Query: 4 YNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGDR 63 Y AP++DM FV++EL + V LP D E + +LEE KF S+V+ PLNHS D+ Sbjct: 3 YTAPVKDMLFVMNELAGLAAV-NALPGCEDATPETAEAILEENAKFCSEVVAPLNHSADK 61 Query: 64 EGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSSN 123 E + VTT GFK+A++Q+ EAGW + EFGGQGLP + EMLNS+N Sbjct: 62 EPSSW--ADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 Query: 124 QAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSKA 183 ++ + P L+ G E L G+++QK+ ++ +SG+WTGTM LTE G+DL L+R++A Sbjct: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 Query: 184 EPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNADG 243 P+ DG+YK+ G KIFI+ GE+D++ NI+HLVLAR P+APEG KGISLF+VPKFL NADG Sbjct: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 Query: 244 SLGGRNPIFCGAIEEKMGIHGNATCQMNL---DGATGWLIGQPHKGLQAMFVFMNAARLG 300 SLG RN + C +IE K+GI + T + GA G L+G+ ++GL+ MF+ MNAAR Sbjct: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 Query: 301 VGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVH-PDVRRMLLTAKAFA 359 VG+QGL ++E AYQ A+ YA+DR+Q R L+G P I+H PDVRRML++ +A Sbjct: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAG-----SPGPVAIIHQPDVRRMLMSMRAQI 354 Query: 360 EGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYG 419 E RA + A D DE RK + L P+VK ++T+ + TS +QV+G Sbjct: 355 EAARALSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHG 414 Query: 420 GHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEE 479 G G+I E G QY RDARI IYEGT IQ+ DL+GRK D GA K AQ++ V E Sbjct: 415 GMGFIEETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSE 474 Query: 480 HGTDESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAAAVPYLRVVGHLVFSYLFA 539 D + L + D + ++ + ++ A +VPYL++ G ++ + A Sbjct: 475 LQGDAQLQPIAARLGEAADALEQVVDYVVANVKSDIKAVFAGSVPYLKMAGVVLGGWQMA 534 Query: 540 RMAKIALEKQDSGD---SFYTAKLATARFYFARLLPETAMLIRQARSGAGNLMALDAELF 596 R A ++ K +G+ FY AKLATARFY ++LP+ G +MAL E F Sbjct: 535 RAAAVSAAKLKAGEGDGKFYEAKLATARFYADQILPQALAYRTAIVDGGAAVMALAEEQF 594 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 134 bits (338), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 120/418 (28%), Positives = 185/418 (44%), Gaps = 59/418 (14%) Query: 49 FASQVLFPLNHSGDREGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLP 108 FA + L P DR T P +A ++ E G + ++GG G+ Sbjct: 17 FAQERLLPFAADWDRNH----------TFPA---EALKELAELGAMGMCVPEQWGGAGMD 63 Query: 109 -LTINNSFYEMLNSSNQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCL 167 +++ + E+ T+ + A +GS QK+ +L L GE G CL Sbjct: 64 YMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCL 123 Query: 168 TEAHCGTDLGLLRSKAEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSK 227 TE H G+D +R++AE D + + G K FI++G++ A +V A + D G K Sbjct: 124 TEPHTGSDAAAIRTRAERDGD-DFILNGTKQFITSGKHAGVA----IVFA-VTDRSAGKK 177 Query: 228 GISLFLVPKFLPNADGSLGGRNPIFCGAIEEKMGIHGNATCQMNLDGA---TGWLIGQPH 284 GIS FLVP P G + GR+ EEKMG H + T Q+ LD L+G+ Sbjct: 178 GISCFLVPCDTP---GFVVGRS-------EEKMGQHASDTVQIMLDNCRVPATALLGKEG 227 Query: 285 KGLQAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIV 344 +G + + A R+G+ Q +G+ A++ A+ YA+ R G+ PII Sbjct: 228 EGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESF----GV--------PIIE 275 Query: 345 HPDVRRMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLT 404 H V L + R + A +L KD + K F + Sbjct: 276 HQAVNFRLADMNTLLDAARLMV-WRAAQL------------KDQGRPCLKEASMAKMFAS 322 Query: 405 DNAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDN 462 + A S+ +Q++GG GY +++ +E+ RD RI IYEG N IQ L ++GR I D Sbjct: 323 EAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGANDIQRL-VIGRAIASDQ 379 >gi|300310313|ref|YP_003774405.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 550 Score = 105 bits (261), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 85/299 (28%), Positives = 141/299 (47%), Gaps = 47/299 (15%) Query: 165 MCLTEAHCGTDLGLLRSKA------EPQADG---SYKITGNKIFISAGENDVSANIIHLV 215 M +TE G+D+ RS A +P DG +Y ++G+K F SA D HL+ Sbjct: 178 MGMTEKQGGSDV---RSNASIAMPVDPLNDGRGAAYLLSGHKWFFSAPMCDA-----HLM 229 Query: 216 LARLPDAPEGSKGISLFLVPKFLPNADGSLGGRNPIFCGAIEEKMGIHGNATCQMNLDGA 275 LAR + G+S F VP++LP+ G RNPI +++K+G N++ ++ + A Sbjct: 230 LARTEN------GLSCFFVPRWLPD-----GTRNPILIQRLKDKLGNRSNSSSEVEFEEA 278 Query: 276 TGWLIGQPHKGLQAMFVFMNAARLGVGMQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAA 335 G ++G +G+ + N RL + L + ALV A + RS G + A Sbjct: 279 MGIMVGDEGRGIPTIIEMANHTRLDCIIGSAAL----MRQALVQAIHHARHRSAFGRRLA 334 Query: 336 DKPADPIIVHPDVRRMLLTAKAFAEGGRAFTSYVALELDKELTHPDEQVRKDSADIVALL 395 ++P +R +L +E VA D PD+ +++ I+ Sbjct: 335 EQPL--------MRAVLADLALESEAATMLMLRVAHAFDA----PDDPLQRAWKRII--- 379 Query: 396 TPVVKAFLTDNAWMATSECLQVYGGHGYIAEWGMEQYVRDARINMIYEGTNTIQSLDLL 454 TP K ++ T EC++V+GG+GY+ M + R+A +N I+EG+ + LD+L Sbjct: 380 TPAAKFWICKRTLEFTGECMEVWGGNGYVETAPMARLYREAPVNSIWEGSGNVMCLDVL 438 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,949,149 Number of extensions: 86071 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 209 Number of HSP's successfully gapped: 8 Length of query: 596 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 505 Effective length of database: 1,188,985 Effective search space: 600437425 Effective search space used: 600437425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)