BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0244.p (795 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase... 1618 0.0 gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase... 114 2e-26 gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase... 65 2e-11 >gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 805 Score = 1618 bits (4190), Expect = 0.0, Method: Compositional matrix adjust. Identities = 793/795 (99%), Positives = 794/795 (99%) Query: 1 VSNFIVKKVAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSP 60 VSNFIVKKVAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSP Sbjct: 11 VSNFIVKKVAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSP 70 Query: 61 APLGNKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFAS 120 APLGNKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFAS Sbjct: 71 APLGNKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFAS 130 Query: 121 NTSGLSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTT 180 NTSGLSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTT Sbjct: 131 NTSGLSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTT 190 Query: 181 LGKGVVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTA 240 LGKGVVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTA Sbjct: 191 LGKGVVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTA 250 Query: 241 DVVGLDTMGHVIKTMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVGKDIL 300 DVVGLDTMGHVI+TMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVGKDIL Sbjct: 251 DVVGLDTMGHVIRTMQDNLKDDPFFSSYATPPLLAKLVEQGALGQKSGAGFYKKVGKDIL 310 Query: 301 RIDPAKGDYVPGGAKADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFHYIAV 360 RIDPAKGDYVPGGAKADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFHYIAV Sbjct: 311 RIDPAKGDYVPGGAKADDIIARILKEKDPVKRMKALHDSTNPQGQFLWAIFRDAFHYIAV 370 Query: 361 HLESVADNARDLDFAMRWGFGWSVGPFETWQAAGWKQIAQWVKEDIDAGKALSTAPLPDW 420 HLESVADNARDLDFAMRWGFGWSVGPFETWQAAGWKQIAQWVKEDIDAGKALSTAPLPDW Sbjct: 371 HLESVADNARDLDFAMRWGFGWSVGPFETWQAAGWKQIAQWVKEDIDAGKALSTAPLPDW 430 Query: 421 VFDGRDGVHSEKGSYSPSKKADVARSELPVYQRQAFRAPLVGEGAADGKSAGTTFFEDES 480 VFDGRDGVHSEKGSYSPSKKADVARSELPVYQRQAFRAPLVGEGAADGKSAGTTFFEDES Sbjct: 431 VFDGRDGVHSEKGSYSPSKKADVARSELPVYQRQAFRAPLVGEGAADGKSAGTTFFEDES 490 Query: 481 VRIWHQNDDVLILSFKTKMHVIGQGVIDGMNKAVSEAEQNFKGLVIWHPDAAEGGAFSAG 540 VRIWHQNDDVLILSFKTKMHVIGQGVIDGMNKAVSEAEQNFKGLVIWHPDAAEGGAFSAG Sbjct: 491 VRIWHQNDDVLILSFKTKMHVIGQGVIDGMNKAVSEAEQNFKGLVIWHPDAAEGGAFSAG 550 Query: 541 ADLQSMLPLFMSGGVKAIEPVVQQLQQAHQRLKYASVPVIAAVAGLALGGGCELLLHTAK 600 ADLQSMLPLFMSGGVKAIEPVV QLQQAHQRLKYASVPVIAAVAGLALGGGCELLLHTAK Sbjct: 551 ADLQSMLPLFMSGGVKAIEPVVHQLQQAHQRLKYASVPVIAAVAGLALGGGCELLLHTAK 610 Query: 601 RVVSIESYIGLVEVGVGLIPAGGGLKEAAVRAAREAKGNDILPFLKEYMLAAATANVSKS 660 RVVSIESYIGLVEVGVGLIPAGGGLKEAAVRAAREAKGNDILPFLKEYMLAAATANVSKS Sbjct: 611 RVVSIESYIGLVEVGVGLIPAGGGLKEAAVRAAREAKGNDILPFLKEYMLAAATANVSKS 670 Query: 661 ALEARKLGYLREDDVIVFNPYELLHVAKVEARALFDAGYRPQLPPQVPVIGRNGLATIMA 720 ALEARKLGYLREDDVIVFNPYELLHVAKVEARALFDAGYRPQLPPQVPVIGRNGLATIMA Sbjct: 671 ALEARKLGYLREDDVIVFNPYELLHVAKVEARALFDAGYRPQLPPQVPVIGRNGLATIMA 730 Query: 721 QLVNMRDGGFISAHDFKLGKMIAETVSGGEVEEGSIVNEQWLLDIERKFFMELLNHPKTQ 780 QLVNMRDGGFISAHDFKLGKMIAETVSGGEVEEGSIVNEQWLLDIERKFFMELLNHPKTQ Sbjct: 731 QLVNMRDGGFISAHDFKLGKMIAETVSGGEVEEGSIVNEQWLLDIERKFFMELLNHPKTQ 790 Query: 781 ERIMGMMQTGKPVRN 795 ERIMGMMQTGKPVRN Sbjct: 791 ERIMGMMQTGKPVRN 805 >gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 512 Score = 114 bits (286), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 97/290 (33%), Positives = 140/290 (48%), Gaps = 18/290 (6%) Query: 9 VAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSPAPLGNKDD 68 VAV+G+G MGA IA A PV+L+D ++EG + NL KL +K Sbjct: 16 VAVIGSGTMGAGIAQVAAVAGHPVLLYD--SREGAAGKAIGAICTNLDKLVSK---DKLT 70 Query: 69 AALIQVANYE----DDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASNTSG 124 AL Q A+ L L DL+IEAI E++ KH+L+ K+ + + I A+NTS Sbjct: 71 PALAQAASQRISVAQSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVSTRCILATNTSS 130 Query: 125 LSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTTLGKG 184 LSI ++ G R G+HFFNP M LVE+I +++ L Q GK Sbjct: 131 LSITQIAAGLQH--PERVVGMHFFNPVPMMALVEIISGLASDA-ALAQCLHDTALAWGKT 187 Query: 185 VVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTADVVG 244 V A TP F+ NR+ + + G +D + + G + G F D++G Sbjct: 188 PVMARSTPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVM-REAGGFRMGPFELMDLIG 246 Query: 245 LDTMGHVIKTMQDNLKDDPFFSSYATPPLLAK-LVEQGALGQKSGAGFYK 293 D V +++ +DP F TP L+ + LV G LG+KSG GFY+ Sbjct: 247 HDVNFAVTQSVFHAYFNDPRF----TPSLIQQELVHAGYLGRKSGRGFYR 292 >gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 65.5 bits (158), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 54/195 (27%), Positives = 90/195 (46%), Gaps = 8/195 (4%) Query: 6 VKKVAVLGAGVMGAQIAAHCINARVPVVLFD-LPAKEGPKNGIVQRAIENLKKLSPAPLG 64 + +VAV+G GV+GA AA+ + + V D +P E + R +L+ L G Sbjct: 15 IARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIARHWPSLQAL-----G 69 Query: 65 NKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASNTSG 124 + A+ ++ Y+ A LEG D + E ER D K DL++++ + + + AS++SG Sbjct: 70 LAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQVVLASSSSG 129 Query: 125 LSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTTLGKG 184 L ++ + R H FNPP + LVE+I T P + F K Sbjct: 130 LLMSKVQAACRH--PQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFYAAIGKKP 187 Query: 185 VVRAFDTPNFVANRV 199 + + +ANR+ Sbjct: 188 IHVRKEVKGHIANRL 202 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,511,493 Number of extensions: 110242 Number of successful extensions: 354 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 349 Number of HSP's successfully gapped: 3 Length of query: 795 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 702 Effective length of database: 1,179,515 Effective search space: 828019530 Effective search space used: 828019530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)