BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0240.p (451 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferas... 438 e-124 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 155 9e-39 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 149 7e-37 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 129 6e-31 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 128 1e-30 >gi|300309354|ref|YP_003773446.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 438 bits (1127), Expect = e-124, Method: Compositional matrix adjust. Identities = 220/442 (49%), Positives = 294/442 (66%), Gaps = 4/442 (0%) Query: 1 MTTTTDLTMVNAYIPGR-ADVGPVTEALIERRNALLGPAYRLMYEHPLHIVRGEGVWLID 59 MT + +NA+ P + P T ALIERR G L Y P+H V GVWL D Sbjct: 1 MTHADGMLALNAFNPQSGSTTAPATRALIERRRNTFGDGVPLFYRDPVHFVSASGVWLRD 60 Query: 60 PQGRRYLDVYNNVTSLGHCHPAVTEAICQQVQTLATNTRYLHETILELAERLLATVPDTA 119 G YLD YNNV S+GHCHP V EA+ +Q TL T+TRYL + + AERLL T+P Sbjct: 61 QDGLEYLDAYNNVPSVGHCHPRVVEAVSKQAATLNTHTRYLSDVVTSYAERLLGTLP-RE 119 Query: 120 LAHLMLTCTGSEANDLAYRIAKVRTGGVGVIVTETAYHGITDAVSQFSPSLGSSVNLGPH 179 L+ ++ T +G+E+ DLA R+A++ TGG G IVT AYHG + AV++ +P+ G V +G + Sbjct: 120 LSKVLFTSSGTESVDLAMRLARLYTGGSGFIVTRFAYHGHSTAVAEITPAFGPGVPIGIN 179 Query: 180 VRLVPAPRAYHAEGQDLGERFTRDVQAAIADLQRHGIQPAALIVDSLFTSDGILPGAAGF 239 VRLV AP Y + +G +F +DV AAIAD++RHGI+ A LI+D++ +SDG+ GF Sbjct: 180 VRLVDAPDGYRQDAP-VGIKFAQDVAAAIADMERHGIRFAGLILDTVCSSDGLFVDPPGF 238 Query: 240 LKGAVAAIKEAGGIFIADEVQPGFGRTGEHMWGFQRHGVTPDIVTMGKPMGNGQPVSGLL 299 LK A+A +K+AGG+FIADEVQPGFGRTGE MWGF+RH + PDIV MGKPMGNG P++ + Sbjct: 239 LKDAIATVKKAGGVFIADEVQPGFGRTGEGMWGFERHRIVPDIVVMGKPMGNGMPIAATV 298 Query: 300 ATADVLQEFGQRSRYFNTFAGNTVSCAAALAVLNTIEREGLIAHARQVGAVLLEGIGALA 359 AD++Q F +RS YFNTF GNTV CAAA AVL+ IE EGLI +A++ GA L G+ L Sbjct: 299 TRADIMQAFTERSGYFNTFGGNTVCCAAASAVLDVIESEGLINNAKETGASLKLGLEQLK 358 Query: 360 GRHEAIGEVRGAGMFVGVELVSDRDRRTPDRALTSEVVNRMRQKGVLLSACAMGHNVLKI 419 G+H+ IG+VR G++ VE V D +T D + VVN +RQK +L+S C N+LKI Sbjct: 359 GQHQCIGDVRAIGLYAAVEFVLP-DTKTADTDMALHVVNTLRQKRILISTCGPAGNILKI 417 Query: 420 RPPLVLTTEQAGMVIEALDASL 441 RPPL + + +++ A+D L Sbjct: 418 RPPLQFSRQHCDLLVTAIDDVL 439 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 155 bits (391), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 119/397 (29%), Positives = 193/397 (48%), Gaps = 24/397 (6%) Query: 46 PLHIVRGEGVWLIDPQGRRYLDVYNNV--TSLGHCHPAVTEAICQQVQTLATNTRYLHET 103 P+ + + GV DP G ++D+ V +S+G C+P V EAI +Q ++L + Sbjct: 48 PIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMHSMEVNSSK 107 Query: 104 ILELAERLLATVPDTALAHLM--LTCTGSEANDLAYRIAKVRTGGVGVIVTETAYHGITD 161 ELA ++ +PD + T GS+A + A + AK TG +I YHGI + Sbjct: 108 RTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRVTGRHQIIAFHGGYHGIWN 167 Query: 162 AVSQFSPSLGSSVNLGPHVRLV---PAPRAY-------HAEGQDLGERFTRDVQAAIADL 211 A + + GP + V P P AY H + + + V + Sbjct: 168 ASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGEY---VDYLLNTP 224 Query: 212 QRHGIQPAALIVDSLFTSDGILPGAAGFLKGAVAAIKEAGGIFIADEVQPGFGRTGEHMW 271 AA+IV+ + G +P + FL+ A +G + I DEVQ G GRTG+ MW Sbjct: 225 YTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAGAGRTGK-MW 283 Query: 272 GFQRHGVTPDIVTMGKPMGNGQPVSGLLATADVLQEFGQRSRYFNTFAGNTVSCAAALAV 331 + GV PD++T GK +G P++GL+ +D+ + S+ NTFA N++S A AL Sbjct: 284 AVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQP-NTFAANSISAAVALTN 342 Query: 332 LNTIE--REGLIAHARQVGAVLLEGIGALAGRHEAIGEVRGAGMFVGVELVSDRDRRTP- 388 ++ ++ R L+ A +G E I + +GEVRG G+ +G+ELV +R+ R P Sbjct: 343 ISILQDPRLDLVNRAHTLGLEAQERIRSF--NSPWVGEVRGRGLMIGIELVENRETREPL 400 Query: 389 DRALTSEVVNRMRQKGVLLSACAMGHNVLKIRPPLVL 425 R ++++ + GVL+ C NV+++ P L + Sbjct: 401 SREKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTI 437 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 149 bits (375), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 124/414 (29%), Positives = 194/414 (46%), Gaps = 25/414 (6%) Query: 24 TEALIERRNALLGPAYRLMYEHPLHIVRGEGVWLIDPQGRRYLDVYNNVTSL--GHCHPA 81 + L +R+NA +M + + R L D +GRR++D + L GH HP Sbjct: 7 NQELQQRKNAATPRGVGVMCD--FYAERAANAELWDVEGRRFIDFAAGIAVLNTGHRHPK 64 Query: 82 VTEAICQQVQTLATNTRYL---HETILELAERLLATVPDTALAHLMLTCTGSEANDLAYR 138 + +A+ Q+ T+T Y + + +ELAER+ P TG+EA + A + Sbjct: 65 LLDAMRAQMDKF-THTAYQIVPYASYVELAERINRLTPGNYPKKTAFFSTGAEAVENAIK 123 Query: 139 IAKVRTGGVGVIVTETAYHGITDAVSQFSPSLGS-SVNLGPH---VRLVPAPRAYHAEGQ 194 IA+ TG GVI +HG T + + + GP V P P A H Sbjct: 124 IARAHTGRPGVIAFAGGFHGRTMMGMALTGKVAPYKLGFGPFPGDVFHAPYPSALHG--- 180 Query: 195 DLGERFTRDVQAAIADLQRHGIQP---AALIVDSLFTSDGILPGAAGFLKGAVAAIKEAG 251 + D A+ L + I+ AA+I++ + G A F++G A E G Sbjct: 181 ----ITSEDALEAVKGLFKSDIEAKRVAAIILEPVQGEGGFYAAPADFMRGLRALCDEHG 236 Query: 252 GIFIADEVQPGFGRTGEHMWGFQRHGVTPDIVTMGKPMGNGQPVSGLLATADVLQEFGQR 311 + IADEVQ G+GRTG+ ++ + + V PD++TM K + G P+S + A+++ + Sbjct: 237 ILLIADEVQSGYGRTGK-LFAMEHYDVLPDLMTMAKSLAGGMPLSAVNGRAEIM-DAPAP 294 Query: 312 SRYFNTFAGNTVSCAAALAVLNTIEREGLIAHARQVGAVLLEGIGALAGRHEAIGEVRGA 371 T+AGN ++ A+ALAVL+ +E E L+ +++G L E + L I EVRG Sbjct: 295 GGLGGTYAGNPLAIASALAVLDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGV 354 Query: 372 GMFVGVELVSDRDRRTPDRALTSEVVNRMRQKGVLLSACAMGHNVLKIRPPLVL 425 G V VE +D PD T +V G+LL C NV++ PL + Sbjct: 355 GAMVAVEF-ADPATGKPDAEYTKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTI 407 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 129 bits (323), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 128/449 (28%), Positives = 201/449 (44%), Gaps = 40/449 (8%) Query: 26 ALIERRNALLGPAYRLMYEH----PLHIVRGEGVWLIDPQGRRYLDVYNNV--TSLGHCH 79 A ++RR+ L+ P L EH PL + G+G +L D Q ++ LD ++ + + G+ Sbjct: 7 AALDRRH-LIHPVTSLR-EHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQ 64 Query: 80 PAVTEAICQQVQTLATNTRYLH---ETILELAERLLATVPDTALAHLMLTCTGSEANDLA 136 +V A +Q+Q L T Y H E + LA++L+ P +L H+ T GS+A D A Sbjct: 65 ESVIRAATEQMQRLPYATGYFHFASEPAILLAKKLVDISP-ASLQHVYFTLGGSDAVDAA 123 Query: 137 YRIAKVRTGGVG------VIVTETAYHGITDAVSQFS--PSLGSSVNLG-PHVRLVPAPR 187 R +G I + YHG + + + P+ NL P +P+P Sbjct: 124 IRYITHYHHSIGKPGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPY 183 Query: 188 AYHAEGQDLGERFTRDVQA---AIADLQRHGIQPAALIVDSLFTSDGILPGAAGFLKGAV 244 Y +E Q VQA +A+L + AA + + S G++ G+LK Sbjct: 184 PYRSELQGDEAIIAASVQALRDKVAELGADNV--AAFFCEPIQGSGGVVVPPKGWLKAMQ 241 Query: 245 AAIKEAGGIFIADEVQPGFGRTGEHMWGFQRHGVTPDIVTMGKPMGNGQPVSGLLATADV 304 A E +F+ DEV GFGRTG M+ V PD++TM K + G G + +D Sbjct: 242 LACNELDILFVVDEVITGFGRTGP-MFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDR 300 Query: 305 LQE-------FGQRSRYFNTFAGNTVSCAAALAVLNTIEREGLIAHARQVGAVLLEGIGA 357 + + T++ + VS A AL VL E GL+ + R++ V G+ A Sbjct: 301 VYNGIADGAPMSTVVGHGQTYSAHPVSAAVALEVLRLYEEGGLLDNGRRLEPVFAGGLRA 360 Query: 358 LAGRHEAIGEVRGAGMFVGVELVSDRD---RRTPDRALTSEVVNRMRQKGVLLSACAMGH 414 L H +G+ R G+ +ELV+D+ R P+ L + + G++ A G Sbjct: 361 LLA-HPLVGDARSRGLLGALELVADKQTKARFAPELKLHDRIFQAAYRHGLVFR--AFGD 417 Query: 415 NVLKIRPPLVLTTEQAGMVIEALDASLTE 443 N+L P L T E ++ L A L E Sbjct: 418 NILGFAPALCYTQEDFALMFARLKAILDE 446 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 125/430 (29%), Positives = 202/430 (46%), Gaps = 57/430 (13%) Query: 50 VRGEGVWLIDPQGRRYLDVYNN--VTSLGHCHPAVTEAICQQVQTLATNTRYLHETILEL 107 V GEG+ LID G+RY+D V+ LGH HP V EAI +QV LA Y H + Sbjct: 17 VAGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELA----YAHTSFFTT 72 Query: 108 A--ERLLATVPDTA---LAHLMLTCTGSEANDLAYRIAKVRTGGVG------VIVTETAY 156 A E L A + D A L H+ GSEA + A ++A+ VG +I +Y Sbjct: 73 APAEELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQPQRRHIIARRQSY 132 Query: 157 HGITDAV----------SQFSPSLGSSVNLGPHVRLVPAPRAYHAEGQDLGERFTRDVQA 206 HG T F P L + ++ P Y + GE + VQ Sbjct: 133 HGNTLGALAIGGNAWRREMFMPMLIEAHHVSP---------CYAYRNRADGESDVQYVQR 183 Query: 207 AIADLQRHGI-----QPAALIVDSLF-TSDGILPGAAGFLKGAVAAIKEAGGIFIADEVQ 260 +L++ + Q A + +++ + G +P + + A + G + I DEV Sbjct: 184 LADELEQKILSLGADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVM 243 Query: 261 PGFGRTGEHMWGFQRHGVTPDIVTMGKPMGNG-QPVSGLLATADVLQEFGQRSRYF---N 316 G GRTG +++ + GV PDIV + K +G G QP+ ++ + + + S +F + Sbjct: 244 SGMGRTG-YLFACEEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGH 302 Query: 317 TFAGNTVSCAAALAVLNTIEREGLIAHARQVGAVLLEGIGALAGRHEAIGEVRGAGMFVG 376 T+ G+ +CAAA+AV TI+ E L+ + RQ G L + G +G++RG G+FVG Sbjct: 303 TYIGHATACAAAVAVQKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVG 362 Query: 377 VELVSDRDRR---TPDRALTSEVVNRMRQKGVLLSACA------MGHNVLKIRPPLVLTT 427 VELV++R + +PD + V ++G+L+ G ++L + PP + ++ Sbjct: 363 VELVAERSSKLPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHIL-LAPPFIASS 421 Query: 428 EQAGMVIEAL 437 +++ L Sbjct: 422 NDISEIVQRL 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,513,951 Number of extensions: 70757 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 9 Length of query: 451 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 363 Effective length of database: 1,203,190 Effective search space: 436757970 Effective search space used: 436757970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)