BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0233.p (671 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309604|ref|YP_003773696.1| diguanylate cyclase [Herbaspiri... 1347 0.0 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 254 1e-68 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 240 3e-64 gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodies... 238 2e-63 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 231 1e-61 >gi|300309604|ref|YP_003773696.1| diguanylate cyclase [Herbaspirillum seropedicae SmR1] Length = 696 Score = 1347 bits (3485), Expect = 0.0, Method: Compositional matrix adjust. Identities = 671/671 (100%), Positives = 671/671 (100%) Query: 1 MFRMKMFHTPTPQTGVQAPAAQLSLQQLIRADQLAALRQTVLLSIPVNMVLGAICTFVAW 60 MFRMKMFHTPTPQTGVQAPAAQLSLQQLIRADQLAALRQTVLLSIPVNMVLGAICTFVAW Sbjct: 26 MFRMKMFHTPTPQTGVQAPAAQLSLQQLIRADQLAALRQTVLLSIPVNMVLGAICTFVAW 85 Query: 61 RAGHLAIALLWLACSGMVNLLRILACRLPVQRVAALVQRIAAPGAGVIDLNLRVHWMLAL 120 RAGHLAIALLWLACSGMVNLLRILACRLPVQRVAALVQRIAAPGAGVIDLNLRVHWMLAL Sbjct: 86 RAGHLAIALLWLACSGMVNLLRILACRLPVQRVAALVQRIAAPGAGVIDLNLRVHWMLAL 145 Query: 121 CSGLVWAGVALLCAGYTAPGTLFYLTCVCGITAGAVTHGFAYARIPIAFITPPLLAIIAS 180 CSGLVWAGVALLCAGYTAPGTLFYLTCVCGITAGAVTHGFAYARIPIAFITPPLLAIIAS Sbjct: 146 CSGLVWAGVALLCAGYTAPGTLFYLTCVCGITAGAVTHGFAYARIPIAFITPPLLAIIAS 205 Query: 181 LVLVEPDTTRVALAAAVLLYLGALIRGSKVSEGLVSHASALKNEATTASQALEVAHRDLH 240 LVLVEPDTTRVALAAAVLLYLGALIRGSKVSEGLVSHASALKNEATTASQALEVAHRDLH Sbjct: 206 LVLVEPDTTRVALAAAVLLYLGALIRGSKVSEGLVSHASALKNEATTASQALEVAHRDLH 265 Query: 241 QFAERMRYQAEHDLLTGLLGRAGFMAAAQAMAARREEVLCMMFLDLDGFKVINDAYGHDA 300 QFAERMRYQAEHDLLTGLLGRAGFMAAAQAMAARREEVLCMMFLDLDGFKVINDAYGHDA Sbjct: 266 QFAERMRYQAEHDLLTGLLGRAGFMAAAQAMAARREEVLCMMFLDLDGFKVINDAYGHDA 325 Query: 301 GDQVLVEVARRLQGCLPAGATLARLGGDEFVILYALDAQAELPELLARRLIEVVRQPFAR 360 GDQVLVEVARRLQGCLPAGATLARLGGDEFVILYALDAQAELPELLARRLIEVVRQPFAR Sbjct: 326 GDQVLVEVARRLQGCLPAGATLARLGGDEFVILYALDAQAELPELLARRLIEVVRQPFAR 385 Query: 361 LAHRHIGLSLGITLSDRDDIDEMLVCSDAAMYQAKRRGRNQFCVFNETLNAHLQMKRDVE 420 LAHRHIGLSLGITLSDRDDIDEMLVCSDAAMYQAKRRGRNQFCVFNETLNAHLQMKRDVE Sbjct: 386 LAHRHIGLSLGITLSDRDDIDEMLVCSDAAMYQAKRRGRNQFCVFNETLNAHLQMKRDVE 445 Query: 421 RDLAGALARRELAVWFQPIVQHDGQTLDGFEALLRWQHGKHGAIAAPDLIEIAALAGLSE 480 RDLAGALARRELAVWFQPIVQHDGQTLDGFEALLRWQHGKHGAIAAPDLIEIAALAGLSE Sbjct: 446 RDLAGALARRELAVWFQPIVQHDGQTLDGFEALLRWQHGKHGAIAAPDLIEIAALAGLSE 505 Query: 481 ELLRFILEEVAGMIAFLQSSGRSDLRISMNISPREMERLVVDELVVSMLKAHRLPARMLE 540 ELLRFILEEVAGMIAFLQSSGRSDLRISMNISPREMERLVVDELVVSMLKAHRLPARMLE Sbjct: 506 ELLRFILEEVAGMIAFLQSSGRSDLRISMNISPREMERLVVDELVVSMLKAHRLPARMLE 565 Query: 541 IEITEETALDLQAATQTLVALALQGVRIAIDDFGVGYSSLGFLRQMQVSRVKIDRSFVTG 600 IEITEETALDLQAATQTLVALALQGVRIAIDDFGVGYSSLGFLRQMQVSRVKIDRSFVTG Sbjct: 566 IEITEETALDLQAATQTLVALALQGVRIAIDDFGVGYSSLGFLRQMQVSRVKIDRSFVTG 625 Query: 601 LADQTENQALVSAVLQLAGSFGFQVVAEGVESAEDLQTLQAMHCHAMQGYYFAPPMPAAQ 660 LADQTENQALVSAVLQLAGSFGFQVVAEGVESAEDLQTLQAMHCHAMQGYYFAPPMPAAQ Sbjct: 626 LADQTENQALVSAVLQLAGSFGFQVVAEGVESAEDLQTLQAMHCHAMQGYYFAPPMPAAQ 685 Query: 661 ARARIEALAQA 671 ARARIEALAQA Sbjct: 686 ARARIEALAQA 696 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 254 bits (649), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 166/427 (38%), Positives = 239/427 (55%), Gaps = 8/427 (1%) Query: 237 RDLHQFAERMRYQAEHDLLTGLLGRA---GFMAAAQAMAARREEVLCMMFLDLDGFKVIN 293 R L E + HD LT L R + A AAR + +MF+DLDGFK IN Sbjct: 253 RSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAIN 312 Query: 294 DAYGHDAGDQVLVEVARRLQGCLPAGATLARLGGDEFVILYALDAQAELPELLARRLIEV 353 D+ GH GD++LVEVA+RL A T+ARLGGDEFVIL L+ E +A +L+E Sbjct: 313 DSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNT-PEDAMGVAEKLVEA 371 Query: 354 VRQPFARLAHR-HIGLSLGITLSDRDDID--EMLVCSDAAMYQAKRRGRNQFCVFNETLN 410 V QPF H + S+GI + D ++++ +DAAMY KR GRN + F ++N Sbjct: 372 VNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMN 431 Query: 411 AHLQMKRDVERDLAGALARRELAVWFQPIVQHDGQTLDGFEALLRWQHGKHGAIAAPDLI 470 A+ Q + +DL AL R + + +QP + G EALLRW+H HG +A I Sbjct: 432 ANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFI 491 Query: 471 EIAALAGLSEELLRFILEEVAGMIAFLQSSGRSDLRISMNISPREMERLVVDELVVSMLK 530 +A +GL + ++L+E + + G D +S+N+S + + E+V + L+ Sbjct: 492 GLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLE 551 Query: 531 AHRLPARMLEIEITEETAL-DLQAATQTLVALALQGVRIAIDDFGVGYSSLGFLRQMQVS 589 H+LP L +E+TE TA+ D +A+ QTL +A GV I+IDDFG GYSSL +L+++ + Sbjct: 552 RHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPAN 611 Query: 590 RVKIDRSFVTGLADQTENQALVSAVLQLAGSFGFQVVAEGVESAEDLQTLQAMHCHAMQG 649 +KIDR FV L E+ A+VSA++ L + ++VAEGVE+ E L A C A+QG Sbjct: 612 ELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQG 671 Query: 650 YYFAPPM 656 Y PM Sbjct: 672 YLMGRPM 678 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 240 bits (612), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 157/446 (35%), Positives = 244/446 (54%), Gaps = 11/446 (2%) Query: 234 VAHRDLHQFAERMRYQAEHDLLTGLLGRA---GFMAAAQAMAARREEVLCMMFLDLDGFK 290 + R H+ AE MR+QA HD LTGL R + A +A ++ + + F+DLD FK Sbjct: 252 ITERKAHE-AE-MRHQATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFK 309 Query: 291 VINDAYGHDAGDQVLVEVARRLQGCLPAGATLARLGGDEFVILYALDAQAE--LPELLAR 348 IND+ GH GD++L VA+RLQ CL +AR GGDEFV++ + E + E++ R Sbjct: 310 FINDSLGHQVGDELLKTVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQR 369 Query: 349 RLIEVVRQPFARLAHRHIGLSLGITL--SDRDDIDEMLVCSDAAMYQAKRRGRNQFCVFN 406 L V R A + S+G++ +D D++ +L +D+AMY+AK +GRN F F Sbjct: 370 ILAAVARPWQAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFA 429 Query: 407 ETLNAHLQMKRDVERDLAGALARRELAVWFQPIVQHDGQTLDGFEALLRWQHGKHGAIAA 466 +++ + + ++ +L AL + E +++QP + L G EAL+RW+ + G + Sbjct: 430 PWMDSQVSNRLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPP 489 Query: 467 PDLIEIAALAGLSEELLRFILEEVAGMIAFLQSSGRSDLRISMNISPREMERLVVDELVV 526 I A AGL + ++L Q +G + +++N+SPR++ + + D V Sbjct: 490 DRFIPFAEEAGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQSLPD-FVA 548 Query: 527 SMLKAHRLPARMLEIEITEETAL-DLQAATQTLVALALQGVRIAIDDFGVGYSSLGFLRQ 585 +L L A LE+EITE + D + + TL AL G++I++DDFG GYSSL +LR+ Sbjct: 549 GVLAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRR 608 Query: 586 MQVSRVKIDRSFVTGLADQTENQALVSAVLQLAGSFGFQVVAEGVESAEDLQTLQAMHCH 645 V +KID+SFV + ++ A+V A++ LA +V+AEGVE E L+ C Sbjct: 609 FPVDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACD 668 Query: 646 AMQGYYFAPPMPAAQARARIEALAQA 671 +QGYYF PM A A + AQA Sbjct: 669 EVQGYYFGKPMEVAVFTAWMSEQAQA 694 >gi|300310170|ref|YP_003774262.1| diguanylate cyclase/phosphodiesterase A protein [Herbaspirillum seropedicae SmR1] Length = 1002 Score = 238 bits (606), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 162/428 (37%), Positives = 235/428 (54%), Gaps = 22/428 (5%) Query: 245 RMRYQAEHDLLTGLLGRAGFMA-AAQAMAA--RREEVLCMMFLDLDGFKVINDAYGHDAG 301 R+ A +D LTGL R+ + A QA+AA R + +MF+DLD FK +ND+ GH AG Sbjct: 553 RIHQLAFYDSLTGLPNRSLLLVQAGQAIAAAERNCSTVAVMFIDLDRFKQVNDSLGHQAG 612 Query: 302 DQVLVEVARRLQGCLPAGATLARLGGDEFVILYALDAQAELPELLARRLIEVVRQPFARL 361 D++L VA RLQ + RL GDEFV++ L + + L EVV++ RL Sbjct: 613 DELLRTVATRLQSQARRSDIVGRLSGDEFVVV--------LTQYDTQHLTEVVKRIQERL 664 Query: 362 AHR-HIG-------LSLGITL--SDRDDIDEMLVCSDAAMYQAKRRGRNQFCVFNETLNA 411 A HI S+G+ + + +DI+ +L +D AMYQAK R +F F+E +N+ Sbjct: 665 AMPCHINGTPLNPSASIGVAIFPDNGNDIETLLHRADIAMYQAKAVNRGRFSFFSEEMNS 724 Query: 412 HLQMKRDVERDLAGALARRELAVWFQPIVQHDGQTLDGFEALLRWQHGKHGAIAAPDLIE 471 Q + +E L AL +L + FQP ++ Q+L G EAL RW H + G I+ I Sbjct: 725 MAQERLALESALRDALRGGDLELNFQPQIKLHDQSLHGVEALARWHHPQLGQISPGRFIP 784 Query: 472 IAALAGLSEELLRFILEEVAGMIAFLQSSGRSDLRISMNISPREMERLVVDELVVSMLKA 531 IA GL EL ++ LEE ++ + G IS+N+S L + + V +L+ Sbjct: 785 IAEECGLIGELGKWALEEACFQLSDWRRRGIQVPSISVNLSATNFHDLDLPQFVTGLLRR 844 Query: 532 HRLPARMLEIEITEETALDLQAAT-QTLVALALQGVRIAIDDFGVGYSSLGFLRQMQVSR 590 + L L +EITE +D +T QT+ A+ G+ +A+DDFG GYSSLG+LR++ VS Sbjct: 845 YALEPSDLTLEITETVLMDTNPSTSQTIAAVHALGIPLAMDDFGTGYSSLGYLRKLPVSE 904 Query: 591 VKIDRSFVTGLADQTENQALVSAVLQLAGSFGFQVVAEGVESAEDLQTLQAMHCHAMQGY 650 +K+D+SFV +A AL +AV+++ S VVAEGVE AE L + C QGY Sbjct: 905 LKLDQSFVRDMAKDPAVSALTNAVIRIGESLKLTVVAEGVEDAEQYALLISQGCDVAQGY 964 Query: 651 YFAPPMPA 658 F+ PM A Sbjct: 965 LFSRPMSA 972 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 231 bits (590), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 143/429 (33%), Positives = 231/429 (53%), Gaps = 7/429 (1%) Query: 239 LHQFAERMRYQAEHDLLTGLLGR---AGFMAAAQAMAARREEVLCMMFLDLDGFKVINDA 295 + ++ + +QA D LTGL+ R + A +A R+++ L ++F+DLD FK +ND Sbjct: 508 MKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDT 567 Query: 296 YGHDAGDQVLVEVARRLQGCLPAGATLARLGGDEFVILYALDAQAELPELLARRLIEVVR 355 GH AGD +L VA RL+ + T+ARLGGDEF+++ + L +R+++ V Sbjct: 568 LGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVA 627 Query: 356 QPFARLAHR-HIGLSLGITLSDRDDID-EMLVC-SDAAMYQAKRRGRNQFCVFNETLNAH 412 P H + S+G+ D D E L+ +D AMY+ K+ GRN F + +N Sbjct: 628 APVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQ 687 Query: 413 LQMKRDVERDLAGALARRELAVWFQPIVQHDGQTLDGFEALLRWQHGKHGAIAAPDLIEI 472 + +E DL AL E + +QP V + G EAL+RWQH + G ++ I + Sbjct: 688 ALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGL 747 Query: 473 AALAGLSEELLRFILEEVAGMIAFLQSSGRSDLRISMNISPREMERLVVDELVVSMLKAH 532 A GL + ++L Q G +D+ +S+N+S + + + + +L+ Sbjct: 748 AEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDT 807 Query: 533 RLPARMLEIEITEETAL-DLQAATQTLVALALQGVRIAIDDFGVGYSSLGFLRQMQVSRV 591 LP LE+E+TE + D++ A L L GVR++IDDFG GYSSL +L++ + + Sbjct: 808 GLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVL 867 Query: 592 KIDRSFVTGLADQTENQALVSAVLQLAGSFGFQVVAEGVESAEDLQTLQAMHCHAMQGYY 651 KIDRSFV + ++ A+ ++++ LA +V+AEGVE+ E L L+ C +QGY Sbjct: 868 KIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYL 927 Query: 652 FAPPMPAAQ 660 F+PP+PA + Sbjct: 928 FSPPVPADK 936 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,847,686 Number of extensions: 73983 Number of successful extensions: 497 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 412 Number of HSP's successfully gapped: 45 Length of query: 671 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 579 Effective length of database: 1,184,250 Effective search space: 685680750 Effective search space used: 685680750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 134 (56.2 bits)