BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0227.p (376 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane ... 725 0.0 gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 89 8e-19 gi|300311362|ref|YP_003775454.1| HlyD family secretion, possible... 70 2e-13 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 69 4e-13 gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbas... 69 7e-13 >gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 376 Score = 725 bits (1871), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/376 (98%), Positives = 372/376 (98%) Query: 1 VKPVSPSVSPGRARRRVYPLLTLVIALAACSKPAPVADAPREVVVLQAQQRGQAGTLHLP 60 +KPVSPSVSPGRARRRVYPLL LVI LAACSKPAPVADAPREVVVLQAQQRGQAGTLHLP Sbjct: 1 MKPVSPSVSPGRARRRVYPLLALVITLAACSKPAPVADAPREVVVLQAQQRGQAGTLHLP 60 Query: 61 AEVQSRYVTSMSFRIAGQLAERRVHLGDVVRKGQVLARLDPADANQNASAAQAELASARQ 120 AEVQSRYVTSMSFRIAGQLAERRVHLGDVVRKGQVLARLDPADANQNASAAQAELASARQ Sbjct: 61 AEVQSRYVTSMSFRIAGQLAERRVHLGDVVRKGQVLARLDPADANQNASAAQAELASARQ 120 Query: 121 HLDAAEKQLQRDVQQAREALISAQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLV 180 HLDAAEKQLQRDVQQAREALIS QQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLV Sbjct: 121 HLDAAEKQLQRDVQQAREALISPQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLV 180 Query: 181 AQHDGVISAEQANTGDVLAAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAAVVTLAAL 240 AQHDGVISAEQANTGDVLAAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAAVVTLAAL Sbjct: 181 AQHDGVISAEQANTGDVLAAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAAVVTLAAL 240 Query: 241 PGKTFQGKVREVSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSAAPVLLLP 300 PGKTF GKVREVSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSAAPVLLLP Sbjct: 241 PGKTFTGKVREVSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSAAPVLLLP 300 Query: 301 ATALFHQGDRPALWVVGEQGKLELRPVTVAAYGERSISVSQGVTASDKVVVQGVHTLTAG 360 ATALFHQGDRPALWVVGEQG+LELRPVTVAAYGERSISVSQGVTASDKVVVQGVHTLTAG Sbjct: 301 ATALFHQGDRPALWVVGEQGQLELRPVTVAAYGERSISVSQGVTASDKVVVQGVHTLTAG 360 Query: 361 EKVKPVAPLHAEDFAL 376 EKVKPVAPLHAEDFAL Sbjct: 361 EKVKPVAPLHAEDFAL 376 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 88.6 bits (218), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 82/312 (26%), Positives = 148/312 (47%), Gaps = 5/312 (1%) Query: 56 TLHLPAEVQSRYVTSMSFRIAGQLAERRVHLGDVVRKGQVLARLDPADANQNASAAQAEL 115 TL LP +Q + +S R G L +G V+KG++LA LD + +Q + A A Sbjct: 83 TLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQALAAR 142 Query: 116 ASARQHLDAAEKQLQRDVQQAREALISAQQLEQSQDAHAAALSQFKAAQARAAVAGNQRD 175 A ++ A + R ++ +S Q+L++ + A A A + AA A Sbjct: 143 EQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLAAADANVGRLRQVEG 202 Query: 176 YTTLVAQHDGVISAEQANTGDVL--AAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAA 233 + +VA G+I+ + GD++ AG+ ++ L+ + + V V ++PG Sbjct: 203 FKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQSYANLVKPGQQV 262 Query: 234 VVTLAALPGKTFQGKVREVSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSA 293 VT + L G+ F+GKV + A DA +R+ +V+VTL D A+ G +VA+ P S Sbjct: 263 TVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQVAL-PIKPSG 321 Query: 294 APVLLLPATALFHQGDRPALWVVGEQGKLELRPVTVAAYGERSISVSQGVTASDKVVVQG 353 A LL+ A L + D + V ++ L+ + + +++ V G+ + +++ Sbjct: 322 A--LLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGLQGDEHLILNP 379 Query: 354 VHTLTAGEKVKP 365 L G++V+P Sbjct: 380 SDALAEGDQVEP 391 >gi|300311362|ref|YP_003775454.1| HlyD family secretion, possible efflux component [Herbaspirillum seropedicae SmR1] Length = 407 Score = 70.1 bits (170), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 86/330 (26%), Positives = 146/330 (44%), Gaps = 60/330 (18%) Query: 70 SMSFRIAGQLAERRVHLGDVVRKGQVLARLDP-ADAN---------QN---------ASA 110 S+ +++GQ+ +V LGD V+KGQ++A +D AN QN AS Sbjct: 67 SVGAQVSGQIKSLKVALGDQVKKGQLVAEIDSLTQANALASAEFSLQNLQAQLRAKEASL 126 Query: 111 AQAELASARQHLDAAEKQLQRDVQQAREALISAQQLEQSQDAHAAAL-SQFKAAQARAAV 169 QA+LA ARQ + A R+ ++ EA ++ Q A AAL +Q K + Sbjct: 127 KQAQLAYARQKMMLAGDASSRENFESAEATLNTTQ------ADIAALQAQIKDGAIKVDT 180 Query: 170 AGNQRDYTTLVAQHDGVISAEQANTGDVLAAGQPVYSLAWSGQVDVVT---EVADRQVAT 226 A YT + + +G + A A G + A Q ++ ++D VT ++++ V Sbjct: 181 ARLNLGYTRISSPIEGQVVAIVAQEGQTVNANQSTPTIIKVARMDTVTIKAQISEADVVR 240 Query: 227 LQPGAAAVVTLAALPGKTFQGKVREVSPAADA---------------------QSRTYRV 265 ++PG T+ P ++ +R + PA D+ + Y Sbjct: 241 VKPGQPVFFTILGDPDHRYRTTLRAIEPAPDSILQDDTSSSTTSITSSSSASSTAIYYNG 300 Query: 266 KVTLEQPDPAVRLGMTGEVAITPAASSAAPVLLLPATALFHQGDRPA-----LWVVGEQG 320 + + PD +R+ MT +V I S A+ L+LP+TAL G + A + V+ +QG Sbjct: 301 LLDVPNPDGKLRISMTTQVNIV--LSEASNALVLPSTAL---GAKAADGSYTVRVLDDQG 355 Query: 321 KLELRPVTVAAYGERSISVSQGVTASDKVV 350 + R V + + + +GV A ++VV Sbjct: 356 QAHERKVRIGINTNALVQIVEGVKAGERVV 385 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 69.3 bits (168), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 95/372 (25%), Positives = 153/372 (41%), Gaps = 26/372 (6%) Query: 18 YPLLTLVIALAA----CS-KPAPVADA--PREVVVLQAQQRGQAGTLHLPAEVQSRYVTS 70 + LT+ +A+AA C KPA P EV V+ + A T LP V++ V Sbjct: 7 FTRLTMTVAIAAALVACGQKPAAQGGPMPPSEVSVVTIAPQRVAITNELPGRVEAFRVAQ 66 Query: 71 MSFRIAGQLAERRVHLGDVVRKGQVLARLDPADANQNASAAQAELASARQHLDAAEKQLQ 130 + R+AG + +R G V++GQVL ++DPA + +AQA LA A+ +L Q Sbjct: 67 VRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQ 126 Query: 131 RDVQQAREALISAQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLVAQHDGVISAE 190 R +S Q+ + + A A + A +A A Y T+ + G I Sbjct: 127 RYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIGRA 186 Query: 191 QANTGDVLAAG-----------QPVYSLAWSGQVDVVT---EVADRQVATLQPGAAAVVT 236 G ++ G PVY D++ +A Q+ ++ A+ VT Sbjct: 187 LVTEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKAS-VT 245 Query: 237 LAALPGKTF--QGKVREVSPAADAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSAA 294 L G+ + GK+ + D + + ++ P + G I A A Sbjct: 246 LELENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQA 305 Query: 295 PVLLLPATALFHQGDRPALWVVGEQGKLELRPVTVAAYGERSISVSQGVTASDKVVVQGV 354 LL+P A+ + VV + K+E RPV + V G+ D+V+V+G+ Sbjct: 306 --LLVPQQAVVRDAGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGL 363 Query: 355 HTLTAGEKVKPV 366 + G VKPV Sbjct: 364 QKVRPGATVKPV 375 >gi|300312743|ref|YP_003776835.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 402 Score = 68.6 bits (166), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 82/301 (27%), Positives = 133/301 (44%), Gaps = 25/301 (8%) Query: 74 RIAGQLAERRVHLGDVVRKGQVLARLDPA-----DANQNASAA--QAELASARQHLDAAE 126 ++AGQ+++ V +G+ V+ G++LA + PA N A+ A +AELA LD A+ Sbjct: 89 KVAGQISDVDVVVGETVKAGKLLATVAPAVQSSRIENNRATLARLKAELAGQNAQLDFAQ 148 Query: 127 KQLQRDVQQAREALISAQQLEQSQDAHAAALSQFKAA-----QARAAVAGNQ--RDYTTL 179 Q QR Q E + E S+ AA ++ A Q AA+ ++ + T + Sbjct: 149 LQFQRQTQLKAENATREESYESSRMNMYAAAARVDATNAQIQQTEAAIREDEAVQKQTRI 208 Query: 180 VAQHDGVISAEQANTGDVLAAGQP-VYSLAWSGQVDVVTEVADRQVATLQPGAAAVVTLA 238 A G I A G ++AA Q + +A ++ V VA+ V LQ G A T Sbjct: 209 EAPVSGTIVTLNARPGQMVAANQEALMRIADLSRMTVQVPVAEEDVTRLQKGMTAYFTTP 268 Query: 239 ALPGKTFQGKVREVS--PAADA----QSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASS 292 PGK + GK+R++ P D+ + Y V + P + GM+ +V A + Sbjct: 269 GYPGKRWSGKLRQIMLLPTDDSGRQGKKAFYTVFFDVANPSRELMSGMSADVYFVLARAE 328 Query: 293 AAPVLLLPATALFHQG--DRPALWVVGEQGKLELRPVTVAAYGERSISVSQGVTASDKVV 350 P +P T + G + VV GKLE R + + V G+ ++V+ Sbjct: 329 HVPT--IPRTLVTKPGMDGTQTVKVVLADGKLETRKIKIGIRDNERAQVLSGLKEGEQVL 386 Query: 351 V 351 + Sbjct: 387 L 387 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,025,237 Number of extensions: 38231 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 266 Number of HSP's successfully gapped: 11 Length of query: 376 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 289 Effective length of database: 1,207,925 Effective search space: 349090325 Effective search space used: 349090325 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)