BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0225.p (489 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprot... 848 0.0 gi|300311703|ref|YP_003775795.1| ABC-type export system outer me... 211 1e-55 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 139 6e-34 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 134 1e-32 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 133 4e-32 >gi|300309611|ref|YP_003773703.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 493 Score = 848 bits (2191), Expect = 0.0, Method: Compositional matrix adjust. Identities = 483/493 (97%), Positives = 487/493 (98%), Gaps = 4/493 (0%) Query: 1 MSQVHPPLPLALLLVLTLSGCAFGPTGEAPAMPQPDHYAAQATPADVGPEGARQRFVLGQ 60 M+QVHPPLPLALLLVLTLSGCAFGPTGEAPAMP+PDHYAAQATPADVGPEGARQRFVLGQ Sbjct: 1 MTQVHPPLPLALLLVLTLSGCAFGPTGEAPAMPRPDHYAAQATPADVGPEGARQRFVLGQ 60 Query: 61 RAVPQWWQAYASPELSAWVEEALRNNHSLAQAERNLASARLQLRAQVDQSLWPSVDAVGQ 120 RAVPQWWQAY SPELSAWVEEALRNNHSLAQAERNLASARLQLRAQ+DQSLWPSVDAVGQ Sbjct: 61 RAVPQWWQAYGSPELSAWVEEALRNNHSLAQAERNLASARLQLRAQIDQSLWPSVDAVGQ 120 Query: 121 AQRQRALGLPNIGPTTNLYNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAAR 180 AQRQRALGLPNIGPTTNLYNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAAR Sbjct: 121 AQRQRALGLPNIGPTTNLYNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAAR 180 Query: 181 RALVANVVISAINAAALRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARTNAQ 240 RALVANVVISAINAAALRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAAR NAQ Sbjct: 181 RALVANVVISAINAAALRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARANAQ 240 Query: 241 SLRASLPGLQAQWLAARHALAVLMGRTPDQAPADLDLASLRLPAEVPLAVPSELLRQRPD 300 SLRASLPGLQAQWLAARHALAVLMGRTPDQAPADLDLASLRLPAEVPLAVPSELLRQRPD Sbjct: 241 SLRASLPGLQAQWLAARHALAVLMGRTPDQAPADLDLASLRLPAEVPLAVPSELLRQRPD 300 Query: 301 ILAADAALKVAAAQQAQATAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPL 360 ILAADAALKVAAAQQAQATAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPL Sbjct: 301 ILAADAALKVAAAQQAQATAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPL 360 Query: 361 FHGGALRSMSEAAEQAYLAAEANYRQTVLNAFQNVADSLVALSHDADAQTAAAAARQASE 420 FHGGALRSMSEAAEQAYLAAEANYRQTVLNAFQNVADSLVALSHDADAQTAAAAARQASE Sbjct: 361 FHGGALRSMSEAAEQAYLAAEANYRQTVLNAFQNVADSLVALSHDADAQTAAAAARQASE 420 Query: 421 QGWRNAQRRVALGAVPVSAARSGERQYLNAHLSEARATASRLNATATLFQAMGVSLEAAP 480 QGWRNAQRRVALGAVP+SAARSGERQYLNAHLSEARATASRLNATATLFQAMGVSLEAAP Sbjct: 421 QGWRNAQRRVALGAVPISAARSGERQYLNAHLSEARATASRLNATATLFQAMGVSLEAAP 480 Query: 481 A----TAPQAAQP 489 A TAPQAAQP Sbjct: 481 ATSPTTAPQAAQP 493 >gi|300311703|ref|YP_003775795.1| ABC-type export system outer membrane channel protein [Herbaspirillum seropedicae SmR1] Length = 489 Score = 211 bits (537), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 168/477 (35%), Positives = 240/477 (50%), Gaps = 24/477 (5%) Query: 10 LALLLVLTLSGCAFGPTGEAPAMPQPDHYAAQATPADVGPE-GARQRFVLGQRAVPQWWQ 68 LA +L+ T +GC GP AP P+ Y D E Q+ ++G+ +WWQ Sbjct: 13 LAWMLLATQAGCLVGPDFVAPTPPRVSQYTRDPVLLDRMSEPDMSQQLLMGENPPARWWQ 72 Query: 69 AYASPELSAWVEEALRNNHSLAQAERNLASARLQLRAQVDQSLWPSVDAVGQAQRQRALG 128 + SPEL A V +AL N +L A NL A LRA +P +DA A+R R+ Sbjct: 73 LFGSPELDAAVTQALTRNPTLQAAVSNLLLAEDNLRAG-GAVFYPRLDANMMAERTRSAS 131 Query: 129 LPNIGPTTN-LYNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAARRALVANV 187 L + +++V SY DLFG +R A VD Q AA L ANV Sbjct: 132 LVQGSSAPDKVFSVATLSGAVSYAIDLFGEKRRTLEQLEAMVDVQQQLHHAAYLTLTANV 191 Query: 188 VISAINAAALRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARTNAQSLRASLP 247 V + I A Q+S +++AL E +Q R + G A DLL+ + +A L Sbjct: 192 VNAYIARAGYLSQISLVRQIIALEELQLEAIQARVDTGVAPLADLLSQASLLAGNQALLA 251 Query: 248 GLQAQWLAARHALAVLMGRTPDQAPA-DLDLASLRLPAEVPLAVPSELLRQRPDILAADA 306 L + H LA L G P QA +L LA L LP+++P+++PS L+R+RPDILAA+A Sbjct: 252 QLTQRLYQIEHLLAQLQGEFPAQASLPELVLADLHLPSDIPVSLPSALVRRRPDILAAEA 311 Query: 307 ALKVAAAQQAQATAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPLFHGGAL 366 + A+A ++A +PSL ++A++G + + TG A ++W G SL+ PLF+GG L Sbjct: 312 RMHAASAAIGMSSAAQYPSLELTATYGVAARNLSQLTGPATSLWSFGPSLSTPLFNGGRL 371 Query: 367 RSMSEAAEQAYLAAEANYRQTVLNAFQNVADSLVAL---SHDADAQTAAAAARQASEQGW 423 R+ +AA A+ A+++ Y+QTVL AF+ VAD+L AL H AQ+AAA Sbjct: 372 RAQRDAAVNAFEASQSEYQQTVLTAFEQVADALRALQADGHIVSAQSAAA---------- 421 Query: 424 RNAQRRVALGAVPVSAARSG-------ERQYLNAHLSEARATASRLNATATLFQAMG 473 R A + L SA S E QY +A ++ +AT R T L A G Sbjct: 422 RYAYDTLLLQRASYSAGLSSYADMLLYEVQYRSAAIASLQATVQRYQDTVALLVASG 478 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 139 bits (349), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 144/473 (30%), Positives = 207/473 (43%), Gaps = 43/473 (9%) Query: 20 GCAFGPTGEAPAMPQPDHYAAQA-----TPADVGPEGARQRFVLGQRAVPQWWQAYASPE 74 GCA GP P + P + ++A PAD P G WW+ + + Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGP-------------WWERFGDAQ 64 Query: 75 LSAWVEEALRNNHSLAQAERNLASARLQLRAQVDQSLWPSVDAVGQAQRQRA-------- 126 L+A ++AL N +LA A LA AR QL + P V+ +A R R Sbjct: 65 LNALQQQALAGNQTLAIATARLAQARAQLNV-TGAAQTPQVNLNARAARSRISANRPLTN 123 Query: 127 LGLPNIGPTTNLYNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQFDAARRALVAN 186 PN + + N FA SY D FG + + A A + + + R L A Sbjct: 124 YNSPNF---STVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAE 180 Query: 187 VVISAINAAALRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARTNAQSLRASL 246 + + N L V++ R +AL R +LGA S D+ + S + Sbjct: 181 LATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQI 240 Query: 247 PGLQAQWLAARHALAVLMGRTPDQAPADLDLASLR--LPAEVPLAVPSELLRQRPDILAA 304 L Q HA+A L G TP APA SL P +P+ VPS++L +RPDI AA Sbjct: 241 DLLGRQRAQYEHAIATLTG-TP--APAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAA 297 Query: 305 DAALKVAAAQQAQATAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPLFHGG 364 + A+ A AQ A+A +PS + S+G W A +W LG S +Q LF GG Sbjct: 298 ERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGG 357 Query: 365 ALRSMSEAAEQAYLAAEANYRQTVLNAFQNVADSLVALS----HDADAQTAAAAARQASE 420 LR+ + ++ Y A A+YR+TVL A Q V D + L+ A +Q A A+AR+ E Sbjct: 358 RLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLE 417 Query: 421 QGWRNAQRRVALGAVPVSAARSGERQYLNAHLSEARATASRLNATATLFQAMG 473 A R GA P + ++ LN+ A+ RL + L +A+G Sbjct: 418 ----IASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALG 466 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 134 bits (338), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 146/479 (30%), Positives = 217/479 (45%), Gaps = 43/479 (8%) Query: 20 GCAFGPTGEAPAMPQPDHYAAQATPADVGPEGARQRFVLGQRAVP--QWWQAYASPELSA 77 GC+ PT E P++ P + A G G++ + + +WW+ + +L+A Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 78 WVEEALRNNHSLAQAERNLASARLQLRAQVDQSLWPSVDAVGQAQRQR----ALGLP--- 130 + A + N L A L AR R +P VDA A RQR + GLP Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTR-DARAGYFPQVDAGVGATRQRPSPASQGLPADA 148 Query: 131 NIGPTTNLYNVFAGQIRASYTFDLFGTERYANAATAAQVDAQSYQ--FDAARRALVANV- 187 + P T ++ Q+ SY DLFG R A+ AA DAQ + F + AL A+V Sbjct: 149 DTRPNT----LYRAQVNVSYEVDLFG--RVASTVDAATADAQRSEALFHSVLLALQADVA 202 Query: 188 ----VISAINAAALRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARTNAQSLR 243 ++ ++AAA + + V L + VQRR + G S+ D+ AR S + Sbjct: 203 QQYFLVRELDAAAALYRGT-----VDLRQESLRLVQRRFDEGDISEVDVARARAELASAQ 257 Query: 244 ASLPGLQAQWLAARHALAVLMGRTPDQAPADLDLAS---LRLPAEVPLAVPSELLRQRPD 300 + G+ Q A HALA+L+GRTP A+ L RL VP +PS LL +RPD Sbjct: 258 SEALGIARQRATAEHALAILLGRTP----AEFSLPPQPLTRLAVTVPPGLPSALLERRPD 313 Query: 301 ILAADAALKVAAAQQAQATAEMFPSLSISASFGQSG------FTWPKATGAAGAVWGLGA 354 I AA+ A+ A A+ A + FPSL+++ G F W T G + +G Sbjct: 314 IAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPL--VGT 371 Query: 355 SLTQPLFHGGALRSMSEAAEQAYLAAEANYRQTVLNAFQNVADSLVALSHDADAQTAAAA 414 +L+ PLF GG ++ + A +Y A+YR TVLNAF+ V D+L L A Sbjct: 372 ALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDE 431 Query: 415 ARQASEQGWRNAQRRVALGAVPVSAARSGERQYLNAHLSEARATASRLNATATLFQAMG 473 A AS + + + + GAV + +R L + R +T L +A+G Sbjct: 432 AVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALG 490 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 133 bits (334), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 136/477 (28%), Positives = 213/477 (44%), Gaps = 33/477 (6%) Query: 11 ALLLVLTLSGCAFGPTGEAPAMPQPDHYAAQATPADVGPEGARQRFVLGQRAVPQWWQAY 70 AL++ L GC+ PT E P P Y PAD Q LG W Q + Sbjct: 18 ALVMAGVLGGCSLAPTYERPQAPVESSY-----PADAAGRAELQAVNLG------WRQFF 66 Query: 71 ASPELSAWVEEALRNNHSLAQAERNLASARLQLR-AQVDQSLWPSVDAVGQAQRQRA--- 126 L + AL NN L A N+ AR Q + A+ D L P+++ G A R R Sbjct: 67 PDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARAD--LLPNLNLSGNAARTRTAAS 124 Query: 127 ---LGLPNIGPTTNLYNVFAGQIRASYTFDLFGTER-YANAATAAQVDAQSYQFDAARRA 182 G P I +N Y V G +Y D FG R +AA A+ + + Q +A+ + Sbjct: 125 QSLTGQPTI---SNSYQV--GLAVPAYELDFFGRVRNLKDAALASYLSTEEAQ-KSAQIS 178 Query: 183 LVANVVISAINAAALRVQVSDTERLVALAEADASEVQRRAELGAASQDDLLAARTNAQSL 242 LVA V + + + Q+ + + +++ Q+R ++GA++ D +T +S Sbjct: 179 LVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESA 238 Query: 243 RASLPGLQAQWLAARHALAVLMGRTPDQAPADLDLASLRLPAEVPLAVPSELLRQRPDIL 302 R + L+ Q A +AL +L+G+ + P L+ ++ ++P +PS+LL RPDI Sbjct: 239 RVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIR 298 Query: 303 AADAALKVAAAQQAQATAEMFPSLSISASFGQSGFTWPKATGAAGAVWGLGASLTQPLFH 362 +A+ +L A A A A FP +S++ +FG + T + W G SL+ P+F Sbjct: 299 SAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFD 358 Query: 363 GGALRSMSEAAEQAYLAAEANYRQTVLNAFQNVADSLVA---LSHDADAQTAAAAARQAS 419 G + + AE A Y + + AF+ VAD+LVA L AQ A AA+Q Sbjct: 359 FGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQ-- 416 Query: 420 EQGWRNAQRRVALGAVPVSAARSGERQYLNAHLSEARATASRLNATATLFQAMGVSL 476 + + R G +R +A + +A RLN L++A+G L Sbjct: 417 -ERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGL 472 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.125 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,238,251 Number of extensions: 46516 Number of successful extensions: 341 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 18 Length of query: 489 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 400 Effective length of database: 1,198,455 Effective search space: 479382000 Effective search space used: 479382000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)