BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0223.p (293 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease ... 581 e-167 gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease ... 116 3e-27 gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC tra... 108 8e-25 gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease ... 102 4e-23 gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease ... 93 3e-20 >gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 581 bits (1498), Expect = e-167, Method: Compositional matrix adjust. Identities = 289/293 (98%), Positives = 290/293 (98%) Query: 1 VATTAASGPQRSAARRNRAAFWFLAPACVMTLVYVLYPILYTIYLSFFSWDGMTAPKFVG 60 VATTAASGPQRSAARRNRAAFWFLAPACVMTLVYVLYPILYTIYLSFFSWDGMT P+FVG Sbjct: 19 VATTAASGPQRSAARRNRAAFWFLAPACVMTLVYVLYPILYTIYLSFFSWDGMTEPRFVG 78 Query: 61 LANYVELFHAPTFYTALKNNVLWLLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFV 120 LANYVELFHAPTFYTALKNNVLWLLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFV Sbjct: 79 LANYVELFHAPTFYTALKNNVLWLLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFV 138 Query: 121 LSGVVVGLMYSWFYDPSFGLLSLLFGQGVPVLGDARYATFGIIFAALWPQTAYCMILFLT 180 LSGVVVGLMYSWFYDPSFGLLSLLFGQGVPVLGDARYATFGIIFAALWPQTAYCMILFLT Sbjct: 139 LSGVVVGLMYSWFYDPSFGLLSLLFGQGVPVLGDARYATFGIIFAALWPQTAYCMILFLT 198 Query: 181 GLTALNHDQVEAARMEGAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSG 240 GLTALNHDQVEAARMEGAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSG Sbjct: 199 GLTALNHDQVEAARMEGAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSG 258 Query: 241 GPYESSTVLAYYAYDQAIKYYRQGYSAAVAVTLFAIMLVYIVYQLRRALNAER 293 GPYESSTVLAYYAYDQAIKYYRQGYSAAVAVTLFAIMLVYIVYQLRR L AER Sbjct: 259 GPYESSTVLAYYAYDQAIKYYRQGYSAAVAVTLFAIMLVYIVYQLRRMLRAER 311 >gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 291 Score = 116 bits (290), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 87/249 (34%), Positives = 133/249 (53%), Gaps = 8/249 (3%) Query: 24 LAPACVMTLVYVLYPILYTIYLSFFSW-DGMTAPKFVGLANYVELFHAPTFYTALKNNVL 82 L+PA V+ + + YP L T++ SFFS G+ FVGL NY + P F+ AL NN L Sbjct: 14 LSPAMVLLIAFTHYPALATLWHSFFSTPKGVRPSVFVGLENYRLMADDPVFWQALCNN-L 72 Query: 83 WLLMFLLAPPMGLAI--ALYLNQKVIGMRVVKSLFFAPFVLSGVVVGLMYSWFYDPSFGL 140 W + + + LAI AL++N+K+ G V++ +F P VL + V ++ +FY P +GL Sbjct: 73 WFALGTIPLAIALAILMALWVNEKLAGRGVLRLAYFTPTVLPMIAVANIWLFFYTPQYGL 132 Query: 141 LSLLFGQ-GVPV---LGDARYATFGIIFAALWPQTAYCMILFLTGLTALNHDQVEAARME 196 L + G G+P LG+ + ++ A+W + + MI +L L ++ EAA +E Sbjct: 133 LEQITGALGLPSHNWLGNPQTVLGALMVVAVWKEAGFFMIFYLAALQQISPVLAEAAALE 192 Query: 197 GAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTSGGPYESSTVLAYYAYDQ 256 GA W V P L TT + ++I A R D I VMT GGP +S++L YY Y+ Sbjct: 193 GASRWQYFWRVQFPLLMPTTLFVLINALINAFRLVDHIVVMTKGGPDNASSLLLYYIYEV 252 Query: 257 AIKYYRQGY 265 ++ Y Sbjct: 253 GFAFWDSSY 261 >gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 108 bits (269), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 75/269 (27%), Positives = 139/269 (51%), Gaps = 6/269 (2%) Query: 24 LAPACVMTLVYVLYPILYTIYLSFFSWDGMTA-PKFVGLANYVELFHAPTFYTALKNNVL 82 +AP ++TL++ +P + +Y S D +FV N+ LF PT+ + + + Sbjct: 17 VAPQIIITLLFFFWPAVQALYQSMLLQDAFGGYSEFVWFDNFKTLFGDPTYLESFRTTAI 76 Query: 83 W-LLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFVLSGVVVGLMYSWFYDPSFGLL 141 + L+ + L +A++ ++ G + K+ P+ +S VVVG+++ + PS G+L Sbjct: 77 FSALVAFFGLALSLLLAVFADRVRRGASIYKTFLIWPYAVSPVVVGVLWMFLLSPSLGIL 136 Query: 142 S-LLFGQGVP--VLGDARYATFGIIFAALWPQTAYCMILFLTGLTALNHDQVEAARMEGA 198 S +L G+P + + +A I+ AA+W Q +Y + FL GL ++ +EAA ++GA Sbjct: 137 SHVLSWLGLPWDYMINGTHAMILIVIAAIWKQISYNFLFFLAGLQSIPKSLIEAAAIDGA 196 Query: 199 KGWTMLRYVILPQLRSTTFMAFVVSIIGA-LRSFDLISVMTSGGPYESSTVLAYYAYDQA 257 ++ P + TTF FVV+++ A +F ++ T GGP + + +L Y + Sbjct: 197 GPVKRFFTIVFPLISPTTFFLFVVNVVYAFFDTFVIVDATTHGGPGKDTEILVYKVFSDG 256 Query: 258 IKYYRQGYSAAVAVTLFAIMLVYIVYQLR 286 K G SAA +V L A++++ V Q + Sbjct: 257 FKGGDLGGSAAQSVVLMALVILLTVVQFK 285 >gi|300312636|ref|YP_003776728.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 299 Score = 102 bits (254), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 78/286 (27%), Positives = 132/286 (46%), Gaps = 15/286 (5%) Query: 10 QRSAARRNRAAFWFLAPACVMTLVYVLYPILYTIYLSFFSWDGMTAPKFVGLANYVELFH 69 R RN F+APA ++ +V++ YP+ I+L F F+GL N+ L Sbjct: 3 SRFLNNRNVLGMLFMAPAVILLVVFLTYPLGLGIWLGFTDTKIGGEGSFIGLDNFTYLAG 62 Query: 70 APTFYTALKNNVLW-----LLMFLLAPPMGLAIALYLNQKVIGMRVVKSLFFAPFVLSGV 124 +L N V + +L F+L GL +A+ LN+ V +++ P+++ Sbjct: 63 DSLAQLSLFNTVFYTVSASILKFML----GLWLAILLNKNVPLKTFFRAIVLLPWIVPTA 118 Query: 125 VVGLMYSWFYDPSFGLLSL------LFGQGVPVLGDARYATFGIIFAALWPQTAYCMILF 178 + L + W YD F ++S L + + LGD A + +FA +W + I Sbjct: 119 LSALAFWWLYDAQFSVISWALHKMGLIDRYIDFLGDPWNARWSTVFANVWRGIPFVAISL 178 Query: 179 LTGLTALNHDQVEAARMEGAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMT 238 L GL ++ EAA ++GA W R+V LP L + S++ F LI V+T Sbjct: 179 LAGLQTISPSLYEAAAIDGATPWQQFRHVTLPLLTPIIAVVMTFSVLFTFTDFQLIYVLT 238 Query: 239 SGGPYESSTVLAYYAYDQAIKYYRQGYSAAVAVTLFAIMLVYIVYQ 284 GGP ++ ++A ++ +AI G AA+A + +L I++ Sbjct: 239 RGGPLNATHLMATLSFQRAIPGGALGEGAALATYMIPFLLAAIMFS 284 >gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 324 Score = 92.8 bits (229), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 78/287 (27%), Positives = 139/287 (48%), Gaps = 12/287 (4%) Query: 5 AASGPQRSAARRNR--AAFWFLAPACVMTLVYVLYPILYTIYLSFFSWDGMTAPK--FVG 60 A+ Q S R+ R P+ ++ L +++ P+ TIY S ++ M + FVG Sbjct: 23 ASRRQQTSPPRKERFNPIRLMQTPSVLILLAWMIVPLAMTIYFSVNRYNLMAPDQTGFVG 82 Query: 61 LANYVELFHAPTFYTALKNNVLWLLMFLLAPPM--GLAIALYLNQKVIGMRVVKSLFFAP 118 L NY L P F+ ++ N L L+ +LA + G +A+ +Q G + + L P Sbjct: 83 LENYAFLLQDPAFWPSIGNTAL-LIGSVLAISVIGGTLLAVLFDQPFRGRGIARLLVIGP 141 Query: 119 FVLSGVVVGLMY-SWFYDPSFGLLSLLFGQ-GV-PVLGDARYATFGIIFAALWPQTAYCM 175 F + V L++ + P +G+L+ + G+ P+ A Y +I W + Sbjct: 142 FFVMPTVAALIWKNMIMHPVYGILAYVMRHLGLEPIDWLAEYPMLSVIIIVAWQWIPFAF 201 Query: 176 ILFLTGLTALNHDQVEAARMEGAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLIS 235 ++ LT L +L+ DQ EAA+++GA + Y++LP L+ + ++ I L F I Sbjct: 202 LILLTALQSLDTDQKEAAQLDGAGPVRIFFYIVLPHLKRAITVVIMIETIFLLSVFAEIF 261 Query: 236 VMTSGGPYESSTVLAYYAYDQAIKYYRQGYSAAVAVTLFAIMLVYIV 282 T+GGP ++T L + Y ++ + G ++A + FA++L IV Sbjct: 262 TTTAGGPGTATTNLTFLVYSIGLQQFDIGIASAGGI--FAVVLANIV 306 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.141 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 839,885 Number of extensions: 33331 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 10 Length of query: 293 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 209 Effective length of database: 1,222,130 Effective search space: 255425170 Effective search space used: 255425170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)