BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0211.p (326 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 665 0.0 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 168 6e-43 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 167 9e-43 gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 120 2e-28 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 665 bits (1715), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/326 (100%), Positives = 326/326 (100%) Query: 1 MIPVSSATELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFK 60 MIPVSSATELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFK Sbjct: 1 MIPVSSATELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFK 60 Query: 61 FRGAMNALTQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATR 120 FRGAMNALTQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATR Sbjct: 61 FRGAMNALTQFDATQRKHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATR 120 Query: 121 GYGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA 180 GYGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA Sbjct: 121 GYGAEVVTYDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDA 180 Query: 181 LFVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGA 240 LFVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGA Sbjct: 181 LFVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGA 240 Query: 241 QTQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQ 300 QTQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQ Sbjct: 241 QTQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQ 300 Query: 301 LQGKRVGVIISGGNVDLGKLATFLAD 326 LQGKRVGVIISGGNVDLGKLATFLAD Sbjct: 301 LQGKRVGVIISGGNVDLGKLATFLAD 326 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 168 bits (425), Expect = 6e-43, Method: Compositional matrix adjust. Identities = 117/308 (37%), Positives = 169/308 (54%), Gaps = 3/308 (0%) Query: 10 LPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALT 69 + + D+ AA R++ TP + SRT +GAQ+F K EN Q +FK RGA N L Sbjct: 1 MTTLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLV 60 Query: 70 QFDATQR-KHGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVT 128 A+ GV+ S+GNHAQG A AQ LG+PA IVMP+ P K+ TRG+GA VV Sbjct: 61 DVAASGGGARGVVAMSAGNHAQGVAYHAQRLGLPATIVMPRFTPGVKIERTRGFGANVVL 120 Query: 129 YDRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCLGGG 188 + E LA + +T + PYD ++AGQGT LE+++ LD L V +GGG Sbjct: 121 HGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVSIGGG 180 Query: 189 GLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKY 248 GLL+G A+AARA+ P +I GV+ + +++ + H TIA+G G Sbjct: 181 GLLAGMAVAARAIKPEIEIVGVQTRRFPTMVNAVKGTE--HPVGASTIAEGIAVGTPGTL 238 Query: 249 TFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKRVGV 308 T +I D++ V + ++ + E K +VE G G AA + D+ +GKRVG+ Sbjct: 239 TRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRGKRVGL 298 Query: 309 IISGGNVD 316 ++SGGN+D Sbjct: 299 VLSGGNID 306 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 167 bits (424), Expect = 9e-43, Method: Compositional matrix adjust. Identities = 108/308 (35%), Positives = 171/308 (55%), Gaps = 10/308 (3%) Query: 22 RIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNALTQFDATQRKHGVI 81 R+ A TP+ + T + +++FK E+ Q + +FK RGA N + Q K GVI Sbjct: 13 RVYDVAQETPLELANTLSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLKRGVI 72 Query: 82 TFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEVVTY-DRYTEDREAIG 140 S+GNHAQG AL+A LG AVIVMP P K+ A + G EVV + D +T+ E Sbjct: 73 CASAGNHAQGVALSAARLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEH-A 131 Query: 141 ARLAAERKMTLIPPYDHPHVMAGQGTATLELL-QETGPLDALFVCLGGGGLLSGAALAAR 199 L ++K+T + P+D P+V+AGQGT +E+L Q P+ A+FV +GGGGL++G A + Sbjct: 132 LTLEKKQKLTFVHPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVK 191 Query: 200 ALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHLGKYTFGVIRELVDD 259 A+ P KI GV+ + +SL+AG+ V + +DG + +G+ TF + +ELVD+ Sbjct: 192 AVRPDIKIIGVQTTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDE 251 Query: 260 IHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKL-------KDQLQGKRVGVIISG 312 + V + + ++ + + +VEP G L A A+ K ++G+ + I G Sbjct: 252 VIIVDTDAVCTAIKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACG 311 Query: 313 GNVDLGKL 320 N++ +L Sbjct: 312 ANMNFDRL 319 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 120 bits (301), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 102/311 (32%), Positives = 150/311 (48%), Gaps = 4/311 (1%) Query: 8 TELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNA 67 T LPS D+ AA + T L + LGA+++ K EN GAFK RG M Sbjct: 7 TLLPSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVY 66 Query: 68 LTQFDATQRK-HGVITFSSGNHAQGTALAAQLLGIPAVIVMPQDAPAAKVAATRGYGAEV 126 L Q + GV++ + GNH Q LAA+ LGI A IV+PQ K AA R G E+ Sbjct: 67 LHHLARQQPQLSGVVSATRGNHGQSVGLAARRLGISATIVVPQGNSREKNAAMRALGVEL 126 Query: 127 VTY-DRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDALFVCL 185 + + + E RE A ++ + P H ++AG + +EL LD + V + Sbjct: 127 IEHGSEFQESREYAQQLAAQQQLHMI--PSLHRDLVAGVASYWMELFAAQPGLDVVLVPI 184 Query: 186 GGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQTQHL 245 G G + GA A AL + +I GV + S AG+ + IADG + Sbjct: 185 GQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADGVACRVP 244 Query: 246 GKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQLQGKR 305 + + V+ VD++ V+D+E++ M+ V E G AAA +L+ +L+G + Sbjct: 245 DQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRARLRGLK 304 Query: 306 VGVIISGGNVD 316 VGV +SGGNVD Sbjct: 305 VGVTLSGGNVD 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 930,945 Number of extensions: 35729 Number of successful extensions: 128 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 122 Number of HSP's successfully gapped: 4 Length of query: 326 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 241 Effective length of database: 1,217,395 Effective search space: 293392195 Effective search space used: 293392195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)