BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0201.p (296 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309639|ref|YP_003773731.1| LysR family transcription regul... 593 e-171 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 140 2e-34 gi|300311868|ref|YP_003775960.1| LysR family transcription regul... 130 1e-31 gi|300312334|ref|YP_003776426.1| transcription regulator protein... 127 1e-30 gi|300311929|ref|YP_003776021.1| LysR family transcription regul... 125 5e-30 >gi|300309639|ref|YP_003773731.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 296 Score = 593 bits (1530), Expect = e-171, Method: Compositional matrix adjust. Identities = 295/296 (99%), Positives = 296/296 (100%) Query: 1 MLNRLEMLRIFCAAADTGSFKDAAIRLGISPQAVTRAIQELERLQGEMLFHRNTRQVRIT 60 MLNRLEMLRIFCAAADTGSFKDAAIRLGISPQAVTRAIQELERLQGEMLFHRNTRQVRIT Sbjct: 1 MLNRLEMLRIFCAAADTGSFKDAAIRLGISPQAVTRAIQELERLQGEMLFHRNTRQVRIT 60 Query: 61 SFGEALAERARASLRDLDGLFETDTASPADEMSGLVRLAAPSSLGRSRLLPIVERLAQAH 120 SFGEALAERARASLRDLDGLFETDTASPADEMSGLVRLAAPSSLGRSRLLPIVERLAQAH Sbjct: 61 SFGEALAERARASLRDLDGLFETDTASPADEMSGLVRLAAPSSLGRSRLLPIVERLAQAH 120 Query: 121 PGLRFDLRLTDQRADVVGEKIDIGIRVGTVPDNRFVVRRLGKVRFHVVATPRFLRRHGTP 180 PGLRFDLRLTDQRADVVGEKIDIGIRVGTVPDNRFVVRRLGKVRF+VVATPRFLRRHGTP Sbjct: 121 PGLRFDLRLTDQRADVVGEKIDIGIRVGTVPDNRFVVRRLGKVRFYVVATPRFLRRHGTP 180 Query: 181 EDTEALRALPCTAMLDRGTGRPWPWLFAQENQWMADHARFLCDDSEGEYLLVRSGVAIGQ 240 EDTEALRALPCTAMLDRGTGRPWPWLFAQENQWMADHARFLCDDSEGEYLLVRSGVAIGQ Sbjct: 181 EDTEALRALPCTAMLDRGTGRPWPWLFAQENQWMADHARFLCDDSEGEYLLVRSGVAIGQ 240 Query: 241 IAGFLCEDDIAAGRLVPVMTDHEPDPWDMYLYRPQRGPVPPRLRLVFDTLVREFAE 296 IAGFLCEDDIAAGRLVPVMTDHEPDPWDMYLYRPQRGPVPPRLRLVFDTLVREFAE Sbjct: 241 IAGFLCEDDIAAGRLVPVMTDHEPDPWDMYLYRPQRGPVPPRLRLVFDTLVREFAE 296 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 140 bits (352), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 86/298 (28%), Positives = 143/298 (47%), Gaps = 7/298 (2%) Query: 2 LNRLEMLRIFCAAADTGSFKDAAIRLGISPQAVTRAIQELERLQGEMLFHRNTRQVRITS 61 +N+L + F A AD GSF AA LGIS AV+R + ELE G L R TR++ +T Sbjct: 1 MNKLREIECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTE 60 Query: 62 FGEALAERAR---ASLRDLDGLFETDTASPADEMSGLVRLAAPSSLGRSRLLPIVERLAQ 118 G ER + A+L + D L + +A P +GL+R+ AP + G L P+ + Sbjct: 61 AGALYLERCKQIVAALEEADDLLSSGSAKP----TGLLRINAPLTFGVLHLAPLWPMFLE 116 Query: 119 AHPGLRFDLRLTDQRADVVGEKIDIGIRVGTVPDNRFVVRRLGKVRFHVVATPRFLRRHG 178 HP + D+ L+D+ D++ E D+ IR+ + D+ V R+L HV A+P +L HG Sbjct: 117 RHPEISLDITLSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHG 176 Query: 179 TPEDTEALRALPCTAMLDRGTGRPWPWLFAQENQWMADHARFLCDDSEGEYLLVRSGVAI 238 TP L + + W + + + +R ++ + G+ I Sbjct: 177 TPAHPHELAQHQTISYSYNQSKDEWQFNGPEGPVSVRVSSRLHVNNGDSCVQAALGGIGI 236 Query: 239 GQIAGFLCEDDIAAGRLVPVMTDHEPDPWDMYLYRPQRGPVPPRLRLVFDTLVREFAE 296 + F+ + + +G+LV ++ D+ +Y P R +P ++R + D L FA+ Sbjct: 237 TRQPTFMIDQYLRSGQLVSILGDYGAPELGIYAVYPSRAHLPAKMRAMLDFLTEAFAQ 294 >gi|300311868|ref|YP_003775960.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 307 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 91/296 (30%), Positives = 145/296 (48%), Gaps = 11/296 (3%) Query: 2 LNRLEMLRIFCAAADTGSFKDAAIRLGISPQAVTRAIQELERLQGEMLFHRNTRQVRITS 61 +++L+ LRIF A+ SF AA +LG+ +V+ AIQ+LE++ G L R TR+V++T+ Sbjct: 1 MHQLDTLRIFQRVAELASFSGAARQLGLPNASVSLAIQQLEQMLGTRLLQRTTRRVQLTA 60 Query: 62 FGEALAERARASLRDLD---GLFETDTASPADEMSGLVRLAAPSSLGRSRLLPIVERLAQ 118 GEA R+R L + + G+F A +SG +R+ S++ R +LP + Sbjct: 61 DGEAFYRRSRELLDEFESLRGMFHDG----AQALSGRLRVDMSSAMAREVVLPALPSFLD 116 Query: 119 AHPGLRFDLRLTDQRADVVGEKIDIGIRVGTVPDNRFVVRRLGKVRFHVVATPRFLRRHG 178 HP L +L +D+R D+V E D +R GT+ D+ V R +G + A+P +LRR+G Sbjct: 117 KHPQLEIELSASDRRVDLVAEGFDCVLRTGTLDDSSLVARPIGHLVQINCASPEYLRRYG 176 Query: 179 TPEDTEALRALPCTAMLDRGTGRP--WPWLFAQENQWMADHARFLCDDSEGEYLLVRSGV 236 TP+ E L GR W W Q+ + + + +E Y +G+ Sbjct: 177 TPQTLEDLAQHRLVHYRQVLGGRSPGWEWFDGQQTRSLPMAGSITVNSTETYYAACLAGL 236 Query: 237 AIGQ--IAGFLCEDDIAAGRLVPVMTDHEPDPWDMYLYRPQRGPVPPRLRLVFDTL 290 + Q + G + G +V V+ + P P + L R VP RL D L Sbjct: 237 GLIQLPLVGAQKRQMLDGGHVVEVLPQYRPAPMPVSLLYASRRHVPARLAGFMDWL 292 >gi|300312334|ref|YP_003776426.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 127 bits (318), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 85/295 (28%), Positives = 138/295 (46%), Gaps = 1/295 (0%) Query: 2 LNRLEMLRIFCAAADTGSFKDAAIRLGISPQAVTRAIQELERLQGEMLFHRNTRQVRITS 61 +++ + + F A GS AA GI+P + R + LE G L R TR++ +T+ Sbjct: 1 MDQFKQISTFAEVAARGSLSAAARAEGIAPAMIGRRLDALEERLGVKLLQRTTRKIVLTN 60 Query: 62 FGEALAERARASLRDLDGLFETDTASPADEMSGLVRLAAPSSLGRSRLLPIVERLAQAHP 121 G A E + L DL+ E+ + + SG + ++AP+ GR + P++ H Sbjct: 61 EGAAFLEDCQRILADLEDA-ESAVSERSARASGQLLISAPAGFGRQHVAPLLPSFLAEHR 119 Query: 122 GLRFDLRLTDQRADVVGEKIDIGIRVGTVPDNRFVVRRLGKVRFHVVATPRFLRRHGTPE 181 + L L D+ ADV+GE ID+ IR+ T+ D+ V +L + +VA P +LRRHGTP+ Sbjct: 120 DVTLTLNLNDRIADVIGEGIDVAIRIATLTDSNLVSVKLAENHRVIVAAPAYLRRHGTPQ 179 Query: 182 DTEALRALPCTAMLDRGTGRPWPWLFAQENQWMADHARFLCDDSEGEYLLVRSGVAIGQI 241 + L C AM G+ R W + + +C+D E + +G + Sbjct: 180 SPDELMRHNCLAMSSEGSQRGWTLRQSGKVSVYKVGGNMVCNDGEVLHNWALAGRGLAWR 239 Query: 242 AGFLCEDDIAAGRLVPVMTDHEPDPWDMYLYRPQRGPVPPRLRLVFDTLVREFAE 296 + + I AG LV V+ +Y QR +P R+R D L +A+ Sbjct: 240 SMWEVGAAIEAGELVTVLDKFAAPAPSIYAVFAQRRHLPLRIRAFVDFLRHAYAQ 294 >gi|300311929|ref|YP_003776021.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 304 Score = 125 bits (314), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 82/263 (31%), Positives = 131/263 (49%), Gaps = 11/263 (4%) Query: 4 RLEMLRIFCAAADTGSFKDAAIRLGISPQAVTRAIQELERLQGEMLFHRNTRQVRITSFG 63 +L + +FC AA+ GSF AA LG++P +V+R+I LE G LF R TRQ+R+T G Sbjct: 8 QLGSIELFCKAAELGSFTAAAEALGLTPASVSRSISRLESRLGVRLFARTTRQIRLTREG 67 Query: 64 EALAERARASLR---DLDGLFETDTASPADEMSGLVRLAAPSSLGRSRLLPIVERLAQAH 120 E ++ + +L D + + P SGL+R++ + RLLP++ R QA+ Sbjct: 68 ELYRDQCQQALEQIADAERILTGQQKVP----SGLLRISVGTPYAHYRLLPLLPRFQQAY 123 Query: 121 PGLRFDLRLTDQRADVVGEKIDIGIRVGTVPDNRFVVRRLGKVRFHVVATPRFLRRHGTP 180 P + +L + ++ D V E D+ IR+G PD+R + L + + A P +L R G P Sbjct: 124 PQIEVELSIANRVVDFVEEGYDLAIRLGVPPDSRLIAHTLEEATLGLFAAPAYLARRGQP 183 Query: 181 EDTEALRALPCTAMLDRGTGRPWPWLFAQ-ENQWMADHARF---LCDDSEGEYLLVRSGV 236 L C + +GR PW+F + + M H + + DD G +G Sbjct: 184 RSIAELEQHDCIQFILPSSGRAMPWIFKDAQGRDMDFHFKSRQRIHDDVLGCVNWAIAGG 243 Query: 237 AIGQIAGFLCEDDIAAGRLVPVM 259 + QI F+ + + G LV V+ Sbjct: 244 GLFQIYHFIADAAVRRGELVEVL 266 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 947,143 Number of extensions: 41285 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 228 Number of HSP's successfully gapped: 45 Length of query: 296 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 211 Effective length of database: 1,217,395 Effective search space: 256870345 Effective search space used: 256870345 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)