BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0200.p (286 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 496 e-141 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 125 4e-30 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 117 1e-27 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 106 2e-24 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 104 8e-24 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 496 bits (1276), Expect = e-141, Method: Compositional matrix adjust. Identities = 248/248 (100%), Positives = 248/248 (100%) Query: 39 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIA 98 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIA Sbjct: 1 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIA 60 Query: 99 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 158 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL Sbjct: 61 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVL 120 Query: 159 YGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEAPDGVR 218 YGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEAPDGVR Sbjct: 121 YGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEAPDGVR 180 Query: 219 TTIISPGAVDSELKTHSSDKETSEALLAWYEANQIPSDSVARAIAYAIEQPANVDISEVV 278 TTIISPGAVDSELKTHSSDKETSEALLAWYEANQIPSDSVARAIAYAIEQPANVDISEVV Sbjct: 181 TTIISPGAVDSELKTHSSDKETSEALLAWYEANQIPSDSVARAIAYAIEQPANVDISEVV 240 Query: 279 LRPVAQEF 286 LRPVAQEF Sbjct: 241 LRPVAQEF 248 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 125 bits (314), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 85/243 (34%), Positives = 127/243 (52%), Gaps = 6/243 (2%) Query: 47 VVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVTTDVARR 106 VV+ITGA++G G AR A G K++ RR ERL+ LV ++ A + + + DV + Sbjct: 2 VVLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGAD--KVLPLVLDVTSK 59 Query: 107 DDVEALVAKGEQHFGRIDVLVNNAGI-MPLAPMAKLKVEEWDRMIDVNVKGVLYGVAAAL 165 +E +A + +DVL+NNAG+ + L P ++ + +W+ MID NVKG++ A L Sbjct: 60 SSIETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALL 119 Query: 166 PRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTE-APDGVRTTIISP 224 P + G IIN+ SVAG + G G VY ATK V T LR + GVR T ++P Sbjct: 120 PDMVKRGRGTIINLGSVAGAYPYPG-GNVYGATKAFVDQFTLNLRADLVGTGVRATNLAP 178 Query: 225 GAVDSELKTHSSDKETSEALLAWYEANQ-IPSDSVARAIAYAIEQPANVDISEVVLRPVA 283 G ++ K A YE + +D +A A + PA+V+I+ + L P Sbjct: 179 GLCGGTEFSNVRFKGDDGAAAKVYEGTTPLTADDIAEAAFWVATMPAHVNINYMELMPTC 238 Query: 284 QEF 286 Q F Sbjct: 239 QGF 241 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 117 bits (293), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 70/196 (35%), Positives = 116/196 (59%), Gaps = 9/196 (4%) Query: 39 MSNNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIA 98 M+ +DKVVV+TG S G+G TA+ A+ GA++ + RR ++LE+ V +I G A+ Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREI---GNGAVG 59 Query: 99 VTTDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPL--APMAKLKVEEWDRMIDVNVKG 156 V D A+ DD++ L A+ + GR+DVL NAG P+ + E++D + ++NV+G Sbjct: 60 VPGDAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRG 119 Query: 157 VLYGVAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTVYSATKFAVRALTEGLRTEAPD- 215 +L+ V ALP + G I+ SVAG+K F G ++Y+A+K AVR+ + + Sbjct: 120 LLFSVQKALPLMS--QGGAIVLNGSVAGVKGFPGQ-SLYNASKAAVRSFARSWTADLKER 176 Query: 216 GVRTTIISPGAVDSEL 231 G+R +++PG ++ L Sbjct: 177 GIRVNVVAPGGTETRL 192 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 106 bits (265), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 74/225 (32%), Positives = 112/225 (49%), Gaps = 5/225 (2%) Query: 41 NNIKDKVVVITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVT 100 N ++ KV +ITGAS+G+G TA A GA L+L ARR + LE++ A I A GG+A V Sbjct: 2 NTLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVVA 61 Query: 101 TDVARRDDVEALVAKGEQHFGRIDVLVNNAG-IMPLAPMAKLKVEEWDRMIDVNVKGVLY 159 DV E VA FG +D+ +NNAG + P+A++ ++W + N+ Sbjct: 62 GDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFL 121 Query: 160 GVAAALPRFAAQSSGHIINVSSVAGIKV-FAGMGTVYSATKFAVRALTEGLRTE-APDGV 217 G +P A+ +G I+ SS G GM Y A K + L +G+ + A G+ Sbjct: 122 GARCQIPAMLARGAGAIVFTSSFVGSSAGLPGM-AAYGAAKAGLMGLVKGIAADYAVQGI 180 Query: 218 RTTIISPGAVDSELKTHSSDKETSEALLAWYEANQIPSDSVARAI 262 R + PG VD+++ K+ + L A Q P + A A+ Sbjct: 181 RANALLPGGVDTDMGGDPQQKQWAAGLHAMKRIAQ-PEEIAAAAL 224 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 104 bits (260), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 72/193 (37%), Positives = 111/193 (57%), Gaps = 7/193 (3%) Query: 43 IKDKVVVITGAS--SGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVT 100 +KDKV +ITG + +GLG +TAR +A+ GA++V+ ER E A G Q + + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVI--LDLERAEPAGA-AARLGAQHLGLV 59 Query: 101 TDVARRDDVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVLYG 160 DV + EA A + FGRID+LVNNAG+ A + ++ +++DR++DV+++G LY Sbjct: 60 ADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYM 119 Query: 161 VAAALPRFAAQSSGHIINVSSVAGIKVFAGMGTV-YSATKFAVRALTEGLRTE-APDGVR 218 A LP AQ SG I+ +SSV+ + +G YSA K V L + E P G+R Sbjct: 120 SQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGIR 179 Query: 219 TTIISPGAVDSEL 231 I+PG + +++ Sbjct: 180 VNCITPGLISTDI 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 862,193 Number of extensions: 34877 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 48 Length of query: 286 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 202 Effective length of database: 1,222,130 Effective search space: 246870260 Effective search space used: 246870260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)