BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0198.p (280 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 556 e-160 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 100 1e-22 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 87 2e-18 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 84 1e-17 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 77 1e-15 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 556 bits (1434), Expect = e-160, Method: Compositional matrix adjust. Identities = 274/280 (97%), Positives = 277/280 (98%) Query: 1 MQSIPLLSLRNGQSVPILGQGTWNMGESPAQASAEVRALQAGLDLGMRLIDTAEMYAEGG 60 MQSIPLLSLRNGQSVPILGQGTWNMGESPAQA+AEVRALQAGLDLGM LIDTAEMYAEGG Sbjct: 1 MQSIPLLSLRNGQSVPILGQGTWNMGESPAQANAEVRALQAGLDLGMSLIDTAEMYAEGG 60 Query: 61 AERIVAKAIAGRREQVYLVSKVLPHNASKRGTIAACEASLKRLGTDRIDLYLLHWRGAHP 120 AERIVAKAIAGRR+QVYLVSKVLPHNASKRGTIAACEASLKRLGTDRIDLYLLHWRGAHP Sbjct: 61 AERIVAKAIAGRRDQVYLVSKVLPHNASKRGTIAACEASLKRLGTDRIDLYLLHWRGAHP 120 Query: 121 LAATIEGMEALVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDL 180 LAATIEGME LVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDL Sbjct: 121 LAATIEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDL 180 Query: 181 LPQAQQAHIAVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAA 240 LPQAQQAHIAVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAA Sbjct: 181 LPQAQQAHIAVMAYSPIEQGRILKHPALTALAQAHRVTPAQIALAWVLRQPGVVAIPKAA 240 Query: 241 DVAHVQANRASLDISLNAADLKALDEAFAPPRRKQPLAML 280 DVAHVQANRASLDISL+AADLK LDEAFAPPRRKQPLAML Sbjct: 241 DVAHVQANRASLDISLSAADLKILDEAFAPPRRKQPLAML 280 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 100 bits (250), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 91/305 (29%), Positives = 135/305 (44%), Gaps = 51/305 (16%) Query: 11 NGQSVPILGQGTWNMGESPAQA--SAEVRALQAGLDLGMRLIDTAEMYAEGGAERIVAKA 68 NG V +G G M + AQ + + + L+LG+ L+DTA+MY E ++ +A Sbjct: 23 NGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEELLGRA 82 Query: 69 IAGRREQVYLVSKV----LPHNASKRGTIA-------ACEASLKRLGTDRIDLYLLH-WR 116 I GRR++ ++ +K P + RG A E SLKRLG + IDLY H Sbjct: 83 IKGRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDLYYQHRID 142 Query: 117 GAHPLAATIEGMEALVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCN-QVLYNLSRRG 175 A P+ T+ + LV GKI G+S LE H H + Q Y+L R Sbjct: 143 PATPIEVTMGVLADLVQAGKIRYIGLSEASA----ATLERAHRVHPVTALQSEYSLWTRD 198 Query: 176 IEYDLLPQAQQAHIAVMAYSPIEQGRIL----------------KHPALTA--------- 210 E ++L + I +AYSP+ +G + +P Sbjct: 199 PEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSNPRFQGENFARNLQL 258 Query: 211 ------LAQAHRVTPAQIALAWVLRQ-PGVVAIPKAADVAHVQANRASLDISLNAADLKA 263 +A H TP+Q+ALAWV+ Q P +V IP +++ N S+ + L DL+A Sbjct: 259 AEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQA 318 Query: 264 LDEAF 268 LD F Sbjct: 319 LDAIF 323 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 86.7 bits (213), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 98/352 (27%), Positives = 145/352 (41%), Gaps = 83/352 (23%) Query: 1 MQSIPLLSLRNGQSVPILGQGTWNMGESPAQASAEVRALQAGLDLGMRLIDTAEMY---- 56 MQ PL S+ LG TW S A A A+ L ++ G+ LID AEMY Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQ---LDYAVERGINLIDVAEMYPVPP 57 Query: 57 ---AEGGAERIVAKAI--AGRREQVYLVSKVL-----PHNA----------SKRGTIAAC 96 +G ER + + +G+R+Q+ + +K PHN ++G A Sbjct: 58 KAETQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEAL 117 Query: 97 EASLKRLGTDRIDLYLLHW------------------RGAHPLAATIEGMEALVSQGKIG 138 SL+RL D +DLY LHW P+ T+ + V GKI Sbjct: 118 HGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIR 177 Query: 139 GWGVSNFDTDDIDDLLE--EPHG-EHYLCNQVLYNLSRRGIEYDLLPQAQQAHIAVMAYS 195 G+SN + L E HG E + Q YNL R E +L A + + ++AYS Sbjct: 178 TIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYS 237 Query: 196 PIE----QGRILK--HPA------------------------LTALAQAHRVTPAQIALA 225 P+ G+ L PA ALA+ H + PAQ+ALA Sbjct: 238 PLAFGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALA 297 Query: 226 WVLRQPGVVA-IPKAADVAHVQANRASLDISLNAADLKALDEAFAPPRRKQP 276 +V +P + + I A +A +++N S ++ L L A++ R+QP Sbjct: 298 YVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAI----HRRQP 345 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 86/295 (29%), Positives = 137/295 (46%), Gaps = 56/295 (18%) Query: 10 RNGQSVPILGQGTWNMGESPAQASAE--VRALQAGLDLGMRLIDTAEMYAEGGAERIVAK 67 R G V +G G W +G S SA+ AL A LD G+ IDTA++Y +G +E+++A+ Sbjct: 8 RTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSEQLIAQ 67 Query: 68 AIAGR-REQVYLVSK----VLPHNAS---KRGTIAA-CEASLKRLGTDRIDLYLLHW--R 116 + R E+ ++ +K + PH AS R ++A + SLK LG D +DL LH Sbjct: 68 VLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLVQLHCPPT 127 Query: 117 GAHPLAATIEGMEALVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGI 176 + ++ALV+ GKI +GVS ++ +E P+ + Q+++N+ R+ Sbjct: 128 EVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSV---QIIFNIFRQRP 184 Query: 177 EYDLLPQAQQAHIAVMAYSPIEQG------------------RILKH------------- 205 L +AQ+ IAV+A P+ G + +H Sbjct: 185 ISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGV 244 Query: 206 PALTALAQAHRVTP--------AQIALAWVLRQPGV-VAIPKAADVAHVQANRAS 251 P ALA ++ AQ AL W+L + G+ IP A +VA Q+N A+ Sbjct: 245 PYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAA 299 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 77.4 bits (189), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 58/176 (32%), Positives = 89/176 (50%), Gaps = 12/176 (6%) Query: 37 RALQAGLDLGMRLIDTAEMYAEGGAERIVAKAIAGRREQVYLVSKV------LPH--NAS 88 R + + + G+ IDTA++Y +G +E IV K +AG+R + L SK+ P+ N S Sbjct: 34 RIVASAREHGVNFIDTADVYTKGKSEEIVGKLLAGQRHEWVLASKLGNAMSGKPNESNYS 93 Query: 89 KRGTIAACEASLKRLGTDRIDLYLLHW-RGAHPLAATIEGMEALVSQGKIGGWGVSNF-- 145 + + E L RL TD +D+ +H L ++ + L+ GKI G+GVSNF Sbjct: 94 RSWIVRETENILTRLDTDYLDILYMHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRG 153 Query: 146 -DTDDIDDLLEEPHGEHYLCNQVLYNLSRRGIEYDLLPQAQQAHIAVMAYSPIEQG 200 +I L E+ + L Q YNL R E ++LP + V+ YSPI +G Sbjct: 154 WRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARG 209 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 914,721 Number of extensions: 39396 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 6 Length of query: 280 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 196 Effective length of database: 1,222,130 Effective search space: 239537480 Effective search space used: 239537480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)