BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0194.p (272 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309645|ref|YP_003773737.1| transketolase N-terminal subuni... 554 e-159 gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [He... 144 7e-36 gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum s... 119 3e-28 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 114 1e-26 >gi|300309645|ref|YP_003773737.1| transketolase N-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 272 Score = 554 bits (1427), Expect = e-159, Method: Compositional matrix adjust. Identities = 270/272 (99%), Positives = 271/272 (99%) Query: 1 MKNDFVDLRAKAHWVWCETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA 60 MKNDFVDLRAKAHWVW ETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA Sbjct: 1 MKNDFVDLRAKAHWVWRETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA 60 Query: 61 GRDRCVISKGHGSICMYPILADLGYFPMEELQHVCEAGSFLGGIPDPVIPGYETVNGSLG 120 GRDRCVISKGHGSICMYPILADLGYFPMEELQHVCEAGSFLGGIPDPVIPGYETVNGSLG Sbjct: 61 GRDRCVISKGHGSICMYPILADLGYFPMEELQHVCEAGSFLGGIPDPVIPGYETVNGSLG 120 Query: 121 HGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDNRI 180 HGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDNRI Sbjct: 121 HGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDNRI 180 Query: 181 SMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVLSVQAALLQQKAHAAGKPKAIIARTL 240 SMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVL+VQAALLQQKAHAAGKPKAIIARTL Sbjct: 181 SMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVLAVQAALLQQKAHAAGKPKAIIARTL 240 Query: 241 KGHGVPGLENAPLSHIINPKPELIDDLLEKNR 272 KGHGVPGLENAPLSHIINPKPELIDDLLEKNR Sbjct: 241 KGHGVPGLENAPLSHIINPKPELIDDLLEKNR 272 >gi|300310439|ref|YP_003774531.1| transketolase alpha subunit [Herbaspirillum seropedicae SmR1] Length = 281 Score = 144 bits (364), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 92/263 (34%), Positives = 134/263 (50%), Gaps = 4/263 (1%) Query: 1 MKNDFVDLRAKAHWVWCETLAIHRRAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDA 60 M ++ +LRA A+ + + + + + +L D+ +A+ Y + P DP+ + Sbjct: 1 MSDNLANLRASAYRIRRNAVRMGEVQGQGYIGQALGWADV-LAVAYKHAMSLRPDDPQWS 59 Query: 61 GRDRCVISKGHGSICMYPILADLGYFPMEELQHVCEAGSFL--GGIPDPVIPGYETVNGS 118 GRDR ++S GH +I +Y L + G P EEL S L G+ PG E GS Sbjct: 60 GRDRFLLSHGHYAIALYAALIEAGIVPDEELDSYGSDDSRLPMSGMA-TYTPGMEISGGS 118 Query: 119 LGHGLGVATGMALGLKRSGSDRSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDN 178 LG GL + GMALGL+ + + V+ DGEL EG+ WEA M A+ H+L NL +VD N Sbjct: 119 LGQGLAIGVGMALGLRYNNNPAFVYNSMSDGELDEGSTWEAAMGAAHHQLGNLICLVDIN 178 Query: 179 RISMLGYTEDIVSHGELATRLSAFGWDCMEVDGHDVLSVQAALLQQKAHAAGKPKAIIAR 238 G + I+ LA + +AFGW VDG+D+ +V A + KP+ I+ Sbjct: 179 NQQADGPSGKIMGFEPLADKWAAFGWYVQRVDGNDLAAVVQAFDNARNLKDAKPRVILFD 238 Query: 239 TLKGHGVPGLENAPLSHIINPKP 261 TL G GVP LE +H I P Sbjct: 239 TLMGKGVPFLEQREKNHFIRVDP 261 >gi|300309841|ref|YP_003773933.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 119 bits (298), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 89/236 (37%), Positives = 118/236 (50%), Gaps = 16/236 (6%) Query: 25 RAPETRLASSLSSIDIFVALYYGGVLRFNPADPRDAGRDRCVISKGHGSICMYPILADLG 84 RA E L +I ALY LRFNPA P RDR V+S GHGS+ +Y +L G Sbjct: 23 RATEGHQGVPLGMAEIATALY-ARHLRFNPAAPTWWDRDRVVLSNGHGSMLLYCLLYLTG 81 Query: 85 Y--FPMEELQHVCEAGSFLGGIPD--PVIPGYETVNGSLGHGLGVATGMALGL----KRS 136 Y +E+++ E GS G P+ P + G E G LG G+ A GMA+ R Sbjct: 82 YEHITLEQVRSFREFGSHCAGHPEYAPEL-GIEVTTGPLGQGIANAFGMAVAEAYLNARF 140 Query: 137 GSD---RSVFVVAGDGELHEGANWEAIMFASQHKLDNLHLIVDDNRISMLGYTEDIVSHG 193 G D + GDG L EG E I A +L L L+ DDN+I+ G TE +S Sbjct: 141 GDDIVNHRTYAFVGDGCLQEGIGQEMISLAGHLRLHKLTLLWDDNQITDDGKTELSISE- 199 Query: 194 ELATRLSAFGWDCMEVDGHDVLSVQAALLQQKAHAAGKPKAIIARTLKGHGVPGLE 249 +A R GW +EVDGHD+ +V AL A + +P I RT+ G G+P ++ Sbjct: 200 NVAERFRVAGWHVVEVDGHDIQAVSRAL--DAAQGSDRPSMIACRTVIGRGIPRVQ 253 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 114 bits (284), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 85/223 (38%), Positives = 112/223 (50%), Gaps = 17/223 (7%) Query: 39 DIFVALYYGGVLRFNPADPRDAGRDRCVISKGHGSICMYPILADLGY-FPMEELQHVCEA 97 +I VAL+ R NPA+P A RDR V+S GHGS+ Y +L GY ME+++ + Sbjct: 38 EIAVALW-AKHYRHNPANPHWANRDRFVLSNGHGSMLQYALLHLTGYDLSMEDIKSFRQL 96 Query: 98 GSFLGGIPDP-VIPGYETVNGSLGHGLGVATGMALGLKRSGS----------DRSVFVVA 146 S G P+ + PG ET G LG G+ A GMAL K + D + Sbjct: 97 HSKTAGHPEVHITPGVETTTGPLGQGITNAVGMALAEKLLAAEFNKPGLAVVDHYTYAFV 156 Query: 147 GDGELHEGANWEAIMFASQHKLDNLHLIVDDNRISMLGYTEDIVSHGELATRLSAFGWDC 206 GDG L EG + EA A L L ++ DDN IS+ G+ E + R A+GW+ Sbjct: 157 GDGCLMEGISHEACSLAGVLNLSKLIVLYDDNGISIDGHVEGWFKD-DTPKRFEAYGWNV 215 Query: 207 M-EVDGHDVLSVQAALLQQKAHAAGKPKAIIARTLKGHGVPGL 248 + VDGH V +V AA+ Q K A KP I RT+ G G P L Sbjct: 216 IPAVDGHSVEAVSAAIHQAK--LADKPTLICCRTVIGKGSPNL 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 923,837 Number of extensions: 40642 Number of successful extensions: 129 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 4 Length of query: 272 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 188 Effective length of database: 1,222,130 Effective search space: 229760440 Effective search space used: 229760440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)