BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0193.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309646|ref|YP_003773738.1| transketolase C-terminal subuni... 635 0.0 gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subun... 88 8e-19 gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum s... 76 3e-15 gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate ... 70 2e-13 >gi|300309646|ref|YP_003773738.1| transketolase C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 321 Score = 635 bits (1638), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/316 (98%), Positives = 313/316 (99%) Query: 1 MAMRDALLQRIWSAMAEDRQIFFTCADFGSPVLDKIRADFPDRFVNVGIAEQNLINVSAG 60 MAMRDALLQRIWSAMAEDRQIFFTCADFGSPVLDKIRADFPDRFVNVGIAEQNLINVSAG Sbjct: 6 MAMRDALLQRIWSAMAEDRQIFFTCADFGSPVLDKIRADFPDRFVNVGIAEQNLINVSAG 65 Query: 61 LALEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPMNVNLIGVGAGYSYVVSGPTHQC 120 LALEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPMNVNLIGVGAGYSYVVSGPTHQC Sbjct: 66 LALEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPMNVNLIGVGAGYSYVVSGPTHQC 125 Query: 121 YEDITLMRALPNFQVLSAADHVTAAAFFDTCSNSTGPKYLRFDAQVLPVIYGDGAPDLAQ 180 YEDITLMRALPNFQVLSAADHVTAAAFFDTCSNS GPKYLRFDAQVLPVIYGDGAPDLAQ Sbjct: 126 YEDITLMRALPNFQVLSAADHVTAAAFFDTCSNSMGPKYLRFDAQVLPVIYGDGAPDLAQ 185 Query: 181 GFHIHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEVGVIDLFDLARFSADALQETLQTY 240 GFHIHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEVGVIDLFDLARFSADALQETLQ+Y Sbjct: 186 GFHIHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEVGVIDLFDLARFSADALQETLQSY 245 Query: 241 AGIATLEEGFRGRGGMDSMLFEFIARRGLPTRMLNIGVDGVYRFELGTRTELHEEVGIGP 300 AGIATLEEGFRGRGGMDSMLFEFIARRGL TRMLNIGVDGVYRFELGTRTELHEEVGIGP Sbjct: 246 AGIATLEEGFRGRGGMDSMLFEFIARRGLQTRMLNIGVDGVYRFELGTRTELHEEVGIGP 305 Query: 301 QAVLTSIREFATSISN 316 QAVLTSIREFA SI++ Sbjct: 306 QAVLTSIREFAASIAS 321 >gi|300310438|ref|YP_003774530.1| transketolase, C-terminal subunit [Herbaspirillum seropedicae SmR1] Length = 334 Score = 88.2 bits (217), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 69/274 (25%), Positives = 125/274 (45%), Gaps = 10/274 (3%) Query: 40 FPDRFVNVGIAEQNLINVSAGLALEGYKVFAYAIAPFITMRCYEQVRVSLALLSEVRPMN 99 +P+RF +G+AEQ L+ + G+A EG FA A F T R Y+ + +A + + Sbjct: 66 YPERFFQMGMAEQLLMAAAGGMAKEGLVPFATTYAVFATRRAYDFIHQVIAEENLNVKIC 125 Query: 100 VNLIGVGAGYSYVVSGPTHQCYEDITLMRALPNFQVLSAADHVTAAAFFDTCSNSTGPKY 159 L G+ GY GP+HQ ED+ +MRA+P ++ D + + GP Y Sbjct: 126 CALPGLTTGY-----GPSHQATEDLAMMRAIPGMTIVDPCDALDLEQAVPQIAAHQGPVY 180 Query: 160 LRFDAQVLPVIYG--DGAPDLAQGFHIHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEV 217 +R +P++ D +L + I R G + ++++G+M AL+ A+ L A+ + V Sbjct: 181 MRLARGNVPLVLDEYDYRFELGRAKEI-RDGNDVLIISSGFMTMRALEVAKALEADKVSV 239 Query: 218 GVIDLFDLARFSADALQETLQTYAGIATLEEGFRGRGGMDSMLFEFIAR-RGLPTRMLNI 276 V+ + F + ++ + + E GG+ ++ + R R P Sbjct: 240 AVLHCPTIKPFDEATVVAQVKKKNRLVVVAENHSVVGGLGEIVATTLMRHRVQPAGFQLA 299 Query: 277 GVDGVYRFELGTRTELHEEVGIGPQAVLTSIREF 310 G+ + + G LH+ GI + + I+E+ Sbjct: 300 GLPDAF-LDAGALPTLHDRYGISCEVLGRRIKEW 332 >gi|300310747|ref|YP_003774839.1| transketolase [Herbaspirillum seropedicae SmR1] Length = 664 Score = 76.3 bits (186), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 66/203 (32%), Positives = 99/203 (48%), Gaps = 23/203 (11%) Query: 36 IRADFPDRFVNVGIAEQNLINVSAGLALEG-YKVFAYAIAPFITMRCYEQVRVSLALLSE 94 +RAD +N G+ E + + G+AL G Y F A F+T Y + + +A L + Sbjct: 398 VRADQRGNHINYGVREFGMSAIMNGIALHGGYLPFG---ATFLTFSDYSRNALRMAALMK 454 Query: 95 VRPMNV---NLIGVGAGYSYVVSGPTHQCYEDITLMRALPNFQVLSAADHV-TAAAFFDT 150 +R + V + IG+G GPTHQ E ++ +R +PN D TA A+ ++ Sbjct: 455 IRALFVFTHDSIGLGE------DGPTHQSVEHVSSLRLIPNLDNWRPCDTTETAVAWAES 508 Query: 151 CSNSTGPKYLRFDAQVLPVIYGDGA--PDLAQGFHIHRRG--GKIGLVATGYMLHTALQA 206 GP L F Q LP A D+A+G ++ R K+ L+ATG + A +A Sbjct: 509 IKRHDGPSTLIFSRQNLPFQERTDAQVKDIARGGYVLRDAPNAKVILMATGSEIELATKA 568 Query: 207 AEVLAAEGIEVGVI-----DLFD 224 A+ L AEGI V V+ D+FD Sbjct: 569 ADALTAEGIAVRVVSMPSTDVFD 591 >gi|300313125|ref|YP_003777217.1| 1-deoxy-D-xylulose 5-phosphate synthase [Herbaspirillum seropedicae SmR1] Length = 621 Score = 70.5 bits (171), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 81/306 (26%), Positives = 142/306 (46%), Gaps = 31/306 (10%) Query: 14 AMAEDRQIFFTCA-DFGSPVLDKIRADFPDRFVNVGIAEQNLINVSAGLALEGYKVFAYA 72 A A+D+ + T A GS ++D FP+R+ +VGIAEQ+ + +AG+A EG K Sbjct: 323 AAADDKLVGITPAMREGSGMVD-FEKKFPNRYYDVGIAEQHAVTFAAGMACEGMKPVVAI 381 Query: 73 IAPFITMRCYEQVRVSLALLSEVRPMNVNLIG-VGAGYSYVVSGPTHQCYEDITLMRALP 131 + F+ R Y+Q+ +AL + ++ G VGA G TH D+ +R +P Sbjct: 382 YSTFL-QRAYDQLIHDVALQNLDVTFALDRAGLVGA------DGATHAGNYDLAFLRCIP 434 Query: 132 NFQVLSAADHVTAAAFFDTCSNSTGPKYLRFDAQVLPVIYGDGAP--------DLAQGFH 183 N V++A+D T GP +R+ P G GA L +G Sbjct: 435 NMVVMAASDENECRKMLSTAYQYPGPASVRY-----PRGAGIGAAVDQTLETLPLGRG-E 488 Query: 184 IHRRGGKIGLVATGYMLHTALQAAEVLAAEGIEVGVIDLFDLARFSADALQETLQTYAGI 243 I R+G + ++A G +L +L A + L A + I D+ + +++ ++ + Sbjct: 489 IRRQGKNVAILAFGSLLAPSLGAGDKLNATVANMRFIKPLDV-----ELVKQLAASHDAL 543 Query: 244 ATLEEGFRGRGGMDSMLFEFIARRGLPTRMLNIGVDGVYRFELGTRTELHEEVGIGPQAV 303 T+EEG GG + + E +A G+ +L++G+ + + G +L + G+ + Sbjct: 544 VTVEEGCI-MGGAGAAVSEALAAAGINKPILHLGLPDRF-IDHGDAAQLLAQCGLDANGI 601 Query: 304 LTSIRE 309 SI + Sbjct: 602 ADSIAQ 607 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 938,261 Number of extensions: 39771 Number of successful extensions: 167 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 4 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)