BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0190.p (312 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309647|ref|YP_003773739.1| nucleoside-diphosphate-sugar ep... 649 0.0 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 62 8e-11 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 61 1e-10 >gi|300309647|ref|YP_003773739.1| nucleoside-diphosphate-sugar epimerase [Herbaspirillum seropedicae SmR1] Length = 312 Score = 649 bits (1675), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/312 (100%), Positives = 312/312 (100%) Query: 1 MSSKILVTGGAGYIGSMLVPELLKLGHEVTVVDNFMFRQASLNHCCGYANFNVVNGDIRH 60 MSSKILVTGGAGYIGSMLVPELLKLGHEVTVVDNFMFRQASLNHCCGYANFNVVNGDIRH Sbjct: 1 MSSKILVTGGAGYIGSMLVPELLKLGHEVTVVDNFMFRQASLNHCCGYANFNVVNGDIRH 60 Query: 61 EDFIRPHYEAAEIIIPLAAYVGAPLCNKDKTGAQTVNHDAIMAMLGMLRDDQKVLMPTTN 120 EDFIRPHYEAAEIIIPLAAYVGAPLCNKDKTGAQTVNHDAIMAMLGMLRDDQKVLMPTTN Sbjct: 61 EDFIRPHYEAAEIIIPLAAYVGAPLCNKDKTGAQTVNHDAIMAMLGMLRDDQKVLMPTTN 120 Query: 121 SAYGSGDKDNFCDENSPLNPISKYAIDKVAIEKELMKRQNSISFRLATVFGMAPKMRIDL 180 SAYGSGDKDNFCDENSPLNPISKYAIDKVAIEKELMKRQNSISFRLATVFGMAPKMRIDL Sbjct: 121 SAYGSGDKDNFCDENSPLNPISKYAIDKVAIEKELMKRQNSISFRLATVFGMAPKMRIDL 180 Query: 181 LVNDFVYRAVYDRFIVLFEGHFKRNYIHVTDVVRAFLHGIDNFDTMRGEIFNVGLSEANV 240 LVNDFVYRAVYDRFIVLFEGHFKRNYIHVTDVVRAFLHGIDNFDTMRGEIFNVGLSEANV Sbjct: 181 LVNDFVYRAVYDRFIVLFEGHFKRNYIHVTDVVRAFLHGIDNFDTMRGEIFNVGLSEANV 240 Query: 241 SKAELCAWIKKHIPDLVVMEAPLATDPDQRNYVVSNAKLEATGWKPQVGLDAGIRELIKG 300 SKAELCAWIKKHIPDLVVMEAPLATDPDQRNYVVSNAKLEATGWKPQVGLDAGIRELIKG Sbjct: 241 SKAELCAWIKKHIPDLVVMEAPLATDPDQRNYVVSNAKLEATGWKPQVGLDAGIRELIKG 300 Query: 301 YAMIKNTRYGNV 312 YAMIKNTRYGNV Sbjct: 301 YAMIKNTRYGNV 312 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 61.6 bits (148), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 66/250 (26%), Positives = 105/250 (42%), Gaps = 16/250 (6%) Query: 4 KILVTGGAGYIGSMLVPELLKLGHEVTVVDNFMF-RQASLNH------CCGYANFNVVNG 56 + L+TG AG+IGS L+ LL LG +VT +DNF RQA+L +ANF + G Sbjct: 17 RWLITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIEG 76 Query: 57 DIRHEDFIRPHYEAAEIIIPLAAYVGAPLCNKDKTGAQTVNHDAIMAMLGMLRDDQKVLM 116 DI + A+ ++ AA +D N + + ML RD Sbjct: 77 DICDAATCLRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVARF 136 Query: 117 PTTNSAYGSGDKDNFCD-ENSPLNPISKYAIDKVAIE--KELMKR---QNSISFRLATVF 170 S+ GD E P+S YA+ K E E+ R SI R VF Sbjct: 137 VYAASSSTYGDHPGLPKVEEVIGKPLSPYAVTKYVNELYAEVFARCYGMESIGLRYFNVF 196 Query: 171 G--MAPKMRIDLLVNDFVYRAVYD-RFIVLFEGHFKRNYIHVTDVVRAFLHGIDNFDTMR 227 G P ++ +V + + + +G R++ ++ +VV+A L + Sbjct: 197 GRRQDPHGAYAAVIPQWVTAMIRNGELRINGDGQTSRDFCYIDNVVQANLLAATAGEEAV 256 Query: 228 GEIFNVGLSE 237 +++NV L++ Sbjct: 257 NQVYNVALND 266 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 74/274 (27%), Positives = 121/274 (44%), Gaps = 29/274 (10%) Query: 6 LVTGGAGYIGSMLVPELLKLGHEVTVVDNFMFR-QASLNHCCG------YANFNVVNGDI 58 LVTG AG+IGS L+ LLKL V +DNF Q +L+ +ANF V GDI Sbjct: 20 LVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQWANFRFVEGDI 79 Query: 59 RHEDFIRP--HYEA-----AEIIIPLAAYVGAPLCNKDKTGAQTVNHDAIMAMLGMLRDD 111 R D R H+EA E ++ AA P +D N D + ML RD Sbjct: 80 RKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLVAARDA 139 Query: 112 --QKVLMPTTNSAYGSGDKDNFCD-ENSPLNPISKYAIDKVAIE--KELMKRQ---NSIS 163 ++ + ++S Y GD E+ P+S YA+ K E ++ R +I Sbjct: 140 RVKRFVYAASSSTY--GDHPGLPKVEDRIGKPLSPYAVTKFVNELYADVFARTYGLQTIG 197 Query: 164 FRLATVFG--MAPKMRIDLLVNDFVYRAVY-DRFIVLFEGHFKRNYIHVTDVVRA-FLHG 219 R +FG P ++ ++ A+ ++ + +G R++ +V + V+A L Sbjct: 198 LRYFNIFGRRQNPDGAYAAVIPKWIAAAIKGEQIYINGDGETSRDFCYVDNAVQANLLAA 257 Query: 220 IDNFDTMRGEIFNVGLSEANVSKAELCAWIKKHI 253 D +++NV + + K EL +++ ++ Sbjct: 258 CTLQDEAANQVYNVAVGDRTTLK-ELYQFLRDNL 290 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 938,134 Number of extensions: 36608 Number of successful extensions: 140 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 3 Length of query: 312 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 227 Effective length of database: 1,217,395 Effective search space: 276348665 Effective search space used: 276348665 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)