BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0181.p (637 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase sub... 355 9e-99 gi|300309454|ref|YP_003773546.1| GTPase translation elongation f... 69 1e-12 gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein... 69 1e-12 >gi|300312403|ref|YP_003776495.1| sulfate adenylyltransferase subunit 1 protein [Herbaspirillum seropedicae SmR1] Length = 443 Score = 355 bits (910), Expect = 9e-99, Method: Compositional matrix adjust. Identities = 188/424 (44%), Positives = 266/424 (62%), Gaps = 15/424 (3%) Query: 33 QQNLLRFITCGSVDDGKSTLIGRLLWESQQVFDDQFSTLQADSRRHGTQGQEIDFALLVD 92 ++ LLRFIT GSVDDGKSTLIGRLL++S+ +F DQ + + ++ T G +D +LL D Sbjct: 19 ERGLLRFITAGSVDDGKSTLIGRLLFDSKGIFADQLDAI-SRAKHKRTVGDTVDLSLLTD 77 Query: 93 GLAAERDKYHHTHVHGAPPATPHRRFIVADTPGHEQYTRNMITGASTADAAVLLVDARQ- 151 GL AER++ V ATP R+FI+ADTPGHEQYTRNM+TGASTADA ++LVD + Sbjct: 78 GLEAEREQGITIDVAYRYFATPKRKFIIADTPGHEQYTRNMVTGASTADAVIILVDVSKV 137 Query: 152 --------GVLTQTRRHAHLIALVGIRKVILAVNKMDLVAYDQEAFEAIVAEFHRLAKPL 203 +LTQT+RH+ + L+ I+ VI+AVNKMDLV YDQ ++ IV + + A+ L Sbjct: 138 KLGEDGSVELLTQTKRHSTIAHLLQIQHVIVAVNKMDLVDYDQTVYDRIVGAYQQFAQQL 197 Query: 204 GFDNITAIPLSALQGENITRRCARTPWYQGPTLMGCLETLNIDPPCQSQVV-FPVQWVNR 262 G ++ AIPLSAL G+N+ AR PWYQGPTL+ LE+L++ + FPVQ V R Sbjct: 198 GLQDVHAIPLSALAGDNVVEASARMPWYQGPTLITLLESLSVYEDAHEEAFRFPVQLVAR 257 Query: 263 PN----DSFRGFSGTLASGCLTVGDQLRVTSSGQTAALARIVTADGDLQSAQAGYAITVV 318 N + FRG+ G + +G + GD+L V SGQ+A + I T DG L+SA AG ++T++ Sbjct: 258 HNGHEANDFRGYMGRIEAGKVARGDKLVVLPSGQSATVKDIQTLDGSLESASAGQSVTIL 317 Query: 319 LDREVDASRGDIIGLADQPLEMSDQFEATLVWMHDEPGLMGRAYDMKLANQWASTTITSL 378 LD +D SRGD++ A QP + A + W+ +EP + R Y +K + + + + Sbjct: 318 LDEYLDISRGDLLASAAQPSTILKTVNADVCWLSEEPLDLRRKYWLKHTTKQVAARVAKV 377 Query: 379 KYRIDVNTQAHESCRQLVQNDIATINLALSRPLVFAPYEKNHTLGGFILVDKFTHATVAA 438 +D+NTQ L NDIA I++ + +P+ Y+ G FIL+D+ +H TVAA Sbjct: 378 DTLLDINTQERRPAETLKLNDIARISVNVQQPIAADAYQAIRATGAFILIDEVSHQTVAA 437 Query: 439 GMIR 442 GMIR Sbjct: 438 GMIR 441 >gi|300309454|ref|YP_003773546.1| GTPase translation elongation factor TU [Herbaspirillum seropedicae SmR1] Length = 396 Score = 69.3 bits (168), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 79/309 (25%), Positives = 128/309 (41%), Gaps = 48/309 (15%) Query: 41 TCGSVDDGKSTLIGRLLWESQQVFDDQFSTLQADSRRHGTQGQEIDFALLVDGLAAERDK 100 T G VD GK+TL + +T+ S++ G + + D +D E+ + Sbjct: 17 TIGHVDHGKTTLTAAI------------ATVL--SKKFGGEAKAYD---QIDAAPEEKAR 59 Query: 101 YHHTHVHGAPPATPHRRFIVADTPGHEQYTRNMITGASTADAAVLLVDARQGVLTQTRRH 160 + T +R + D PGH Y +NMITGA+ D A+L+ A G + QTR H Sbjct: 60 GITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH 119 Query: 161 AHLIALVGIRKVILAVNKMDLVAYDQEAFEAIVAEFHRLAKPLGF--DNITAIPLS---A 215 L VG+ +I+ +NK D+V D E E + E L F D++ + S A Sbjct: 120 ILLSRQVGVPYIIVFLNKADMVD-DAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLA 178 Query: 216 LQGENITRRCARTPWYQGP----TLMGCLETLNIDPPCQSQVV-----FPVQWVNRPNDS 266 L+G+ GP +M E L+ P + V PV+ V + Sbjct: 179 LEGDT------------GPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGR 226 Query: 267 FRGFSGTLASGCLTVGDQLRVT--SSGQTAALARIVTADGDLQSAQAGYAITVVL--DRE 322 +G + G + VG+++ + + Q + L QAG + V+L + Sbjct: 227 GTVVTGRVERGIIKVGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKR 286 Query: 323 VDASRGDII 331 D RG ++ Sbjct: 287 EDVQRGQVL 295 >gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 69.3 bits (168), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 79/309 (25%), Positives = 128/309 (41%), Gaps = 48/309 (15%) Query: 41 TCGSVDDGKSTLIGRLLWESQQVFDDQFSTLQADSRRHGTQGQEIDFALLVDGLAAERDK 100 T G VD GK+TL + +T+ S++ G + + D +D E+ + Sbjct: 17 TIGHVDHGKTTLTAAI------------ATVL--SKKFGGEAKAYD---QIDAAPEEKAR 59 Query: 101 YHHTHVHGAPPATPHRRFIVADTPGHEQYTRNMITGASTADAAVLLVDARQGVLTQTRRH 160 + T +R + D PGH Y +NMITGA+ D A+L+ A G + QTR H Sbjct: 60 GITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH 119 Query: 161 AHLIALVGIRKVILAVNKMDLVAYDQEAFEAIVAEFHRLAKPLGF--DNITAIPLS---A 215 L VG+ +I+ +NK D+V D E E + E L F D++ + S A Sbjct: 120 ILLSRQVGVPYIIVFLNKADMVD-DAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLA 178 Query: 216 LQGENITRRCARTPWYQGP----TLMGCLETLNIDPPCQSQVV-----FPVQWVNRPNDS 266 L+G+ GP +M E L+ P + V PV+ V + Sbjct: 179 LEGDT------------GPLGEQAIMALAEALDTYIPTPERAVDGTFLMPVEDVFSISGR 226 Query: 267 FRGFSGTLASGCLTVGDQLRVT--SSGQTAALARIVTADGDLQSAQAGYAITVVL--DRE 322 +G + G + VG+++ + + Q + L QAG + V+L + Sbjct: 227 GTVVTGRVERGIIKVGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKR 286 Query: 323 VDASRGDII 331 D RG ++ Sbjct: 287 EDVQRGQVL 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,919,331 Number of extensions: 80247 Number of successful extensions: 434 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 433 Number of HSP's successfully gapped: 3 Length of query: 637 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 546 Effective length of database: 1,188,985 Effective search space: 649185810 Effective search space used: 649185810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)