BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0178.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312398|ref|YP_003776490.1| permease [Herbaspirillum serope... 119 2e-28 >gi|300312398|ref|YP_003776490.1| permease [Herbaspirillum seropedicae SmR1] Length = 257 Score = 119 bits (299), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 87/236 (36%), Positives = 133/236 (56%), Gaps = 9/236 (3%) Query: 26 ALMTPILLIFFGHSPATAIATDLWFAAITKLVGARMHYAQGNVDWQVAKRLWLGSLPMAL 85 +LMTP+L + FG P+ A+ TDL FA+ TK G H +G V W V +RL G+LP AL Sbjct: 25 SLMTPLLTLLFGIHPSVAVGTDLAFASATKTAGTLAHRFKGTVRWDVVRRLSYGALPAAL 84 Query: 86 LMVFIVGLGGRLIKMEWLT--QAIGVVVLMTATGLLAAPTLAACARRWRIEQPER--FKA 141 + ++ G + LT +I V V +T LL + A W P+R A Sbjct: 85 ITTLLLKHFGAVSDGIALTIRYSIAVSVFLTVIALLFRSRMQA----WLNAHPQRQLQGA 140 Query: 142 VQPALTMAAGAVLGLSVALTSVGAGALGSVMLLYLYPLRMTPHRLVATDIVHAIPLALVA 201 T+AAGA+LG V ++S+GAGA+G+ +L+ LYP R++P + TDI +A+PL +A Sbjct: 141 TLANATIAAGALLGTLVTISSIGAGAVGATLLVLLYP-RLSPAEIAGTDIAYAVPLTAIA 199 Query: 202 GSAYLLAGLVDEWMLLSLLTGSVPAVLLGSLLAGKMAGRALQIALALVLLAGGLKV 257 + G ++ +L +LL GSVP + +GSL A + + L+ LA+ L + +K+ Sbjct: 200 ALGHWWLGSINWELLATLLLGSVPGITIGSLAARAVPEKFLRGLLAITLTSVAVKL 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 662,584 Number of extensions: 25183 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 1 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)