BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0176.p
         (548 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion ...  1100   0.0  
gi|300310625|ref|YP_003774717.1| hemolysin activation translocat...    58   2e-09

>gi|300309648|ref|YP_003773740.1| hemolysin activation/secretion
           protein [Herbaspirillum seropedicae SmR1]
          Length = 569

 Score = 1100 bits (2844), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 541/548 (98%), Positives = 545/548 (99%)

Query: 1   LQHYVMAAALLGTAVAAAAQSVPAAAEATERFEIRRFVVAGNTLLPQDQVQAAVAPFTGQ 60
           LQHYVMAAALLGTAVAAAAQS PAAAEATERFEIRRFVVAGNTLLPQDQVQAAVAPF+GQ
Sbjct: 22  LQHYVMAAALLGTAVAAAAQSAPAAAEATERFEIRRFVVAGNTLLPQDQVQAAVAPFSGQ 81

Query: 61  GRVYGDIQQALEALENLYRSAGYTAVQVHVPEQELSSGEVRIDVVETVVGRVQVSENRHF 120
           GRVYGDIQQALEALENLYRSAGYTAVQVHVPEQELSSGEVRIDVVETVVG+VQVSENRHF
Sbjct: 82  GRVYGDIQQALEALENLYRSAGYTAVQVHVPEQELSSGEVRIDVVETVVGKVQVSENRHF 141

Query: 121 SQANIRASIPALKEGVSPNLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAKIEVKDD 180
           SQANIRASIPALKEGV PNLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAKIEVKDD
Sbjct: 142 SQANIRASIPALKEGVPPNLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAKIEVKDD 201

Query: 181 DPLRVMLNLDNTGSADTGRWRTGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKVDLYSLG 240
           DPLRVMLNLDNTGSADTGRWRTGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKVDLYSLG
Sbjct: 202 DPLRVMLNLDNTGSADTGRWRTGVAIQHSNLFNRDHVATVAYTTSPDSPSGSKVDLYSLG 261

Query: 241 YRLPLYELGDSLDFIYGKSSVSSGQTLALNSTLNITGRGEVYALRWNHFFARNGEWSSKL 300
           YRLPLYELGDSLDFIYGKSSVSSGQTLALNSTLNITGRGEVYALRWNHFFARNGEWSSKL
Sbjct: 262 YRLPLYELGDSLDFIYGKSSVSSGQTLALNSTLNITGRGEVYALRWNHFFARNGEWSSKL 321

Query: 301 VLGADYKKVDSSCAINGGLLNGATLNTCLPYSTLPLSLTYSAQRQGVGQVIDANIGAARN 360
           VLGADYKKVDSSCAINGGLLNGATLNTCLPYSTLPLSLTYSAQRQGVG+VIDANIG ARN
Sbjct: 322 VLGADYKKVDSSCAINGGLLNGATLNTCLPYSTLPLSLTYSAQRQGVGEVIDANIGVARN 381

Query: 361 MATGQARRNDALQVTDRYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSP 420
           MATGQARRNDALQVTDRYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSP
Sbjct: 382 MATGQARRNDALQVTDRYSLFTNGRQSMDNFIAVRGGASWLKAFQNDWQMRLAGNLQLSP 441

Query: 421 HALPAVEQFGLAGATAVRGFTERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDA 480
           HALPAVEQFGLAGATAVRGFTERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDA
Sbjct: 442 HALPAVEQFGLAGATAVRGFTERAVSADSGVVLNSELYTPDLLAKSELKGSMRLLAFVDA 501

Query: 481 GRGANNNVTANSQVQSTLTLASIGAGLRYALGRDFSLRLDVARVLLNGNSITEQRGDLNA 540
           GRGANNNVTANSQVQSTLTLASIGAGLRYALGRDF+LRLDVARVLLNGNSITEQRGDLNA
Sbjct: 502 GRGANNNVTANSQVQSTLTLASIGAGLRYALGRDFNLRLDVARVLLNGNSITEQRGDLNA 561

Query: 541 HLSATLGF 548
           HLSATLGF
Sbjct: 562 HLSATLGF 569


>gi|300310625|ref|YP_003774717.1| hemolysin activation translocator
           protein [Herbaspirillum seropedicae SmR1]
          Length = 581

 Score = 58.2 bits (139), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 63/306 (20%), Positives = 130/306 (42%), Gaps = 17/306 (5%)

Query: 1   LQHYVMAAALLGTAVAAAAQSVPA-AAEATERFEIRRFVVAGNTLLPQDQVQAAVAPFTG 59
           LQ Y +A   L   V     + P   +EA     +RR       + P   +Q  +A + G
Sbjct: 69  LQSYTLAPESLCFPVQRVTLAFPEQVSEA-----VRRTGGFALQMDPFHFIQTYLAGYAG 123

Query: 60  QGRVYGDIQQALEALENLYRSAGYTAVQVHVPEQELSSGEVRIDVVETVVGRVQVSENRH 119
                  I+  L  +       GY+  +V + +Q+LS+G++ I +V  ++  ++  +   
Sbjct: 124 SCLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLVPGIIHAIRFKDPA- 182

Query: 120 FSQANIRASIPALKEGVSPNLRRISESVQLANDNPAKQVNVTLSAAQSGDQIDAKIEVKD 179
              A+ R++ P  + G   NLR + + ++      ++ V++ +       Q D  +EVK 
Sbjct: 183 -ITASYRSAFPT-QAGALLNLRDLEQGLEQMKRVASQDVDMQILPTDVPGQSDVVLEVKR 240

Query: 180 DDPLRVMLNLDNTGSADTGRWRTGVAIQHSNLFNRDHVATVAYTTSPD---SPSGSKVDL 236
             P ++ L+LD++G+  TG+++ G+ +   +L   + +    +++  D      G+  + 
Sbjct: 241 IKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLLGINDLFNAGFSSDADRMGQARGTTGNN 300

Query: 237 YSLGYRLPLYELGDSLDFIYGKSSVSSGQTLALNSTLNITGRGEVYALRWNHFFARNGEW 296
           +S       +  G     + G +S    +   L  T   +G  E   +R    F R+ + 
Sbjct: 301 FSYSAPAGYWTFG-----VSGSTSRYHQRIAGLFQTFVSSGHSENLEVRVQKLFQRDAQQ 355

Query: 297 SSKLVL 302
            + L L
Sbjct: 356 KNSLQL 361


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.130    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,613,401
Number of extensions: 68743
Number of successful extensions: 251
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 248
Number of HSP's successfully gapped: 4
Length of query: 548
Length of database: 1,619,870
Length adjustment: 90
Effective length of query: 458
Effective length of database: 1,193,720
Effective search space: 546723760
Effective search space used: 546723760
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 133 (55.8 bits)