BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0167.p (503 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 991 0.0 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 333 2e-92 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 275 5e-75 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 271 9e-74 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 239 3e-64 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 991 bits (2563), Expect = 0.0, Method: Compositional matrix adjust. Identities = 501/503 (99%), Positives = 502/503 (99%) Query: 1 MTQPLSMSRLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPATQTGENG 60 MTQPLSMSRLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPATQTGENG Sbjct: 2 MTQPLSMSRLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPATQTGENG 61 Query: 61 SQWKTAQPAEDIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARSG 120 SQWKTAQPAEDIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDAR+G Sbjct: 62 SQWKTAQPAEDIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARAG 121 Query: 121 YFPQVDAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATA 180 YFPQVDAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATA Sbjct: 122 YFPQVDAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATA 181 Query: 181 DAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDI 240 DAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDI Sbjct: 182 DAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDI 241 Query: 241 SEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVAVPP 300 SEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAV VPP Sbjct: 242 SEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPP 301 Query: 301 GLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSR 360 GLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSR Sbjct: 302 GLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSR 361 Query: 361 TFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRI 420 TFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRI Sbjct: 362 TFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRI 421 Query: 421 LDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARS 480 LDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARS Sbjct: 422 LDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARS 481 Query: 481 TVNLIRALGGGWGVPAEQLSSAR 503 TVNLIRALGGGWGVPAEQLSSAR Sbjct: 482 TVNLIRALGGGWGVPAEQLSSAR 504 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 333 bits (853), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 209/479 (43%), Positives = 287/479 (59%), Gaps = 19/479 (3%) Query: 19 ALLAALLVLAGCSVAPTYERPSVDT-PAAFKEAAPATQTGENGSQWKTAQPAEDIARGEW 77 A+L A L LA CSV P Y RP +DT PA FKE G WK AQP + +A G+W Sbjct: 14 AMLVASLALAACSVGPDYVRPQMDTGPAIFKE----------GQNWKPAQPRDLVADGKW 63 Query: 78 WKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARSGYFPQVDAGVGATRQRPS 137 W++FGD +LNAL +NQ+L A A A AL + +RS Y P + A GA+R Sbjct: 64 WEMFGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSG 123 Query: 138 PASQGLPADADTRPNTLYRAQVNVS----YEVDLFGRVASTVDAATADAQRSEALFHSVL 193 A A R S +E D++G+V V+++ A AQ S A + Sbjct: 124 SAGASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAAR 183 Query: 194 LALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELA 253 L+ QA VAQ Y +R LDA L TV + SL++ Q ++ G +++ DV +A+ +L Sbjct: 184 LSAQAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLK 243 Query: 254 SAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVAVPPGLPSALLERRPDI 313 SAQ+ AL RA EHA+A+L+G+TPA+F+LP PL +A VP G+PS LLERRPD+ Sbjct: 244 SAQASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERRPDV 303 Query: 314 AAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALS 373 AAAER +A+ANA++GVAK+A+FPSL L+ GY+S+ D F R + LGP L+ Sbjct: 304 AAAERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGP----QLA 359 Query: 374 MPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVA 433 +FDGG R A D+A A+Y+ VA+Y+ TVL AF+EVEDNLA LRIL+Q+ Q EA+ Sbjct: 360 QTIFDGGARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQ 419 Query: 434 ASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 492 A+ +A L + QY+ G VSY +VI A T L + +S+ + R ++V L +ALGGGW Sbjct: 420 AARQAVTLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGW 478 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 275 bits (704), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 194/469 (41%), Positives = 263/469 (56%), Gaps = 22/469 (4%) Query: 29 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 88 GC+ P Y RP VD PAA+ AP W++ QPA+ RG WW+ FGD +LNA Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAP----------WRSMQPADATPRGPWWERFGDAQLNA 67 Query: 89 LEDTAQQANQDLKAAAARLGQARALTRDARSGYFPQVDAGVGATRQRPSPASQGLPADAD 148 L+ A NQ L A ARL QARA + PQV+ A R R S P Sbjct: 68 LQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSR---ISANRPLTNY 124 Query: 149 TRPN-----TLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQ 203 PN + +VSYEVD FGRV +V+AA A A++S A + L L A++A Sbjct: 125 NSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATN 184 Query: 204 YFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIA 263 YF +RELD + R + L++++L L R D G S +D+A+ +A L S ++ + Sbjct: 185 YFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLG 244 Query: 264 RQRATAEHALAILLGRTPAEFSLPPQPLTRLAVAVPPGLPSALLERRPDIAAAERAMAAA 323 RQRA EHA+A L G FS+ P A+P G+PS +LERRPDIAAAERAMAAA Sbjct: 245 RQRAQYEHAIATLTGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERAMAAA 304 Query: 324 NARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQ 383 NA++GVA +A++PS L G +S LFN S + L G + S LFDGGR + Sbjct: 305 NAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSL----GVSASQSLFDGGRLR 360 Query: 384 AGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSH 443 AGVD ++A YE VASYR TVL A +EVED + L L++ A+A++ R +++ Sbjct: 361 AGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIAS 420 Query: 444 TQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 492 ++Y GA Y VI A +++L +R + L G+R +V LI+ALGG W Sbjct: 421 SRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 271 bits (693), Expect = 9e-74, Method: Compositional matrix adjust. Identities = 198/497 (39%), Positives = 277/497 (55%), Gaps = 38/497 (7%) Query: 13 PVRIGSALLAALL--VLAGCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAE 70 P R + A +L +L GC+V P Y RP++D P A+KE P WK A P + Sbjct: 28 PFRATKGMTALVLAALLGGCAVGPDYARPALDLPQAYKETGP----------WKLAAPRQ 77 Query: 71 DIARGEWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARSGYFPQVDAGVG 130 WW+ +GD LNAL D A ANQ ++ AAA+ QA+A AR+ +P + A G Sbjct: 78 IDDHQPWWEAYGDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAG 137 Query: 131 ATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFH 190 A+R + + + R Y +N S+E DL+GR+ +A A+AQ S A Sbjct: 138 ASRAQTN-------TNGVQRLGNSYSVGLNASWEADLWGRIRRGAEAGEANAQASAASLA 190 Query: 191 SVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARA 250 + L++QA +AQ Y +R D LYR TV SL+L +++ G DVA+A Sbjct: 191 AARLSIQATLAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAET 250 Query: 251 ELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQP---------------LTRLA 295 +L +AQ++ + + R EHA+A+L+G+ PA+FSLP P L Sbjct: 251 QLRAAQAQLIDLDDTRNQLEHAIAMLIGKAPAQFSLPALPPAAASDSAIDANAARLQGSL 310 Query: 296 VAVPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLF 355 +P GLPS LLERRPDIA AER AAANA +GVA++A+FP+L L+ GY S +LF Sbjct: 311 PQIPVGLPSELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLF 370 Query: 356 NWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNL 415 N SR + LG +AL+ LFDGG R A D A A+Y+ VA Y+ TVL +EVED L Sbjct: 371 NTPSRVWSLG----SALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKL 426 Query: 416 ANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDG 475 + LR+LDQ++ Q +AV ++ A +L+ QY G V+Y SV+ L QR +VTL G Sbjct: 427 STLRVLDQESTVQAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLG 486 Query: 476 ERARSTVNLIRALGGGW 492 + ++V LI+A GGGW Sbjct: 487 RQLVASVALIKATGGGW 503 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 239 bits (610), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 175/500 (35%), Positives = 255/500 (51%), Gaps = 45/500 (9%) Query: 5 LSMSRLLRPVRIGSALLAALLVLAGCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWK 64 LSM R R + + +AL+ A VL GCS+APTYERP +++ Sbjct: 4 LSMPRSPRLLHMCAALVMAG-VLGGCSLAPTYERPQAPVESSY----------------- 45 Query: 65 TAQPAEDIARGE-------WWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDA 117 PA+ R E W + F D++L L A + N+DL+ A + +ARA + A Sbjct: 46 ---PADAAGRAELQAVNLGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIA 102 Query: 118 RSGYFPQVDAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVS---YEVDLFGRVAST 174 R+ P ++ A R R + ASQ L +P QV ++ YE+D FGRV + Sbjct: 103 RADLLPNLNLSGNAARTR-TAASQSLTG----QPTISNSYQVGLAVPAYELDFFGRVRNL 157 Query: 175 VDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRR 234 DAA A +E S ++L A VAQ Y R L T+ RQ +L L Q+R Sbjct: 158 KDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKR 217 Query: 235 FDEGDISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTPAEFSLPPQPL--- 291 D G + +D + + SA+ + RQRA AE+AL +L+G+ E LPP L Sbjct: 218 LDVGASTALDFRLTQTLVESARVAKTQLERQRAQAENALTLLVGKKIGE--LPPAQLLSD 275 Query: 292 TRLAVAVPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQL 351 ++ +P GLPS LL RPDI +AE+++ AANA +G A++AFFP ++LTG G S+ L Sbjct: 276 EKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTL 335 Query: 352 GDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREV 411 G LF S + GP +LS+P+FD GR A +D A + V +Y + AFREV Sbjct: 336 GGLFESGSGAWSFGP----SLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREV 391 Query: 412 EDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASV 471 D L LD+Q +AQ+ +AA KL+ +++ G S +DA R + Q+A + Sbjct: 392 ADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALI 451 Query: 472 TLDGERARSTVNLIRALGGG 491 R + ++L +ALGGG Sbjct: 452 QARQLRLNNAIDLYKALGGG 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,355,273 Number of extensions: 51168 Number of successful extensions: 325 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 260 Number of HSP's successfully gapped: 18 Length of query: 503 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 414 Effective length of database: 1,198,455 Effective search space: 496160370 Effective search space used: 496160370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)