BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0165.p (399 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 773 0.0 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 174 2e-44 gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 146 3e-36 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 145 8e-36 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 139 4e-34 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 773 bits (1996), Expect = 0.0, Method: Compositional matrix adjust. Identities = 392/399 (98%), Positives = 393/399 (98%) Query: 1 MQTPAPLRKRFALTATVLAVIALSVGGAISVVGLSANATTAPSAAAAGAPVDVAEVVSRQ 60 MQTPAPLRKRFALTATVLAVIALSVGGAISVVGLSANATTAPSAAAAGAPVDVAEVVSRQ Sbjct: 1 MQTPAPLRKRFALTATVLAVIALSVGGAISVVGLSANATTAPSAAAAGAPVDVAEVVSRQ 60 Query: 61 IIDWQSYSGRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAE 120 IIDWQSYSGRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAE Sbjct: 61 IIDWQSYSGRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAE 120 Query: 121 AQLAGAEARAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARL 180 AQLAGAEARAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARL Sbjct: 121 AQLAGAEARAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARL 180 Query: 181 NLEYTHITAPVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVN 240 NLEYT ITAPVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVN Sbjct: 181 NLEYTRITAPVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVN 240 Query: 241 PARAAGATVPVELGLANEDGYSRKGRVGSVDNRLDTASGTIRVRAIFDNADSQLVPGLYA 300 PARAAGATVPVELGLANEDGYSRKGRVGSVDNRLDTASGTIRVRAIFDNA+SQLVPGLYA Sbjct: 241 PARAAGATVPVELGLANEDGYSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYA 300 Query: 301 RVRLGGGEPHQAVLIDEKAIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQ 360 RVRLGGGEPHQAVLIDEKAIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQ Sbjct: 301 RVRLGGGEPHQAVLIDEKAIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQ 360 Query: 361 PGERIVVNGLQRIRPGDAITPTVVPMQTQTAAAPAAGKA 399 PGERIVVNGLQRIRPGDAITPTVVPMQT A A AGKA Sbjct: 361 PGERIVVNGLQRIRPGDAITPTVVPMQTAAAPAAGAGKA 399 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 174 bits (440), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 125/363 (34%), Positives = 186/363 (51%), Gaps = 18/363 (4%) Query: 45 AAAGAP-----VDVAEVVSRQIIDWQSYSGRIEAVDRVEVRPLVGGTLTAVHFKDGALVK 99 AA G P V V + +++ GR+EA +VR V G + FK+G+ VK Sbjct: 28 AAQGGPMPPSEVSVVTIAPQRVAITNELPGRVEAFRVAQVRARVAGIVQKRVFKEGSEVK 87 Query: 100 KGDVLFTIDPRPYEAEVARAEAQLAGAEARAGYTASDLARGQRLLSDNAIAKRDFEEKQN 159 +G VLF IDP Y+A + A+A LA A+A T R + L+ NA++K++++ Sbjct: 88 EGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVA 147 Query: 160 ASREASANVAAARAALRAARLNLEYTHITAPVSGRISRAEVTVGNVVNPGAANAPLTTLV 219 A ++A A+VAA +AA++ A LNL Y +T+P+SGRI RA VT G +V G A L + Sbjct: 148 AQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQ-LALVQ 206 Query: 220 SVAKVYASFDVDEQSFLKYVNPARAAGA-------TVPVELGLANEDGYSRKGRVGSVDN 272 + VY + LK + A AAG V L L N Y G++ D Sbjct: 207 QLDPVYVNVTQSSADLLK-LQQAMAAGQLKSVGDNKASVTLELENGQAYPLPGKLLFSDI 265 Query: 273 RLDTASGTIRVRAIFDNADSQLVPGLYARVRLGGGEPHQAVLIDEKAIGTDQDKRFVMVA 332 +D +G++ +RA F N L+PG Y R ++ QA+L+ ++A+ D VMV Sbjct: 266 SVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDAGGSTVMVV 325 Query: 333 DKDNHANYRQVVVGAGHDGLVVIEKGLQPGERIVVNGLQRIRPGDAITPTVVPMQTQTAA 392 + +N R V G + GL+ G+R++V GLQ++RPG TV P+Q A Sbjct: 326 NAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGA----TVKPVQWNPAG 381 Query: 393 APA 395 APA Sbjct: 382 APA 384 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 146 bits (368), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 103/318 (32%), Positives = 159/318 (50%), Gaps = 10/318 (3%) Query: 69 GRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEA 128 GR A ++RP VGG + FK+G+ VK G L+ IDP Y+A A A L AEA Sbjct: 67 GRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEA 126 Query: 129 RAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTHIT 188 + R L +A++K+D ++ Q+ R+ A+V +AA++ A +NL+YT I Sbjct: 127 TLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKIL 186 Query: 189 APVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKY-----VNPAR 243 +P+SGRI ++ VT G +V A LTT+ + +Y L+ R Sbjct: 187 SPISGRIEKSAVTPGALVTASQTTA-LTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIR 245 Query: 244 AAGATVPVELGLANEDG--YSRKGRVGSVDNRLDTASGTIRVRAIFDNADSQLVPGLYAR 301 +G ++ + EDG Y R G++ +DT +G I +RA+ N D L+PG Y R Sbjct: 246 KSGENA-AQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVR 304 Query: 302 VRLGGGEPHQAVLIDEKAIGTDQD-KRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQ 360 L G A+L +KA+ DQ +V D R V V + +GL+ Sbjct: 305 AVLEEGVDDHALLAPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLK 364 Query: 361 PGERIVVNGLQRIRPGDA 378 G++++V+GLQ++R GDA Sbjct: 365 EGDKLIVDGLQKVRTGDA 382 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 145 bits (365), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 94/298 (31%), Positives = 147/298 (49%), Gaps = 2/298 (0%) Query: 79 VRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEARAGYTASDLA 138 VR V G L +H+K+G +VK GD+L IDPR +A + +AE QLA A DL Sbjct: 62 VRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLK 121 Query: 139 RGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTHITAPVSGRISRA 198 R Q LL+ ++IA + + + ++ V A + +ARL L +T +TAP+SGR+ Sbjct: 122 RYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGLR 181 Query: 199 EVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVNPARAAGATVPVELGLANE 258 + VGN V N L + + + A F + E + K + + +G +P + + Sbjct: 182 QADVGNNVTTSDTNG-LVIITQLQPITAIFSIPEDNIPKVLQQLQ-SGRKLPAQAWDREQ 239 Query: 259 DGYSRKGRVGSVDNRLDTASGTIRVRAIFDNADSQLVPGLYARVRLGGGEPHQAVLIDEK 318 G + ++DN +D +GT++++A F N D L P + +RL A +I Sbjct: 240 KNKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTA 299 Query: 319 AIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQPGERIVVNGLQRIRPG 376 AI FV V D+ R V G D L VI G+ GE +V++G+ R+R G Sbjct: 300 AIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREG 357 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 139 bits (350), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 103/327 (31%), Positives = 168/327 (51%), Gaps = 15/327 (4%) Query: 69 GRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEA 128 GR +VRP V G + A F++G+ VK G VL+ I+P Y+A + A LA AEA Sbjct: 59 GRTSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEA 118 Query: 129 RAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTHIT 188 +R + L++ A++++D+++ + EA A+V +ARA ++ +R+NL+Y + Sbjct: 119 SVRTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLD 178 Query: 189 APVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKYVNPARA---- 244 AP+SGRI ++ VT G +V A LTT+ + +Y DV QS ++N A Sbjct: 179 APISGRIGKSSVTPGALVTANQTTA-LTTIQQLDPIY--VDV-TQSSTTWLNLKEALANG 234 Query: 245 ----AGATVPVELGLANEDGYSRKGRVGSVDNRLDTASGTIRVRAIFDNADSQLVPGLYA 300 +G V L L N Y+++G + D ++ +G I +RAIF N + L+PG+Y Sbjct: 235 QLEKSGNGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYV 294 Query: 301 RVRLGGGEPHQAVLIDEKAIGTD-QDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGL 359 R L G Q +L+ ++A+ D K V + R +V ++ GL Sbjct: 295 RAVLQEGVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGL 354 Query: 360 QPGERIVVNGLQRIRPGDAITPTVVPM 386 + G+++VV+G QR G + VVP Sbjct: 355 KEGDQLVVDGQQRASAGAKV--KVVPW 379 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,020,282 Number of extensions: 38359 Number of successful extensions: 186 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 177 Number of HSP's successfully gapped: 11 Length of query: 399 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 312 Effective length of database: 1,207,925 Effective search space: 376872600 Effective search space used: 376872600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)