BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0164.p
         (287 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro...   575   e-165
gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi...   115   6e-27
gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum...    96   3e-21

>gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily
           protein [Herbaspirillum seropedicae SmR1]
          Length = 332

 Score =  575 bits (1481), Expect = e-165,   Method: Compositional matrix adjust.
 Identities = 285/287 (99%), Positives = 286/287 (99%)

Query: 1   MQGSSSLDIRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTP 60
           MQGSSSLDIRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTP
Sbjct: 46  MQGSSSLDIRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTP 105

Query: 61  ARFIAEHGQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDA 120
           ARFIAEHGQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDA
Sbjct: 106 ARFIAEHGQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDA 165

Query: 121 GGCLAASLTLMTRDRCGKPLAAQVLISPMLDPSMRLLGDAARLQSDLTAETCAASYRQYL 180
           GGCLAASLTLMTRDRCGKPLAAQVLISPMLDPSMRLLGDAARLQSDLTAETCAASYRQYL
Sbjct: 166 GGCLAASLTLMTRDRCGKPLAAQVLISPMLDPSMRLLGDAARLQSDLTAETCAASYRQYL 225

Query: 181 PQTMQRLHPYAAPLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAG 240
           PQTMQRLHPYAAPLEVSRLAGLPPAFIATAE DVLCPEAEKYASSLIRAGVPTQVSRFAG
Sbjct: 226 PQTMQRLHPYAAPLEVSRLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFAG 285

Query: 241 ITHAALRTHLPLLTEIVNFLRCRFAKLQGHAPHPQFLPNKLQPSTPF 287
           ITHAALRTHLPLLTEIVNFLRCRFAKLQGHAPHP+FLPNKLQPSTPF
Sbjct: 286 ITHAALRTHLPLLTEIVNFLRCRFAKLQGHAPHPEFLPNKLQPSTPF 332


>gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae
           SmR1]
          Length = 340

 Score =  115 bits (287), Expect = 6e-27,   Method: Compositional matrix adjust.
 Identities = 71/209 (33%), Positives = 102/209 (48%), Gaps = 5/209 (2%)

Query: 39  PLVMYFHGGGFVSGSLEDADTPAR-FIAEHGQLAVLSVGYALAPAKPFPAAPEDAFAAAT 97
           P  M+FHGGG+V G     +   R  +A  G +AV  V Y  +P   +  A   A+AA  
Sbjct: 103 PAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF-VNYTPSPEAHYGVAINQAYAATK 161

Query: 98  WACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGKPLAAQVLISPMLDPSMRLL 157
           W  ++  E+ VD  R+ VAG+  GG +AA + LM +D+ G  L ++VL+ P+ D +    
Sbjct: 162 WVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETA 221

Query: 158 GDAARLQSD-LTAETCAASYRQYLPQTMQRLHPYAAPLEVS--RLAGLPPAFIATAECDV 214
                     LT       +  Y     +R    A+PL  +  +L+GLPP  I TAE DV
Sbjct: 222 SYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDV 281

Query: 215 LCPEAEKYASSLIRAGVPTQVSRFAGITH 243
           L  E E+Y   L  AGV     R+ G+ H
Sbjct: 282 LRDEGEEYGRKLAAAGVAVTSVRYNGMIH 310


>gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum
           seropedicae SmR1]
          Length = 317

 Score = 95.9 bits (237), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 85/231 (36%), Positives = 113/231 (48%), Gaps = 7/231 (3%)

Query: 20  RIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHGQLAVLSVGYAL 79
           RI  RLY PA   A    +P+++YFHGGG+  GSL+  DT    +A      VL+V Y L
Sbjct: 67  RIGARLYAPAPLDAA-APQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLAVDYRL 125

Query: 80  APAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGKP 139
           AP   FP A EDA  A  W  ++   L +  +R+ V GD AGG LAA+L L  R     P
Sbjct: 126 APEHRFPTAFEDAADAYDWLLQHGPALGLATNRLAVGGDSAGGTLAAALALRARQAARAP 185

Query: 140 LAAQVLISPMLDPSMRLLGDAARLQSD---LTAETCAASYRQYLPQTMQRLHPYAAPLEV 196
              QVL+ P    S    GD+ R  +    L  ET    +   L    +R     APL+ 
Sbjct: 186 -CLQVLLYPC--TSAHQDGDSHRRFATGYLLEGETLQWMFGHTLNGDHERQDWRFAPLQA 242

Query: 197 SRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGITHAALR 247
           + ++G  PA +   + D L  E   YA  L +AGVP Q+    G+ H  LR
Sbjct: 243 ADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLR 293


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.137    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 876,061
Number of extensions: 34781
Number of successful extensions: 94
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 94
Number of HSP's successfully gapped: 4
Length of query: 287
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 203
Effective length of database: 1,222,130
Effective search space: 248092390
Effective search space used: 248092390
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 130 (54.7 bits)