BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0163.p (337 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309660|ref|YP_003773752.1| LysR family transcription regul... 702 0.0 gi|300313746|ref|YP_003777838.1| LysR family transcription regul... 269 2e-73 gi|300311868|ref|YP_003775960.1| LysR family transcription regul... 246 1e-66 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 203 2e-53 gi|300312196|ref|YP_003776288.1| LysR family transcription regul... 180 2e-46 >gi|300309660|ref|YP_003773752.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 337 Score = 702 bits (1811), Expect = 0.0, Method: Compositional matrix adjust. Identities = 337/337 (100%), Positives = 337/337 (100%) Query: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP Sbjct: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 Query: 61 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD 120 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD Sbjct: 61 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD 120 Query: 121 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT 180 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT Sbjct: 121 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT 180 Query: 181 IEDLQQHKAVHYFSSRTGRTIDWDFVVEGVTREVKMNGKLAVNEADAYVECALQGFGMVQ 240 IEDLQQHKAVHYFSSRTGRTIDWDFVVEGVTREVKMNGKLAVNEADAYVECALQGFGMVQ Sbjct: 181 IEDLQQHKAVHYFSSRTGRTIDWDFVVEGVTREVKMNGKLAVNEADAYVECALQGFGMVQ 240 Query: 241 APLYMVTRHIDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVRAFVDWVAELFATCPLH 300 APLYMVTRHIDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVRAFVDWVAELFATCPLH Sbjct: 241 APLYMVTRHIDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVRAFVDWVAELFATCPLH 300 Query: 301 KACTMGMEESECRFVDKAETSTVRNVVEQYNIAESVF 337 KACTMGMEESECRFVDKAETSTVRNVVEQYNIAESVF Sbjct: 301 KACTMGMEESECRFVDKAETSTVRNVVEQYNIAESVF 337 >gi|300313746|ref|YP_003777838.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 269 bits (688), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 139/292 (47%), Positives = 204/292 (69%), Gaps = 3/292 (1%) Query: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 MD+ A++ F VV+A +FTRAAD+L++P T++ LV+ LE+ L VRLL RTTRR+S+TP Sbjct: 1 MDQLLAIRAFARVVEAGNFTRAADSLDMPNATLSKLVRELEAHLGVRLLQRTTRRVSVTP 60 Query: 61 DGAAYYERCVRILADLDETEQSFRNVTRG-PQGRLRIDVPASVGRLMLLPNLCDFYTRYP 119 +G YYE+ R++ DL++ + SF N RG P+G+LRIDV S R +L+P L DF RYP Sbjct: 61 EGLDYYEKTGRLVRDLEDIDTSF-NAARGNPRGKLRIDVGGSTARDVLIPALPDFLARYP 119 Query: 120 DIELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPH 179 DI + +G+ DRPVDL+ ++VDCVIR G L +SS+VAR+IG + VTCA PAYL++ GVP Sbjct: 120 DISIDLGVSDRPVDLIGDSVDCVIRGGPLDNSSLVARQIGQARMVTCATPAYLKQFGVPA 179 Query: 180 TIEDLQQ-HKAVHYFSSRTGRTIDWDFVVEGVTREVKMNGKLAVNEADAYVECALQGFGM 238 ++L+ H+ + Y S ++GR + F +G E+K ++ VNE++A++ AL G G+ Sbjct: 180 YPDELKNGHRLISYLSPQSGRAFPFRFERKGEKIEIKTEHRIGVNESNAHLAAALAGLGI 239 Query: 239 VQAPLYMVTRHIDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVRAFVDWV 290 VQ Y + G LVE+L++W+PAP P VVY +NRH+S ++R F+DW+ Sbjct: 240 VQTFRYAAGPALARGELVEILTKWRPAPYPFHVVYPNNRHVSHRLRVFIDWL 291 >gi|300311868|ref|YP_003775960.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 307 Score = 246 bits (629), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 119/296 (40%), Positives = 175/296 (59%), Gaps = 2/296 (0%) Query: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 M + +++F V + SF+ AA L LP +V+ +Q LE ML RLL RTTRR+ LT Sbjct: 1 MHQLDTLRIFQRVAELASFSGAARQLGLPNASVSLAIQQLEQMLGTRLLQRTTRRVQLTA 60 Query: 61 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD 120 DG A+Y R +L + + F + + GRLR+D+ +++ R ++LP L F ++P Sbjct: 61 DGEAFYRRSRELLDEFESLRGMFHDGAQALSGRLRVDMSSAMAREVVLPALPSFLDKHPQ 120 Query: 121 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT 180 +E+ + DR VDLV E DCV+R G L DSS+VAR IG + CA+P YL R+G P T Sbjct: 121 LEIELSASDRRVDLVAEGFDCVLRTGTLDDSSLVARPIGHLVQINCASPEYLRRYGTPQT 180 Query: 181 IEDLQQHKAVHYFSSRTGRTIDWDFVVEGVTREVKMNGKLAVNEADAYVECALQGFGMVQ 240 +EDL QH+ VHY GR+ W++ TR + M G + VN + Y L G G++Q Sbjct: 181 LEDLAQHRLVHYRQVLGGRSPGWEWFDGQQTRSLPMAGSITVNSTETYYAACLAGLGLIQ 240 Query: 241 APLYMVTRH--IDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVRAFVDWVAELF 294 PL + +D G +VEVL Q++PAPMP+S++Y RH+ ++ F+DW+ L Sbjct: 241 LPLVGAQKRQMLDGGHVVEVLPQYRPAPMPVSLLYASRRHVPARLAGFMDWLHALI 296 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 203 bits (516), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 107/295 (36%), Positives = 169/295 (57%), Gaps = 2/295 (0%) Query: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 M++ + ++ F AV D SF +AA+ L + + V+ V LE+ L RL+ RTTRR+SLT Sbjct: 1 MNKLREIECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTE 60 Query: 61 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD 120 GA Y ERC +I+A L+E + + + P G LRI+ P + G L L P F R+P+ Sbjct: 61 AGALYLERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPE 120 Query: 121 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT 180 I L + + DR VD++ E D IRI L DS++V R++ + CA+PAYL+ HG P Sbjct: 121 ISLDITLSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAH 180 Query: 181 IEDLQQHKAVHYFSSRTGRTIDWDFVVEGVTREVKMNGKLAVNEADAYVECALQGFGMVQ 240 +L QH+ + Y +++ +W F V+++ +L VN D+ V+ AL G G+ + Sbjct: 181 PHELAQHQTISYSYNQSKD--EWQFNGPEGPVSVRVSSRLHVNNGDSCVQAALGGIGITR 238 Query: 241 APLYMVTRHIDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVRAFVDWVAELFA 295 P +M+ +++ G+LV +L + + I VY HL K+RA +D++ E FA Sbjct: 239 QPTFMIDQYLRSGQLVSILGDYGAPELGIYAVYPSRAHLPAKMRAMLDFLTEAFA 293 >gi|300312196|ref|YP_003776288.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 180 bits (456), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 110/300 (36%), Positives = 155/300 (51%), Gaps = 4/300 (1%) Query: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 MD+ QAMQ F AVV++ SF A D + V+ V LE L +RLL+RTTRR+SLT Sbjct: 1 MDKLQAMQTFVAVVESGSFVAAVDVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 Query: 61 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD 120 +G Y+ RC +L + + E + GRLRI P G L P F ++P Sbjct: 61 EGRVYFARCKELLETIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQHPL 120 Query: 121 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT 180 I L + + DR +DL++E D IRI L DS MVAR + + + CAAP +L RHG P T Sbjct: 121 ITLNITLSDRTIDLLEEGFDMAIRISALGDSRMVARPLAPVRMIACAAPEFLARHGTPGT 180 Query: 181 IEDLQQHKAVHYFSSRTGRTIDWDFV-VEGVTREVKMNGKLAVNEADAYVECALQGFGMV 239 +L+ + + Y S R G DW F V G T +V++ + VN A+ G G+ Sbjct: 181 PAELRDYPFISY-SYRAGGD-DWSFRHVHGDTCQVRVRSTVYVNNGATCRAMAIAGQGIA 238 Query: 240 QAPLYMVTRHIDEGRLVEVLSQWKPAP-MPISVVYLHNRHLSPKVRAFVDWVAELFATCP 298 P ++V I G+LVE+ W + + +Y HL PKV+ D++ E F P Sbjct: 239 IQPDFIVNAAIQAGQLVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDFLIEQFRVPP 298 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 978,741 Number of extensions: 39465 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 52 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 52 Length of query: 337 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 251 Effective length of database: 1,212,660 Effective search space: 304377660 Effective search space used: 304377660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 131 (55.1 bits)