BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0160.p (335 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 694 0.0 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 154 1e-38 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 144 1e-35 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 139 3e-34 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 101 7e-23 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 694 bits (1792), Expect = 0.0, Method: Compositional matrix adjust. Identities = 333/334 (99%), Positives = 333/334 (99%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 Query: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHR 120 IVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHR Sbjct: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILYMHR 120 Query: 121 DYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL 180 DYQDENLEEAVQALGDLIR GKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL Sbjct: 121 DYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNL 180 Query: 181 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFR 240 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFR Sbjct: 181 LNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFR 240 Query: 241 EESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKIS 300 EESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKIS Sbjct: 241 EESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKIS 300 Query: 301 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRVVR 334 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRVVR Sbjct: 301 AEEEALVDSLVTPGHASTHGYNDPNYPFFGRVVR 334 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 154 bits (388), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 114/324 (35%), Positives = 169/324 (52%), Gaps = 15/324 (4%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGR-IVASAREHGVNFIDTADVY--TKGK 57 M YRR G+S L + A+ LG D E+GR ++ A + GV D A+ Y G Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGS 73 Query: 58 SEEIVGKLLAGQ----RHEWVLASKLGNAMSGKP-NESNYSRSWIVRETENILTRLDTDY 112 +EE G+ +A R E V++SK G M P + +R ++ E L R+ DY Sbjct: 74 AEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIASCEQSLKRMGLDY 133 Query: 113 LDILYMHRDYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPL 172 +DI Y HR D LEE + AL L R GK G+S++ + + + VPL L Sbjct: 134 VDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPL-L 192 Query: 173 VCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPG-QKPAQDTRAGRGD 231 + QP Y++LNR E +LPA G+G + +SP+A+G+LT KY G + A+ TRA Sbjct: 193 IHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGARVTRAPSLP 252 Query: 232 KRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDY 291 +RM+ E++L + L + RG L Q A AWVL ++ ++S + G RT+ Q++D Sbjct: 253 QRMLS----EDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDS 308 Query: 292 YDAA-ELKISAEEEALVDSLVTPG 314 DA +L + EE A +D T G Sbjct: 309 LDAVNKLDFTQEELASIDKHATDG 332 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 144 bits (363), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 105/332 (31%), Positives = 163/332 (49%), Gaps = 26/332 (7%) Query: 4 RRLGKSNLKVSALCLGSMMFGD---QTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 RRLG + VSA+ LG M D Q D ES + A E G+N +DTAD+Y +EE Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEE 77 Query: 61 IVGKLLAGQRHEWVLASKLGNAMSGKPNES-----NYSRSWIVRETENILTRLDTDYLDI 115 ++G+ + G+R ++ +A+K G + P++ + S ++I + E L RL + +D+ Sbjct: 78 LLGRAIKGRRDKFFIATKFG--IRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDL 135 Query: 116 LYMHRDYQDENLEEAVQALGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQ 175 Y HR +E + L DL++AGKIR G+S A L + V Q Sbjct: 136 YYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSE------ASAATLERAHRVHPVTALQ 189 Query: 176 PYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMM 235 Y+L R PE E+L AC G+G V YSP+ RG LTG Q + A+D D R Sbjct: 190 SEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAED------DFRRS 243 Query: 236 ETEFREE----SLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDY 291 F+ E +L +A+++++ G Q A AWV+A I G + +ED Sbjct: 244 NPRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEDN 303 Query: 292 YDAAELKISAEEEALVDSLVTPGHASTHGYND 323 + +K++ E+ +D++ G A+ Y D Sbjct: 304 AGSVGVKLAPEDLQALDAIFPRGAAAGERYTD 335 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 139 bits (350), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 101/348 (29%), Positives = 174/348 (50%), Gaps = 46/348 (13%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVY------- 53 M YR LG+++LKVS + LG+M +G+Q ++ + A E G+N ID A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 54 TKGKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNE--------SNYSRSWIVRETEN 103 T+G +E +G L +G+R + ++A+K + KP+ +N R + Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKC-TGPARKPHNPRHVRGGINNLDRKGLTEALHG 119 Query: 104 ILTRLDTDYLDILYMH--------------RDYQDEN---LEEAVQALGDLIRAGKIRGF 146 L RL D++D+ +H + +DE+ +EE + L + ++AGKIR Sbjct: 120 SLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTI 179 Query: 147 GVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPI 206 G+SN W +A+ +R E++ + + Q YNLLNR E+ + +G++ YSP+ Sbjct: 180 GLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPL 239 Query: 207 ARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFREESLLIAQRLQQHCEA----RGVKLG 262 A G+L+GKY G +PA G + + F + A+R A G+ Sbjct: 240 AFGMLSGKYLDGARPA-------GGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPA 292 Query: 263 QFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKISAEEEALVDSL 310 Q A A+V + ++S I G TLAQ+ D+AEL++ + A ++++ Sbjct: 293 QMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAI 340 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 101 bits (252), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 93/316 (29%), Positives = 147/316 (46%), Gaps = 22/316 (6%) Query: 1 MHYRRLGKSNLKVSALCLGSMMFGDQ---TDLQESGRIVASAREHGVNFIDTADVYTKGK 57 M RR G++ +VS + G+ G Q++ + +A + GV+FIDTADVY G+ Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 58 SEEIVGKLL---AGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLD 114 SE+++ ++L G+R +A+K G +S R+ + + L L D LD Sbjct: 61 SEQLIAQVLKERGGERP--FVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLD 118 Query: 115 ILYMHRDYQDENLEEAVQA-LGDLIRAGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLV 173 ++ +H + ++ V L L+ AGKIR +GVS ++ E ++ E NV Sbjct: 119 LVQLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSV---EKVEEALKAIEYPNVK---S 172 Query: 174 CQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQD-----TRAG 228 Q +N+ ++P L + V+ P+A G+LTGK A D R G Sbjct: 173 VQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHG 232 Query: 229 RG-DKRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQ 287 D + E L A + +GV + QFA W+L S IS+VI G R +AQ Sbjct: 233 EAFDVGETFSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQ 292 Query: 288 MEDYYDAAEL-KISAE 302 + A+ L I+AE Sbjct: 293 AQSNSAASALPPITAE 308 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,080,324 Number of extensions: 45019 Number of successful extensions: 130 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 6 Length of query: 335 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 249 Effective length of database: 1,212,660 Effective search space: 301952340 Effective search space used: 301952340 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)