BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0154.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase [Her... 778 0.0 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 68 1e-12 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 65 6e-12 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 65 7e-12 gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 62 9e-11 >gi|300309667|ref|YP_003773759.1| enoyl-coenzyme A hydratase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 778 bits (2009), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/386 (98%), Positives = 383/386 (99%) Query: 1 MNQAAAPVVFEELTAANGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVI 60 MNQAAAPVVFEELTAANGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMV+ Sbjct: 1 MNQAAAPVVFEELTAANGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVV 60 Query: 61 LQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKP 120 LQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKP Sbjct: 61 LQAQGEKAFCAGGDLQQLYRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKP 120 Query: 121 ILCWGHGIVMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIF 180 ILCWGHGIVMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIF Sbjct: 121 ILCWGHGIVMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIF 180 Query: 181 LALTGANLNAQDALFAGLADYGMAHASKAAVFDALLSQPWGQVRDAELLDRVLAGAQAAS 240 LALTGANLNAQDALFAGLADYGMAHASKAAVFDALLSQPWGQ RDAELLDRVLAGAQAAS Sbjct: 181 LALTGANLNAQDALFAGLADYGMAHASKAAVFDALLSQPWGQGRDAELLDRVLAGAQAAS 240 Query: 241 RREGVAAFVPSNLRSHFDQIEALCRRPTLPEIVDAILAVQTDDAWLLKAQSTLRAGAPGS 300 RREGVAAFVPSNLRSHFDQIEALCRRPTLPEIVDAILAVQTDDAWLLKAQSTLRAGAPGS Sbjct: 241 RREGVAAFVPSNLRSHFDQIEALCRRPTLPEIVDAILAVQTDDAWLLKAQSTLRAGAPGS 300 Query: 301 AWLGYALQKKVRPLSLADVFRLEFVVSLHCAARPDFVEGIRALIIEKDHKPQWKPASLKQ 360 AWLGYALQKKVRPLSLA+VFRLEFVVSLHCAARPDFVEGIRALIIEKD KPQWKPASLKQ Sbjct: 301 AWLGYALQKKVRPLSLAEVFRLEFVVSLHCAARPDFVEGIRALIIEKDQKPQWKPASLKQ 360 Query: 361 ATPAWVEGFFADPWAQGGHPLADLGR 386 ATPAWVEGFFADPW QGGHPLADLGR Sbjct: 361 ATPAWVEGFFADPWTQGGHPLADLGR 386 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 56/187 (29%), Positives = 85/187 (45%), Gaps = 22/187 (11%) Query: 22 GIATLASEKTLH--AISLEMVQLLTPQLQHWQADPEVAMVILQAQGEKAFCAGGDLQQLY 79 GI TL + H A+S EM+ L QL ++D V VIL A G KAFCAG DL+Q+ Sbjct: 16 GITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANG-KAFCAGHDLRQM- 73 Query: 80 RSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTK---PILCWGHGIVMGGGIGL 136 A+P + Y A F +R +L+ S + P++ G+ G L Sbjct: 74 ------QATPQQ-----TYYEALF----HRCSHLMQSLRELPVPVIAKVQGLATAAGCQL 118 Query: 137 MAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKIGIFLALTGANLNAQDALFA 196 +A C + E +R A+ I +GL+ +P K + +TG ++A A Sbjct: 119 VATCDLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAW 178 Query: 197 GLADYGM 203 GL + + Sbjct: 179 GLINQAV 185 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 65.5 bits (158), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 51/192 (26%), Positives = 86/192 (44%), Gaps = 21/192 (10%) Query: 10 FEELTAANGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVILQAQGEKAF 69 +E + ++G+ L K L+A+S ++ L L + A E+ +I+ EKAF Sbjct: 3 YENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGS-EKAF 61 Query: 70 CAGGDLQQL--YRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKPILCWGHG 127 AG D+ + Y M + + EF + + + KP++ G Sbjct: 62 AAGADISAMAGYDYM--------------DVFKGEFITRNWET---LRRIRKPVIAAVAG 104 Query: 128 IVMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGKI-GIFLALTGA 186 +GGG L C + + ++ PEI +G+ P GG+ L R K + LALTG Sbjct: 105 YALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGR 164 Query: 187 NLNAQDALFAGL 198 ++A++A AGL Sbjct: 165 MMDAEEAERAGL 176 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 65.5 bits (158), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 56/193 (29%), Positives = 89/193 (46%), Gaps = 19/193 (9%) Query: 8 VVFEELTAA-NGRRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVILQAQGE 66 V +E+L AA +G + TL +A+ + ++ + L + D V +V++ E Sbjct: 5 VAYEDLLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGN-E 63 Query: 67 KAFCAGGDLQQLYRSMREHHASPAREDIRANRYAAEFFEQEYRLDYLIHSFTKPILCWGH 126 KAF AG DL + M A + D+RA + A I F KPIL + Sbjct: 64 KAFAAGADLNE----MIHKDAIATQLDVRAQYWRA------------IARFPKPILAAVN 107 Query: 127 GIVMGGGIGLMAGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARMPGK-IGIFLALTG 185 G +G G L+ + +++ PEI +G P GG+ L R GK + + + L+G Sbjct: 108 GYALGAGCELLMHADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSG 167 Query: 186 ANLNAQDALFAGL 198 ++A AL AGL Sbjct: 168 EFISADQALQAGL 180 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 61.6 bits (148), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 45/156 (28%), Positives = 74/156 (47%), Gaps = 14/156 (8%) Query: 19 RRIGIATLASEKTLHAISLEMVQLLTPQLQHWQADPEVAMVILQAQGEKAFCAGGDLQQL 78 R + TL + +A +++ L L + D +++L + G K+F AG DL + Sbjct: 12 RGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTG-KSFSAGADLAWM 70 Query: 79 YRSMREHHASPARED-IRANRYAAEFFEQEYRLDYLIHSFTKPILCWGHGIVMGGGIGLM 137 R A +RED +R R A E ++SF P L G VMGGG+GL+ Sbjct: 71 RR-----MADASREDNLRDARKLALLMET-------LNSFPTPTLAKVQGAVMGGGVGLV 118 Query: 138 AGCSHRVVTEKSRLAMPEITIGLYPDVGGSWFLARM 173 + C V ++ + A+ E+ +GL P + +A+M Sbjct: 119 SCCDIVVASDAAFFALSEVKLGLAPAAISPYVIAKM 154 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,259,390 Number of extensions: 51067 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 7 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)