BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0121.p (427 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 863 0.0 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 112 6e-26 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 105 8e-24 gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 97 3e-21 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 863 bits (2230), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/427 (99%), Positives = 427/427 (100%) Query: 1 VQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAVTILWLLDRTERLLSLVL 60 VQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAA+TILWLLDRTERLLSLVL Sbjct: 8 VQVLALFILILLSAFFSMTETALMAANRHRLRHLAKQGNRAAITILWLLDRTERLLSLVL 67 Query: 61 IANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIFAEIVPKVIGATYPERIS 120 IANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIFAEIVPKVIGATYPERIS Sbjct: 68 IANTAINALATALVTALAIAAFGNHQRVITAATACVAALLIIFAEIVPKVIGATYPERIS 127 Query: 121 LFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIVLEGGSF 180 LFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIVLEGGSF Sbjct: 128 LFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIVLEGGSF 187 Query: 181 IPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGE 240 IPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGE Sbjct: 188 IPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGE 247 Query: 241 INHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGV 300 INHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGV Sbjct: 248 INHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGV 307 Query: 301 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGACLLEGSTTLRDINKRL 360 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGACLLEGSTTLRDINKRL Sbjct: 308 VVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGACLLEGSTTLRDINKRL 367 Query: 361 GLQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMVKLIALAQAPG 420 GLQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMVKLIALAQAPG Sbjct: 368 GLQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMVKLIALAQAPG 427 Query: 421 AASAAPD 427 AASAAPD Sbjct: 428 AASAAPD 434 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 112 bits (280), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 105/437 (24%), Positives = 200/437 (45%), Gaps = 37/437 (8%) Query: 13 SAFFSMTETALMAANRHRLRHLAKQGNRAAVTILWLLDRTERLLSLVLIANTAINALATA 72 SAF S E ++ A+ + RL ++K+G A +L L + ++V I +NA+A Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFFTVVQIG---LNAVAI- 72 Query: 73 LVTALAIAAFGNHQRVITAATA---------------CVAALLIIFAEIVPKVIGATYPE 117 L + A H R I ++ V A I+FA+++PK + PE Sbjct: 73 LGGVVGEPALTPHMREIISSFYIGPWLENASFAMSFLVVTAFFILFADLMPKRLAMVAPE 132 Query: 118 RISLFTGFILKPLM---RVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEELRSIV 174 R ++ ++KP++ + KPL+WF N I L + + ++ I+P E+ ++V Sbjct: 133 RAAVA---LVKPMLLLETLFKPLVWFFNSLSKWIFTLFGL---PQVRQEVITPNEIHAMV 186 Query: 175 LEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYH 231 G G + Q+H ++ N+F+LE V MTPR + L I ++ H Sbjct: 187 DAGAQAGVLLTQEHH-LIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPH 245 Query: 232 NKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYF 291 ++ V +G I+ ++G + + + + E+ S D + L+ P +P +++ L+ Sbjct: 246 SRFLVCDGHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERM 304 Query: 292 QENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDKDGACLLEGST 351 + E L +VV+EY V G+++ D+ ++G + + + + L++G T Sbjct: 305 KAMDEDLAIVVNEYALVVGIISTTDVTGVLMGNLLAIT--EEEQQIVRRDENSWLMDGLT 362 Query: 352 TLRDINKRLGL-QLPLD-GPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRM 409 L D+ L + + P G +T++G ++ L+ IP+ + V E+V ++ I Sbjct: 363 PLNDVMSELDIDEYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRINQ 422 Query: 410 VKLIALAQAPGAASAAP 426 + + Q AAP Sbjct: 423 LLVTRFKQDKSEKDAAP 439 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 105 bits (261), Expect = 8e-24, Method: Compositional matrix adjust. Identities = 62/240 (25%), Positives = 125/240 (52%), Gaps = 9/240 (3%) Query: 187 SILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQLATCYHNKLPVFEGEINHIVG 246 S++ +F + ++V D+M PRSQ++ ++++ P++ + ++ H++ P +GE + +VG Sbjct: 55 SMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEVLATSHSRFPAVDGERDKVVG 114 Query: 247 ILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFTQLQYFQENHERLGVVVDEYG 306 IL + K + + EE D+R++L ++P + L+ F+ N + VVVDEYG Sbjct: 115 IL-LAKDLLRYYAEESF---DVRDMLRPAIFIPESKRLNVLLRDFRANRNHMAVVVDEYG 170 Query: 307 EVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWD-KDGA----CLLEGSTTLRDINKRLG 361 V GL+T+ED++E+++G+ E KDG ++ T + N+ +G Sbjct: 171 GVAGLITIEDVLEQIVGDIEDEYDFDEAEDNVLALKDGPHGARWRVKALTEIEQFNEEVG 230 Query: 362 LQLPLDGPKTINGLLLEYLQDIPEASVAVKMDGCVIEIVQVQNQAIRMVKLIALAQAPGA 421 + D TI GL+ ++ +P +DG E+++ + + ++ + L AP + Sbjct: 231 SRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLHFEVLRADARQVHVLLVSQLPPAPAS 290 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 96.7 bits (239), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 92/433 (21%), Positives = 192/433 (44%), Gaps = 31/433 (7%) Query: 3 VLALFILILLSAFFSMTETALMAANRHRLRHLAK-QGNRAAVTILWLLDRTERLLSLVLI 61 ++ F L+ L+ FF E +L+ + R+R +AK QG R + + + ++ + LS + Sbjct: 7 IVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAV-VHNQLDAYLSACQL 65 Query: 62 ANTAIN---------ALATALVTALAIAAFGN----HQRVITAATACVAALLIIFAEIVP 108 T + A A L ++A N H A ++ L I+ E+ P Sbjct: 66 GITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVAGELAP 125 Query: 109 KVIGATYPERISLFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPE 168 K + PE++ L+ L PLIW +N + +LR+ + G + S + Sbjct: 126 KSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAG-HGHDAHYSSD 184 Query: 169 ELRSIVLEG------GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDI 222 EL+ I+ G G F + ++L + ++ V D+M P S++ AL +++ Sbjct: 185 ELKLILRAGNKSGKNGKFT-RDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSLEEN 243 Query: 223 LHQLATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVET 282 L + +++ P ++ E ++G++H++ +++ + ++++ L Y+ Sbjct: 244 LDIIYRNRYSRYPYYDAERQQVLGLVHLKDV--FLAQQDGRAIANLKDYLRPVQYISPAL 301 Query: 283 DVFTQLQYFQENHERLGVVVDEYGEVQGLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDK- 341 L+ F+ V+ + G +TL++++ ++GE ++GE W + Sbjct: 302 PALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGE---WTRQ 358 Query: 342 -DGACLLEGSTTLRDINKRLGLQLPLDGP-KTINGLLLEYLQDIPEASVAVKMDGCVIEI 399 DG L +GS + + LG+ + D ++ GL++E L D+P+ + + I Sbjct: 359 DDGTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVI 418 Query: 400 VQVQNQAIRMVKL 412 ++ I +VK+ Sbjct: 419 KRMSGPKIVLVKV 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.138 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,108,533 Number of extensions: 44037 Number of successful extensions: 122 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 4 Length of query: 427 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 339 Effective length of database: 1,203,190 Effective search space: 407881410 Effective search space used: 407881410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)