BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0105.p (269 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate A... 549 e-158 gi|300310818|ref|YP_003774910.1| nitrate/sulfonate/bicarbonate A... 215 3e-57 gi|300311841|ref|YP_003775933.1| nitrate/sulfonate/bicarbonate A... 212 2e-56 gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase ... 212 2e-56 gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Her... 205 3e-54 >gi|300309717|ref|YP_003773809.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 549 bits (1414), Expect = e-158, Method: Compositional matrix adjust. Identities = 268/269 (99%), Positives = 268/269 (99%) Query: 1 MNQTNASPRIRIQGVHKVFKTDDREVVALKDINLDIPDGQFVCLLGPSGCGKSTLLNAIA 60 MNQTNASPRIRIQGVHKVFKTDDREVVALKDINLDIPDGQFVCLLGPSGCGKSTLLNAIA Sbjct: 1 MNQTNASPRIRIQGVHKVFKTDDREVVALKDINLDIPDGQFVCLLGPSGCGKSTLLNAIA 60 Query: 61 GFALPSSGQILTDDKVVSEPGPDRGMVFQEYALFPWMTVEQNVAFGLEIKGTPKAEIHQR 120 GFALPSSGQILTDDKVVSEPGPDRGMVFQEYALFPWMTVEQNVAFGLEIKGTPKAEIHQR Sbjct: 61 GFALPSSGQILTDDKVVSEPGPDRGMVFQEYALFPWMTVEQNVAFGLEIKGTPKAEIHQR 120 Query: 121 VTQLLDKPGLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTRRNLQ 180 VTQLLDK GLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTRRNLQ Sbjct: 121 VTQLLDKLGLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTRRNLQ 180 Query: 181 DELLRIWDEFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVKRDMLVNLPRLRDPADPEF 240 DELLRIWDEFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVKRDMLVNLPRLRDPADPEF Sbjct: 181 DELLRIWDEFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVKRDMLVNLPRLRDPADPEF 240 Query: 241 NALKRELGQLVMEEQQRHHNAEMKAAAVD 269 NALKRELGQLVMEEQQRHHNAEMKAAAVD Sbjct: 241 NALKRELGQLVMEEQQRHHNAEMKAAAVD 269 >gi|300310818|ref|YP_003774910.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 265 Score = 215 bits (547), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 122/217 (56%), Positives = 144/217 (66%), Gaps = 3/217 (1%) Query: 29 LKDINLDIPDGQFVCLLGPSGCGKSTLLNAIAGFALPSSGQILTDDKVVSEPGPDRGMVF 88 + I+LDI G+FVCLLGPSGCGKSTLLNAIAGF SG+I D K V PG DRG+VF Sbjct: 22 MAGIDLDIRAGEFVCLLGPSGCGKSTLLNAIAGFQ-DVSGEISVDGKRVKGPGLDRGVVF 80 Query: 89 QEY-ALFPWMTVEQNVAFGLEIKGTPKAEIHQRVTQLLDKPGLIDFRTRFPKDLSGGMRQ 147 Q ALFPW++V +NV +GL+++G AE + + GL +FP +LSGGMRQ Sbjct: 81 QSSEALFPWLSVRENVEYGLKLRGVASAERRAAAERYVSLVGLKHAIDKFPAELSGGMRQ 140 Query: 148 RVAIARVLALDSPIMLMDEPFGALDALTRRNLQDELLRIWDEFRKTIVFVTHSIEEAIYL 207 R IARVL + ++LMDEPFGALDA TR LQ EL RIW TIVFVTH I EAI L Sbjct: 141 RAQIARVLVNEPSVVLMDEPFGALDAQTREVLQSELDRIWKSTGCTIVFVTHDIWEAILL 200 Query: 208 ADRIVVMTYRP-GTVKRDMLVNLPRLRDPADPEFNAL 243 ADR+V MT P +K V+LP RDPADP AL Sbjct: 201 ADRVVTMTAGPRAHIKTIEEVHLPHPRDPADPACMAL 237 >gi|300311841|ref|YP_003775933.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 212 bits (540), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 109/220 (49%), Positives = 144/220 (65%), Gaps = 2/220 (0%) Query: 8 PRIRIQGVHKVFKTDDREVVALKDINLDIPDGQFVCLLGPSGCGKSTLLNAIAGFALPSS 67 P + I V +KT D V A + ++ ++ G LLGPSGCGKSTLL AI G+ P+S Sbjct: 16 PLLEIDRVTLQYKTTDSLVTATQSVSFNVHPGDRYILLGPSGCGKSTLLKAIGGYLQPTS 75 Query: 68 GQILTDDKVVSEPGPDRGMVFQEY-ALFPWMTVEQNVAFGLEIKGTPKAEIH-QRVTQLL 125 G I + V EPGPDR MVFQE+ L PW TV QNV F L G + +R + Sbjct: 76 GAIRLKGREVHEPGPDRMMVFQEFDQLLPWKTVRQNVEFALHASGRLQGRAAAERAEYYI 135 Query: 126 DKPGLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTRRNLQDELLR 185 K GL F +P LSGGM+QRV+IAR +A++ ++LMDEPF ALDALTRR +QDELLR Sbjct: 136 SKVGLAKFIDSYPHMLSGGMKQRVSIARGMAMEPDVLLMDEPFAALDALTRRKMQDELLR 195 Query: 186 IWDEFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVKRDM 225 +WD+ + T++FVTHSIEEAI + RI+++T PG V+ ++ Sbjct: 196 LWDDTQFTVLFVTHSIEEAIRVGTRILLLTPHPGQVRAEL 235 >gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 212 bits (540), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 115/256 (44%), Positives = 161/256 (62%), Gaps = 4/256 (1%) Query: 10 IRIQGVHKVFKTDDREVV-ALKDINLDIPDGQFVCLLGPSGCGKSTLLNAIAGFALPSSG 68 ++++ F D++ V AL ++LDIP+G FV LG SGCGKSTLLNA+AGF S G Sbjct: 2 LKVENASVYFSGRDKQRVHALDRVSLDIPNGGFVVALGTSGCGKSTLLNAMAGFLPLSEG 61 Query: 69 QILTDDKVVSEPGPDRGMVFQEYALFPWMTVEQNVAFGLEIKGTPKAEIHQRVTQLLDKP 128 +I D + VS PG +RG+VFQ+ +L PW +V+ NVA GL+ G K++ +R +LL Sbjct: 62 RISLDGQPVSGPGAERGVVFQKDSLLPWKSVQDNVALGLKFAGVSKSDSRERAQELLRLV 121 Query: 129 GLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTRRNLQDELLRIWD 188 GL DF P +LSGGMRQRV +AR LA + I+LMDEPFGALD++TR +Q+ L+ +W Sbjct: 122 GLQDFARAAPYELSGGMRQRVGLARALAPNPKILLMDEPFGALDSMTREQMQELLVSVWA 181 Query: 189 EFRKTIVFVTHSIEEAIYLADRIVVMTYRPGTVKRDMLVNLPRLRDPADPEFNALKRELG 248 K + F+THSIEEA++L ++VM+ RPG + + R R A E A+K E Sbjct: 182 RTGKQVFFITHSIEEALFLGTEVIVMSPRPGRIVARFETDFVR-RFAAGCEARAIKTEPA 240 Query: 249 QLVMEEQQRH--HNAE 262 + E+ R H +E Sbjct: 241 FAELREEIRAIVHQSE 256 >gi|300310542|ref|YP_003774634.1| nitrate transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 272 Score = 205 bits (521), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 112/264 (42%), Positives = 162/264 (61%), Gaps = 9/264 (3%) Query: 1 MNQTNASPR-IRIQGVHKVFKTDDREVVALKDINLDIPDGQFVCLLGPSGCGKSTLLNAI 59 MN N + + I I GV +F T AL++INL + G+FV L+G SGCGKSTLLN I Sbjct: 1 MNMDNINGKFIDIHGVEMLFHTKKGVFHALREINLTVAKGEFVTLIGHSGCGKSTLLNLI 60 Query: 60 AGFALPSSGQILTDDKVVSEPGPDRGMVFQEYALFPWMTVEQNVAFGLE-IKGTP--KAE 116 AG PS G +L ++ + P P+R +VFQ ++L PW++ +N+ G+E + G + + Sbjct: 61 AGLLKPSEGVLLCANREIGGPAPERAVVFQNHSLLPWLSCYENIYLGVERVFGATENRTQ 120 Query: 117 IHQRVTQLLDKPGLIDFRTRFPKDLSGGMRQRVAIARVLALDSPIMLMDEPFGALDALTR 176 + R L GL + P ++SGGM+QRV IAR LA++ ++LMDEPFGALDALTR Sbjct: 121 LRDRTRAALALVGLSAAENKRPNEISGGMKQRVGIARALAMEPKVLLMDEPFGALDALTR 180 Query: 177 RNLQDELLRIWDEFRKTIVFVTHSIEEAIYLADRIVVMTYRP-GTVKRDMLVNLPRLRD- 234 +LQDELL+I + T+V VTH ++EA+ L+DRIV+MT P T+ + V L R RD Sbjct: 181 AHLQDELLKIVAKTDSTVVMVTHDVDEAVLLSDRIVMMTNGPAATIGEVLEVKLARPRDR 240 Query: 235 ---PADPEFNALKRELGQLVMEEQ 255 DPE+ + + + + +Q Sbjct: 241 VVLAQDPEYGQYRTAVLEFLYRKQ 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 860,770 Number of extensions: 36415 Number of successful extensions: 503 Number of sequences better than 1.0e-08: 129 Number of HSP's gapped: 313 Number of HSP's successfully gapped: 136 Length of query: 269 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 185 Effective length of database: 1,222,130 Effective search space: 226094050 Effective search space used: 226094050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)