BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0055.p (936 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspi... 1932 0.0 gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herb... 181 2e-46 gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbasp... 84 4e-17 >gi|300309767|ref|YP_003773859.1| valyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 971 Score = 1932 bits (5006), Expect = 0.0, Method: Compositional matrix adjust. Identities = 933/936 (99%), Positives = 934/936 (99%) Query: 1 MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI 60 MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI Sbjct: 36 MELAKSFEPAEIEKFWREEWEKRGYFAATTDAEKPSFSIQLPPPNVTGTLHMGHAFNQTI 95 Query: 61 MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE 120 MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE Sbjct: 96 MDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKE 155 Query: 121 KSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVL 180 KSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVL Sbjct: 156 KSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVL 215 Query: 181 GTAVSDLEVVSEEEDGSMWHIKYPLADGSGHIVVATTRPETMLGDVAVAVDPTDERYAAL 240 GTAVSDLEVVSEEEDGSMWHIKYPLADGSGHIVVATTRPETMLGDVAVAVDPTDERYAAL Sbjct: 216 GTAVSDLEVVSEEEDGSMWHIKYPLADGSGHIVVATTRPETMLGDVAVAVDPTDERYAAL 275 Query: 241 VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA 300 VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA Sbjct: 276 VGKMIKLPLVGREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKA 335 Query: 301 TINENAPEKYQGLDRFEARKQIVADLDALGLLEQVKPHKLMVPRGDRTGVVIEPMLTDQW 360 TINENAPEKYQGLDRFEARKQIVADLDALGLLEQVKPHKLMVPRGDRTGVVIEPMLTDQW Sbjct: 336 TINENAPEKYQGLDRFEARKQIVADLDALGLLEQVKPHKLMVPRGDRTGVVIEPMLTDQW 395 Query: 361 FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW 420 FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW Sbjct: 396 FVAMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLW 455 Query: 421 WGHQIPAWYDEDGGIYVARTEEEARAQAAGRAVKRDEDVLDTWFSSALVPFSTLGWPEET 480 WGHQIPAWYDEDGGIYVARTEEEARAQA GRAVKRDEDVLDTWFSSALVPFSTLGWPEET Sbjct: 456 WGHQIPAWYDEDGGIYVARTEEEARAQAGGRAVKRDEDVLDTWFSSALVPFSTLGWPEET 515 Query: 481 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK 540 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK Sbjct: 516 PDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFDTVYVHGLVRDASGQKMSKSK 575 Query: 541 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFAEGIPAFGTDALRFTFA 600 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFA+GIPAFGTDALRFTFA Sbjct: 576 GNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATRKEFADGIPAFGTDALRFTFA 635 Query: 601 SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHVKGVCDKEKLQFS 660 SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGH KGVCDKEKLQFS Sbjct: 636 SLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQDCGFDGHEKGVCDKEKLQFS 695 Query: 661 KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ 720 KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ Sbjct: 696 KADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDEYCDWYLEMAKAQIQSGNEAQ 755 Query: 721 QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPLTDRKPDPAGDSIMRQAYPRPDLE 780 QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPLTDRKPDPAGDSIMRQAYPRPDLE Sbjct: 756 QRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPLTDRKPDPAGDSIMRQAYPRPDLE 815 Query: 781 KIDAEAEAWVAQFKAYTDACRNLRGEMQLAPSLRVPLIVEAADASAVESFLPYLQGLVRL 840 KIDAEAEAWVAQFKAYTDACRNLRGEMQLAPSLRVPLIVEAADASAVESFLPYLQGLVRL Sbjct: 816 KIDAEAEAWVAQFKAYTDACRNLRGEMQLAPSLRVPLIVEAADASAVESFLPYLQGLVRL 875 Query: 841 SETTVVAQLPESPAPVAIVGEVKLMLKVEIDVAAERERLGKEVTRLTAEIAKANGKLSNE 900 SETTVVAQLPESPAPVAIVGEVKLMLKVEIDVAAERERLGKEVTRLTAEIAKANGKLSNE Sbjct: 876 SETTVVAQLPESPAPVAIVGEVKLMLKVEIDVAAERERLGKEVTRLTAEIAKANGKLSNE 935 Query: 901 SFVARAPEAVVAQEKERVANFSATLEKVKEQLAKLK 936 SFVARAPEAVVAQEKERVANFSATLEKVKEQLAKLK Sbjct: 936 SFVARAPEAVVAQEKERVANFSATLEKVKEQLAKLK 971 >gi|300310736|ref|YP_003774828.1| isoleucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 964 Score = 181 bits (460), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 211/922 (22%), Positives = 368/922 (39%), Gaps = 170/922 (18%) Query: 10 AEIEKFWREEWEKRGYFAATTDAE--KPSFSIQLPPPNVTGTLHMGHAFNQTIMDGLTRY 67 A+ E W ++W+++ + A +P F + PP G +H+GHA N+ + D + + Sbjct: 39 AKREPQWVKQWQEKKVYERIRKASAGRPKFILHDGPPYANGEIHLGHAVNKILKDMIVKA 98 Query: 68 HRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVWEWKEKSGSTIT 127 M G++ ++PG D G+ +I +E++ +L + K + + + Sbjct: 99 RNMAGFDAQYVPGWDCHGMPIEIQIEKKYGK------NLPVAEVQAKARAYANEQIALQK 152 Query: 128 GQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNWDPVLGTAVSDL 187 R+G +W Y TM+ ++ E+G +YRG + VNW G+A+++ Sbjct: 153 KDFIRLGVLGEWDNPYKTMNFSNEADELRALGKILEKGYVYRGLKPVNWCFDCGSALAEA 212 Query: 188 EVVSEEEDGSMWHIKYPLADGS---------------GHIVVATTRPETMLGDVAVAVDP 232 EV +++ + +P A+ G+IV+ TT P T+ + A+ V P Sbjct: 213 EVEYQDKRDPAIDVGFPFAEHDKVATAFGLTDLPTRHGYIVIWTTTPWTIPANQALNVHP 272 Query: 233 TDERYA---------------------------ALVGKMI------KLPLV--------- 250 E YA L GK+I KL L+ Sbjct: 273 EFE-YALVETSRNGEATLLILAKDLVASCLERFGLEGKIIATTTGEKLSLIRFHHPLAKA 331 Query: 251 ----GREIPIIKDEYVDKEFGTGCVKITPAHDFNDYQVGARHNLEMISVFTLKATINENA 306 R P+ EYV + GTG V +PA+ D+ H ++ + A Sbjct: 332 DAGYDRLSPVFLGEYVTADSGTGIVHSSPAYGIEDFVSCKAHGMKDDDILNPVMGDGRYA 391 Query: 307 P--EKYQGLDRFEARKQIVADLDALGLLEQVK--PHKLMVPRGDRTGVVIEPMLTDQWFV 362 + GL +EA K I L+ +G L ++ H M ++ ++ T QWF Sbjct: 392 SWLPLFAGLTIWEASKPICDKLEEIGSLFKLIMFSHSYMHCWRHKSPIIYRA--TSQWFA 449 Query: 363 AMSKPAPEGTFFPGKSIAETALEKVSSGEIKFVPENWTNTYNQWLNNIQDWCISRQLWWG 422 +M +G G S+ +TAL+ + + F P + + N DW +SRQ WG Sbjct: 450 SMDNTPKDG----GPSLRQTALQGIE--DTAFFPGWGKARLHGMIANRPDWTLSRQRQWG 503 Query: 423 HQIPAWYDEDGGIYVARTEE---------EARAQAAGRAVK-------------RDEDVL 460 + + ++ G RT E E A +A+ +++D L Sbjct: 504 VPMAFFVHKETGELHPRTPELLEQIAQRIEQGGIEAWQALDPKELLGDEADQYLKNKDTL 563 Query: 461 DTWFSSALVPFSTLGWPEETPDYKLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGKVPFD 520 D WF S + L + +L P+ + + G D W ++ ++ G+ P+ Sbjct: 564 DVWFDSGSTHQTVL---RGSHRQELQFPADLYLEGSDQHRGWFHSSLLTSSMLNGRAPYK 620 Query: 521 TVYVHGLVRDASGQKMSKSKGNTLDPIDLIDGIGVDELVAKRTVGLMNPKQAASIEKATR 580 + HG V D G+KMSKS N ++P + D +G + + + V + SI + Sbjct: 621 ALLTHGFVVDGEGRKMSKSLKNGVEPQKVADSLGAE--ILRLWVASSDYSGEISISEEIL 678 Query: 581 KEFAEGIPAFGTDALRFTFASLATLGRNINFDLNRCEGYRNFCNKLWNATRFVLMNTEGQ 640 K R T E YR N L RF+L NT Sbjct: 679 K--------------RVT------------------EAYRRIRNTL----RFLLANTS-- 700 Query: 641 DCGFDGHVKGVCDKEKLQFSKADRWIVSLLQRTEAEVEKGFADYRFDNIATAIYKFVWDE 700 FD V E L+ DR+ ++ + R + EV F Y F + + + ++ Sbjct: 701 --DFDPAQHAVPMAELLEI---DRYAIANMARLQGEVLAHFETYEFHPVVAKLQMYCSED 755 Query: 701 YCDWYLEMAKAQIQSG--NEAQQRATRRTLLRVLESVLRLAHPVLPFITEQLWQSVAPLT 758 +YL++ K ++ + + A +R+ + + + +S+LRL P+L F E+ W A Sbjct: 756 LGGFYLDILKDRLYTSGVDSAARRSAQTAVWHITQSLLRLMAPILSFTAEEAWSFFA--G 813 Query: 759 DRKPDPAGDSIMRQA-YPRPDLEKIDAEAEAWVAQF-------KAYTDACRNLRGEMQLA 810 + +G++I Q Y P++ A+A+ +A+F A T +R + Sbjct: 814 KEAFEASGETIFSQVYYALPEV----ADAQGLLAKFAVLHEVRAAVTKQLEEVRIAGSIG 869 Query: 811 PSLR--VPLIVEAADASAVESF 830 SL+ V L V A+ +ES Sbjct: 870 SSLQAEVELKVAGEKAALLESL 891 >gi|300309930|ref|YP_003774022.1| leucyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 882 Score = 84.3 bits (207), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 58/203 (28%), Positives = 90/203 (44%), Gaps = 26/203 (12%) Query: 3 LAKSFEPAEIEKFWREEWEKRGYFAATTDA------EKPSFSIQLPPPNVTGTLHMGHAF 56 + + PAE+E+ ++ W + +A +K F P +G LHMGH Sbjct: 1 MQDKYSPAEVEQSAQQHWNAIDAYKTVENARDKQGRDKKKFYACSMLPYPSGKLHMGHVR 60 Query: 57 NQTIMDGLTRYHRMRGYNTAWIPGTDHAGIATQIVVERQLDAQKVSRHDLGREKFVEKVW 116 N TI D + RY RM GYN G D G+ E A V Sbjct: 61 NYTINDVMYRYLRMNGYNVLMPMGWDAFGMPA----ENAAMANGVPPA------------ 104 Query: 117 EWKEKSGSTITGQMRRMGASTDWAREYFTMDPKMSKVVTEVFVRLYEQGLIYRGKRLVNW 176 +W + + QM+ MG + DW+RE P+ K +F+++ E+G+IY+ VNW Sbjct: 105 QWTYSNIDYMKSQMQSMGLAIDWSREMTACRPEYYKWNQWMFLKMLEKGIIYKKTGTVNW 164 Query: 177 DPVLGTAVSDLEVVSEEEDGSMW 199 DP+ T +++ +V+ DG W Sbjct: 165 DPIDQTVLANEQVI----DGRGW 183 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,836,396 Number of extensions: 116700 Number of successful extensions: 329 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 324 Number of HSP's successfully gapped: 4 Length of query: 936 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 842 Effective length of database: 1,174,780 Effective search space: 989164760 Effective search space used: 989164760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)