BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0029.p (490 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) ... 983 0.0 gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirill... 509 e-145 gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum s... 146 4e-36 gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum... 125 8e-30 gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herb... 91 2e-19 >gi|300309787|ref|YP_003773879.1| sugar (pentulose and hexulose) kinase [Herbaspirillum seropedicae SmR1] Length = 490 Score = 983 bits (2540), Expect = 0.0, Method: Compositional matrix adjust. Identities = 489/490 (99%), Positives = 489/490 (99%) Query: 1 MYLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQAACDTVAKLR 60 MYLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQA CDTVAKLR Sbjct: 1 MYLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQATCDTVAKLR 60 Query: 61 TQLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESRAPRLHG 120 TQLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESRAPRLHG Sbjct: 61 TQLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESRAPRLHG 120 Query: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL Sbjct: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 Query: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAA 240 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAA Sbjct: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAA 240 Query: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS 300 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS Sbjct: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAAS 300 Query: 301 CLRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGLTPE 360 CLRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGLTPE Sbjct: 301 CLRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGLTPE 360 Query: 361 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV 420 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV Sbjct: 361 HQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIV 420 Query: 421 THVGSEAGGALGAARLAWLADGGDEAEVCRKPAQQALYQPDPARHAALQARLQRYRAIYA 480 THVGSEAGGALGAARLAWLADGGDEAEVCRKPAQQALYQPDPARHAALQARLQRYRAIYA Sbjct: 421 THVGSEAGGALGAARLAWLADGGDEAEVCRKPAQQALYQPDPARHAALQARLQRYRAIYA 480 Query: 481 PFAPLHGEGH 490 PFAPLHGEGH Sbjct: 481 PFAPLHGEGH 490 >gi|300312696|ref|YP_003776788.1| D-xylulose kinase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 509 bits (1312), Expect = e-145, Method: Compositional matrix adjust. Identities = 266/483 (55%), Positives = 326/483 (67%), Gaps = 2/483 (0%) Query: 2 YLGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQAACDTVAKLRT 61 YLGIDLGTSEVK V+ D +++A + L V PHP WSEQAP WW A D +A LRT Sbjct: 3 YLGIDLGTSEVKLVLTDEDSNVIASTSARLRVDNPHPLWSEQAPQSWWNATLDAIAALRT 62 Query: 62 QLGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSAPECAELESRAPRLHGI 121 Q + F ++R IGLSGQMHGA LLD VLRPAILW+D R+ EC ELE+ P I Sbjct: 63 Q-APQAFQALRGIGLSGQMHGATLLDRNGNVLRPAILWNDMRAHAECVELEALVPDAVAI 121 Query: 122 AGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWLD 181 GN AMPGFTAPKLLW++++EP ++ I VLLPKD+L K+TG VS+ SDAAGTLWLD Sbjct: 122 TGNRAMPGFTAPKLLWLSKYEPAVYRAIDKVLLPKDFLGWKLTGEFVSEMSDAAGTLWLD 181 Query: 182 VEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAAS 241 V R+WS+ +L A+G+ R MP LV+GSAV+G L E+ + WG+ V+VAGGAGD AAS Sbjct: 182 VARRDWSERMLGATGLDRSHMPRLVEGSAVAGQLRDELRREWGISGPVVVAGGAGDNAAS 241 Query: 242 AVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNPGRAIHAFCHTLPQRWHQMSVMLSAASC 301 AVGIG ++ GD FLSLG+SGVLF PN + +HAFCH LP +WHQMSV+LSAA+ Sbjct: 242 AVGIGVIRAGDAFLSLGSSGVLFAATAHHAPNAQQGVHAFCHCLPGQWHQMSVILSAAAS 301 Query: 302 LRWFCRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHNDPYATGVFHGLTPEH 361 L W + + L++ E+L APLFLPYL+GERTPHND A GV G+TP H Sbjct: 302 LHWLSGVTARPVAELVSGAERLSAGQQAQAPLFLPYLNGERTPHNDAAAKGVLFGMTPAH 361 Query: 362 QRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIVT 421 + A L YAV+EGVAF M DG AL+AAGT + + VGGG+RSA+W L A AL + + Sbjct: 362 ESAHLAYAVMEGVAFAMADGYAALQAAGTTLESAAFVGGGSRSAFWGVLCATALGIPLRR 421 Query: 422 HVGSEAGGALGAARLAWLA-DGGDEAEVCRKPAQQALYQPDPARHAALQARLQRYRAIYA 480 H G+E G ALGAARL LA G D A VC P Y+PD AR A LQ RL +YR +Y Sbjct: 422 HAGAEVGAALGAARLGRLARTGEDAASVCIPPEVLEWYRPDAARQAQLQRRLAQYRRLYR 481 Query: 481 PFA 483 A Sbjct: 482 SLA 484 >gi|300310425|ref|YP_003774517.1| gluconokinase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 146 bits (368), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 134/452 (29%), Positives = 199/452 (44%), Gaps = 22/452 (4%) Query: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQAACDTVAKLRTQ 62 LG+D+GT+ K+VV G +VA V P +EQ P AA ++A Sbjct: 11 LGVDIGTTSTKSVVFTLDGKVVAQHAEEYPVLCTEPGMAEQDPEQIVAAALASIAGAVKA 70 Query: 63 LGSERFGSIRAIGLSGQMHGAVLLDERDEVLRPAILWSDSRSA--PECAELESRAPRLHG 120 + I + S MH + LD + +L +I W D R++ E + E ++ Sbjct: 71 AKAAP-QEIALLSFSAAMHSVIALDAENRLLSNSITWGDIRASVWAERIKHEHDGNAIYR 129 Query: 121 IAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAGTLWL 180 G P KL+W+ +P +F R A + K++L ++ G+ V D S A+ T Sbjct: 130 RTGTPVHPMSPLCKLMWMRHEKPDVFHRAARFVGIKEYLLYQLFGQWVVDHSIASATGMF 189 Query: 181 DVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVIVAGGAGDGAA 240 ++ W LA G+R DQ+P V + +L E+AQ GL D GA DG Sbjct: 190 NLRELAWDQGALALLGVRPDQLPTPVPTTHRLPALSSEMAQRLGLSVDTPFIIGANDGVL 249 Query: 241 SAVGIGAVKPGDGFLSLGTSGVLFVVNDRFRPNP-GRAIHAFCHTLPQR-WHQMSVMLSA 298 S +G+ A++ G +++GTSG + V D R +P GR FC+ L ++ W + + Sbjct: 250 SNLGVNAIEIGHVAVTIGTSGAMRTVIDEPRTDPQGR---LFCYALTEKHWVVGGPVNNG 306 Query: 299 ASCLRWF-----------CRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGERTPHND 347 + RW R VD L I + LF P+++GER P + Sbjct: 307 GNIFRWVRDELATAEAAAAREEGVDPYEALTRIAEKVRPGAEGL-LFHPFMAGERAPLWN 365 Query: 348 PYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDALRAAGTDVAELSLVGGGARSAYW 407 G F GL H + + A LEGV F + L AL + GG ARSA W Sbjct: 366 ADLRGSFFGLALHHGKHHMIRAALEGVIFNLYSILPALEELVGPTKRMMATGGFARSALW 425 Query: 408 AQLLADALQVRIVTHVGSEAGGALGAARL-AW 438 Q++AD +V E+ LGAA L AW Sbjct: 426 RQMMADIFNREVVVPESVES-SCLGAAVLGAW 456 >gi|300310306|ref|YP_003774398.1| glycerol kinase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 125 bits (314), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 135/499 (27%), Positives = 220/499 (44%), Gaps = 42/499 (8%) Query: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWSEQAPADWWQAACDTVAKLRTQ 62 L +D GTS ++++ D G++VA A P P W E P D W + T K+ Q Sbjct: 5 LALDQGTSSSRSIIFDQSGAIVATAQQEFPQIFPQPGWVEHDPMDLWHSQLQTAQKVLAQ 64 Query: 63 LGSERFGSIRAIGLSGQMHGAVLLDE-RDEVLRPAILWSDSRSAPECAELESR--APRLH 119 G + + A+G++ Q V+ + + + A++W D R+ CA L R + Sbjct: 65 SGI-KASELTALGITNQRETTVVWERATGKPVYNAVVWQDRRAEVVCAALRERGLGEAIQ 123 Query: 120 GIAGNLAMPGFTAPKLLWVARHEPQLFAR-------IATVLLPKDWLRLKMTGRK--VSD 170 G + P F+ KL W+ H L AR TV WL +TG + VSD Sbjct: 124 KKTGLVLDPYFSGTKLRWILDHVDGLRARAERGELLFGTV---DSWLVWNLTGGRLHVSD 180 Query: 171 PSDAAGTLWLDVEGRNWSDELLAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSDVI 230 S+A+ T+ ++ W +ELL G+ +P + + + G + A L + V Sbjct: 181 VSNASRTMLWNIHTGAWDEELLQWLGIPAAMLPQVQPSAHLYGEI-----DAQHLGAAVK 235 Query: 231 VAGGAGDGAASAVGIGAVKPGDGFLSLGTSGVLFV-VNDRF-RPNPGRAIHAFCHTLPQR 288 + G AGD ++ G V+PG + GT + + D+ + G A C Q+ Sbjct: 236 IGGIAGDQQSALFGQACVRPGMAKNTYGTGCFMLLHTGDQCATSHNGLISTAACQVDEQK 295 Query: 289 WHQM--SVMLSAASCLRWF-----CRLCSVDEKSLLAEIEQLDEQACNNAPLFLPYLSGE 341 + + SV + A ++W S D + L +E + +F+P +G Sbjct: 296 QYALEGSVFIGGA-VVQWLRDGLKAIKTSTDVEGLACSVED------SGGVVFVPSFTGL 348 Query: 342 RTPHNDPYATGVFHGLTPEHQRAALGYAVLEGVAFGMVDGLDAL-RAAGTDVAELSLVGG 400 P+ +P A G GL+ A + A LE +AF L+A+ R A + + EL + GG Sbjct: 349 GAPYWNPSAKGAIVGLSRGSTVAHIARAALESIAFQSTALLNAMVRDAVSPITELRVDGG 408 Query: 401 GARSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLADG--GDEAEVCRKPAQQALY 458 A + Q AD L + ++ +E ALGAA LA +A G D +E+ + + + Sbjct: 409 AAANNLLLQFQADLLGIPVIRPQVTET-TALGAAYLAGIATGVYRDLSELSSQWRMEHTF 467 Query: 459 QPDPARHAALQARLQRYRA 477 +P R A QAR+ + A Sbjct: 468 EPSIGRDQA-QARIHAWEA 485 >gi|300310399|ref|YP_003774491.1| D-ribulose/ribitol kinase [Herbaspirillum seropedicae SmR1] Length = 548 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 139/544 (25%), Positives = 203/544 (37%), Gaps = 58/544 (10%) Query: 3 LGIDLGTSEVKAVVIDAQGSLVALAGSTLNVARPHPRWS-EQAPADWWQAACDTVAKLRT 61 +GID+GT +A V D QG L+A A +++ R R EQ+ A W+A C V T Sbjct: 6 IGIDVGTGSARAGVFDPQGQLLASARHDIDLFRDERRARVEQSSAQIWEAVCQAVRAAVT 65 Query: 62 QLGSERFGSIRAIGLSGQMHGAVL-----LDERDEVLRPAILWSDSRSAPECAELESRAP 116 + G + ++ IG+ V + + R I+W D R+ + + Sbjct: 66 KSGIDP-AAVAGIGVDATCSLVVQGASAGVGDPGHPERDVIVWMDHRALEQAQRINRMGH 124 Query: 117 RLHGIAGNLAMPGFTAPKLLWVARHEPQLFARIATVLLPKDWLRLKMTGRKVSDPSDAAG 176 + G + P PKLLW+ + P+++A A D+L K TG Sbjct: 125 AVLSYVGGVISPEMETPKLLWLKENLPEVYASAAHFFDLTDFLTWKATGSLQRSSCTVTC 184 Query: 177 TLWLDVEGRNWSDEL--------LAASGMRRDQMPALVDGSAVSGSLLPEVAQAWGLRSD 228 W LA +G R + G+ ++G L AQA GLR Sbjct: 185 KWTYLAHEAGWDASYFRQIGLGDLAEAGFARIGAEVVWPGTPLAGGLTQTAAQAMGLRPG 244 Query: 229 VIVAGGAGDGAASAVGIGAVKPGDG------FLSLGTSGVLFVVNDRFRPNPGRAIHAFC 282 + VA G D A VG A + G G GTS + PG + Sbjct: 245 IAVAAGLIDAHAGGVGTVAARGGQGDAAACMAYVFGTSSCTMTSHADPVLVPGVWGPYYK 304 Query: 283 HTLPQRWHQMSVMLSAASCLRWFCRLCSVD----EKSLLAEIEQ---LDEQACNNAP--- 332 P W +A + + RL E++ A++E L +QA Sbjct: 305 AMAPGMWLNEGGQSAAGAAIDHLLRLHPATPQAREEAARAQMELPQWLAQQALAAVDHPS 364 Query: 333 ---------LFLPYLSGERTPHNDPYATGVFHGLTPEH---QRAALGYAVLEGVAFGMVD 380 +P G R+P DP A GL EH AL A L + +G+ Sbjct: 365 EAVWLAGQLTVVPEFLGNRSPLADPQARAALVGLGMEHDIDSLVALYVAGLCSLGYGLRQ 424 Query: 381 GLDALRAAGTDVAELSLVGGGARSAYWAQLLADALQVRIVTHVGSEAGGALGAARLAWLA 440 ++A A G V +S+ GG QLLADA + + E LG+A LA +A Sbjct: 425 IIEAQAACGVRVQSISVSGGAGVHPLTRQLLADATGMPVEITASPEP-VLLGSAMLAAVA 483 Query: 441 DGGDE---------AEVCRK--PAQQALYQPDPARHAALQARLQRYRAIYAPFAPL---H 486 G + V + PAQ + AR+ A Q R + APL H Sbjct: 484 AGLHADLQSAMPAMSSVAGRSLPAQGDVAALHAARYQAFLKSQQLARELRDSLAPLLAAH 543 Query: 487 GEGH 490 +GH Sbjct: 544 TQGH 547 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,546,253 Number of extensions: 65029 Number of successful extensions: 235 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 5 Length of query: 490 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 401 Effective length of database: 1,198,455 Effective search space: 480580455 Effective search space used: 480580455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)