BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0018.p (339 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredox... 378 e-106 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 121 8e-29 gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredox... 120 1e-28 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 61 1e-10 >gi|300309798|ref|YP_003773890.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 339 Score = 378 bits (970), Expect = e-106, Method: Compositional matrix adjust. Identities = 189/190 (99%), Positives = 189/190 (99%) Query: 1 MNAVADRFNGSAATTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGL 60 MNAVADRFNGSAATTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGL Sbjct: 1 MNAVADRFNGSAATTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGL 60 Query: 61 VRAYSLCNTPQQRDCYLIAVKREPDSRGGSAFLHGQVQEGTTISASPPVNAFPLIADVRA 120 VRAYSLCNTPQQRDCYLIAVKREPDSRGGSAFLH QVQEGTTISASPPVNAFPLIADVRA Sbjct: 61 VRAYSLCNTPQQRDCYLIAVKREPDSRGGSAFLHEQVQEGTTISASPPVNAFPLIADVRA 120 Query: 121 PLLLAAGIGITPLHAMAAELATQGRRHRLHYFARSLAHAAFFHELAAGSQGMSLHLGLDG 180 PLLLAAGIGITPLHAMAAELATQGRRHRLHYFARSLAHAAFFHELAAGSQGMSLHLGLDG Sbjct: 121 PLLLAAGIGITPLHAMAAELATQGRRHRLHYFARSLAHAAFFHELAAGSQGMSLHLGLDG 180 Query: 181 AETEHAIAAA 190 AETEHAIAAA Sbjct: 181 AETEHAIAAA 190 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 121 bits (304), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 74/154 (48%), Positives = 96/154 (62%), Gaps = 1/154 (0%) Query: 14 TTLMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQR 73 TTL ++V A R+ A DI LELV+ DGAALP +T GAH+ + L GLVR YSLCN P ++ Sbjct: 2 TTLQVKVAARREEAHDIIALELVAVDGAALPAFTAGAHIDVHLPGGLVRQYSLCNAPHEQ 61 Query: 74 DCYLIAVKREPDSRGGSAFLHGQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPL 133 Y I V R+ +SRGGS +H ++ G ++ S P N F L+ + LLLA GIG+TP+ Sbjct: 62 HRYQIGVLRDVNSRGGSVAVHEALKLGDILTISAPRNQFALVP-AQHSLLLAGGIGVTPI 120 Query: 134 HAMAAELATQGRRHRLHYFARSLAHAAFFHELAA 167 MA LA G +HY ARS A AF +AA Sbjct: 121 LCMAEALAAAGASFEMHYCARSPARQAFRERIAA 154 >gi|300310671|ref|YP_003774763.1| flavodoxin reductases (ferredoxin-NADPH reductases) family 1 protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 77/191 (40%), Positives = 97/191 (50%), Gaps = 3/191 (1%) Query: 16 LMLRVRAVRQAAQDIRLLELVSQDGAALPPWTPGAHLRLQLQDGLVRAYSLCNTPQQRDC 75 + L V + DIR LV DGA LP + PGAH+ + G++R YSLC + R Sbjct: 1 MKLIVDTLTDETGDIRSFRLVRADGAPLPAYEPGAHIDVTGPTGIMRQYSLCGPLEDRCG 60 Query: 76 YLIAVKREPDSRGGSAFLHGQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHA 135 Y IAVKRE SRGGSA LH V+ G + P N F L A LL AGIG+TPL A Sbjct: 61 YTIAVKRESASRGGSAALHDVVKAGMELEVGTPRNLFALDASAPEHLLFGAGIGVTPLLA 120 Query: 136 MAAELATQGRRHRLHYFARSLAHAAF--FHELAAGSQGMSLHLGLDGAETEHAIAAAWRR 193 MA L + +R LHYF R+ HAAF E A + + H + A+ EH + Sbjct: 121 MAYRLWAEEKRFTLHYFVRAREHAAFADLLENAPFAAHVRFHYAVMPAQMEHYLTVCLEA 180 Query: 194 -RRVLHRSTCA 203 + H TC Sbjct: 181 GSKEAHVYTCG 191 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 51/150 (34%), Positives = 73/150 (48%), Gaps = 10/150 (6%) Query: 27 AQDIRLLELVSQDGAALPPWTPGAHLRLQLQ-DGLV--RAYSLCNTPQQRDC---YLIAV 80 +++I V QDG L + PG ++ L++ DG R YSL +Q Y I+V Sbjct: 167 SEEITSFHFVPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQPPQYRISV 226 Query: 81 KREPDSRGGSAFLHGQVQEGTTISASPPVNAFPLIADVRAPLLLAAGIGITPLHAMAAEL 140 KREP + S LH Q+ G+ + PP F L+A + L++ G+GITP+ +M A Sbjct: 227 KREPGGKV-SRQLHDQIHVGSVVELFPPAGEFTLMASDKPLALISGGVGITPMMSMLAAA 285 Query: 141 ATQGRRHRLHYFARSLAHAAF---FHELAA 167 GR + AR AF ELAA Sbjct: 286 LPSGRPIHFIHAARHGGVHAFRRQLEELAA 315 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.133 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 982,200 Number of extensions: 36166 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 179 Number of HSP's successfully gapped: 4 Length of query: 339 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 253 Effective length of database: 1,212,660 Effective search space: 306802980 Effective search space used: 306802980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 131 (55.1 bits)