BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0013.p (267 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate A... 519 e-148 gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 112 3e-26 gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate A... 81 1e-16 gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate A... 77 2e-15 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 68 8e-13 >gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 519 bits (1336), Expect = e-148, Method: Compositional matrix adjust. Identities = 267/267 (100%), Positives = 267/267 (100%) Query: 1 MKPNTDPLVNRPGFWRVAAPLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWS 60 MKPNTDPLVNRPGFWRVAAPLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWS Sbjct: 1 MKPNTDPLVNRPGFWRVAAPLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWS 60 Query: 61 MLMASLLVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLI 120 MLMASLLVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLI Sbjct: 61 MLMASLLVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLI 120 Query: 121 IIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPS 180 IIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPS Sbjct: 121 IIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPS 180 Query: 181 ALPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLIT 240 ALPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLIT Sbjct: 181 ALPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLIT 240 Query: 241 LTGILLFWLMSALSRAMLAGWHESEMA 267 LTGILLFWLMSALSRAMLAGWHESEMA Sbjct: 241 LTGILLFWLMSALSRAMLAGWHESEMA 267 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 112 bits (280), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 66/248 (26%), Positives = 129/248 (52%), Gaps = 6/248 (2%) Query: 19 APLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWSMLMASLLVTLKITFLAFA 78 AP + + +L +WQ +C++ +V ++ P+P I++ + + L T +T + FA Sbjct: 17 APWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPITEHALSTFWVTMVGFA 76 Query: 79 LAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLIIIWVKDTTAALTVCATIM 138 +AVV+GV + FV S + + +P P A P++++W + A ++ Sbjct: 77 IAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAFLI 136 Query: 139 ALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFFGGLRISSGLALI 198 + FPI+ N GL ++ P L ++ R+ A R L ++ +P ++PYFF L+++ LA + Sbjct: 137 SFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLAFV 196 Query: 199 GAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITLTGILLFWLMSALSRAML 258 G+ V+E A + G+ Y ++ AG + +P FA L++I + ++ L + + + Sbjct: 197 GSTVSEMNA----SNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAMYELFAIIEKRT- 251 Query: 259 AGW-HESE 265 GW H + Sbjct: 252 TGWAHRGK 259 >gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 80.9 bits (198), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 59/185 (31%), Positives = 92/185 (49%), Gaps = 4/185 (2%) Query: 62 LMASLLVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLII 121 + + LL+TL T LAF + V G+AI S L PY P V +AP+ Sbjct: 79 IYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRVILAPIFA 138 Query: 122 IWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSA 181 +W + A + F + N G++ V+P +L R+ A Q LR + +PSA Sbjct: 139 MWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLRTVYLPSA 198 Query: 182 LPYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITL 241 + F L S GLA +GAVV E++ G+ G+ Y ILQA +I +FA ++++T+ Sbjct: 199 TSWVFSSLHTSIGLAFVGAVVGEYL----GSARGVGYLILQAEGTFDINTVFAGIVVLTI 254 Query: 242 TGILL 246 ++L Sbjct: 255 FALVL 259 >gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 284 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 72/256 (28%), Positives = 121/256 (47%), Gaps = 18/256 (7%) Query: 16 RVAAPLIIG--IALLLM----WQIVCTLLEVPPYLVPTPALIVKTLAADWSM-------L 62 R L+IG IA+L++ W++ + + P+ P+LIV + DW + L Sbjct: 27 RARKQLVIGLRIAILVIVLGGWEVCARIGWIDPFFFSQPSLIVAQIY-DWMVEGTSQGPL 85 Query: 63 MASLLVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLIII 122 +LVTL+ T L F + V GV V +++ + Y I P V + + +I Sbjct: 86 WTQVLVTLEETVLGFLIGSVAGVIAGIVLGRNKLLSDVFSLYIQIANSIPRVVLGSIFVI 145 Query: 123 WVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSAL 182 A+ A +M F + +N G+R + ++ ++ A+R Q + IPSAL Sbjct: 146 AFGLGMASKVALAVVMVFFVVFANAFQGVREADRYMIANAQILGASRRQVTMAVVIPSAL 205 Query: 183 PYFFGGLRISSGLALIGAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAALLLITLT 242 + L +S G AL+GAVV EF+ G+ G+ I A N +FAA++++ + Sbjct: 206 SWILASLHVSFGFALVGAVVGEFL----GSKQGIGLLISTAQGAFNASGVFAAMIVLAVV 261 Query: 243 GILLFWLMSALSRAML 258 + WL+ AL R +L Sbjct: 262 ALAADWLLHALERRLL 277 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 67.8 bits (164), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 61/216 (28%), Positives = 98/216 (45%), Gaps = 13/216 (6%) Query: 3 PNTDPLVNRPGFWRVAAPLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWSM- 61 P+ D RPG + I+ +A LL+ + L +P L+P P+ + TL WS+ Sbjct: 16 PHDD--AYRPGRF---TGWIVPLAFLLLVEAGARLELIPARLLPPPSELAHTL---WSLA 67 Query: 62 ----LMASLLVTLKITFLAFALAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIA 117 L+ + +L F + L VA+ + SR E L P L+ P +A Sbjct: 68 QRGELLPHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWI 127 Query: 118 PLIIIWVKDTTAALTVCATIMALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLR 177 PL+++W+ V I A FP+ N G+R ++ L+ + + + + Sbjct: 128 PLLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHIL 187 Query: 178 IPSALPYFFGGLRISSGLALIGAVVAEFVAGTGGTG 213 +P++LPY F GLR LA + V AE +A T G G Sbjct: 188 LPASLPYLFAGLRGGLSLAWMFLVAAELIAATKGVG 223 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.142 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 690,652 Number of extensions: 24185 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 15 Length of query: 267 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 183 Effective length of database: 1,222,130 Effective search space: 223649790 Effective search space used: 223649790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)