BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0012.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 947 0.0 gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 258 5e-70 gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [H... 57 4e-09 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 947 bits (2448), Expect = 0.0, Method: Compositional matrix adjust. Identities = 467/471 (99%), Positives = 469/471 (99%) Query: 1 MSTFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALPGEELIDASHCVAYPA 60 MSTFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALPGEELIDASHCVAYPA Sbjct: 1 MSTFLIRNLQAIMTGLPGAAARHAGPDIRVSDGVIAAIGQLDALPGEELIDASHCVAYPA 60 Query: 61 WVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSGC 120 WVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSGC Sbjct: 61 WVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMRSGC 120 Query: 121 GTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELPSALRPETLD 180 GTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAEL SALRPETLD Sbjct: 121 GTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELSSALRPETLD 180 Query: 181 GFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREIAVAARAMGLRLHSHLSE 240 GFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELRE+AVAAR MGLRLHSHLSE Sbjct: 181 GFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREMAVAAREMGLRLHSHLSE 240 Query: 241 TVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSN 300 TVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSN Sbjct: 241 TVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSN 300 Query: 301 GRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRG 360 GRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRG Sbjct: 301 GRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRG 360 Query: 361 GHFEGGADAATVEDVVRWGSTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPA 420 GHFEGGADAATVEDVVRWG+TGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPA Sbjct: 361 GHFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPA 420 Query: 421 IGPVASGGRPFLKAMWVGGRAVVREDAIPGVDLAELGAQARQAVKKMLKSN 471 IGPVASGGRPFLKAMWVGGRAVVREDAIPGVDLAELGAQARQAVKKMLKSN Sbjct: 421 IGPVASGGRPFLKAMWVGGRAVVREDAIPGVDLAELGAQARQAVKKMLKSN 471 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 258 bits (660), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 166/457 (36%), Positives = 236/457 (51%), Gaps = 30/457 (6%) Query: 2 STFLIRNLQAIMTGLPGAAARHAGPD--IRVSDGVIAAIGQLDALP--GEELIDASHCVA 57 T LIRN + ++T +R PD + + + VI +GQ LP +E+IDA H + Sbjct: 8 KTLLIRNARILVTM---NESREEIPDGALYIRNNVIEQVGQTADLPQDADEIIDARHHIV 64 Query: 58 YPAWVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVELMR 117 P +NTHHH++QSL + P N L WL Y A ++ +++ M EL+ Sbjct: 65 LPGLINTHHHMYQSLTRAIPSAQNGELFNWLTKL-YPIWAGLTPEMIQVSTLTAMAELIL 123 Query: 118 SGCGTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELPSALRPE 177 SGC T +DH Y+Y D S E A ++G+RF RG A Q + LP + Sbjct: 124 SGCTTSSDHLYIYPNQTRLDDS---LEAAARIGMRFHGARG-AMSVGQSKGGLPPDRVVQ 179 Query: 178 TLDGFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREIAVAARAMGLRLHSH 237 L D Q L +HD +M+R+V+AP +P +S + + +RE A AR G+ LH+H Sbjct: 180 DEAAILKDTQRLIETYHDHQRHAMQRIVVAPCSP-FSVSRELMREAADLARHYGVSLHTH 238 Query: 238 LSETVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCP 297 L+E V + P E+ E GW+G DVW AH V+LDA I L TGTG+AHCP Sbjct: 239 LAENVNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQLDAHGIDLFARTGTGVAHCP 298 Query: 298 QSNGRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARRGQEATPS 357 SN RL SGIAPIR + AGVPV +GVDG+ASN++A M+ E A L+QR Sbjct: 299 CSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRV--------G 350 Query: 358 FRGGHFEGGADAATVEDVVRWGSTGGARVLGLDALDGLQVGQQADIALYRLDDPRYFG-L 416 F G DA T + + GGA+VL D + L+ G AD+ ++ + G L Sbjct: 351 F-------GPDAMTARQALEVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQIAFAGAL 403 Query: 417 HDPAIGPVASGGRPFLKAMWVGGRAVVREDAIPGVDL 453 HDP + + + + GR +VRE + ++L Sbjct: 404 HDP-VAALVFCTPANVDCSIINGRVIVREGQLTSIEL 439 >gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 104/431 (24%), Positives = 162/431 (37%), Gaps = 86/431 (19%) Query: 59 PAWVNTHHHLFQSLLKG--DPLGINA-TLTPWLAATPYRYRASFDADLFRLAARIGMVEL 115 P N H H FQ + G + +G A + W YR+ AR +E+ Sbjct: 71 PGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELM-YRFAQRLQPRQLEAIARQTYIEM 129 Query: 116 MRSGCGTIADHNYLYYP--GMPF-DTSEI---LFEEAQQLGLRFVLCRGTATRTRQLEAE 169 +++G ++ + +YL++ G P+ D +E+ L + A Q G+ L Sbjct: 130 LKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGMTL-------------- 175 Query: 170 LPSALRPETLDGFLADMQGLAGRFHDPAPASMRRVVMAPTTPL----------------- 212 LP L GL GR P A RR V AP+ L Sbjct: 176 LP----------VLYQQGGLGGR---PVSAEQRRFVAAPSWLLGLRERLLQHMPENDMRR 222 Query: 213 YSAAPDELREIAVAARAMGLR------------LHSHLSETVGYQDSAHAMHGCRPVEFC 260 Y AP LR +A L +H H++E + + A G RPV++ Sbjct: 223 YGVAPHSLRAVAPTGLEELLSGLAGQPGGSDAPIHIHVAEQLREVEDCLAWSGQRPVQWL 282 Query: 261 ERVGWLGRDVWFAHLVKLDAEEIQLLGGTGTGIAHCPQSNGRLGSGIAPIRALEAAGVPV 320 + H +D EE + + G+G + CP + LG G+ L + Sbjct: 283 LSSQPVDARWCLVHATHMDDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARW 342 Query: 321 SIGVDGAASNEAADMISETHAAWLMQRARRGQEATPSFRGGHFEGGADAATVEDVVRWGS 380 IG D SN A + +E L++ A+R R + DA V D + + Sbjct: 343 GIGSD---SNIAISLRAELR---LLEYAQRLHH-----RRRNVLAVIDAPAVADRLFCQA 391 Query: 381 TGGARVLGLDALDGLQVGQQADIALYRLDDPRYFGLHDPAIGPVASG------GRPFLKA 434 G A+ GL GQ+AD+ + DP + L + + S P + Sbjct: 392 LAGGAQAAGRAVAGLMPGQRADLVVL---DPDHVNLAQRSSTQLLSALVFCEHDGPMIGD 448 Query: 435 MWVGGRAVVRE 445 ++VGGR VV+E Sbjct: 449 VYVGGRQVVKE 459 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,727,868 Number of extensions: 82563 Number of successful extensions: 270 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 264 Number of HSP's successfully gapped: 3 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)