BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0008.p (332 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetas... 536 e-153 >gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetase [Herbaspirillum seropedicae SmR1] Length = 9160 Score = 536 bits (1380), Expect = e-153, Method: Compositional matrix adjust. Identities = 276/279 (98%), Positives = 277/279 (99%) Query: 1 MSRPPHEHAPLSYSQQQLWFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHA 60 MSRPPHEHAPLSYSQQQLWFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHA Sbjct: 5190 MSRPPHEHAPLSYSQQQLWFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHA 5249 Query: 61 ILRTRFAETDGLPYQQAVPPFPVQLAWLDLRQLPPQQREQRLQEELQAAAAHVFDLGQAG 120 ILRTRFAETDGLPYQQAVPPFPVQLAWLDLRQLPPQQREQRLQEELQAAAAHVFDLGQAG Sbjct: 5250 ILRTRFAETDGLPYQQAVPPFPVQLAWLDLRQLPPQQREQRLQEELQAAAAHVFDLGQAG 5309 Query: 121 QLDVRLIALGPQEHVLVIGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQAQLPALPMQYA 180 QLDVRLIALGPQEHVLVIGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQAQLPALPMQYA Sbjct: 5310 QLDVRLIALGPQEHVLVIGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQAQLPALPMQYA 5369 Query: 181 DYAHWQRQRVQGAWLADELRYWDDYLGPVNSSLDLPTDRPRPARQSFRGAVHAFTLDSAL 240 DYAHWQRQRVQGAWLADELRYWDDYLGPVNSSLDLPTDRPRPARQSFRGAVHAFTLDSAL Sbjct: 5370 DYAHWQRQRVQGAWLADELRYWDDYLGPVNSSLDLPTDRPRPARQSFRGAVHAFTLDSAL 5429 Query: 241 AQQMQAFCRAQHCTPFVALMAAWQVLLSRYSGQADFARG 279 AQQMQAFCRAQHCTPFVALMAAWQVLLSRYSGQ+DF G Sbjct: 5430 AQQMQAFCRAQHCTPFVALMAAWQVLLSRYSGQSDFLVG 5468 Score = 141 bits (356), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 98/267 (36%), Positives = 133/267 (49%), Gaps = 2/267 (0%) Query: 19 WFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHAILRTRFAE-TDGLPYQQA 77 W Q P ++ A L G +D L AL L ARH ILRT F E DG P Sbjct: 3270 WTACQARPMRPPTTMSAALMLEGVVDAVLLEQALRLLVARHDILRTGFGEDADGNPVA-T 3328 Query: 78 VPPFPVQLAWLDLRQLPPQQREQRLQEELQAAAAHVFDLGQAGQLDVRLIALGPQEHVLV 137 + PF L DL LPP QR+ +L ++ AA F L Q L L+ L Q+ VL+ Sbjct: 3329 LHPFDFSLEQHDLAALPPPQRDLQLDRLRRSHAAARFALRQPPLLRAALVRLASQQQVLL 3388 Query: 138 IGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQAQLPALPMQYADYAHWQRQRVQGAWLAD 197 + +HHIV+D WS L++ ++A AY + P LP+QYADYA WQ L D Sbjct: 3389 LAIHHIVADGWSVALMMEEIAAAYSALQEGRSYAPPPLPLQYADYAAWQVAGAGSRMLED 3448 Query: 198 ELRYWDDYLGPVNSSLDLPTDRPRPARQSFRGAVHAFTLDSALAQQMQAFCRAQHCTPFV 257 + +W D L V ++ LP+ RPRPAR ++ G L + L +QA R + T + Sbjct: 3449 SIAWWRDALAGVPATPYLPSTRPRPARATYAGQRLRMHLPAHLRSGVQALARREGVTAYA 3508 Query: 258 ALMAAWQVLLSRYSGQADFARGRGAVG 284 L+A++ LL + SG DF G G Sbjct: 3509 VLLASFAWLLHQRSGAGDFVVGTDVAG 3535 Score = 136 bits (343), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 104/275 (37%), Positives = 135/275 (49%), Gaps = 6/275 (2%) Query: 10 PLSYSQQQLWFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHAILRTRFAET 69 PL+ +QQ LW Q +P AYNL L G L AL AL L ARHA LRT F Sbjct: 692 PLTQAQQGLWLTWQLQPDSAAYNLAGVLDLRGELQVEALEDALCDLAARHAALRTLFPLR 751 Query: 70 DGLPYQQAVPPFPVQLAWL--DLRQLPPQQREQRLQEELQAAAAHVFDLGQAGQLDVRLI 127 DG P QQ + + L DLR+ P +R Q LQ A F L L L+ Sbjct: 752 DGTPVQQVLDIDEARPMLLRSDLRRQPADKRAQAAASALQRFHAQAFRLDSETPLRAMLV 811 Query: 128 ALGPQEHVLVIGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQA-QLPALPMQYADYAHWQ 186 L P L + +HHI +D WS +++ +L AY +AR G A PAL +Q DYA WQ Sbjct: 812 QLEPHHFRLGLCIHHIAADGWSLGIILDELTAAY-EARCAGAAPSWPALELQPGDYAVWQ 870 Query: 187 RQRVQ--GAWLADELRYWDDYLGPVNSSLDLPTDRPRPARQSFRGAVHAFTLDSALAQQM 244 RQR Q +L YW L + L LPTDRPR A H + L +AL+ + Sbjct: 871 RQRSQPGSPEFQRQLDYWRARLEGAPALLPLPTDRPRQAGAHGGEGQHRWRLSAALSTAL 930 Query: 245 QAFCRAQHCTPFVALMAAWQVLLSRYSGQADFARG 279 + +Q T + A++A + + L R SGQ+D G Sbjct: 931 RKLAASQGVTLYGAVLALFTLALQRLSGQSDLCVG 965 Score = 94.0 bits (232), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 92/287 (32%), Positives = 124/287 (43%), Gaps = 16/287 (5%) Query: 10 PLSYSQQQLWFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHAILRTRFAET 69 PLS Q + F +EPG +AY + G LD A A A H ILRT F Sbjct: 6743 PLSPMQAGMLFHSVFEPGGSAYINQLRVDIDG-LDAARFEQAWQDTVAAHDILRTGFLHQ 6801 Query: 70 DGLPYQQAVPPFPVQLAWLDLRQLPPQQREQRLQEELQAAA----AHVFDLGQAGQLDVR 125 P Q L+ LD R Q L EL A A A FDL + + Sbjct: 6802 P-RPLQWVARRVASPLSQLDWRD---AAAGQSLGGELDALAQRQLAQGFDLAAPPLMRLV 6857 Query: 126 LIALGPQEHVLVIGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQAQLPALPMQYADYAHW 185 L+ G Q H L+ +HH++ D WS +++ ++ Y QA GQA+ L ++Y DY W Sbjct: 6858 LVRTGAQRHHLIWTVHHLLLDGWSTSMLLGEVLRRYAQATAAGQAKPTKLALRYRDYIGW 6917 Query: 186 QRQRVQGAWLADELRYWDDYLGPVNSSLDLPTDRPRPARQSFRGAVHAFTLDSALAQQMQ 245 + R Q A R+W+ L + L PRP+ H LD+AL ++ Sbjct: 6918 LQGRDQEA----TRRFWEIQLARLQQPTRLADAMPRPSTPESGHGEHRELLDAALTARLN 6973 Query: 246 AFCRAQHCTPFVALMAAWQVLLSRYSGQADFARGRGAVGGAGCAAGP 292 RAQ T + AAW +LL RY+ A +G V GA A P Sbjct: 6974 EAARAQRITLNTLVQAAWALLLQRYTYA---AHDQGVVFGATVAGRP 7017 Score = 68.2 bits (165), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 84/285 (29%), Positives = 121/285 (42%), Gaps = 29/285 (10%) Query: 10 PLSYSQQQLWFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHAILRTRFA-E 68 PLS QQ + F PG Y + R+ G LD A + A ++ ARH ++RT F + Sbjct: 7815 PLSPMQQGMLFHTMESPGSGLYVNQLSVRVEG-LDEARFAQAWQSVLARHPVMRTGFLWQ 7873 Query: 69 TDGLPYQQAV---PPFPVQLA-WLDLRQLPPQQREQRLQEELQAAAAHVFDLGQAGQLDV 124 D QAV F +++ W +P +R AA + Sbjct: 7874 ADYAQPLQAVWRTLAFGIEVQDWSARATMPSCRRRLPPLRRPILRAAST--CCNRPLVRA 7931 Query: 125 RLIALGPQEHVLVIGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQAQLPALPMQYADYAH 184 L+ LGP +H LV MHHI+ D WS ++++L AY G LPA YA Y Sbjct: 7932 TLLRLGPGQHQLVWTMHHILLDGWSTSALMQELMLAY------GGQPLPAPGPDYAQYIG 7985 Query: 185 W----QRQRVQGAWLADELRYWDDYLGPVNS-SLDLPTDRPRPARQSFRGAVHAFTLDSA 239 W Q Q RYW L ++ SL RP PAR+ F G ++ D+A Sbjct: 7986 WLATLDPQAAQ--------RYWMGALQALDGPSLLAEGVRPDPARRGF-GKLYGHC-DAA 8035 Query: 240 LAQQMQAFCRAQHCTPFVALMAAWQVLLSRYSGQADFARGRGAVG 284 ++++ F R+Q T + AW + L+R+ A G G Sbjct: 8036 ATRRLEQFARSQRVTLNTLVQGAWSLALARHKRSTAVAFGATVSG 8080 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 89/289 (30%), Positives = 117/289 (40%), Gaps = 33/289 (11%) Query: 11 LSYSQQQLWFLQQYEPGLTAYNLTRAFRLTGALDRAALSAALDALQARHAILRTRFAETD 70 LS Q ++F G AY L G +D A L A RH ILRT F + Sbjct: 2191 LSPMQAGMFFHSSMNAGDGAYINQLRLDLAG-VDEARLEAVWREAVRRHPILRTGFRQQG 2249 Query: 71 GLPYQQAVPPFPVQLAW--LDLRQLPPQQREQRLQEELQ-----------AAAAHVFDLG 117 P Q P L W LDL PPQQ+ Q+ Q E A A FDL Sbjct: 2250 DAPEQWVAQPV---LHWRVLDLSGQPPQQQAQQAQLEQLEQLEQLEQIAAAELARGFDLA 2306 Query: 118 QAGQLDVRLIALGPQEHVLVIGMHHIVSDAWSNPLVIRDLAEAYRQARRDGQAQLPALPM 177 Q G + + L +G H L+ HH++ D WS+ + ++ AY ++ A + Sbjct: 2307 QPGLMRLVLAKMGEGRHHLIWTRHHLLLDGWSSAQLFAEIVRAY------AGEEIAAPTL 2360 Query: 178 QYADYAHWQRQRVQGAWLADELRYWDDYLGPVNSSLDLPTDRPRPARQSFRGAVHAFTLD 237 Y Y W + R GA D +W L + L PRP R+ G HA L Sbjct: 2361 AYRHYIDWLQAR-DGAAARD---FWRAQLQHLEEPTLLAGALPRP-REDASG--HAEHLA 2413 Query: 238 SALAQQMQAFC---RAQHCTPFVALMAAWQVLLSRYSGQADFARGRGAV 283 + Q QA C +A TP L AW ++L R GQ+ A G V Sbjct: 2414 AISPAQWQALCQAAKAMRITPGTLLQGAWALVLQRCCGQSAVAFGVTVV 2462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 875,512 Number of extensions: 31527 Number of successful extensions: 110 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 7 Length of query: 332 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 246 Effective length of database: 1,212,660 Effective search space: 298314360 Effective search space used: 298314360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 131 (55.1 bits)