BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0006.p (317 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum... 622 e-179 gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi... 151 8e-38 gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro... 98 1e-21 gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase fa... 72 5e-14 >gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum seropedicae SmR1] Length = 317 Score = 622 bits (1604), Expect = e-179, Method: Compositional matrix adjust. Identities = 313/317 (98%), Positives = 314/317 (99%) Query: 1 MPLHPDLDAFLALVEAGIQSGRQQPMHRKSVHQARADYEAAAPMLDLPPDDLAERRALEI 60 MPLHPDLDAFLALVEAGIQSGRQQPMHRKSVHQARADYEAAAPMLDLPPDDLAERRALEI Sbjct: 1 MPLHPDLDAFLALVEAGIQSGRQQPMHRKSVHQARADYEAAAPMLDLPPDDLAERRALEI 60 Query: 61 PTRDGARIGARLYAPAPLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLA 120 PTRDGARIGARLYAPAPLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLA Sbjct: 61 PTRDGARIGARLYAPAPLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLA 120 Query: 121 VDYRLAPEHRFPTAFEDAADAYDWLLQHGAALGLATNRLSVGGDSAGGTLAAALALRARQ 180 VDYRLAPEHRFPTAFEDAADAYDWLLQHG ALGLATNRL+VGGDSAGGTLAAALALRARQ Sbjct: 121 VDYRLAPEHRFPTAFEDAADAYDWLLQHGPALGLATNRLAVGGDSAGGTLAAALALRARQ 180 Query: 181 ADRAPCLQVLLYPCTSAHQDGDSHRRFATGYLLEGETLQWMFGHALNGDHERQDWRFAPL 240 A RAPCLQVLLYPCTSAHQDGDSHRRFATGYLLEGETLQWMFGH LNGDHERQDWRFAPL Sbjct: 181 AARAPCLQVLLYPCTSAHQDGDSHRRFATGYLLEGETLQWMFGHTLNGDHERQDWRFAPL 240 Query: 241 QAADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLRLGMVVAE 300 QAADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLRLGMVVAE Sbjct: 241 QAADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLRLGMVVAE 300 Query: 301 ADQARTAIAARLAAAMR 317 ADQARTAIAARLAAAMR Sbjct: 301 ADQARTAIAARLAAAMR 317 >gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 151 bits (381), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 103/312 (33%), Positives = 157/312 (50%), Gaps = 17/312 (5%) Query: 3 LHPDLDAFLALVEAGIQSGRQQPMHRKSVHQARADYEAA--APMLDLPPDDLAERRALEI 60 + P AFL +EAG +P+ + S ARA A + L LP D++E+ Sbjct: 28 VEPTTQAFLQALEAG----GGKPLEQLSPADARAVLVGAQASVKLQLPKADVSEKTI--- 80 Query: 61 PTRDGARIGARLYAPAPLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLA 120 + DG +I + PA A P ++FHGGG+ +G +H+ L L A + + Sbjct: 81 -SADGQQIKLTIVRPAG-SAKKTLPAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF 138 Query: 121 VDYRLAPEHRFPTAFEDAADAYDWLLQHGAALGLATNRLSVGGDSAGGTLAAALALRARQ 180 V+Y +PE + A A A W+ +HG + + RL+V G+S GG +AA +AL A+ Sbjct: 139 VNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAKD 198 Query: 181 ADRAPCL--QVLLYPCTSAHQDGDSHRRFATGYLLEGETLQWMFGHALNGDHERQDWRFA 238 P L +VLL+P T A+ + S+ +FA GY L ++W + +R++ + Sbjct: 199 KG-GPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITAS 257 Query: 239 PLQA--ADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLRLGM 296 PL A A +SG P L+ + D L DEG Y KL AGV V + GM+HDF L Sbjct: 258 PLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAVTSVRYNGMIHDFGLLN- 316 Query: 297 VVAEADQARTAI 308 V+A+ +TA+ Sbjct: 317 VLAKVPAVQTAM 328 >gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 97.8 bits (242), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 86/231 (37%), Positives = 114/231 (49%), Gaps = 7/231 (3%) Query: 67 RIGARLYAPA-PLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLAVDYRL 125 RI RLY PA PL +P+++YFHGGG+ GSL+ DT +A VL+V Y L Sbjct: 65 RIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHGQLAVLSVGYAL 124 Query: 126 APEHRFPTAFEDAADAYDWLLQHGAALGLATNRLSVGGDSAGGTLAAALALRARQADRAP 185 AP FP A EDA A W ++ L + +R+ V GD AGG LAA+L L R P Sbjct: 125 APAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGKP 184 Query: 186 -CLQVLLYPCT--SAHQDGDSHRRFATGYLLEGETLQWMFGHALNGDHERQDWRFAPLQA 242 QVL+ P S GD+ R + L ET + L +R APL+ Sbjct: 185 LAAQVLISPMLDPSMRLLGDAARLQSD---LTAETCAASYRQYLPQTMQRLHPYAAPLEV 241 Query: 243 ADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDFLR 293 + ++G PA + + D L E YA L +AGVP Q+ G+ H LR Sbjct: 242 SRLAGLPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFAGITHAALR 292 >gi|300311008|ref|YP_003775100.1| esterase/lipase/thioesterase family protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 72.4 bits (176), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 51/123 (41%), Positives = 67/123 (54%), Gaps = 3/123 (2%) Query: 64 DGARIGARLYAPAPLDAAAPQ--PVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLAV 121 D AR +Y PA +A PQ PV+++F+GG + GS + + ALA+R VL Sbjct: 51 DNARQQLDIYRPADTASAPPQGRPVVVFFYGGSWNQGSRKDYAFVGEALASRGVIAVLP- 109 Query: 122 DYRLAPEHRFPTAFEDAADAYDWLLQHGAALGLATNRLSVGGDSAGGTLAAALALRARQA 181 DYRL PE +P +D+A A W L+ ALG R+ V G SAG AA +AL AR Sbjct: 110 DYRLYPEVSYPDFLQDSAQAVAWTLRSLQALGGDPQRVFVMGHSAGAYNAAMMALDARWL 169 Query: 182 DRA 184 D A Sbjct: 170 DAA 172 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 984,504 Number of extensions: 40569 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 4 Length of query: 317 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 232 Effective length of database: 1,217,395 Effective search space: 282435640 Effective search space used: 282435640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)