BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0019.0002.p (244 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311663|ref|YP_003775755.1| iron reductase [Herbaspirillum ... 471 e-134 >gi|300311663|ref|YP_003775755.1| iron reductase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 471 bits (1213), Expect = e-134, Method: Compositional matrix adjust. Identities = 236/244 (96%), Positives = 238/244 (97%) Query: 1 VLPILAPLFDQRTAAYAARLVCDASSYAQALPLPALLEASALDDAVRRHAAHWRSDDLRA 60 VLPILAPLFDQRTAAYAARLVCD SSYAQALPLPALLE +ALDDAVR HAAHWRSDDLRA Sbjct: 27 VLPILAPLFDQRTAAYAARLVCDVSSYAQALPLPALLEGAALDDAVRLHAAHWRSDDLRA 86 Query: 61 AASAWCLDYFAAMLVPAMAAAAVLRHHLPLAGVQLRVVLAPHGGMAQVCLPHAGHPWPQA 120 ASAWCLDYFAAMLVPAMAAAAVLRHHLPLA VQ+RVVLA HGGMAQVCLPHAGHPWPQA Sbjct: 87 VASAWCLDYFAAMLVPAMAAAAVLRHHLPLAAVQVRVVLASHGGMAQVCLPHAGHPWPQA 146 Query: 121 SPPERYEELVWQHLAPVIAALNKRFRTPSAVLWGNAVRYIHAVLQRGVLLALPSQEGLAE 180 SPPERYEELVWQHLAPVIAALNKRFRTPSAVLWGNAVRYIHAVLQRGVLLALPSQEGLAE Sbjct: 147 SPPERYEELVWQHLAPVIAALNKRFRTPSAVLWGNAVRYIHAVLQRGVLLALPSQEGLAE 206 Query: 181 DRRLLLDSPLWPDGRTNPLYHRRAVPPAGVDYLHRNCCMYYRLPGEDYCAACPLRSRHDK 240 DRRLLLDSPLWPDGRTNPLYHRRAVPPAGVDYLHRNCCMYYRLPGEDYCAACPLRSRHDK Sbjct: 207 DRRLLLDSPLWPDGRTNPLYHRRAVPPAGVDYLHRNCCMYYRLPGEDYCAACPLRSRHDK 266 Query: 241 RASD 244 RASD Sbjct: 267 RASD 270 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.136 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 778,854 Number of extensions: 32461 Number of successful extensions: 114 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 114 Number of HSP's successfully gapped: 1 Length of query: 244 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 161 Effective length of database: 1,226,865 Effective search space: 197525265 Effective search space used: 197525265 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 129 (54.3 bits)