BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0114.p (551 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion ... 1115 0.0 gi|300310625|ref|YP_003774717.1| hemolysin activation translocat... 371 e-104 >gi|300312549|ref|YP_003776641.1| hemolysin activation/secretion protein [Herbaspirillum seropedicae SmR1] Length = 584 Score = 1115 bits (2885), Expect = 0.0, Method: Compositional matrix adjust. Identities = 547/551 (99%), Positives = 550/551 (99%) Query: 1 MSSAWAQADLSTQQFQRQQERERELRRQQERGPDVRLPAQPGAAAEAVPAQESPCFAVHR 60 MSSAWAQADLSTQQFQRQQERERELRRQQERGPDVRLPAQPGAAAEAVPAQESPCFAVHR Sbjct: 34 MSSAWAQADLSTQQFQRQQERERELRRQQERGPDVRLPAQPGAAAEAVPAQESPCFAVHR 93 Query: 61 VVLDGAGSARFDWLLDLPEANPGGRCLGVQGINAVMSALQNAIVARGYVTTRLLAQPQDI 120 VVLDG GSARFDWLLDLPEANPGGRCLGVQGINAVMSALQNAIVARGYVTTR+LAQPQDI Sbjct: 94 VVLDGPGSARFDWLLDLPEANPGGRCLGVQGINAVMSALQNAIVARGYVTTRILAQPQDI 153 Query: 121 ASGELRLTIVPGKIRAIRFADNADPRATQWNALPASPGDLLNLRDIEQGLENLKRVPTAE 180 ASGELRLTIVPGKIRAIRFADNADPRATQWNALPASPGDLLNLRDIEQGLENLKRVPTAE Sbjct: 154 ASGELRLTIVPGKIRAIRFADNADPRATQWNALPASPGDLLNLRDIEQGLENLKRVPTAE 213 Query: 181 ADIQIAPSEGKDAAPGDSDLVISYQQGIPIRLTLSVDDSGTKATGKYQGSATVSLDNWLT 240 AD+QIAPSEGKDAAPGDSDLVISYQQGIPIRLTLSVDDSGTKATGKYQGSATVSLDNWLT Sbjct: 214 ADVQIAPSEGKDAAPGDSDLVISYQQGIPIRLTLSVDDSGTKATGKYQGSATVSLDNWLT 273 Query: 241 LNDLFYISFSHDLGGGDPGARGSQSQVVHYSLPLGYWALAFTNSSNRYHQAVAGINQTYL 300 LNDLFYISFSHDLGGGDPGARGSQSQVVHYSLPLGYWALAFTNSSNRYHQAVAGINQTYL Sbjct: 274 LNDLFYISFSHDLGGGDPGARGSQSQVVHYSLPLGYWALAFTNSSNRYHQAVAGINQTYL 333 Query: 301 YSGQSETNELKLSRLVYRDAVRKTTLSFKGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAH 360 YSGQSETNELKLSRLVYRDAVRKTTLSFKGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAH Sbjct: 334 YSGQSETNELKLSRLVYRDAVRKTTLSFKGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAH 393 Query: 361 REFIGEGTLDLALAYKRGTGAFDAMAAPEEAFGEGTSRFRLMTADLNLNAPFALPMPWGK 420 REFIGEGTLDLALAYKRGTGAFDAMAAPEEAFGEGTSRFRLMTADLNLNAPFALPMPWGK Sbjct: 394 REFIGEGTLDLALAYKRGTGAFDAMAAPEEAFGEGTSRFRLMTADLNLNAPFALPMPWGK 453 Query: 421 QAMRYTLGARAQWNRTPLTPQDKFSLGGRYSVRGFDGESNLLAERGWYVRNELAAALGAS 480 QAMRYTLGARAQWNRTPLTPQDKFSLGGRYSVRGFDGESNLLAERGWYVRNELAAALGAS Sbjct: 454 QAMRYTLGARAQWNRTPLTPQDKFSLGGRYSVRGFDGESNLLAERGWYVRNELAAALGAS 513 Query: 481 KQEIYAGLDYGEVGGPSTELLVGRRLAGAVLGLRGALGRTNYEVFAGVPISKPDRFKTSP 540 KQEIYAGLDYGEVGGPSTELLVGRRLAGAVLGLRGALGRTNYEVFAGVPIS+PDRFKTSP Sbjct: 514 KQEIYAGLDYGEVGGPSTELLVGRRLAGAVLGLRGALGRTNYEVFAGVPISQPDRFKTSP 573 Query: 541 LTAGMSLMWSF 551 LTAGMSLMWSF Sbjct: 574 LTAGMSLMWSF 584 >gi|300310625|ref|YP_003774717.1| hemolysin activation translocator protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 371 bits (953), Expect = e-104, Method: Compositional matrix adjust. Identities = 219/539 (40%), Positives = 313/539 (58%), Gaps = 33/539 (6%) Query: 33 PDVRLPAQPGAA--AEAVPAQESPCFAVHRVVL------------DGAGSARFD--WLLD 76 P V LP Q A ES CF V RV L G + + D + Sbjct: 56 PSVSLPRQRSDEDLQSYTLAPESLCFPVQRVTLAFPEQVSEAVRRTGGFALQMDPFHFIQ 115 Query: 77 LPEANPGGRCLGVQGINAVMSALQNAIVARGYVTTRLLAQPQDIASGELRLTIVPGKIRA 136 A G CLG QGI ++ + I+ RGY TTR+ QD+++G+L +T+VPG I A Sbjct: 116 TYLAGYAGSCLGQQGIETILRRVTGQILERGYSTTRVGIGQQDLSTGQLSITLVPGIIHA 175 Query: 137 IRFADNADPRATQWNALPASPGDLLNLRDIEQGLENLKRVPTAEADIQIAPSEGKDAAPG 196 IRF D A A+ +A P G LLNLRD+EQGLE +KRV + + D+QI P++ PG Sbjct: 176 IRFKDPA-ITASYRSAFPTQAGALLNLRDLEQGLEQMKRVASQDVDMQILPTD----VPG 230 Query: 197 DSDLVISYQQGIPIRLTLSVDDSGTKATGKYQGSATVSLDNWLTLNDLFYISFSHD---L 253 SD+V+ ++ P +LTLS+DDSG K+TGKYQ +++D+ L +NDLF FS D + Sbjct: 231 QSDVVLEVKRIKPWKLTLSLDDSGAKSTGKYQAGINLAIDDLLGINDLFNAGFSSDADRM 290 Query: 254 GGGDPGARGSQSQVVHYSLPLGYWALAFTNSSNRYHQAVAGINQTYLYSGQSETNELKLS 313 G ARG+ YS P GYW + S++RYHQ +AG+ QT++ SG SE E+++ Sbjct: 291 GQ----ARGTTGNNFSYSAPAGYWTFGVSGSTSRYHQRIAGLFQTFVSSGHSENLEVRVQ 346 Query: 314 RLVYRDAVRKTTLSFKGFVRSSSNYVDDTEVQVQRRRVAGWEMGLAHREFIGEGTLDLAL 373 +L RDA +K +L + R S +Y+DDTE++VQRR E+G HR + G+ LD L Sbjct: 347 KLFQRDAQQKNSLQLRIGKRWSRSYIDDTEIEVQRRNTTFAELGWMHRHYFGDVQLDATL 406 Query: 374 AYKRGTGAFDAMAAPEEAFGEG-TSRFRLMTADLNLNAPFALPMPWGKQAMRYTLGARAQ 432 A + G F+ A + +G T R+ + T D + APF L G Q++ Y R Q Sbjct: 407 ANRWGVSWFNGAADAPDRLSDGPTYRYSMQTIDATVVAPFKL----GGQSLTYVGTLRGQ 462 Query: 433 WNRTPLTPQDKFSLGGRYSVRGFDGESNLLAERGWYVRNELAAALGASKQEIYAGLDYGE 492 +R+ L D+F++G RY+VRGFDGE L +E G ++RN+L ALG++ +YAGLD G Sbjct: 463 HSRSRLYLTDQFAIGSRYTVRGFDGELTLASESGVFLRNDLEIALGSTGAALYAGLDAGR 522 Query: 493 VGGPSTELLVGRRLAGAVLGLRGALGRTNYEVFAGVPISKPDRFKTSPLTAGMSLMWSF 551 + GPS L G LAGA +GLRGA+ +YE+FAG + +P++F+T+ AG +L++ F Sbjct: 523 LYGPSVANLQGDTLAGAAIGLRGAVSGLHYELFAGWALVRPEQFQTAMPAAGFNLIYQF 581 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,697,233 Number of extensions: 73356 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 3 Length of query: 551 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 461 Effective length of database: 1,193,720 Effective search space: 550304920 Effective search space used: 550304920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)