BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0095.p (516 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 984 0.0 gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 272 4e-74 gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 249 5e-67 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 181 2e-46 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 177 2e-45 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 984 bits (2543), Expect = 0.0, Method: Compositional matrix adjust. Identities = 510/516 (98%), Positives = 513/516 (99%), Gaps = 1/516 (0%) Query: 1 MNNNSSTLDLPIQPPQRSGRAWPRAVSVVAAVLAATLMAGCASFTDLGERAQPKPIDRYQ 60 MN+NSSTLDLPIQPPQRSGRAWPRAVSV+AAVLAATLMAGCASF+DLGERAQPK IDRYQ Sbjct: 1 MNSNSSTLDLPIQPPQRSGRAWPRAVSVLAAVLAATLMAGCASFSDLGERAQPKSIDRYQ 60 Query: 61 SQQSLAASAVQAAWPSDQWWRVYGDAQLNALIDEALQSAPSMAVAKARLMKAEGAAQQQG 120 SQQSLAASAVQAAWPSDQWWRVYGDAQLNALIDEALQSAPSMAVAKARLMKAEGAAQQQG Sbjct: 61 SQQSLAASAVQAAWPSDQWWRVYGDAQLNALIDEALQSAPSMAVAKARLMKAEGAAQQQG 120 Query: 121 AALYPQVSANASLDRMKQSYNNGVPPDFVPKDYNNATRATLDFSYEIDFWGKNRAALAAA 180 AALYPQVSANASLDRMKQSYNNGVPPDFVPKDYNNATRATLDFSYEIDFWGKNRAALAAA Sbjct: 121 AALYPQVSANASLDRMKQSYNNGVPPDFVPKDYNNATRATLDFSYEIDFWGKNRAALAAA 180 Query: 181 TSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNG 240 TSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNG Sbjct: 181 TSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNG 240 Query: 241 LETRGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPF 300 LETRGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPF Sbjct: 241 LETRGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPF 300 Query: 301 ALPTQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLTR 360 ALP QLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLTR Sbjct: 301 ALPAQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLTR 360 Query: 361 AGSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKA 420 AGSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKA Sbjct: 361 AGSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKA 420 Query: 421 LAGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTL 480 LAGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTL Sbjct: 421 LAGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTL 480 Query: 481 DVALVRALGGGYRAADDSSSSQNSADAPLHDTQAKG 516 DVALVRALGGGYRAADD SSSQNSADAPLHDTQAKG Sbjct: 481 DVALVRALGGGYRAADD-SSSQNSADAPLHDTQAKG 515 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 272 bits (696), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 181/463 (39%), Positives = 261/463 (56%), Gaps = 6/463 (1%) Query: 39 AGCASFTDLGERAQPKPIDRYQSQQSLA---ASAVQAAWPSDQWWRVYGDAQLNALIDEA 95 +GCASF +G Q + +Q+SL+ A WP W R +GDAQL AL+++A Sbjct: 27 SGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGAPNGQWPGSDWVRQFGDAQLVALVEQA 86 Query: 96 LQSAPSMAVAKARLMKAEGAAQQQGAALYPQVSANASLDRMKQSYNNGVPPDFVPKDYNN 155 L S+PS+ A+AR+ A A+ +GA L P V+A AS+ R + S PP + YN Sbjct: 87 LTSSPSLQQARARIAAASALAESRGAPLLPSVNAEASVTRNQFSSTTIYPPPYGGNWYNE 146 Query: 156 ATRATLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAYAELAQLYAQ 215 +A L+ YE+D W KN+AALA A S +A++A +AR+ L SI + Y++LA YA Sbjct: 147 -KKAGLNVGYELDLWNKNQAALAQAISSEKAAQASEQEARLALTASIVTVYSQLAAQYAL 205 Query: 216 RDTNEAALKVRVESLDLFNQRFTNGLETRGSVKQMEARRAIAQADLKATDESIGLQRNKL 275 D ++ + R + +R GL+++ Q A A+A L ++ I L R +L Sbjct: 206 HDILQSTVDQRTSLEKITAERLRTGLDSQIERDQSRTSSADARAQLAQSEGQIVLLRQQL 265 Query: 276 AALLGAGPDRGLQLTRPQID-LSRPFALPTQLPVELLGRRPDIVAARLRAEAAGKQIKVA 334 AL G GPD GLQL P + L+ P LP +LP+ L+GRRPDIVAAR + EAA + + VA Sbjct: 266 GALAGKGPDYGLQLAPPALQGLATP-GLPAELPLNLMGRRPDIVAARWQVEAASRGVDVA 324 Query: 335 RAAFYPNVNLTAYFGFQSLGIDMLTRAGSDIGSIGPAISLPIFNGGRLRGQFRSASASYD 394 +A FYP++NL+A GF +L A S + GPAI+LPIF GG LR + ASY+ Sbjct: 325 KARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYASYE 384 Query: 395 EAVANYDQAVTQALQDVADVGVSEKALAGRLADVQAAADAAEEAYRIVSNRYNGGLATYL 454 AVA Y++ + A DVA + A +L A AAE AY + RY GL + L Sbjct: 385 LAVATYNKTLNDAYADVARQIAAIHATERQLPIRSEALQAAERAYALARERYRLGLVSQL 444 Query: 455 DVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGGYRAADD 497 +L+AQ +++ + + L+++ VAL +ALGGG+ A D Sbjct: 445 TLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGGGFDAQRD 487 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 249 bits (635), Expect = 5e-67, Method: Compositional matrix adjust. Identities = 186/471 (39%), Positives = 264/471 (56%), Gaps = 6/471 (1%) Query: 29 VAAVLAATL-MAGCASFTDLGERAQPKPIDRYQSQQSLAASAVQAAWPSDQWWRVYGDAQ 87 V V+A +L MAGCA + +AQ + + + Q++ +A +A WP WW+ YGDAQ Sbjct: 11 VLTVIALSLTMAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQRAWWQAYGDAQ 70 Query: 88 LNALIDEALQSAPSMAVAKARLMKAEGAAQQQGAALYPQVSANASLDRMKQSYNNGVPPD 147 L+ L+ A+ PSMAVA AR+ +A+ A A P + S+ R S N Sbjct: 71 LDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDWSDNVYYGAA 130 Query: 148 FVPK-DYNNATRATLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAY 206 F K +NN ATL SY +D W +N+ A A E+ A ADA A++ L ++ Y Sbjct: 131 FRNKLTWNNT--ATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDLEGNVVRTY 188 Query: 207 AELAQLYAQRDTNEAALKVRVESLDLFNQRFTNGLETRGSVKQMEARRAIAQADLKATDE 266 +LA Y D EA LK + LDL ++RF GL T+ + Q E+ + ++A E Sbjct: 189 VQLALQYGLLDNTEALLKEEQKILDLAHRRFKGGLGTQLEITQAESPLPETRRQIEALHE 248 Query: 267 SIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPFALPTQLPVELLGRRPDIVAARLRAEA 326 +I L RN+LAALLGAGP +TRP + L LP+ LP +L+GRRPDI AAR R EA Sbjct: 249 NIALIRNQLAALLGAGPGAADGVTRPGMQLQTSLGLPSALPADLVGRRPDISAARWRVEA 308 Query: 327 AGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLT--RAGSDIGSIGPAISLPIFNGGRLRG 384 A K I VA+AAFYPN+NL A G + G + + A + + GPAISLPIF GGRLRG Sbjct: 309 AAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPIFEGGRLRG 368 Query: 385 QFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGRLADVQAAADAAEEAYRIVSN 444 Q +ASA YD V +Y+ ++QAL+ ++D V+ +++A + Q + AA++ I + Sbjct: 369 QLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQKNVDIATR 428 Query: 445 RYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGGYRAA 495 Y GL YL+VL+AQ + + + L+++ AL ALGGG A Sbjct: 429 AYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEA 479 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 181 bits (458), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 158/471 (33%), Positives = 236/471 (50%), Gaps = 33/471 (7%) Query: 40 GCASFTDLGERAQPKPIDRYQSQQSLAASAVQAAWPSDQWWRVYGDAQLNALIDEALQSA 99 GCAS D R Q + S+ + A P WW +GDAQLNAL +AL Sbjct: 18 GCASGPDY-TRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAGN 76 Query: 100 PSMAVAKARLMKAEGAAQQQGAALYPQVSANASLDRMKQSYNNGVPPDFVPKDYNNATRA 159 ++A+A ARL +A GAA PQV+ NA R + S N + +YN+ + Sbjct: 77 QTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPL------TNYNSPNFS 130 Query: 160 TL--DF------SYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAYAELAQ 211 T+ DF SYE+DF+G+ + ++ AA + E S AD R+ L +A+ Y L + Sbjct: 131 TVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRE 190 Query: 212 LYAQRDTNEAALKVRVESLDLFNQRF----TNGLETRGSVKQMEARRAIAQADLKATDES 267 L + D A+ ++ ++L+L R T+GL+ M ++A+ + L D Sbjct: 191 LDVELDVVRRAIALQRQALELATARHDLGATSGLD-------MAQQQALLDSTLTQID-L 242 Query: 268 IGLQR----NKLAALLGAGPDRGLQLTRPQIDLSRPFALPTQLPVELLGRRPDIVAARLR 323 +G QR + +A L G P +T P + P A+P +P ++L RRPDI AA Sbjct: 243 LGRQRAQYEHAIATLTGT-PAPAFSIT-PSLTPITPPAIPVGVPSDVLERRPDIAAAERA 300 Query: 324 AEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLTRAGSDIGSIGPAISLPIFNGGRLR 383 AA QI VA AA+YP+ L +G S L A S + S+G + S +F+GGRLR Sbjct: 301 MAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLR 360 Query: 384 GQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGRLADVQAAADAAEEAYRIVS 443 + A Y+ VA+Y + V A+Q+V D AL A Q A +A I S Sbjct: 361 AGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIAS 420 Query: 444 NRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGGYRA 494 +RY GG YLDV+ AQ +L+++ RQ + L + + V L++ALGG +++ Sbjct: 421 SRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQS 471 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 177 bits (449), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 145/431 (33%), Positives = 215/431 (49%), Gaps = 25/431 (5%) Query: 71 QAAWPSDQWWRVYGDAQLNALIDEALQSAPSMAVAKARLMKAEGAAQQQGAALYPQVSAN 130 +AA P W + D L ALI +AL+++ + A R+ +A+ A L+P + A Sbjct: 58 EAASPPLPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQ 117 Query: 131 ASLDRMKQSYNNGVPPDFVPKDYN--------NATRATLDFS-YEIDFWGKNRAALAAAT 181 A LDR + P D N +A +A L S +EID WG+ R+ AA Sbjct: 118 AGLDRSR-----------TPADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAAL 166 Query: 182 SELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNGL 241 AS+A + ++L +A A+ ELA+L + + + R ES +F++R G Sbjct: 167 ESARASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGA 226 Query: 242 ETRGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDLSRPFA 301 +R ++ Q+E AQA + + N L L+G+ L + + + L FA Sbjct: 227 TSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGS--PVVLPVGQARAGLDAVFA 284 Query: 302 LPTQ--LPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLT 359 P Q LP LL RRPD+ AA R AA I ARAAF+P+++LT G S + L Sbjct: 285 -PLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLF 343 Query: 360 RAGSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEK 419 R GS + P+I+LPIF GRLR + D AVA Y++ V A ++VAD + + Sbjct: 344 RDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQ 403 Query: 420 ALAGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFT 479 L +L Q+A E R+ + RY+ G A +LDVL+AQ L+S +QL +R + + Sbjct: 404 WLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLS 463 Query: 480 LDVALVRALGG 490 VAL ALGG Sbjct: 464 SSVALYAALGG 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,255,267 Number of extensions: 45045 Number of successful extensions: 299 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 237 Number of HSP's successfully gapped: 20 Length of query: 516 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 427 Effective length of database: 1,198,455 Effective search space: 511740285 Effective search space used: 511740285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)