BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0094.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 791 0.0 gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 247 1e-66 gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 132 6e-32 gi|300311270|ref|YP_003775362.1| HlyD family membrane-fusion pro... 59 7e-10 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 57 2e-09 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 791 bits (2044), Expect = 0.0, Method: Compositional matrix adjust. Identities = 409/411 (99%), Positives = 409/411 (99%), Gaps = 1/411 (0%) Query: 4 MSHSDSQAAHTLAEEAKQRNEAASAAAAAQKRKKLFSIFGGVVAIAAIGYGAYWYLIGSR 63 MSHSDSQAAHTLAEEAKQRNEAASAAAAAQKRKKLFSIFGGVVAIAAIGYGAYWYLIGSR Sbjct: 1 MSHSDSQAAHTLAEEAKQRNEAASAAAAAQKRKKLFSIFGGVVAIAAIGYGAYWYLIGSR 60 Query: 64 YVETDNAYTATEIATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADARLAVDQAAADL 123 YVETDNAYTATEIATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADARLAVDQAAADL Sbjct: 61 YVETDNAYTATEIATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADARLAVDQAAADL 120 Query: 124 DRTERKVKGFFANDAGLAAQVLAREAEQKRASAQLLSAQADLKRAEIDLQRREALAKSGS 183 DRTERKVKGFFANDAGLAAQVLAREAEQKRASAQLLSAQADLKRAEIDLQRREALAKSGS Sbjct: 121 DRTERKVKGFFANDAGLAAQVLAREAEQKRASAQLLSAQADLKRAEIDLQRREALAKSGS 180 Query: 184 VSGEELSNARTALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVLTANTTVDDNPEVV 243 VSGEELSNARTALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVLTANTTVDDNPEVV Sbjct: 181 VSGEELSNARTALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVLTANTTVDDNPEVV 240 Query: 244 LAR-KLEQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKE 302 LAR KLEQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKE Sbjct: 241 LARAKLEQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKE 300 Query: 303 GQLTKVRIGQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLP 362 GQLTKVRIGQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLP Sbjct: 301 GQLTKVRIGQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLP 360 Query: 363 VRISLDPKELAQRPLSVGLSMVVEIDTRGQIQAGDAQPKSARNDNAQAAAL 413 VRISLDPKELAQRPLSVGLSMVVEIDTRGQIQAGDAQ KSARNDNAQAAAL Sbjct: 361 VRISLDPKELAQRPLSVGLSMVVEIDTRGQIQAGDAQRKSARNDNAQAAAL 411 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 247 bits (631), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 159/370 (42%), Positives = 223/370 (60%), Gaps = 29/370 (7%) Query: 34 KRKKLFSIFGGVVAIAAIGYGAYWYLIGSRYVETDNAYTATEIATVTPAINGIVAAVDVV 93 KRK+ + V+ + A+ YW+L + ETD+AY + ++ + G V AV Sbjct: 19 KRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVKAD 78 Query: 94 DTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGFFANDAGLAAQVLAREAEQKR 153 DTQ VK G LV +D AD RLA+DQA A L + R+ + F N+ LAA V A Sbjct: 79 DTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFLNNDTLAANVAA------- 131 Query: 154 ASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQS 213 A ++L RA DLQRR+A SG+VS E++S+AR A+ +A A L A AA Sbjct: 132 -------ADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQARAAA--- 181 Query: 214 RANIKATQGAQKASTVLTANTTVDDNPEVV-LARKLEQAKLDLERTVLRAPVDGVIARRQ 272 A+ LT +T+V ++P V+ A + A L+ R + APV G +++R Sbjct: 182 -----------AANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRS 230 Query: 273 VQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKADLYGGSVEYHGVVT 332 VQVGQR+ +G L+++VPL+Q+ +DANFKE QL +RIGQPV + AD+YG SV+Y G V Sbjct: 231 VQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVI 290 Query: 333 GLSGGTGSAFAVIPAQNATGNWIKVVQRLPVRISLDPKELAQRPLSVGLSMVVEIDTRGQ 392 G S GTG AF+++PAQNATGNWIKVVQR+PVRI+LDP+++ PL +GLS +D G Sbjct: 291 GFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGD 350 Query: 393 IQAGDAQPKS 402 +A +A P + Sbjct: 351 GRALEAVPTN 360 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 132 bits (331), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 67/143 (46%), Positives = 96/143 (67%), Gaps = 2/143 (1%) Query: 247 KLEQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLT 306 + + A ++L T +RA VDG I +QV+VGQ V G +++VVP + +V ANFKE Q+ Sbjct: 232 QYDAASVNLGYTEIRASVDGRIGDKQVRVGQFVSPGTRMMTVVPTR-FYVSANFKETQIG 290 Query: 307 KVRIGQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLPVRIS 366 +RIGQP +K D G VE+HG V LS GTG+ F+++P QNATGN+ K+VQR+PVR++ Sbjct: 291 IMRIGQPAIIKVDALPG-VEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRVPVRVA 349 Query: 367 LDPKELAQRPLSVGLSMVVEIDT 389 +D R GLS+ VE++T Sbjct: 350 IDATPEETRAFVAGLSVTVEVNT 372 >gi|300311270|ref|YP_003775362.1| HlyD family membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 358 Score = 58.9 bits (141), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 80/295 (27%), Positives = 136/295 (46%), Gaps = 44/295 (14%) Query: 41 IFGGV--VAIAAIGY-GAYWYL---IGSRYVETDNAYTATEIATVTPAINGIVAAVDVVD 94 +F G+ V +AA+G+ G W+ + V ++ ATE+ + + G + + V + Sbjct: 9 LFAGLLLVVLAAVGWTGWAWWRDADAPAGLVSSNGRLEATEV-DIGSRLGGRIRHMLVDE 67 Query: 95 TQAVKKGDVL--VRIDDADARLAVDQAAADLDRTERKVKGFFANDAGLAAQVLAREAEQK 152 +AV+ G VL +++D A+L +A + +R +G A LA R +EQ+ Sbjct: 68 GEAVQAGQVLAYLQVDSLHAQLREARA-----QQQRASQGVTVAQAQLAM----RRSEQR 118 Query: 153 RASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQ 212 A A + L A ++R + L + +++ +E+ + L A+A L AA+A E Sbjct: 119 IAQALTVQRHKSLSAATRRVERSQPLGRMQALTAQEVDDEENTLAEARAQLDAAQAQE-- 176 Query: 213 SRANIKATQGAQKASTVLTANTTVDDNPEVVLARKLEQA--------KLDLERTVLRAPV 264 +GA+ A A+ V AR L +A + DLE + +RAP Sbjct: 177 --------EGARAAIRAAEAD--------VASARALLEAAQAATARIEADLEESAIRAPR 220 Query: 265 DGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKAD 319 DG I R Q G+ V G+ LL+++ L +H+ KVR+GQ V + D Sbjct: 221 DGHIQYRLAQAGEVVSEGSKLLNLIDLSDLHMHFFLPAELAGKVRLGQEVRVVLD 275 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 50/162 (30%), Positives = 81/162 (50%), Gaps = 14/162 (8%) Query: 249 EQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKV 308 + A+L+LERT + +PVDG I V+ G +GA ++V+ V A F+E +L + Sbjct: 172 DTARLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYAYFEETKLPLL 231 Query: 309 RIGQPVTMKADLYGGSVEYH--GVVTGLSGGTGSAFAVIPAQ-NATGNWIKVVQRLPVRI 365 IG ++ + G S+E H + G+ + A N T NW+++ QR+PVRI Sbjct: 232 SIGDRARIQL-INGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRI 290 Query: 366 SLD--PKELAQRPLSVGLSMVVEIDTRGQIQAGDAQPKSARN 405 LD P+ + L+ GL+ V ++ QP A+N Sbjct: 291 QLDKVPENVL---LASGLTCTVVLEP-----GSGKQPAVAKN 324 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.127 0.335 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 821,777 Number of extensions: 23924 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 6 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)