BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0093.p (525 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 1048 0.0 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 417 e-117 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 206 4e-54 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 124 2e-29 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 122 5e-29 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 1048 bits (2711), Expect = 0.0, Method: Compositional matrix adjust. Identities = 524/525 (99%), Positives = 525/525 (100%) Query: 1 MSSTLSADAAGAPAPAPQPAPFEAPLSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIA 60 MSSTLSADAAGAPAPAPQPAPFEAPLSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIA Sbjct: 1 MSSTLSADAAGAPAPAPQPAPFEAPLSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIA 60 Query: 61 GALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFSALCGMANS 120 GALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFSALCGMANS Sbjct: 61 GALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFSALCGMANS 120 Query: 121 LGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVL 180 LGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVL Sbjct: 121 LGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVL 180 Query: 181 CDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLD 240 CDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLD Sbjct: 181 CDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLD 240 Query: 241 EGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTASVTTICLAF 300 EGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTASVTTICLAF Sbjct: 241 EGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTASVTTICLAF 300 Query: 301 GAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMVAKMSTTIDARKLVCIGV 360 GAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMVAKMSTTIDARKLVCIGV Sbjct: 301 GAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMVAKMSTTIDARKLVCIGV 360 Query: 361 TWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLM 420 TWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLM Sbjct: 361 TWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLM 420 Query: 421 SFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRGGDAINTMMASGMSAEQATASLAQ 480 SFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRGGDAI+TMMASGMSAEQATASLAQ Sbjct: 421 SFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRGGDAIHTMMASGMSAEQATASLAQ 480 Query: 481 TVQNQAVMLSTNHIFMMAAVSFSLAALAVWLAPKPTRVADTSAAH 525 TVQNQAVMLSTNHIFMMAAVSFSLAALAVWLAPKPTRVADTSAAH Sbjct: 481 TVQNQAVMLSTNHIFMMAAVSFSLAALAVWLAPKPTRVADTSAAH 525 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 417 bits (1071), Expect = e-117, Method: Compositional matrix adjust. Identities = 220/495 (44%), Positives = 313/495 (63%), Gaps = 8/495 (1%) Query: 13 PAPAPQPAPFEAPLSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTY 72 P+ A P P PL+G LV + ++ A F+ VLD++IANV++ TI+G LG S +GT+ Sbjct: 7 PSQAYTPPP---PLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTW 63 Query: 73 VITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQG 132 VITS+A A AI++PLTGWL R G VR+F+ S+++F L S LCG+A +L +L+A RVLQG Sbjct: 64 VITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQG 123 Query: 133 LAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWI 192 L GP++PLSQ+LLL +PK K+ A+ LW MT +VAP+AGP LGG + D Y+WP+IF+I Sbjct: 124 LVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYI 183 Query: 193 NVPVALICGYIGWRMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTE 252 N+PV LI + + + ET KLPID++GL L+V WVAA Q+MLD+GK+ DWFAS Sbjct: 184 NIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPT 243 Query: 253 IVLLAVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPL 312 IV L + A++ F F++WEL +P+VDLR+F R F I + +G FFG++V+ P Sbjct: 244 IVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQ 303 Query: 313 WLQGYMGYTATWSGCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAF 372 WLQ Y+GY + SG +A GI AV+ P++ + +DARK+ + +LR+ Sbjct: 304 WLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSR 363 Query: 373 NTTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIAT 432 + + P LQGI +FFVPLT L LS + E +AAGL SF R GA+ T Sbjct: 364 YNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGT 423 Query: 433 SIVNTSWENDATYFHAELSGLVDRGG----DAINTMMAS-GMSAEQATASLAQTVQNQAV 487 SI T+W N + HA+L+ + +N + ++ G++AEQ L +T+ QA Sbjct: 424 SISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAA 483 Query: 488 MLSTNHIFMMAAVSF 502 ML N IF ++AV F Sbjct: 484 MLGINDIFYVSAVIF 498 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 206 bits (524), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 145/493 (29%), Positives = 249/493 (50%), Gaps = 15/493 (3%) Query: 43 FIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFI 102 +A LD +I N S+ TI G +GAS ++GT++ T+Y VAE + +P++ WL G + + Sbjct: 27 LMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLRVLLL 86 Query: 103 LSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLW 162 + +F LFS LCG++ +L M+I RV QG GG L+P + T++ P+ + P ++ Sbjct: 87 TAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAMF 146 Query: 163 AMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKL-PID 221 T ++ P+ GP++GG L + SW Y F+IN+PV + + K +L D Sbjct: 147 GATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKFEQLAEAD 206 Query: 222 RVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDL 281 G+ + + + L ++L+EG+ + WF+S +I LA T+V+ FI I ++ R+P++ L Sbjct: 207 WFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHPVIQL 266 Query: 282 RVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAP 341 R+ R R F + V + A +G+ + P +L G GY + SG I L+G+ ++ P Sbjct: 267 RLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMMP 326 Query: 342 MVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPL----FLQGIGLPLFF 397 + +D R V G L AF TD+S G ++G+G L Sbjct: 327 FTPLLMRLVDIRIAVGTGFLLLA----ASAFIETDLSPLSYGGSFVASQLMRGVGTVLAM 382 Query: 398 VPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRG 457 + L A+ SV + A+GL S R + G++A + + + L ++ Sbjct: 383 LFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESLNAN 442 Query: 458 GDAI-NTMMASGM---SAEQATASLAQTVQNQAVMLSTNHIFMMAAVS-FSLAALAVWLA 512 D + ++M A G + A + T+Q+QA+ ++ +F M V+ + L ++L Sbjct: 443 SDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLVLFLR 502 Query: 513 PKPTRVADTSAAH 525 P PT A A+H Sbjct: 503 PLPTHAAPV-ASH 514 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 124 bits (311), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 111/412 (26%), Positives = 189/412 (45%), Gaps = 25/412 (6%) Query: 37 LLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFG 96 ++A+ F+ LDTTI N ++ +A +LG V+ +Y + A P +GWLA+RFG Sbjct: 57 IVASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFG 116 Query: 97 TVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAP 156 T R++ ++++F L S LC MA+SL ML+A RV+QGL G L+P+ + +LR P + Sbjct: 117 TRRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYI 176 Query: 157 AAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMS 216 AA+ ++ + P+ GP+LGG L + SW +IF IN+PV ++ +G + + ++ Sbjct: 177 AALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLG--MGAVLYFLPDDSLA 234 Query: 217 KLP-IDRVGLVLMVVWVAALQLMLD---EGKEKDWFASTEIVLLAVTAVVFFIAFLIWEL 272 P D +G VL+ + A L LD + W I L V A+ + + Sbjct: 235 GTPRFDLLGCVLLSACMVAFSLALDVPFDSHRTGW----SIALFVVAALT-VLGYWRHAS 289 Query: 273 TERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALT 332 P+ L +F F+ + + L PL LQ +GY+ +G + Sbjct: 290 VHPRPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPA 349 Query: 333 GILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIG 392 I + P +A + + + + +G A T D WPL + Sbjct: 350 AIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPD-------WPLTAAIVQ 402 Query: 393 LPLF-------FVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNT 437 L +F F + + L +++ + S L S + +A + +I T Sbjct: 403 LAIFGASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGT 454 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 122 bits (307), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 88/288 (30%), Positives = 148/288 (51%), Gaps = 6/288 (2%) Query: 37 LLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFG 96 ++AA F+ LDTTI ++ +A + G ++ + +T+Y + A+ +P++GW+A+RFG Sbjct: 35 IVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFG 94 Query: 97 TVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAP 156 + VF +++ +F L S LCG + ++ A RVLQGL G ++P+ + +++R K++ Sbjct: 95 SRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLM 154 Query: 157 AAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMS 216 A+ +VAP+ GP +GG + SW +IF +NVP L + M+ R E +S Sbjct: 155 KAIATITWPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGL-AAFTAVMMVVRNEHGVS 213 Query: 217 KLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTER- 275 K P+D VG +L AAL +L + S V L A+ + + W +R Sbjct: 214 KRPLDLVGFLLS---GAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRI 270 Query: 276 -NPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTA 322 +P++D R ++ +V T L L PL Q G TA Sbjct: 271 EHPLLDFSNLRIPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTA 318 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,503,587 Number of extensions: 61583 Number of successful extensions: 415 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 391 Number of HSP's successfully gapped: 22 Length of query: 525 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 435 Effective length of database: 1,193,720 Effective search space: 519268200 Effective search space used: 519268200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)