BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0078.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop... 611 e-176 gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 99 3e-22 gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [H... 62 7e-11 >gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 611 bits (1576), Expect = e-176, Method: Compositional matrix adjust. Identities = 297/300 (99%), Positives = 300/300 (100%) Query: 1 MRPTVAKTASRSEFLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWH 60 MRPTVAKTASRSEFLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWH Sbjct: 1 MRPTVAKTASRSEFLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWH 60 Query: 61 VIAPDWRGFGLTERSQADSYWFPDYLADLDAIIDHYAGAQAINLLGHSMGGNVVTLYAGV 120 VIAPDWRGFGLTERSQADSYWFPDYLADLDAIIDHYAG++AINLLGHSMGGNVVTLYAGV Sbjct: 61 VIAPDWRGFGLTERSQADSYWFPDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGV 120 Query: 121 RPQRIKRLINLEGLGLPATQPEQAPGRFAQWMDEVRNRPVLRGYATLEEVAQRLQRNNAR 180 RPQRIKRLINLEGLGLPATQPEQAPGRFAQWMDEVRNRPVLRGYATLEEVAQRLQRNNAR Sbjct: 121 RPQRIKRLINLEGLGLPATQPEQAPGRFAQWMDEVRNRPVLRGYATLEEVAQRLQRNNAR 180 Query: 181 LSDERAAFLAQHWAQQDDSGLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLWIEAI 240 LSDERAAFLAQHWAQQDDSGLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLWIEAI Sbjct: 181 LSDERAAFLAQHWAQQDDSGLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLWIEAI 240 Query: 241 DSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPERLAQLIEAFLQQPV 300 DSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPE+LAQLIEAFLQQPV Sbjct: 241 DSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPEQLAQLIEAFLQQPV 300 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 99.4 bits (246), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 83/298 (27%), Positives = 126/298 (42%), Gaps = 32/298 (10%) Query: 11 RSEFLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWHVIAPDWRGFG 70 R LT+ L +H+ WGDP+ + +LHG +F + L + IA D RG G Sbjct: 4 RDYLLTLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRG 63 Query: 71 LTERSQADSYWFPDYLADLDAIIDHYAGAQAINLLGHSMGGNVVTLYAGVRPQRIKRLI- 129 ++ +Y+ Y+AD++A++ Q +LLGHSMGG +YA P +++RLI Sbjct: 64 QSDWDAGANYYTDAYVADVEALVARLNLRQ-FDLLGHSMGGINAIVYAARHPAKVRRLII 122 Query: 130 ---------NLEGLGLPATQPEQAPGRFAQWMDEVRNRPVLRGYATLEEVAQRLQRNNAR 180 N G + P FA W + LR T E QRL Sbjct: 123 EDAGPGAFENSPGATRIRRELATTPSHFASWDEASTFMRALRPSVTEEARQQRLHNML-- 180 Query: 181 LSDERAAFLAQHWAQQDDSGLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLWIEAI 240 + AA W + D G+ + ++SP L R ++ + P L + Sbjct: 181 ---KPAAQGGYTW-RYDHVGI-------SSTRLSPDLSRVVDLRPHIMALMCPTLVVRGE 229 Query: 241 DSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPERLAQLIEAFLQQ 298 SD A +++ R+ IADAGH +H DQPE A+++ FL Q Sbjct: 230 RSDYLSAEMAAEMRQ--------LNARILTSTIADAGHYIHDDQPEAFAEVVRQFLAQ 279 >gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 61.6 bits (148), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 82/307 (26%), Positives = 129/307 (42%), Gaps = 36/307 (11%) Query: 1 MRPTVAKTASRSEFLTIRGLRYHVRHWGDPEAPKIFM-LHGWMDMSASFQFMVDALQGQW 59 M V KT + + Y + WGD P + + +HG +S F M L + Sbjct: 1 MTEAVVKTVQCISPAGLHTMAY--KEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTY 58 Query: 60 HVIAPDWRGFGLTER-SQADSYWFPDYLADLDAIIDHYAGAQAINLLGHSMGGNVVTLYA 118 VI PD G G + R + Y P Y++D+ ++ AQ ++ G SMGG + A Sbjct: 59 RVICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLARL-DAQIVDWFGTSMGGLIGMALA 117 Query: 119 GVRPQRIKRLINLEGLGLPATQPEQAPGRFAQWM-DEVRNRPVLRGYATLEEVAQRLQRN 177 + I+RL+ L +G P+ A R ++ +VR + + EE AQ ++ Sbjct: 118 SLPGNPIRRLV-LNDIG-PSIN-GAALARIGDYIGQDVR-------FDSFEEAAQYIRTI 167 Query: 178 NARL---SDERAAFLAQHWAQQDDSGLWQILGDPA----HKQVSPILYRADEMM--ACWR 228 +A SDE LA+ +Q++ G W D A KQ +P A E M A + Sbjct: 168 SASFGQHSDEEWHKLAKDVLRQNEQGQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYD 227 Query: 229 RITAPVLWIEAIDSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGH---MLHHDQP 285 I P L + SD+ +E+ RR P+ ++ + D GH +H Q Sbjct: 228 AIRCPTLLVRGAQSDLL-----LPEVAQEMTRR---GPKAKLVELPDVGHAPTFMHAPQI 279 Query: 286 ERLAQLI 292 E Q + Sbjct: 280 EVARQFL 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,058,190 Number of extensions: 48007 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 4 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)