BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0075.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 854 0.0 gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 211 1e-55 gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 115 6e-27 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 854 bits (2207), Expect = 0.0, Method: Compositional matrix adjust. Identities = 411/413 (99%), Positives = 412/413 (99%) Query: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR Sbjct: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 Query: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW Sbjct: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 Query: 121 MTVGAQGAKVSMVDATDVKNVEAAITPNTRLVFVETIANPRTQVADLKRIGELCRERGIL 180 MTVGAQGAKVSMVDATDVKNVEAAIT NTRLVFVETIANPRTQVADLKRIGELCRERGIL Sbjct: 121 MTVGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGIL 180 Query: 181 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEYDWTRYPHIAENY 240 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGE+DWTRYPHIAENY Sbjct: 181 YVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAENY 240 Query: 241 KKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQA 300 KKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQA Sbjct: 241 KKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQMLQA 300 Query: 301 DERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFDYLNRLRLAIPTSNLGD 360 DERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFDYLNRLRLAIPTSNLGD Sbjct: 301 DERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFDYLNRLRLAIPTSNLGD 360 Query: 361 TRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQALDA 413 TRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQALDA Sbjct: 361 TRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVADFRQALDA 413 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 211 bits (536), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 135/431 (31%), Positives = 223/431 (51%), Gaps = 21/431 (4%) Query: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 M D + F T +H ++ PI+ +V F ++D + A++F K G Y R Sbjct: 1 MPDNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 Query: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 NPT LE ++ +E G + A+G AA+ +Q + GD++VS++ L+G T +L+ Sbjct: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 Query: 121 M-TVGAQGAKVSMVDATDVKNVEAAITPNTRLVFVETIANPRTQVADLKRIGELCRERGI 179 T+ G + D + E I T+ +++E+I NP + D+ + ++ G+ Sbjct: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 Query: 180 LYVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEYDWTRYPHIAEN 239 +VDNT+ +PYL RP GA +VV+SLTK +GGHG LGGA+ D+G++ W ++ + Sbjct: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKH---KQR 237 Query: 240 YKKNPAPQWG---------------MAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQ 284 +K+ P + + R LR+ G +L P A I G ET+ LR Sbjct: 238 FKRLNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRL 297 Query: 285 ERECKNALALAQMLQADERVAAVYYPGLESHPQHALSKALFRSFGS-LMSFELKDGID-C 342 +R N LA+AQ L+ +V V Y GLE HP HAL++ F+ S +++F + +G + Sbjct: 298 DRITANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGG 357 Query: 343 FDYLNRLRLAIPTSNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDD 402 + + L+L N+GD ++L A T ++ G+ E +R+S+G+E DD Sbjct: 358 ARFQDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDD 417 Query: 403 LVADFRQALDA 413 L+AD QAL + Sbjct: 418 LLADLEQALQS 428 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 115 bits (288), Expect = 6e-27, Method: Compositional matrix adjust. Identities = 110/425 (25%), Positives = 178/425 (41%), Gaps = 50/425 (11%) Query: 1 MNDKKTYGFT--TTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRY 58 M+D K T T+++HS + + IH + T + D + ++ GY Y Sbjct: 1 MSDPKQTSLTPQTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTY 60 Query: 59 GRQGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNS 118 G G PT LE ++ ++E GK +G+AAI + L+ GD V+ ++G + Sbjct: 61 GLHGTPTSFVLEARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKD 120 Query: 119 L--WMTVGAQGAKVSMVDATDVKNVEAAITPNTRLVFVETIANPRTQVADLKRIGELCRE 176 L W+ Q D + + I P TRLV+ E + +V D+ + Sbjct: 121 LGQWLERDFQ-ITARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHA 179 Query: 177 RGILYVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEYDWTRYPHI 236 +G+L +DNT ++ F+ G +V+ ++TK G + L GA+ + Sbjct: 180 KGVLVAIDNTWSAGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAVIVRDQ--------- 230 Query: 237 AENYKKNPAPQWGMAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQERECKNALALAQ 296 A N K +A +R G +G + + + ++ LR E A +A Sbjct: 231 ALNEKLE------LAHMR------LGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAA 278 Query: 297 MLQADERVAAVYYPGLESHPQHALSKALFRSFGSLMSFELKDGIDCFD----------YL 346 L+ + AV +P E P HA K F G L S FD ++ Sbjct: 279 WLKQRPEIVAVLHPAFEDCPGHAHWKRDFSGAGGLFSV-------IFDPRYVEAQTDRFV 331 Query: 347 NRLRLAIPTSNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDDLVAD 406 + LRL + G +L +P Y M RRA M + L+R ++GLE +DL+AD Sbjct: 332 DSLRLFKIGYSWGGAHSLCVP------YRMKGMRRAWMREGQ-LVRFNIGLESPEDLIAD 384 Query: 407 FRQAL 411 QAL Sbjct: 385 IEQAL 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,312,940 Number of extensions: 56728 Number of successful extensions: 156 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 3 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)