BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0060.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 961 0.0 gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 99 1e-21 gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 80 5e-16 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 961 bits (2485), Expect = 0.0, Method: Compositional matrix adjust. Identities = 465/466 (99%), Positives = 466/466 (100%) Query: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV Sbjct: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 Query: 61 ADHNVPTTDRANGIADPISRLQVETLDGNAKEYSLTYFGMNDKRQGIVHVIGPEQGATLP 120 ADHNVPTTDRA+GIADPISRLQVETLDGNAKEYSLTYFGMNDKRQGIVHVIGPEQGATLP Sbjct: 61 ADHNVPTTDRAHGIADPISRLQVETLDGNAKEYSLTYFGMNDKRQGIVHVIGPEQGATLP 120 Query: 121 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA 180 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA Sbjct: 121 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA 180 Query: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL 240 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL Sbjct: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL 240 Query: 241 EYLKGRPFSPSGPHWERAVSYWRSLHSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMVVAV 300 EYLKGRPFSPSGPHWERAVSYWRSLHSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMVVAV Sbjct: 241 EYLKGRPFSPSGPHWERAVSYWRSLHSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMVVAV 300 Query: 301 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK 360 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK Sbjct: 301 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK 360 Query: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE Sbjct: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 Query: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL Sbjct: 421 PGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL 466 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 98.6 bits (244), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 113/396 (28%), Positives = 163/396 (41%), Gaps = 36/396 (9%) Query: 98 FGMNDKRQGIVHVIGPE---------QGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSE 148 FG+ GIVH + E G P T+V DSHT+ + G+G E Sbjct: 171 FGVVPPGFGIVHQVNLEYLARGVHNKTGVYYPD-TLVGTDSHTTMINGIGVVGWGVGGIE 229 Query: 149 VEHVLATQTLLARKSKAMLVQVDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTI 208 E + Q + + V + GAL GVTA D+VL + + G +EF G Sbjct: 230 AEAGMLGQPVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGT 289 Query: 209 RSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEYLKGRPFSPSGPHWERAVSYWRSLHSD 268 SLS+ R T+ NMA E GA G DD T+EY KG + + A + L+ Sbjct: 290 ESLSLTDRATIANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGV 349 Query: 269 PGA---KFDMVVTLNAAEIKPQVTWGTSPEMVVAV-DARVPDPDKEKDPTKRDGM-EKAL 323 P A + V+L+ + P + P+ + + + + D PT +G +KA Sbjct: 350 PKAGQIDYTQEVSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAE 409 Query: 324 AYMALKPNTPIEDIRIDKVFIG---SCTNSRIEDLREAAKVVRGKFRASNVKLA-----M 375 A N D+ V I SCTN+ + AA ++ K + +K+A Sbjct: 410 DLGASYKNADGVDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTS 469 Query: 376 VVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPGERCAS-------- 427 + PGS +V + E GL GF GC+ C+ NA L P A Sbjct: 470 LAPGSRVVTKYLEAAGLLPYLEKLGFGVTAYGCTTCIG-NAGDLTPAMNEAIVKNDVVAA 528 Query: 428 ---TSNRNFEGRQGAGGRTH-LVSPAMAAAAGIAGH 459 + NRNFE R R + L SP + A IAG+ Sbjct: 529 AVLSGNRNFEARIHPNIRANFLASPPLVVAYAIAGN 564 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 79.7 bits (195), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 105/381 (27%), Positives = 146/381 (38%), Gaps = 62/381 (16%) Query: 110 VIGPEQGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQ 169 VI + G P T+V DSHT A +A G+G E E+V+ + R + V+ Sbjct: 183 VIHAKDGVAFPD-TLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGVE 241 Query: 170 VDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGAR 229 + G G+TA DIVL++ + G +EF G SL++ R T+ NMA E GA Sbjct: 242 LTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGDRATISNMAPEYGAT 301 Query: 230 AGMVAFDDTTLEYLK--GRP---------FSPSGPHWERA---VSYWRSLHSDPGAKFDM 275 A M + D T++YLK GR ++ W V Y R L D Sbjct: 302 AAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAKVEYERVLRFD----LST 357 Query: 276 VVTLNAAEIKPQVTWGTSPEMVVAVDARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIE 335 VV A P S + +V E +P K + Sbjct: 358 VVRTLAGPSNPHKRLPVSELAAQGIAGKV-----ENEPGK------------------MP 394 Query: 336 DIRIDKVFIGSCTN-SRIEDLREAAKVVR-----GKFRASNVKLAMVVPGSGLVKEQAER 389 D + I SCTN S ++ A + R G R VK ++ PGS V+ E Sbjct: 395 DGAVIIAAITSCTNTSNPRNVIAAGLLARNANRLGLARKPWVKSSL-APGSKAVELYLEE 453 Query: 390 EGLDKIFRDAGFEWREPGCSMCLAMNADRLEPG-----------ERCASTSNRNFEGR-Q 437 GL GF C+ C M+ L+P + NRNF+GR Sbjct: 454 AGLTADLEKLGFGIVAFACTTCNGMSG-ALDPKIQQEIIDRDLYSTAVLSGNRNFDGRIH 512 Query: 438 GAGGRTHLVSPAMAAAAGIAG 458 + L SP + A IAG Sbjct: 513 PYAKQAFLASPPLVVAYAIAG 533 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,440,718 Number of extensions: 58791 Number of successful extensions: 187 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 3 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)