BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0057.p (629 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312599|ref|YP_003776691.1| major facilitator superfamily p... 1263 0.0 gi|300310049|ref|YP_003774141.1| 1-acyl-sn-glycerol-3-phosphate ... 61 3e-10 gi|300311697|ref|YP_003775789.1| acyltransferase family protein ... 58 2e-09 gi|300311464|ref|YP_003775556.1| major facilitator superfamily p... 57 4e-09 gi|300309811|ref|YP_003773903.1| 1-acyl-sn-glycerol-3-phosphate ... 56 9e-09 >gi|300312599|ref|YP_003776691.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 629 Score = 1263 bits (3269), Expect = 0.0, Method: Compositional matrix adjust. Identities = 628/629 (99%), Positives = 629/629 (100%) Query: 1 MQKNSQFTLFTQRRFAPFFWTQFLGALNDNVFKTALLTILTYDALSWTDLDPGLLNNLIP 60 MQKNSQFTLFTQRRFAPFFWTQFLGALNDNVFKTALLTILTYDALSWTDLDPGLLNNLIP Sbjct: 1 MQKNSQFTLFTQRRFAPFFWTQFLGALNDNVFKTALLTILTYDALSWTDLDPGLLNNLIP 60 Query: 61 GLFILPFFLFSATSGQLADKLEKGRVARFVKLLEIAIMGIAAYGWMTHHLWLLVAAVIGM 120 GLFILPFFLFSATSGQLADKLEKGRVARFVKLLEIAIMGIAAYGWMTHHLWLLVAAVIGM Sbjct: 61 GLFILPFFLFSATSGQLADKLEKGRVARFVKLLEIAIMGIAAYGWMTHHLWLLVAAVIGM 120 Query: 121 GVHSTLFGPVKYAYLPQQLRREELIGGNGLIEMGTFVGILLGEILGAVLVVHKPWGLHLV 180 GVHSTLFGPVKYAYLPQQLRREELIGGNGLIEMGTFVGILLGEILGAVLVVHKPWGLHLV Sbjct: 121 GVHSTLFGPVKYAYLPQQLRREELIGGNGLIEMGTFVGILLGEILGAVLVVHKPWGLHLV 180 Query: 181 AGMTIGIAVLGWLASLRIPLSPAPVPELKVNWNPFTETVRNLAYSRRNRPVFLSLLGNSW 240 AGMTIGIAVLGWLASLRIPLSPAPVPELKVNWNPFTETVRNLAYSRRNRPVFLSLLGNSW Sbjct: 181 AGMTIGIAVLGWLASLRIPLSPAPVPELKVNWNPFTETVRNLAYSRRNRPVFLSLLGNSW 240 Query: 241 FWFYGAIMLAQFPVYAKNYLHGDHSVFVLLLAVFSVGIGAGSLLCERLSGHKVEIGLVPF 300 FWFYGAIMLAQFPVYAKNYLHGDHSVFVLLLAVFSVGIGAGSLLCERLSGHKVEIGLVPF Sbjct: 241 FWFYGAIMLAQFPVYAKNYLHGDHSVFVLLLAVFSVGIGAGSLLCERLSGHKVEIGLVPF 300 Query: 301 GSIGLSVFGLELYFASQAYVTPTAMLDLGGFLAQGGSWRILIDCLGIGVFGGLYIVPLFA 360 GSIGLSVFGLELYFASQAYVTPTAMLDLGGFLAQGGSWRILIDCLGIGVFGGLYIVPLFA Sbjct: 301 GSIGLSVFGLELYFASQAYVTPTAMLDLGGFLAQGGSWRILIDCLGIGVFGGLYIVPLFA 360 Query: 361 LIQTRCDPAHVSRTIAGMNIMNALFMVVSALVAMVLLKAGLSIPQIFLATAIMNGVVAAY 420 LIQTRCDPAHVSRTIAGMNIMNALFMVVSALVAMVLLKAGLSIPQIFLATAIMNGVVAAY Sbjct: 361 LIQTRCDPAHVSRTIAGMNIMNALFMVVSALVAMVLLKAGLSIPQIFLATAIMNGVVAAY 420 Query: 421 IFSLVPEFLIRFLAWILIHTFYRVRIINGQAIPEQGPAVLVCNHVSYVDAIAIMAASPRP 480 IFSLVPEFLIRFLAWILIHTFYRVRIINGQAIPEQGPAVLVCNHVSYVDAIAIMAASPRP Sbjct: 421 IFSLVPEFLIRFLAWILIHTFYRVRIINGQAIPEQGPAVLVCNHVSYVDAIAIMAASPRP 480 Query: 481 VRFVMDHQIFRIPVMSWLFRNVRAIPIAPVKEDPWLTEKAFVDIAQALHEGELVCIFPEG 540 VRFVMDHQIFRIPVMSWLFRNVRAIPIAPVKEDPWLTEKAFVDIAQALHEGELVCIFPEG Sbjct: 481 VRFVMDHQIFRIPVMSWLFRNVRAIPIAPVKEDPWLTEKAFVDIAQALHEGELVCIFPEG 540 Query: 541 KLTRDGELNPFKGGVQKIIARSPVPVLPMALRGLWGSLFSRDPSNPVARTFKRGLFSRLE 600 KLTRDGELNPFKGGVQKIIARSPVPVLPMALRGLWGSLFSRDPSNPVARTFKRGLFSRLE Sbjct: 541 KLTRDGELNPFKGGVQKIIARSPVPVLPMALRGLWGSLFSRDPSNPVARTFKRGLFSRLE 600 Query: 601 LAVGQAIPPEQVSPELLQEKVRELRGQWK 629 LAVG+AIPPEQVSPELLQEKVRELRGQWK Sbjct: 601 LAVGEAIPPEQVSPELLQEKVRELRGQWK 629 >gi|300310049|ref|YP_003774141.1| 1-acyl-sn-glycerol-3-phosphate acyltransferase [Herbaspirillum seropedicae SmR1] Length = 267 Score = 61.2 bits (147), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 45/189 (23%), Positives = 83/189 (43%), Gaps = 28/189 (14%) Query: 443 RVRIINGQAIPEQGPAVLVCNHVSYVDAIAIMAASPRPVRFVMDHQIFRIPVMSWLFRNV 502 R I + +P+ P +L+ H S + I + PRP+ FV ++ +P W + Sbjct: 65 RYEIRGAENLPD-APVILLSKHQSAWETIFYVINMPRPLVFVFKKELTYVPFFGWGIALL 123 Query: 503 RAIPIAPVKEDPWLTEKAFVDI----AQALHEGELVCIFPEGKLTRDGELNPFKGGVQKI 558 R IPI K AF + + L +G+ + +FPEG T G +K G ++ Sbjct: 124 RMIPIDRKK-----GRDAFAQVLEHGTRRLRDGQWIMMFPEGTRTTVGSQGKYKAGGSRL 178 Query: 559 IARSPVPVLPMALRGLWGSLFSRDPSNPVARTFKRGLFSR----LELAVGQAIPPEQVSP 614 + V+P+A+ + + +G F + + +++G+ I PE ++P Sbjct: 179 AVETNTLVVPIAMNS--------------GQCWPKGSFIKKPGLITVSIGKPISPEGLNP 224 Query: 615 ELLQEKVRE 623 + L E+V Sbjct: 225 QELNERVEN 233 >gi|300311697|ref|YP_003775789.1| acyltransferase family protein [Herbaspirillum seropedicae SmR1] Length = 932 Score = 57.8 bits (138), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 50/160 (31%), Positives = 78/160 (48%), Gaps = 14/160 (8%) Query: 427 EFLIRFLAWILIHTFYRVRIINGQAIPEQGPAVLVCNHVSYVDAIAIMAASPRPVRFVMD 486 + L+R LA +V I +P GP VLV NH SY+D++ ++AA P + + Sbjct: 737 QLLVRLLA-------LQVSIAGTDHLP-SGPHVLVVNHSSYLDSLILLAALPPVPGYCLA 788 Query: 487 HQIFRIPVMSWLFRN-VRAIPIAPVKEDPWLTEKAFVD-IAQALHEGELVCIFPEGKLTR 544 + R W+ R +R + + V + VD + +AL GE + +FPEG TR Sbjct: 789 AK--RELEAHWIIRRFLRGLGVLFVDRSDISQSRTEVDSMVEALKRGENILVFPEGTFTR 846 Query: 545 DGELNPFKGGVQKIIARSPVPVLPMALRGLWGSLFSRDPS 584 + LN F G +++ VP++ LRG +L RD S Sbjct: 847 NAGLNAFHFGAFMAASKANVPLVVAGLRGTRLAL--RDES 884 >gi|300311464|ref|YP_003775556.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 423 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 95/377 (25%), Positives = 149/377 (39%), Gaps = 60/377 (15%) Query: 12 QRRFAPFFWTQFLGALNDNVFKTALLTILTYDALSWTDLDPGLLNNLIPGLFILPFFLFS 71 R F QF +L DN A + +L P + L+ F+L + L + Sbjct: 2 NRGFYTIMAAQFFSSLADNALLIAAIALLAEMH------SPAWMTPLLKLFFVLSYVLLA 55 Query: 72 ATSGQLADKLEKGRVARFVKLLEIAIMGIAAYGWMTHHLWLLVAAVIGMGVHSTLFGPVK 131 A G AD KG+V + I I+G + + H L L AV+G G + + P K Sbjct: 56 AFVGAFADAFPKGKVMLITNM--IKIVGCSLMFFTVHPL--LAYAVVGFG--AAAYSPAK 109 Query: 132 YAYLPQQLRREELIGGNGLIEMGTFVGILLGEILGAVLV---------------VHKPWG 176 Y L + L E+L+ NG IE T I++G +LG LV V P Sbjct: 110 YGILTELLPPEKLVAANGWIEGLTVGSIIMGTVLGGALVNPKISSVILSFDFPLVDLPID 169 Query: 177 LHLVAGMTIG--IAVLGWLASLRIPLSPAPVPELKVN----WNPFTETVRNLAYSRRNRP 230 A + I I V+ L + IP + A + N F L + + Sbjct: 170 TPTEAALCIISLIYVIAALFNFNIPDTGARYEHQERNPIRLIVDFAGCFTTLWKDKLGQ- 228 Query: 231 VFLSLLGNSWFWFYGAIMLAQFPV--YAKNYLHGDHSVFVLLLAVFSVGIGAGSLLCERL 288 +SL + FW GA + QF V +A+ LH +L + +VG+ G++ R Sbjct: 229 --ISLAVTTLFWGAGATL--QFIVLKWAEKSLHMPLDKAAILQGIVAVGVALGAMAAARF 284 Query: 289 SGHKVEIGLVPFGSIGLSVFGLELYFASQAYVTPTAMLDLGGFLAQGGSWRILIDCLGIG 348 K + ++P G V G+ + + T T++ W + +G Sbjct: 285 VPLKKSLTVMPLG----IVMGIVVMLMT----TTTSV------------WVAYPLLVIVG 324 Query: 349 VFGGLYIVPLFALIQTR 365 G ++VP+ AL+Q R Sbjct: 325 ALSGYFVVPMNALLQHR 341 >gi|300309811|ref|YP_003773903.1| 1-acyl-sn-glycerol-3-phosphate acyltransferase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 266 Score = 55.8 bits (133), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 41/129 (31%), Positives = 62/129 (48%), Gaps = 7/129 (5%) Query: 444 VRIINGQAIPEQGPAVLVCNHVSYVDAIAIMAASPRPVRFVMDHQIFRIPVMSWLFRNVR 503 +RI + IP ++LV NHVS++D A+ S +P RFV +I P++ WL Sbjct: 51 IRIDSPLPIPR---SLLVSNHVSWLDIFAVN--SLQPCRFVAKSEIRSWPLIGWLCAKTG 105 Query: 504 AIPIAPVKEDPWLTEKAFVDIAQALHEGELVCIFPEGKLTRDGELNPFKGGVQKIIARSP 563 I I+ K + F + +++ + E V FPEG G L PF + + + Sbjct: 106 TIFISRGKASD--VRRIFKGLVESIEKDEHVAFFPEGTTAAQGTLLPFHANLFEAAIDAK 163 Query: 564 VPVLPMALR 572 PV P ALR Sbjct: 164 APVQPYALR 172 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.449 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,998,976 Number of extensions: 88334 Number of successful extensions: 403 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 402 Number of HSP's successfully gapped: 5 Length of query: 629 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 538 Effective length of database: 1,188,985 Effective search space: 639673930 Effective search space used: 639673930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)