BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0050.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenas... 952 0.0 gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbas... 196 5e-51 gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked s... 157 2e-39 gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum ... 64 4e-11 gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur s... 55 9e-09 >gi|300312605|ref|YP_003776697.1| FAD/FMN-containing dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 952 bits (2460), Expect = 0.0, Method: Compositional matrix adjust. Identities = 471/475 (99%), Positives = 471/475 (99%) Query: 1 MRACAAAMNEFLQACRAAIGAAHVLTDAHDTAGYLTDQRGRYTGRALAVLRPADSAEVAA 60 MRACAAAMNEFLQACRAAIGA HVLTDAHDTAGYLTDQRGRYTGRALAVLRPADSAEVAA Sbjct: 1 MRACAAAMNEFLQACRAAIGAPHVLTDAHDTAGYLTDQRGRYTGRALAVLRPADSAEVAA 60 Query: 61 VVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGC 120 VVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGC Sbjct: 61 VVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGC 120 Query: 121 VLQDLQEQAAAAGRLFPLSLAAEGSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQ 180 VLQ LQEQAAAAGRLFPLSLAAEGSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQ Sbjct: 121 VLQHLQEQAAAAGRLFPLSLAAEGSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQ 180 Query: 181 GEIMSSLKGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQVTALAALPHPAQA 240 GEIMSSLKGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQVTALAALPHPAQA Sbjct: 181 GEIMSSLKGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQVTALAALPHPAQA 240 Query: 241 LQLLQLAQAHCGPALTGFELMSDFCLQLVRKHFPEQRLPFDRPHPQYVLLELSDSESEEH 300 LQLLQLAQAHCGPALTGFELMSDFCLQLVRKHFPEQRLPFDRPHPQYVLLELSDSESEEH Sbjct: 241 LQLLQLAQAHCGPALTGFELMSDFCLQLVRKHFPEQRLPFDRPHPQYVLLELSDSESEEH 300 Query: 301 ARTLFEGLIGTALEQGLVDDAVIAASLAQSRALWRLRESISSAQAHEGKNIKHDISVPIS 360 AR LFEGLIGTALEQGLVDDAVIAASLAQSRALWRLRESISSAQAHEGKNIKHDISVPIS Sbjct: 301 ARALFEGLIGTALEQGLVDDAVIAASLAQSRALWRLRESISSAQAHEGKNIKHDISVPIS 360 Query: 361 RIAEFIAATDVLVQQAAPGCRMVTFGHLGDGNLHYNVSPPEGVADSDFIARQAAINRVVH 420 RIAEFIAATDVLVQQAAPGCRMVTFGHLGDGNLHYNVSPPEGVAD DFIARQAAINRVVH Sbjct: 361 RIAEFIAATDVLVQQAAPGCRMVTFGHLGDGNLHYNVSPPEGVADRDFIARQAAINRVVH 420 Query: 421 DSVDRFGGSISAEHGLGALKREEITRYKSPVELQLMRAIKLALDPQQLMNPGKVL 475 DSVDRFGGSISAEHGLGALKREEITRYKSPVELQLMRAIKLALDPQQLMNPGKVL Sbjct: 421 DSVDRFGGSISAEHGLGALKREEITRYKSPVELQLMRAIKLALDPQQLMNPGKVL 475 >gi|300313123|ref|YP_003777215.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 196 bits (497), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 148/441 (33%), Positives = 222/441 (50%), Gaps = 40/441 (9%) Query: 48 AVLRPADSAEVAAVVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAV 107 AV+ + EVAA V+LC+Q+ P++P G T L G V QG V + L ++N++ AV Sbjct: 54 AVVFAHSTEEVAAFVKLCSQYDTPIIPYGAGTSLE-GHVLALQG-GVTVDLSQMNQVLAV 111 Query: 108 DALNNTITVESGCVLQDLQEQAAAAGRLFPLSLAAEGSCTIGGNLSTNAGGTGVLRYGNT 167 A + T TV++G + L ++ G FP+ A+ S +GG ST A GT +RYG Sbjct: 112 HAEDLTATVQAGVTRKQLNQEIKDTGLFFPIDPGADAS--LGGMASTRASGTNAVRYGTM 169 Query: 168 RELCLGLEVVTAQGEIMSSLKGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQ 227 +E L L VVTAQGEI+ + +K + GYDL +++G+EGTLG+IT ++L+PQP A Sbjct: 170 KENTLTLTVVTAQGEIIKTGTRAKKSSAGYDLTRVYVGSEGTLGIITEVTVRLYPQPEAI 229 Query: 228 VTALAALPHPAQALQLLQLAQAHCGPALTGFELMSDFCLQLVRKHFPEQRLPFDRPHPQY 287 A+ + P A A+ + + G L EL+ + ++ + H + LP + Sbjct: 230 SAAICSFPTVADAVNTV-IQTIQMGVPLARVELLDENGVRAINAH-DKLSLPVN----PL 283 Query: 288 VLLELSDSESEEHARTLFEGLIGTALEQGLVDD--------AVIAASLAQSRA-LWRLRE 338 +L E SE+ G + LV D A+ + R LW R Sbjct: 284 LLFEFHGSEN------------GVKEQAELVQDIAKEFHALGFEWATRPEDRTRLWTARH 331 Query: 339 SISSA--QAHEG-KNIKHDISVPISRIAEFIAATDVLVQQAAPGCRMVTFGHLGDGNLHY 395 + A Q G + I D VPISR+AE + AT ++ G GH+GDGN H Sbjct: 332 NAYFALLQLRPGARAISTDCCVPISRLAECVLATKADCEEQ--GLTHAIIGHVGDGNFHV 389 Query: 396 NVS-PPEGVADSDFIARQAAINRVVHDSVDRFGGSISAEHGLGALKREEITRYKSPVELQ 454 + P AD IAR +N+ + G+ + EHG+G K + + + + Sbjct: 390 QMMVDPNDPAD---IARAEGVNQRMVARAIGMDGTCTGEHGVGLHKMDFLIQEHGEGAIA 446 Query: 455 LMRAIKLALDPQQLMNPGKVL 475 +MRAIK ALDP+ +MNPGK+L Sbjct: 447 VMRAIKHALDPKNIMNPGKIL 467 >gi|300313121|ref|YP_003777213.1| glycolate oxidase (FAD-linked subunit) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 495 Score = 157 bits (397), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 135/472 (28%), Positives = 215/472 (45%), Gaps = 17/472 (3%) Query: 6 AAMNEFLQACRAAIGAAHVLTDAHDTAGYLTDQRGRYTGRALAVLRPADSAEVAAVVRLC 65 A E + A R + A VL D DT Y D Y + V+ P + A+V A+++ C Sbjct: 12 ARQREVVDALRRVVPAHCVLCDEEDTRPYECDGLAAYRQLPMVVVLPENEAQVVAIMKTC 71 Query: 66 AQFAVPLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGCVLQDL 125 V +VP+G TGL G+ P G VV+S + N+I +D + T V+ G + Sbjct: 72 HALQVQIVPRGAGTGLSGGATPIADG--VVVSTAKFNQILQMDKYSRTAVVQPGVRNLAI 129 Query: 126 QEQAAAAGRLFPLSLAAEGSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEIMS 185 E AA G + +++ +C+IGGN++ N+GG L+YG T L + +VT +GE++ Sbjct: 130 SEAAAPHGLYYAPDPSSQIACSIGGNVAENSGGVHCLKYGLTVHNVLRVRMVTIEGEVVE 189 Query: 186 SLKGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQVTALAALPHPAQALQ-LL 244 G D G DL +FIG+EG LGV+T +KL P+P+A +A+ + + Sbjct: 190 LGSGAL-DAPGLDLLAVFIGSEGMLGVVTEVTVKLVPKPQAARVIMASFDDVVKGGNAVA 248 Query: 245 QLAQAHCGPALTGFELMSDFCLQLVRKHFPEQRLPFDRPHPQYVLLELSDSESEEHARTL 304 + A PA G E+M ++V P + +D + +LL SD EE + Sbjct: 249 NVIAAGIIPA--GLEMMDKTSSRMVE---PFVKAGYDI-DAEAILLCESDGTVEEVEEEI 302 Query: 305 FEGLIGTALEQGLVDDAVIAASLAQSRALWRLRESISSAQAHEGKNIK-HDISVPISRIA 363 G + L + S A+ W R++ A + D ++P ++A Sbjct: 303 --GRMTDVLNASGATAIACSQSEAERLRFWSGRKNAFPAAGRISPDYYCMDGTIPRKKLA 360 Query: 364 EFIAATDVLVQQAAPGCRMVTFGHLGDGNLHYNVSPPEGVADSDFIARQAAINRVVHDSV 423 + + + ++ G R H GDGNLH + +AD R + + Sbjct: 361 QVLLGIAEMEKKY--GLRCANVFHAGDGNLHPLILFDANIADE--FHRAELFGAEILELC 416 Query: 424 DRFGGSISAEHGLGALKREEITRYKSPVELQLMRAIKLALDPQQLMNPGKVL 475 GG+I+ EHG+G K + SP E + +K A DP L+NP K + Sbjct: 417 VEVGGTITGEHGVGIEKINSMCVQFSPAERETFFKLKRAFDPAFLLNPDKAI 468 >gi|300313195|ref|YP_003777287.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 1333 Score = 63.5 bits (153), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 93/397 (23%), Positives = 151/397 (38%), Gaps = 78/397 (19%) Query: 36 TDQRGRYTGRALAVLRPADSAEVAAVVRLCAQFAVPLVPQGGNTGLVLGSVPDQQGNAVV 95 TD R Y VL P E+A +V+ C + + ++P+GG TG G++P +AV+ Sbjct: 188 TDWRVEY---PFVVLTPDTEDEMAGLVKACIELGLTIIPRGGGTGYTGGAIPLTPMSAVI 244 Query: 96 LSLRRLNRIRAV--------DALNNTITVESGCVLQDLQEQAAAAGRLFPLS-LAAEGSC 146 + +L ++ AV D TI +G V + + + A AG +F + +AE SC Sbjct: 245 -NTEKLEQLGAVEMEILPGLDKPYATIYSGAGVVTKRVSDAAEKAGFVFAVDPTSAEASC 303 Query: 147 TIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEIMS--------------------- 185 IGGN++ NAGG + +G + +V QG+ + Sbjct: 304 -IGGNVAMNAGGKKAVLWGTALDNLASWRMVDPQGDWLEVTRLDHNLGKIHDVEVARFKL 362 Query: 186 ----------------------SLKGLRKDNTGYDLRDLFIGA------EGTLGVITAAV 217 S K RK+ G D+ D F+ EG G+IT+A Sbjct: 363 EWSHPGEKGQKTEVFKTEILEISGKKFRKEGLGKDVTDKFLSGLPGVQKEGCDGLITSAR 422 Query: 218 MKLFPQPRAQVT--------ALAALPHPAQALQLLQLAQAHCGPALTGFELMSDFCLQLV 269 L P+ T A A+P + L G L G E + + L+ V Sbjct: 423 WILHKMPKQTRTVCLEFFGQARDAIPSIVEIKDYLDAETKKGGAILAGLEHLDERYLRAV 482 Query: 270 RKHFPEQRLPFDRPHPQYVLLELSDSESEEHARTLFEGLIGTALEQGLVDDAVIAASLAQ 329 +R P+ VL+ + E +I A V + +A S Sbjct: 483 GYATKSKRGVL----PKMVLIGDIVGDDENAVAAAASEVIRMA--NNRVGEGFVAVSPEA 536 Query: 330 SRALWRLRESISSAQAHEGK-NIKHDISVPISRIAEF 365 + W R ++ H I D+ +P++R+ E+ Sbjct: 537 RKKFWLDRSRTAAIAKHTNAFKINEDVVIPLNRMGEY 573 >gi|300313120|ref|YP_003777212.1| glycolate oxidase iron-sulfur subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 55.5 bits (132), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 46/162 (28%), Positives = 71/162 (43%), Gaps = 9/162 (5%) Query: 71 PLVPQGGNTGLVLGSVPDQQGNAVVLSLRRLNRIRAVDALNNTITVESGCVLQDLQEQAA 130 PL +GG T G P + +L R + I + IT G L ++ A Sbjct: 25 PLQIRGGGTKDWYGQTPSGE----LLDTRAYSGIIDYEPTELVITARCGTPLSEIDAALA 80 Query: 131 AAGRLF---PLSLAAEGSCTIGGNLSTNAGGTGVLRYGNTRELCLGLEVVTAQGEIMSSL 187 ++ P S G+ T+GG ++ G G R+ LG ++ AQGE + Sbjct: 81 QHNQMLAWEPPSFG--GAATVGGMVAAGLSGPARASAGAVRDFVLGAVLMDAQGEPLHFG 138 Query: 188 KGLRKDNTGYDLRDLFIGAEGTLGVITAAVMKLFPQPRAQVT 229 + K+ GYD+ L G+ GTLG+I +K+ P+P A T Sbjct: 139 GQVMKNVAGYDISRLLAGSLGTLGLILEVSLKVLPRPIASST 180 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,412,557 Number of extensions: 57234 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 5 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)