BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0048.p (695 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 1402 0.0 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 398 e-112 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 385 e-108 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 366 e-102 gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi... 363 e-101 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 1402 bits (3630), Expect = 0.0, Method: Compositional matrix adjust. Identities = 692/695 (99%), Positives = 694/695 (99%) Query: 1 MLIATYDKLLVVVSLLVAILASYTALDMAERINTTASQHGRAARWWLAGGAVAMGLGIWS 60 MLIATYDKLLVVVSLLVAILASYTALDMAERINTTASQHGRAARWWLAGGAVAMGLGIWS Sbjct: 1 MLIATYDKLLVVVSLLVAILASYTALDMAERINTTASQHGRAARWWLAGGAVAMGLGIWS 60 Query: 61 MHFIGMLAFRLPIALGYDAGITALSLVIAIAVSGFALWIVTRPELPTRRLLGSALFMGIG 120 MHFIGMLAFRLPIALGYDAGITALSLVIAIAVSGFALWIVTRPELPTRRLLGSALFMGIG Sbjct: 61 MHFIGMLAFRLPIALGYDAGITALSLVIAIAVSGFALWIVTRPELPTRRLLGSALFMGIG 120 Query: 121 IACMHYTGMAALRMQPGIAYDLFLFLASVAIAIAASAAALWIAFSLRRNTPHVKAARGGA 180 IACMHYTGMAALRMQPGIAYDLFLFLASVAIAIAASAAALWIAFSLRRNTPHV+AARGGA Sbjct: 121 IACMHYTGMAALRMQPGIAYDLFLFLASVAIAIAASAAALWIAFSLRRNTPHVRAARGGA 180 Query: 181 SVVMGIAIFGMHYTGMGAASFSRDSICLSAREGMAPGWLAVLIIVVTLAALTIALLTSVL 240 SVVMGIAIFGMHYTGMGAASFSRDSICLSAREG+APGWLAVLIIVVTLAALTIALLTSVL Sbjct: 181 SVVMGIAIFGMHYTGMGAASFSRDSICLSAREGVAPGWLAVLIIVVTLAALTIALLTSVL 240 Query: 241 DARLESRTAKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFAL 300 DARLESRTAKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFAL Sbjct: 241 DARLESRTAKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFAL 300 Query: 301 MFMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPED 360 MFMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPED Sbjct: 301 MFMDLDGFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPED 360 Query: 361 AMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGR 420 AMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGR Sbjct: 361 AMGVAEKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGR 420 Query: 421 NGYHFFEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHP 480 NGYHFFEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHP Sbjct: 421 NGYHFFEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHP 480 Query: 481 VHGLVAPDQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADP 540 VHGLVAPDQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADP Sbjct: 481 VHGLVAPDQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADP 540 Query: 541 HLLEVVRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYS 600 HLLEVVRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYS Sbjct: 541 HLLEVVRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYS 600 Query: 601 SLLYLKRLPANELKIDRGFVRELRQGGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDF 660 SLLYLKRLPANELKIDRGFVRELRQ GEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDF Sbjct: 601 SLLYLKRLPANELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDF 660 Query: 661 LTAAGCDALQGYLMGRPMTPELFLQTMRERHKSAA 695 LTAAGCDALQGYLMGRPMTPELFLQTMRERHKSAA Sbjct: 661 LTAAGCDALQGYLMGRPMTPELFLQTMRERHKSAA 695 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 398 bits (1023), Expect = e-112, Method: Compositional matrix adjust. Identities = 212/441 (48%), Positives = 285/441 (64%), Gaps = 9/441 (2%) Query: 254 SLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAIND 313 S+ EL D LT L NR +L L QAI A R++ ++F+DLD FK +ND Sbjct: 507 SMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVND 566 Query: 314 SLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPE-DAMGVA----EKL 368 +LGH GD LL VA RL ++ R DTVARLGGDEF++++ PE D + + +++ Sbjct: 567 TLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMIL----PEQDELTLDTRSLQRI 622 Query: 369 VEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEP 428 ++AV P + H L ++ SVG+A YP DG L+ +AD AMY K++GRN + F+ P Sbjct: 623 MDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTP 682 Query: 429 SMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPD 488 +MN A ++L+ DLR AL+ G+F L YQPK L +G V+G+EAL+RW+HP G+V+P Sbjct: 683 AMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPA 742 Query: 489 QFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRT 548 +FIGLAE +GLILPIGAWVL AC Q R+W GY D ++SVNLS QF L+ + Sbjct: 743 RFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIAD 802 Query: 549 TLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRL 608 LE LPP L LE+TES M D E ++ L + LGV +SIDDFGTGYSSL YLKR Sbjct: 803 VLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRF 862 Query: 609 PANELKIDRGFVRELRQGGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDA 668 P + LKIDR FVR++ +DAAI ++I++L R L LR++AEGVET EQ D+L GCD Sbjct: 863 PIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDE 922 Query: 669 LQGYLMGRPMTPELFLQTMRE 689 +QGYL P+ + F Q +R+ Sbjct: 923 IQGYLFSPPVPADKFEQILRQ 943 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 385 bits (989), Expect = e-108, Method: Compositional matrix adjust. Identities = 204/437 (46%), Positives = 280/437 (64%), Gaps = 7/437 (1%) Query: 261 ELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHF-ALMFMDLDGFKAINDSLGHHV 319 E+ HD LT LPNR +L + L +A+ + AR++G A+ F+DLD FK INDSLGH V Sbjct: 261 EMRHQATHDALTGLPNRNMLHEHLQRAL-RVARQKGALTAVAFVDLDQFKFINDSLGHQV 319 Query: 320 GDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPEDAMGVAE---KLVEAVNQPF 376 GD LL VAQRL + R D VAR GGDEFV+++ N E G+AE +++ AV +P+ Sbjct: 320 GDELLKTVAQRLQACLREADMVARQGGDEFVLVLQ-NQTEGEAGIAEVMQRILAAVARPW 378 Query: 377 KVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQ 436 + E +V+AS+G++ YP DG L+ +AD+AMY K GRN + FF P M++ Sbjct: 379 QAGDREFQVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSN 438 Query: 437 QLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAER 496 +L+ L +LR AL++ +F+L YQPK SL G ++GAEAL+RWE P G+V PD+FI AE Sbjct: 439 RLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEE 498 Query: 497 SGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLP 556 +GLI+PIG WVL ACRQ + W G ++VNLS Q + L + V L + L Sbjct: 499 AGLIVPIGEWVLRTACRQNQAWQQAGLPPIPVAVNLSPRQL-NQSLPDFVAGVLAQSGLA 557 Query: 557 PPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKID 616 L LE+TE+ M D E S+ TL + LG++IS+DDFGTGYSSL YL+R P + LKID Sbjct: 558 ASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKID 617 Query: 617 RGFVRELRQGGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGR 676 + FVR++ + + AAIV AI++L LNLR++AEGVE EQ FL CD +QGY G+ Sbjct: 618 KSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGK 677 Query: 677 PMTPELFLQTMRERHKS 693 PM +F M E+ ++ Sbjct: 678 PMEVAVFTAWMSEQAQA 694 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 366 bits (940), Expect = e-102, Method: Compositional matrix adjust. Identities = 187/431 (43%), Positives = 260/431 (60%), Gaps = 1/431 (0%) Query: 258 ANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGH 317 A+E + + +D+LT LPNR L ++ AI A G A++ MDLD FK IND+ GH Sbjct: 662 ADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNINDTFGH 721 Query: 318 HVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVD-LNTPEDAMGVAEKLVEAVNQPF 376 G+ +L E+AQR +S R D +AR+GGDEF++LVD + P +A+KL+ +PF Sbjct: 722 ETGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQLGEIAQKLLTEARKPF 781 Query: 377 KVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSMNANAQQ 436 +D E +S S+GIA YP DG L+ NAD AMY K G++ Y FF +N N + Sbjct: 782 IIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEINTNTVE 841 Query: 437 QLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAER 496 ++ +LR A+ER +F + YQPK L G ++GAEAL+RW+HP+ GL+ P +FI LAE Sbjct: 842 RIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEE 901 Query: 497 SGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLP 556 + LI IG VLD ACR + +S+NL+ QF D LL+ ++ ++RH P Sbjct: 902 TRLIDQIGLLVLDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGTP 961 Query: 557 PPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKID 616 P CL E+TES MH+ E ++ + I G ISIDDFGTGYSSL YLKR P N LKID Sbjct: 962 PSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKID 1021 Query: 617 RGFVRELRQGGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGR 676 + F+ ++ +AIV AI+A+ LNL++V EGVET +Q L G D QGY + Sbjct: 1022 KSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFSK 1081 Query: 677 PMTPELFLQTM 687 P+ F+Q + Sbjct: 1082 PVPYAQFVQLL 1092 >gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 706 Score = 363 bits (931), Expect = e-101, Method: Compositional matrix adjust. Identities = 205/435 (47%), Positives = 276/435 (63%), Gaps = 7/435 (1%) Query: 247 RTAKLARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLD 306 R L R + N++L HD LT LPNR LL DR+ A+ A R FA++FMDLD Sbjct: 243 RNQVLKRIVGRTNDKLLHFATHDVLTGLPNRALLADRIQHAVEVARRNGKTFAVLFMDLD 302 Query: 307 GFKAINDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILV-DLNTPEDAMGVA 365 GFKAINDSLGH VGD LLV VAQR+ R D VAR+GGDEFV++V +L++P+ ++ Sbjct: 303 GFKAINDSLGHAVGDGLLVAVAQRIRQCLRGEDMVARIGGDEFVVVVGNLSSPDVVEQLS 362 Query: 366 EKLVEAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHF 425 E ++ A+ Q F++D LRV++S+GIA+YP G + L+ NADAAMY K+SGRN Y F Sbjct: 363 ENILAALRQDFQIDDATLRVTSSIGIAVYPNSGDSVDALMKNADAAMYEAKQSGRNTYRF 422 Query: 426 FEPSMNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLV 485 FEP+M+A+A + LQ Q L+ A++ QFRL +QPK+ + + G EALLRW HP G + Sbjct: 423 FEPAMHASAMRHLQVRQALQQAIDEQQFRLHFQPKYRGASKELTGLEALLRWTHPQLGEM 482 Query: 486 APDQFIGLAERSGLILPIGAWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEV 545 AP +FI +AERSG IL IG WVL C Q+ W A G +++NLS LQ + + Sbjct: 483 APTEFIPIAERSGQILCIGDWVLQAVCEQIARWDAAGMKPVKVALNLSPLQMRTDLVARI 542 Query: 546 VRTTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYL 605 V + + P L E+TE+ AM D E S + + ++ LG +I+IDDFGTGYSSL YL Sbjct: 543 VE-LVGAAGIAPQRLMFEITETAAMKDVERSQRVIGELQALGFDIAIDDFGTGYSSLAYL 601 Query: 606 KRLPANELKIDRGFVRELRQGGEDAA---IVSAIVALGRTLNLRIVAEGVETVEQQDFLT 662 + ++KIDR F L G+D + IV+AI+AL L + +VAEGVET Q L Sbjct: 602 AQFHCRQIKIDRFFTSRL--DGDDHSGRAIVAAIIALAHALQMEVVAEGVETEGQLRELQ 659 Query: 663 AAGCDALQGYLMGRP 677 CD +QG+L+GRP Sbjct: 660 LLHCDQVQGFLLGRP 674 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,940,094 Number of extensions: 78911 Number of successful extensions: 540 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 456 Number of HSP's successfully gapped: 45 Length of query: 695 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 603 Effective length of database: 1,184,250 Effective search space: 714102750 Effective search space used: 714102750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 134 (56.2 bits)