BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0040.p (253 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 504 e-144 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 152 2e-38 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 148 4e-37 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 144 5e-36 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 142 3e-35 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 504 bits (1297), Expect = e-144, Method: Compositional matrix adjust. Identities = 251/253 (99%), Positives = 253/253 (100%) Query: 1 MLDLAGKVVFISGASTGIGAAAARAFAARGSHVVVHYNSSEKEAEAVAAEVRAAGSKAIT 60 MLDLAGKVVFISGASTGIGAAAARAFAARGSH+VVHYNSSEKEAEAVAAEVRAAGSK+IT Sbjct: 1 MLDLAGKVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSIT 60 Query: 61 IGADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSV 120 IGADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSV Sbjct: 61 IGADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSV 120 Query: 121 VAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADK 180 VAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADK Sbjct: 121 VAFTRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADK 180 Query: 181 IRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVT 240 IRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVT Sbjct: 181 IRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVT 240 Query: 241 GQIIDVNGGQYMP 253 GQIIDVNGGQYMP Sbjct: 241 GQIIDVNGGQYMP 253 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 152 bits (385), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 95/253 (37%), Positives = 146/253 (57%), Gaps = 6/253 (2%) Query: 2 LDLAGKVVFISGASTGIGAAAARAFAARGSHVVVHYNSSEKEAEAVAAEVRAAGSKAITI 61 +++A KV+ ++G S GIGAA A RG V V+Y ++ A+AV A+++AAG +AI + Sbjct: 1 MNMADKVILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAV 60 Query: 62 GADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVV 121 G DV + A ++GR+ L+NNAG L ++ LE ++ E + ++ N + Sbjct: 61 GGDVAREEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSL 120 Query: 122 AFTRAVIPVMRAQGGGN---IINVTSVAARHGGGPGALI-YAASKGFVSTLTRGMAKELL 177 R + M + GG I+N++S+AA+ GGPG + YAASKG + T G+AKE+ Sbjct: 121 LCAREAVKRMSTRHGGQGGAIVNLSSMAAKL-GGPGEYVDYAASKGAIDAFTIGLAKEVA 179 Query: 178 ADKIRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSG 237 A+ IRVNAV PG+I T +++ + +PM R G E + A L+L SE+ S Sbjct: 180 AEGIRVNAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEAS- 238 Query: 238 YVTGQIIDVNGGQ 250 Y TG IDV+GG+ Sbjct: 239 YATGTFIDVSGGR 251 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 148 bits (374), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 96/252 (38%), Positives = 151/252 (59%), Gaps = 9/252 (3%) Query: 4 LAGKVVFISGAS--TGIGAAAARAFAARGSHVVVHYNSSEKEAEAVAAEVRAAGSKAITI 61 L KV I+G + G+G + AR AA+G+ +V+ + AE A R G++ + + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVIL---DLERAEPAGAAARL-GAQHLGL 58 Query: 62 GADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVV 121 ADV D +A AA A + Q GRID+L+NNAG + + A L +T + +D I++++ R + Sbjct: 59 VADVTDKAACEAAAAAVLKQFGRIDILVNNAG-VTQPAKLLEITGKDYDRILDVSLRGTL 117 Query: 122 AFTRAVIPVMRAQGGGNIINVTSVAARHGGG-PGALIYAASKGFVSTLTRGMAKELLADK 180 ++AV+P M+AQ G+I+ ++SV+A+ GGG G Y+A+K V L R MA+E Sbjct: 118 YMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQG 177 Query: 181 IRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVT 240 IRVN + PG+I T + + E+ +TIP+GR+G PD+ G ++LAS+ +S Y T Sbjct: 178 IRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASD-LSQYCT 236 Query: 241 GQIIDVNGGQYM 252 G +DVNGG + Sbjct: 237 GVTLDVNGGMLI 248 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 144 bits (364), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 86/247 (34%), Positives = 135/247 (54%), Gaps = 4/247 (1%) Query: 4 LAGKVVFISGASTGIGAAAARAFAARGSHVVVHYNSSEKEAEAVAAEVRAAGSKAITIGA 63 LAGKV I+G +GIG A A A+A G+ + + Y E++A +V+A + + + Sbjct: 123 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 182 Query: 64 DVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAF 123 DV +AV + GR+DVL+NNA +E ++ + N S+ Sbjct: 183 DVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 242 Query: 124 TRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRV 183 + +P + G IIN TSV A + G + YAA+KG + TR ++++L + I V Sbjct: 243 VKEAMPFLTR--GARIINTTSVTA-YRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHV 299 Query: 184 NAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQI 243 NAVAPG I TP + E++ F K +P+GR G+PDE + A+++LA+E S Y+TGQ+ Sbjct: 300 NAVAPGPIWTPLIPASFSAEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGAS-YMTGQV 358 Query: 244 IDVNGGQ 250 + NGG+ Sbjct: 359 LHPNGGE 365 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 142 bits (358), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 96/252 (38%), Positives = 144/252 (57%), Gaps = 9/252 (3%) Query: 4 LAGKVVFISGAS--TGIGAAAARAFAARGSHVVVHYNSSEKEAEAVAAEVRAAGSKAITI 61 L KV I+G + G+G A AR A +G+ VVV + AA GS+ + + Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVL----DLAQADPAAAAARIGSQHLGL 58 Query: 62 GADVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVV 121 ADV D ++ A A + Q GRID+L+NNAG LE +T +D I++++ R + Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLE-ITGADYDRILDVSLRGTL 117 Query: 122 AFTRAVIPVMRAQGGGNIINVTSVAARHGGG-PGALIYAASKGFVSTLTRGMAKELLADK 180 ++AV+P MRAQ G+II ++SV+A+ GGG G Y+A+K V L R MA+E + Sbjct: 118 YMSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEG 177 Query: 181 IRVNAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVT 240 IRVN++ PG+I T + T E+ +TIP+ R+G D+ GA ++L S+ +S Y T Sbjct: 178 IRVNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSD-LSQYCT 236 Query: 241 GQIIDVNGGQYM 252 G +DVNGG + Sbjct: 237 GITLDVNGGMLI 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 638,363 Number of extensions: 24728 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 49 Length of query: 253 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 170 Effective length of database: 1,226,865 Effective search space: 208567050 Effective search space used: 208567050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)