BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0035.p (414 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 818 0.0 gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 421 e-119 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 92 6e-20 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 88 1e-18 gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 87 2e-18 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 818 bits (2113), Expect = 0.0, Method: Compositional matrix adjust. Identities = 413/414 (99%), Positives = 414/414 (100%) Query: 1 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 60 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS Sbjct: 1 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 60 Query: 61 AIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAV 120 AIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAV Sbjct: 61 AIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAV 120 Query: 121 GSVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPA 180 GSVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPA Sbjct: 121 GSVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPA 180 Query: 181 LLTLYIRRAISEPAVYLATQARLRETGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 240 LLTLYIRRAISEPAVYLATQARLRE+GQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS Sbjct: 181 LLTLYIRRAISEPAVYLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 240 Query: 241 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 300 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV Sbjct: 241 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 300 Query: 301 LVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 360 LVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV Sbjct: 301 LVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 360 Query: 361 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEAVAKAF 414 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEAVAKAF Sbjct: 361 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEAVAKAF 414 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 421 bits (1083), Expect = e-119, Method: Compositional matrix adjust. Identities = 216/410 (52%), Positives = 285/410 (69%) Query: 2 WLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSA 61 W ++ T ER+T A F+G+A+D D +++ IP L+AA+S+SKA+AG I+ L+ SA Sbjct: 4 WYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLVASA 63 Query: 62 IGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVG 121 IGGW AG L+DRYGRV+ LQ T++WF+FFTFLS F SF QL +A+QGFG GGEWA G Sbjct: 64 IGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEWAAG 123 Query: 122 SVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPAL 181 +VL+AETI A HRGKA G VQS+WAVGW G+ L F ++++LP E AW+V+F IG+LPAL Sbjct: 124 AVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLLPAL 183 Query: 182 LTLYIRRAISEPAVYLATQARLRETGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYSV 241 L +Y+RR++ E +LA Q L IF D+LR T++ SLL G G YY++ Sbjct: 184 LVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGYYAL 243 Query: 242 TTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIVL 301 TWLPT+LKTE+HLSVL T YL V+I +CG + S+ L D IGRRR FA + Sbjct: 244 MTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALACVAT 303 Query: 302 VLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAVG 361 VL Y + ++D ML GF LGFF +GI + MGA +EL+PS +RG+G GFCYNFGR Sbjct: 304 VLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGRVAS 363 Query: 362 AICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEAVA 411 A P LVG++S+S+PLGQ IG AA Y LV+VA L+LPET+G+++E A Sbjct: 364 AGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKMETSA 413 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 92.4 bits (228), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 110/433 (25%), Positives = 173/433 (39%), Gaps = 56/433 (12%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWT 83 D F + F++P L+ W +S A+ G + L IG G +A+RYGRV T Sbjct: 56 DAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVT 115 Query: 84 VLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 V + + + T SF LF R +QG G+GGE V + I E A RGK L + Sbjct: 116 VGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYEL 175 Query: 144 SWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPALLTLYIRRAISEPAVYLATQARL 203 + +G +A V A L P W+ +F +G +P +L + + E +L + RL Sbjct: 176 IFPLGLLAAA----QVGAFLVPRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRL 231 Query: 204 RETG--------------------------------------QRGNFLRIFHRDLLRTTL 225 + QRG++ +F T+ Sbjct: 232 DDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVYRGRTI 291 Query: 226 LASLLATGMQGAYYSVTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTI 285 + LL + WLP+ KT HL + + V S C L A L D + Sbjct: 292 IVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRV 351 Query: 286 GRRRCFMLFAAMGIVLVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGA-------YLS 338 GRRR + + L+ + AW + F S ++ MG Y Sbjct: 352 GRRRWAIASFILSGALLALLGVLKAESAWSVM------FLASSAYAVMGTTTVLLYLYTP 405 Query: 339 ELFPSDVRGSGQGFCYNFGRAVGAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLV 398 E++P+ +R G G ++ RA A PA+VG + + T+ + AG L ++A Sbjct: 406 EIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGHGIA-TVFLMFAGVTVLGLLAAWR 464 Query: 399 LPETRGKELEAVA 411 + ET + LE ++ Sbjct: 465 MIETTNRSLEEIS 477 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 112/416 (26%), Positives = 180/416 (43%), Gaps = 43/416 (10%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWT 83 D + F PT+ + ++ +A T + +G + G LADR GR VL Sbjct: 35 DAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGA 94 Query: 84 VLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 V+ A F S F F L R + G G+GG V ++AE + + RG+ +++S Sbjct: 95 VVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLES 154 Query: 144 SWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPALLTLYIRRAISEPAVYL------ 197 WA+G LA + AV AW+V+F++ LPAL+ + +R + E +YL Sbjct: 155 FWAIGTI--CLAVLALVAVSWGNDAWRVIFFVTGLPALVGVVLRFYVPESPMYLNRSGKS 212 Query: 198 --ATQARLR----------------ETGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYY 239 A Q R E +R +F L R +L L + AYY Sbjct: 213 DAARQVLERVAKVNGSSTPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISIAYY 272 Query: 240 SVTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRC---FMLFAA 296 V +LP L +E + +L+ L GY SA+ + GR+ F++ +A Sbjct: 273 GVFVYLPVKLGSE-GFGFMRGQIFLIFLALVQLPGYALSAYGVERWGRKPTLVGFLVLSA 331 Query: 297 MGIVLV-LSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYN 355 +G +L L M++ + +L F L G ++ + A+ E++P+D+R SG G Sbjct: 332 VGCMLYSLGKDPMLVVGSTLLLS-----FSLLGTWAALYAFTPEVYPTDLRASGMGTAGA 386 Query: 356 FGRAVGAICPALVGYLSNS---LPLGQTIGWIAAGCYGLVIVACLVLPETRGKELE 408 R G PA+V + S L L ++ AG ++ V E+R + LE Sbjct: 387 VARFGGLFAPAIVAPVMASHFTLALAMLSAFLVAGAVAILCVDV----ESRNRALE 438 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 101/384 (26%), Positives = 154/384 (40%), Gaps = 50/384 (13%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWT 83 DGFD ++ P ++A W +SKA G + L +G + DRYGR VL + Sbjct: 41 DGFDVQAMGYVAPAIIADWGVSKASLGPVFGAGLFGMLLGSLVLTPVGDRYGRRPVLILS 100 Query: 84 VLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 L+FA + T+ QL R + GFG+G L+ E + R LV Sbjct: 101 TLFFALCMLATPMVTTLDQLLVLRFITGFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSC 160 Query: 144 SWAVGWAGSALAFWGVYAVLPPETAWKVLFWI-GVLPALLTLYI--------------RR 188 + VG A V A L P W +FW+ G +P +L L + RR Sbjct: 161 GFTVGAAAGGF----VSAALIPAYGWHAVFWVGGAVPLVLGLAMLVWLPESIQFLVLHRR 216 Query: 189 AISEPAVYL-----------ATQARLRETGQRGNFLRIFHRD-LLRTTLLASLLATGMQG 236 ++ A +L T+ ++ET G + RD T+L L++ Sbjct: 217 PRTQVARWLRKLDPNIVIDDKTEVVVKETKAEGMPVAALFRDGRAGVTVLLWLISFMNLI 276 Query: 237 AYYSVTTWLPTFLKTERHLSVLGTSTYLLVLIG-----GSFCGYLTSAWLSDTIGRRR-- 289 Y ++ WLPT ++ G ST + VLIG G G L+ G R Sbjct: 277 NLYFLSNWLPTLIRDA------GYSTSMAVLIGTSLQVGGVIGTLSLGRFIQRFGFTRVL 330 Query: 290 --CFMLFAAMGIVLVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRG 347 CF+L A + I L+ ++ A + V GF + G + A +P+ +R Sbjct: 331 GSCFLL-ACLSIALI---GKLATVPALLFVAVIVAGFCIVGGQPAVNALAGTFYPTTLRS 386 Query: 348 SGQGFCYNFGRAVGAICPALVGYL 371 +G G+ GR + P + G L Sbjct: 387 TGIGWALGIGRIGSVVGPVIGGQL 410 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,300,028 Number of extensions: 56198 Number of successful extensions: 388 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 25 Length of query: 414 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 326 Effective length of database: 1,203,190 Effective search space: 392239940 Effective search space used: 392239940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)